Publication Information

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278, 281, 301, 328, 338, 349, 356, 373, 384, 393, 411, 415, 432, 436, 494, 579, 679, 711, 738, 788, 810, 876, 925, 980, 1002, 1021, 1030, 1039, 1065, 1094, 1121, 1140, 1151, 1159, 1170, 1202, 1220, 1237, 1242, 1251, 1256, 1264, 1276, 1279, 1330, 1406, 1583, 1602, 1635, 1767, 1807, 1835, 1877, 1884, 1903, 1934, 1957, 2008, 2016, 2020, 2029, 2033, 2040, 2048, 2066, 2136, 2154, 2162, 2197, 2216, 2218, 2229, 2259, 2268, 2391, 2394, 2444, 2507, 2538, 2563, 2577, 2589, 2616, 2640, 2688, 2711, 2714, 2741, 2767, 2789, 2820, 2829, 2879, 2909, 3018, 3062, 3078, 3113, 3130, 3144, 3161, 3227, 3246, 3261, 3338, 3382, 3445, 3457, 3471, 3600, 3613, 3658, 3676, 3687, 3697, 3700, 3710, 3722, 3736, 3761, 3770, 3783, 3807, 3823, 3841, 3862, 3896, 3906, 3924, 3935, 4004, 4017, 4042, 4087, 4114, 4125], 'structure': [25, 184, 225, 245, 280, 439, 480, 500, 937, 1064, 1078, 1227, 2065, 2525, 2637, 2798, 2901, 2908, 3000, 3045, 3071, 3085, 3229, 3268, 3305, 3453, 3599, 3782, 3822, 3829, 3857, 3869, 3941], 'would': [29, 284, 1195, 1436, 1465], 'be': [30, 146, 167, 285, 401, 422, 664, 1089, 1181, 1336, 1813, 2624, 2649, 2809, 2833, 3067, 3593, 3692, 3715, 3740, 3747, 3968, 4010], 'expected': [31, 286], 'to': [32, 39, 63, 122, 242, 287, 294, 318, 377, 497, 779, 878, 886, 1041, 1069, 1073, 1549, 1588, 1623, 1629, 1646, 1649, 1661, 1698, 1800, 1812, 2059, 2190, 2400, 2521, 2612, 2634, 2648, 2667, 2693, 2700, 2719, 2747, 2796, 2822, 2832, 2835, 2899, 2905, 2930, 3069, 3083, 3093, 3099, 3157, 3266, 3272, 3288, 3293, 3311, 3321, 3399, 3403, 3451, 3552, 3557, 3595, 3621, 3667, 3764, 3819, 3835, 3855, 3875, 3911, 3927, 3953, 3987, 4009, 4046], 'shed': [33, 288], 'light': [34, 289], 'on': [35, 290, 1101, 1245, 1606, 2457, 3243], 'its': [36, 243, 291, 498, 1070, 1098, 1208, 1226, 1344, 1359, 2034, 3237, 3273, 3387, 3788], 'potential': [37, 292], 'applications': [38, 293], 'other': [40, 123, 149, 295, 378, 404, 2668, 2812, 3913, 3988, 4047], 'fields': [41, 296], 'such': [42, 250, 297, 505, 1433, 1462, 1825, 3603, 3607, 3727, 3928], 'as': [43, 298, 573, 749, 1379, 1777, 1826, 2025, 2304, 2332, 2351, 2379, 2405, 2491, 2839, 2988, 3309, 3495, 3525, 3728, 3749], 'detection': [45, 300, 2041], 'protease': [47, 302], 'activity.': [48, 303, 1099, 1145, 1293, 1317, 3903, 3995, 4055], 'Using': [49, 304], 'deletion': [50, 305, 1102, 1158, 1169, 1360, 1670, 1932, 2138, 2183, 2196, 2551, 2571, 2978, 3358, 3845, 3930], 'analysis,': [51, 306, 1103, 2572], 'we': [52, 307, 1231, 2004, 2112, 2573, 2662, 2682, 2784, 2817, 2993, 3088, 3256, 3325, 3571, 3609, 3650], 'have': [53, 308, 1820, 2401, 2453, 3651, 3866], 'defined': [54, 309, 1193, 1235], 'minimal': [56, 103, 311, 358, 1115, 1200, 1239, 1266, 1274, 1333, 1955, 