Title: Stacking, derivatization, and separation by capillary electrophoresis of amino acids from cerebrospinal fluids
Abstract: ELECTROPHORESISVolume 27, Issue 10 p. 1922-1931 Research ArticleFull Access Stacking, derivatization, and separation by capillary electrophoresis of amino acids from cerebrospinal fluids Po-Ling Chang, Po-Ling Chang Department of Chemistry, National Taiwan University, Taipei, TaiwanSearch for more papers by this authorTai-Chia Chiu, Tai-Chia Chiu Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, TaiwanSearch for more papers by this authorHuan-Tsung Chang Professor, Corresponding Author Huan-Tsung Chang Professor [email protected] Department of Chemistry, National Taiwan University, Taipei, TaiwanDepartment of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 106, Taiwan Fax: +11-886-2-33661171===Search for more papers by this author Po-Ling Chang, Po-Ling Chang Department of Chemistry, National Taiwan University, Taipei, TaiwanSearch for more papers by this authorTai-Chia Chiu, Tai-Chia Chiu Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, TaiwanSearch for more papers by this authorHuan-Tsung Chang Professor, Corresponding Author Huan-Tsung Chang Professor [email protected] Department of Chemistry, National Taiwan University, Taipei, TaiwanDepartment of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei 106, Taiwan Fax: +11-886-2-33661171===Search for more papers by this author First published: 16 May 2006 https://doi.org/10.1002/elps.200500496Citations: 41AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract This paper describes the in-column derivatization, stacking, and separation of amino acids by CE in conjunction with light-emitting diode-induced fluorescence using naphthalene-2,3-dicarboxaldehyde (NDA). According to the relative electrophoretic mobilities and the migration direction in tetraborate solution (pH 9.3), the injection order is cyanide, then amino acids, then NDA. Once poly(ethylene oxide) (PEO) migrates through the capillary under EOF, the amino acid·NDA derivatives, amino acids, and CN− ions migrating against the EOF enter the PEO zone. As a result of increases in viscosity and possible interactions with PEO molecules, the reagents/analytes slow down such that they become stacked at the boundary. In comparison with the off-column approach to the analysis of amino acids, our proposed method provides a lower degree of interference from polymeric NDA compounds and other side products. As a result, the plot of the peak height as a function of γ-aminobutyric acid (GABA) concentration is linear over the range from 10−5 to 10−8 M, with the LOD being 4 nM. We demonstrate the diagnostic potential of this approach for the determination of amino acids, including GABA and glutamine, in biological samples through the analysis of large volumes of cerebral spinal fluids without the need for sample pretreatment. References 1 Hamase, K., Morikawa, A., Zaitsu, K., J. Chromatogr. B 2002, 781, 73–91. 2 Larson, A., Giovengo, S. L., Russell, I. L., Michalek, J. E., Pain 2002, 87, 201–211. 3 Shah, A. J., Crespi, F., Heidbreder, C., J. Chromatogr. B 2002, 781, 151–163. 4 Sethuraman, R., Lee, T. L., Tachibana, S., Clin. Chem. 2004, 50, 665–669. 5 Owens, D. F., Kriegstein, A. R., Nat. Rev. Neurosci. 2002, 3, 715–727. 6 Smith, A. J., Simpson, P. B., Anal. Bioanal. Chem. 2003, 377, 843–851. 7 Lee, I.-H., Pinto, D., Arriaga, E. A., Zhang, Z., Dovichi, N. J., Anal. 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