Title: Spectrofluorometric Determination of Purine-Deoxyribonucleoside and Its Deoxyribonucleotides Using 4-Carbamoylbenzamidine
Abstract: A spectrofluorometric method utilizing 4-carbamoylbenzamidine as a new fluorogenic reagent selective for 2-deoxysugars is proposed for the determination of purine-deoxyribonucleos(t)ides. The method is based on the fluorescence reaction of 4-carbamoylbenzamidine with 2-deoxy-D-ribose released from purine-deoxyribonucleos(t)ides by acid hydrolysis. The optimized method is fairly selective; ribonucleos(t)ides and pyrimidine-deoxyribonucleos(t)ides yield only weak fluorescence of which intensities correspond to 0-15% that given by deoxyadenosine at an equimolar concentration. The method is also sensitive for purine-deoxyribonucleos(t)ides and DNAs; their detection limits are approximately 0.8 nmol/ml and 1.0 μg/ml, respectively.
Publication Year: 1994
Publication Date: 1994-04-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 3
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