2256, 2535, 2560, 3021, 3655], 'domain': [57, 68, 104, 312, 323, 359, 1116, 1241, 1275, 1334, 1956, 2537, 2562, 3022, 3657], 'in': [58, 127, 227, 313, 382, 482, 576, 666, 676, 816, 999, 1714, 1804, 1816, 1897, 1905, 2042, 2055, 2109, 2272, 2298, 2329, 2338, 2345, 2376, 2467, 2511, 2517, 2524, 2581, 2731, 2840, 2868, 2874, 2917, 2976, 3041, 3119, 3239, 3301, 3411, 3424, 3475, 3489, 3512, 3519, 3585, 3774, 3938, 3980, 4027, 4103], 'GFP': [59, 84, 130, 170, 182, 240, 253, 314, 339, 385, 425, 437, 495, 508, 661, 705, 751, 789, 805, 811, 927, 935, 1066, 1095, 1123, 1152, 1203, 1221, 1243, 1277, 1292, 1316, 1332, 1354, 1959, 2049, 2260, 2458, 2501, 2539, 2583, 2590, 2781, 2797, 2900, 2990, 3020, 3044, 3070, 3084, 3135, 3162, 3240, 3267, 3276, 3322, 3383, 3434, 3452, 3467, 3569, 3601, 3632, 3646, 3659, 3677, 3705, 3724, 3771, 3784, 3856, 3890, 3902, 3907, 3925, 3940, 3971, 3982, 3994, 4054, 4079, 4104], 'required': [60, 315, 1117, 1206, 1224, 2565, 3377, 3644], 'fluorescence': [62, 317, 675, 787, 817, 1120, 1209, 1916, 1925, 2037, 2153, 2160, 2189, 2207, 2277, 2318, 2365, 2404, 2426, 2459, 2475, 2490, 2518, 2614, 2726, 2828, 2987, 3112, 3129, 3362, 3508, 3538, 3580, 3633, 3789, 3905, 3926], 'amino': [64, 96, 124, 150, 164, 319, 351, 379, 405, 419, 713, 741, 774, 881, 1043, 1059, 1086, 1110, 1141, 1160, 1171, 1186, 1215, 1247, 1259, 1280, 1284, 1301, 1309, 1324, 1341, 1442, 1468, 1481, 1531, 1967, 1980, 2010, 2202, 2263, 2446, 2449, 2542, 2555, 2578, 2586, 2607, 2632, 2654, 2669, 2679, 2696, 2704, 2815, 2927, 3012, 3053, 3064, 3081, 3149, 3210, 3215, 3251, 3285, 3342, 3351, 3413, 3549, 3615, 3664, 3670, 3689, 3702, 3832, 3950, 3964, 3989, 4019, 4044, 4048, 4092, 4100], 'acids': [65, 97, 125, 151, 165, 320, 352, 380, 406, 420, 714, 742, 775, 1087, 1111, 1142, 1161, 1172, 1216, 1260, 1281, 1302, 1532, 1692, 1968, 1981, 2203, 2447, 2556, 2928, 3286, 3343, 3414, 3550, 3616, 3665, 3690, 3703, 3951, 3990, 4045, 4093], '7–229.': [66, 321, 1282], 'This': [67, 322, 872, 1077, 1829, 3960, 4021], 'starts': [69, 324, 2261], 'at': [70, 79, 98, 111, 134, 325, 334, 353, 366, 389, 740, 880, 888, 1025, 1050, 1217, 1261, 1287, 1300, 1308, 1326, 1343, 1438, 1467, 1480, 1651, 1961, 1974, 2262, 2541, 2595, 2600, 2678, 2772, 2859, 2922, 3014, 3032, 3148, 3209, 3214, 3253, 3837, 4063, 4091, 4099], 'middle': [72, 327], 'first': [75, 330, 3572], 'small': [76, 331, 1014, 1037, 3543, 3564, 3826, 3878, 3884], 'α': [77, 332, 989, 1015, 2920, 2972, 2998, 3544, 3827, 3850, 3879, 3885, 3899], 'helix': [78, 333, 990, 2921, 2973, 2999, 3828, 3851], 'N': [81, 113, 336, 368, 746, 1027, 1328, 1631, 1963, 2924, 3003, 3766, 3809, 3839], 'terminus': [82, 114, 137, 337, 369, 392, 747, 1150, 1329, 1346, 1964, 1977, 3004, 3017, 3675, 3769, 3812, 3840], 'ends': [86, 341, 1973, 2015, 