Title: Relationship between the Replication Functions of Streptomyces Plasmids pJV1 and pIJ101
Abstract: The essential replication region of the Streptomyces phaeochromogenes plasmid pJV1 was sequenced and compared to the equivalent region of the well-characterized Streptomyces plasmid pIJ101. The sequence revealed a similar organization in both plasmids, including a conserved region within both plasmid origins, which is just upstream of a gene encoding the replication protein (Rep). The Rep proteins of pJV1 and pIJ101 are very similar, and both have a conserved motif, also present in Rep proteins of single-stranded DNA plasmids of the low G + C gram+ bacteria that includes a tyrosine residue presumably involved in creating the single-stranded nick that initiates the replication event. A co-integration experiment between these two replicons led to the formation of a novel replicon with a hybrid origin and allowed the identification of the nick site where replication starts, also showing that the replication functions of both plasmids are related.
Publication Year: 1993
Publication Date: 1993-09-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 28
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