Title: Proteins Associated with α1‐Subunit‐Containing GABA<sub>A</sub> Receptors from Bovine Brain
Abstract: Abstract: In contrast to some other ion channels, there are at present no proteins known that bind specifically to mature GABA A receptor channels. Such proteins may be important for the structural organization and cytoskeletal anchoring of GABA A receptors and could also be expected to have channel modulatory effects. To identify proteins that are associated with naturally occurring GABA A receptors we immunoprecipitated these receptors from detergent‐solubilized bovine brain membranes by an antibody directed against the α1‐subunit. Tubulin and actin were observed to coprecipitate specifically with the receptors. Nine additional proteins were detected, hinting at a complex protein network associated with α1‐subunit‐containing GABA A receptors. Results of a biochemical characterization of these G ABA A receptor‐ t ubulin complex‐ a ssociated p roteins (GTAPs) are presented here. Peptide mass fingerprinting analysis and microsequencing of tryptic peptides indicated that at least three GTAPs have not been described until the present.
Publication Year: 1997
Publication Date: 1997-04-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 43
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