2540, 3208], 'immediately': [87, 342], 'following': [88, 343, 3955], 'last': [90, 345, 3011, 3204, 3957, 4030], 'β': [91, 346, 983, 994, 1032, 3206, 3222, 3958, 4031], 'sheet.': [92, 347, 3959, 4032], 'Studies': [93, 348], 'both': [99, 354, 1190, 2014, 2740, 2819], 'termini': [100, 355, 1093, 1219, 1263, 1634], 'revealed': [105, 360], 'that': [106, 221, 248, 361, 476, 503, 933, 1084, 1109, 1204, 1222, 1272, 1297, 1322, 1434, 1463, 1501, 1585, 1819, 1953, 2053, 2180, 2254, 2442, 2504, 2515, 2554, 2605, 2765, 2786, 2855, 2995, 3058, 3258, 3391, 3442, 3546, 3653, 3684, 3713, 3758, 3848, 4059], 'positions': [107, 362, 1051, 2860, 4035], '6': [108, 363, 1303, 2186, 2234, 2775, 2861, 4036, 4065, 4094], '7': [110, 365, 1305, 2245, 2580, 2863, 3666, 4038, 4067, 4096], 'are': [115, 370, 1012, 1034, 1113, 1223, 1312, 2057, 2519, 2599, 2864, 2915, 3319, 3344, 3376, 3395, 3449, 3541, 3566, 3643, 3887, 4039], 'Glu-specific.': [116, 371, 4040], 'Change': [117, 372, 2710, 3456, 4041], 'Glu': [120, 375, 1298, 2593, 2622, 2665, 2676, 2691, 2717, 2743, 2770, 2792, 2806, 2857, 2913, 4061, 4089], 'residues': [121, 376, 1299, 2465, 2692, 2718, 2744, 2793, 2807, 2858, 2884, 2914, 4062, 4090], 'results': [126, 381, 2763, 3440, 3637, 3682, 3846, 4057], 'reduction': [128, 383], 'fluorescence.': [131, 157, 254, 386, 412, 509, 1355, 2567, 2584, 2782, 3009, 3455, 3647, 3680, 3872, 3891, 3974], 'Position': [132, 387], '229': [133, 388, 1311, 1486, 2421, 2451, 3152, 3217, 3952, 3977, 3998, 4102], 'C': [136, 391, 1149, 1345, 1633, 1976, 2432, 3016, 3768, 3811], 'GFP,': [139, 394, 519, 544, 2031, 2893, 2910, 3824], 'however,': [140, 395], 'nonspecific:': [142, 397], 'Ile': [144, 399, 1307, 3026, 3049, 3091, 3146, 3213, 3986, 4098], 'can': [145, 166, 400, 421, 663, 1088, 2808, 3691, 3967], 'replaced': [147, 402, 2810], 'with': [148, 152, 175, 403, 407, 430, 671, 812, 897, 1386, 1395, 1498, 1510, 1613, 1757, 1861, 1869, 1881, 1908, 1914, 2239, 2387, 2464, 2477, 2500, 2628, 2728, 2887, 3730], 'no': [153, 408, 3123, 3361, 3579], 'measurable': [154, 409], 'loss': [155, 410], 'A': [158, 413, 735, 1157, 1176], 'total': [159, 414, 3686, 3699], 'only': [161, 416, 1198, 1803, 2247, 2867, 3398, 4070], '15': [162, 417, 1056, 1600, 3688, 3701], 'terminal': [163, 418], 'deleted': [168, 423, 3345, 3573, 3693, 3969], 'without': [171, 426, 1096, 3972], 'disrupting': [172, 427], 'fluorescence,': [173, 428, 3570], 'consistent': [174, 429], 'findings': [176, 431], 'previous': [179, 434], 'study': [180, 435, 1270, 1933], 'crystal': [183, 244, 438, 499, 2046, 2907, 3159, 3274, 3821], '(Ormo,': [185, 440], 'M.,': [186, 441], 'Cubitt,': [187, 442], 'A.': [188, 443, 696, 1739, 1789], 'B.,': [189, 444], 'Kallio,': [190, 445], 'K.,': [191, 446], 'Gross,': [192, 447], 'L.': [193, 209, 448, 464, 858, 1844, 3793], 'A.,': [194, 449], 'Tsien,': [195, 450], 'R.': [196, 451, 605, 638, 685], 'Y.,': [197, 452], 'Remington,': [198, 453], 'S.': [199, 454, 695, 791, 823, 1738, 1788], 'J.': [200, 455, 603, 835, 902, 1125, 1939, 1986], '(1996)': [201, 216, 456, 471, 1107], 'Science': [202, 457], '273,': [203, 458], '1392–1395': [204, 459], 'Yang,': [206, 461], 'F.,': [207, 462], 'Moss,': [208, 463], 'G.,': [210, 465], 'Phillips,': [212, 467], 'G.': [213, 468, 588], 'N.,': [214, 469], 'Jr.': [215, 470], 'Nat.': [217, 472, 968, 2097, 2960, 3193], 'Biotechnol.': [218, 473, 969, 2098, 2961, 3194], '14,': [219, 474], '1246–1251)': [220, 475], 'tightly': [223, 478], 'packed': [224, 479], 'exists': [226, 481], 'protein.': [229, 484], 'We': [230, 485, 1294, 2178, 2211, 2824, 3389, 3405, 3577, 4108], 'also': [231, 486, 1080, 1211, 1295, 1488, 1674, 1922, 2173, 2212, 2873, 3402, 3432, 3630, 3746, 3909, 4074], 'generated': [232, 487, 3326], 'internal': [233, 488, 1351, 3328, 3882, 3914, 3929], 'deletions': [234, 251, 489, 506, 1139, 1188, 1286, 1352, 1680, 2012, 2390, 3400, 3672, 3865, 3893, 3915], 'within': [235, 490, 1017, 2892, 3336, 3617, 3916, 4077], 'loop': [237, 492, 3374, 3920], 'regions': [238, 493, 3296, 3318, 3375, 3394, 3620], 'according': [241, 496, 1548], 'found': [247, 502, 1296, 2179, 2225, 2825, 3126, 3390, 3578], 'all': [249, 504], 'eliminated': [252, 507], '1The': [515, 540], 'abbreviations': [516, 541], 'are:': [518, 543], 'Protein;': [522, 547], 'EGFP,': [523, 548, 1252, 2193, 2242], 'enhanced': [524, 549, 2018], 'GFP;': [525, 550], 'PCR,': [526, 551], 'polymerase': [527, 552, 1368], 'chain': [528, 553], 'reaction;': [529, 554], 'CHO,': [530, 555], 'Chinese': [531, 556], 'hamster': [532, 557], 'ovary;': [533, 558], 'FACS,': [534, 559], 'fluorescence-activated': [535, 560], 'cell': [536, 561], 'sorter;': [537, 562], 'Tc,': [538, 563], 'tetracycline.': [539, 564], 'jellyfishAequorea': [567], 'has': [569, 874, 1163, 2187, 2473, 2984], 'been': [570, 1821], 'reporter': [575, 3712, 3738], 'determination': [578], '(1Chalfie': [585], 'M.': [586, 625, 942, 1730, 1780, 2070, 2933, 3166], 'Euskirchen': [587], 'Ward': [589, 719, 761], 'W.W.': [590, 720, 762], 'Prasher': [591, 686, 755], 'D.C.': [592, 687, 716, 756], 'Science.': [593, 953, 2081, 2944, 3177], '1994;': [594, 697, 796], '263:': [595], '802-805Crossref': [596], 'PubMed': [597, 619, 656, 700, 730, 767, 799, 829, 850, 867, 917, 957, 973, 1133, 1569, 1743, 1793, 1852, 1947, 1994, 2085, 2102, 2948, 2965, 3181, 3198, 3801], 'Scopus': [598, 620, 631, 657, 701, 731, 768, 800, 830, 851, 868, 918, 958, 974, 1134, 1570, 1744, 1794, 1853, 1948, 1995, 2086, 2103, 2949, 2966, 3182, 3199, 3802], '(5466)': [599], 'Google': [600, 622, 633, 659, 703, 733, 770, 802, 832, 853, 870, 920, 960, 976, 1136, 1572, 1746, 1796, 1855, 1950, 1997, 2088, 2105, 2951, 2968, 3184, 3201, 3804], 'Scholar,': [601, 623, 634, 833, 854, 2089, 2952, 3185], '2Marshall': [602], 'Molloy': [604], 'Moss': [606, 963, 2092, 2955, 3188], 'G.W.': [607], 'Howe': [608], 'J.R.': [609], 'Hughes': [610], 'T.E.': [611], 'Neuron.': [612], '1995;': [613, 628, 650], '14:': [614, 971, 2100, 2963, 3196], '211-215Abstract': [615], 'Full': [616, 653, 845, 847, 912, 914], 'Text': [617, 654, 846, 848, 913, 915], 'PDF': [618, 655, 849, 916], '(220)': [621], '3Chalfie': [624], 'Photochem.': [626], 'Photobiol.': [627], '60:': [629], '651-656Crossref': [630], '(250)': [632], '4Cubitt': [635], 'A.B.': [636, 944, 2072, 2935, 3168], 'Heim': [637], 'Adams': [639], 'S.R.': [640, 860], 'Boyd': [641], 'A.E.': [642], 'Gross': [643, 947, 2075, 2938, 3171], 'L.A.': [644, 948, 2076, 2939, 3172], 'Tsien': [645, 688, 949, 2077, 2940, 3173], 'R.Y.': [646, 689, 950, 2078, 2941, 3174], 'Trends': [647], 'Biochem.': [648, 1848, 3797], 'Sci.': [649, 693, 1736, 1786], '20:': [651], '448-455Abstract': [652], '(1196)': [658], 'Scholar).': [660, 704, 734, 771, 803, 871, 921, 977, 1137, 1573, 1747, 1797, 1856, 1998, 2106, 2969, 3805], 'cDNA': [662, 1371, 1604], 'expressed': [665], 'various': [667], 'cells': [668, 1818, 1868, 1893, 1911, 1920, 2300, 2312, 2326, 2347, 2359, 2373, 2734, 3121, 3369, 3491, 3503, 3521, 3533, 3587, 3732], 'or': [669, 682, 1446, 1476, 1485, 2199, 2699, 2721, 2749, 2776, 3104, 3384, 3463, 3767, 3810, 3894], 'organisms': [670], 'an': [672, 1387, 2017, 2653, 2996, 3038], 'easily': [673], 'detected': [674], 'absence': [678, 1806], 'any': [680], 'substrate': [681], 'cofactor': [683], '(5Heim': [684], 'Proc.': [690, 1733, 1783], 'Natl.': [691, 1734, 1784], 'Acad.': [692, 1735, 1785], 'U.': [694, 1737, 1787], '91:': [698], '12501-12504Crossref': [699], '(1482)': [702], '27-kDa,': [708], 'single-chain': [709], 'polypeptide': [710], '238': [712], '(6Prasher': [715], 'Eckenrode': [717], 'V.K.': [718], 'Prendergast': [721, 759], 'F.G.': [722, 760], 'Cormier': [723], 'M.J.': [724], 'Gene': [725, 824, 1128, 1564, 1809, 1942, 1989], '(Amst.).': [726, 825, 1129, 1565, 1943, 1990], '1992;': [727, 1740, 1790], '111:': [728], '229-233Crossref': [729], '(1754)': [732], 'key': [736, 1214, 2170], 'sequence': [737, 1390, 1522, 1577, 1596, 1605, 2588], 'Ser-Tyr-Gly': [739], '65–67': [743], 'near': [744, 3001], 'functions': [748], 'fluorophore': [752], '(7Cody': [753], 'C.W.': [754], 'Westler': [757], 'W.M.': [758], 'Biochemistry.': [763], '1993;': [764], '32:': [765], '1212-1218Crossref': [766], '(572)': [769], 'These': [772, 1398, 1609, 2251, 2762, 2853, 3356, 3429, 4056], 'three': [773, 1013, 2114, 3115, 3407, 3430], 'undergo': [776], 'spontaneous': [777], 'oxidation': [778], 'form': [780, 3709, 3744], 'cyclized': [782], 'chromophore': [783, 997], 'responsible': [784], '(8Inouye': [790], 'Tsuji': [792], 'F.I.': [793], 'FEBS': [794], 'Lett.': [795], '351:': [797], '211-214Crossref': [798], '(133)': [801], 'Enhanced': [804], '(EGFP)': [806], 'mutant': [809, 930, 2184, 2422, 2485, 2831, 2979], '35-fold': [814, 2035], 'increase': [815], '(9Cormack': [818], 'B.P.': [819], 'Valdivia': [820], 'R.H.': [821], 'Falkow': [822], '1996;': [826, 842, 864, 909, 954, 970, 1130, 1849, 1944, 1991, 2082, 2099, 2945, 2962, 3178, 3195, 3798], '173:': [827, 1131, 1945, 1992], '33-38Crossref': [828], '(2499)': [831], '10Haas': [834], 'Park': [836, 903], 'E.C.': [837, 904], 'Seed': [838, 905], 'B.': [839, 906], 'Curr.': [840, 907], 'Biol.': [841, 908], '6:': [843, 910], '315-324Abstract': [844, 911], '(439)': [852, 919], '11Yang': [855], 'T.T.': [856], 'Cheng': [857], 'Kain': [859], 'Nucleic': [861], 'Acids': [862], 'Res.': [863], '24:': [865], '4592-4593Crossref': [866], '(349)': [869], 'variant': [873, 2983], 'mutations': [875, 1479, 1672, 2054, 2271, 2397, 2687, 3232, 3474, 3629, 3895, 3931], 'Ser': [877], 'Thr': [879], 'acid': [882, 1187, 1248, 1285, 1310, 1325, 1342, 1443, 1469, 1482, 2011, 2264, 2450, 2543, 2579, 2587, 2608, 2633, 2670, 2680, 3013, 3065, 3082, 3150, 3211, 3216, 3252, 3352, 3671, 3833, 3965, 3976, 4101], '65': [883], 'Phe': [885], 'Leu': [887, 3097], 'position': [889, 2644, 2774, 3034, 3151, 3263, 3997, 4006, 4064], '64': [890], 'encoded': [893], 'by': [894, 992, 1036, 1183, 1364, 1374, 1490, 1599, 1676, 1725, 1764, 1823, 1927, 2142, 2159, 2176, 2221, 2320, 2367, 2652, 2811, 3230, 3461, 3602], 'optimized': [898], 'human': [899], 'codons': [900], '(10Haas': [901], 'Recent': [922], 'crystallographic': [923], 'studies': [924, 1250, 1320, 3729, 3777], 'wild-type': [926, 1122, 1838, 1958, 2030], 'S65T': [931, 2051, 3164], 'indicate': [932, 1321, 2764, 3638, 3683, 3847, 4058], 'tertiary': [936, 3781], 'resembles': [938], 'barrel': [940, 2469], '(12Ormo': [941, 2069, 2932, 3165], 'Cubitt': [943, 2071, 2934, 3167], 'Kallio': [945, 2073, 2936, 3169], 'K.': [946, 2074, 2937, 3170], 'Remington': [951, 2079, 2942, 3175], 'S.J.': [952, 2080, 2943, 3176], '273:': [955, 2083, 2946, 3179], '1392-1395Crossref': [956, 2084, 2947, 3180], '(1909)': [959, 2087, 2950, 3183], 'Scholar,13Yang': [961], 'F.': [962, 2091, 2954, 3187], 'L.G.': [964, 2093, 2956, 3189], 'Phillips': [965, 2094, 2957, 3190], 'Jr.,': [966, 2095, 2958, 3191], 'G.N.': [967, 2096, 2959, 3192], '1246-1251Crossref': [972, 2101, 2964, 3197], '(1287)': [975, 2104, 2967, 3200], 'It': [978, 1798, 3755], 'consists': [979, 1278], '11': [981], 'antiparallel': [982, 3205], 'sheets': [984, 1033], 'single': [987, 1185, 1246, 2009, 2684, 3080], 'central': [988], 'surrounded': [991], 'sheets.': [995], 'resides': [998], 'center': [1001], 'barrel,': [1004], 'completely': [1005], 'shielded': [1006], 'extral': [1009], 'environment.': [1010], 'There': [1011, 2598, 3540, 3944], 'helices': [1016], 'protein,': [1019, 2408], 'one': [1020, 1040, 2198, 2713, 2766, 3335, 3423], 'which': [1022, 3282], 'located': [1024, 2466, 2916], 'terminus.': [1028], 'Most': [1029], 'connected': [1035], 'loops': [1038, 3448, 3937], 'four': [1042], 'acids.': [1044], 'Two': [1045], 'larger': [1046, 1168], 'connecting': [1047], 'loops,': [1048, 3281], 'beginning': [1049], '128': [1052], '188,': [1054], 'contain': [1055, 1502], '9': [1058, 2931], 'acids,': [1060, 2816, 4049], 'respectively.': [1061, 1543], 'compact': [1063], 'presumably': [1067], 'contributes': [1068], 'unusual': [1071], 'resistance': [1072], 'proteolysis': [1074], 'denaturation.': [1076], 'might': [1079, 2609, 2885, 3592, 3714], 'explain': [1081], 'observation': [1083], 'few': [1085], 'removed': [1090], 'affecting': [1097, 3973], 'Based': [1100, 3242], 'Dopf': [1104, 1935], 'Horiagon': [1106, 1126, 1937, 1940, 1987], 'concluded': [1108], '2–232': [1112], '(14Dopf': [1124, 1938, 1985], 'T.M.': [1127, 1941, 1988], '39-44Crossref': [1132, 1946, 1993], '(84)': [1135, 1949, 1996], 'N-terminal': [1138, 1420, 2115, 2182, 2570, 3844, 3864, 3877], '2–8': [1143], 'abolished': [1144], 'In': [1146, 1591, 1748, 3648, 3873, 4033], 'contrast,': [1147, 4034], 'slightly': [1154, 1167], 'less': [1155], 'critical.': [1156, 1349, 3155], '233–238': [1162], 'activity,': [1164, 3324, 3983], 'but': [1165, 1210, 2194, 2872, 3306, 3401, 3962, 3984, 4073], '226–238': [1173], 'does': [1174, 3233, 3991], 'not.': [1175], 'finer': [1177], 'map': [1178, 1236], 'could': [1179], 'likely': [1180, 4008], 'established': [1182], 'making': [1184], 'ends.': [1191], 'Such': [1192], 'analysis': [1194, 1255, 2223, 2267, 2331, 2378, 2499, 3470], 'provide': [1196], 'not': [1197, 1348, 2495, 2531, 2724, 2866, 3006, 3037, 3154, 3234, 3298, 3397, 3437, 3853, 3963, 3992, 4000, 4069], 'region': [1201, 2258, 3662], 'information': [1212], 'about': [1213], 'function.': [1229, 2800, 2903, 3047, 3073, 3241, 3270, 3943], 'Here': [1230], 'report': [1232], 'more': [1234, 2201, 4011], 'functional': [1240, 2257, 2536, 2561, 3656, 4085], 'based': [1244], 'truncation': [1249], 'substitution': [1254], 'several': [1257, 2169], 'important': [1258, 2795, 2865, 2898, 3068, 3265, 3450, 3979, 4012], 'fluorescent': [1267, 3323], 'domain.': [1268], 'Our': [1269, 1318, 3681, 3843], 'shows': [1271], 'Additional': [1283, 3669], 'either': [1288, 2712, 2773, 3379, 3460, 3674], 'end': [1289], 'dramatically': [1290, 2204, 2755, 3678], 'decrease': [1291, 3787], 'critical': [1313, 3007, 3320, 3854, 3888], 'maintaining': [1315, 2875, 3386, 3871, 3939, 3981, 4028], 'mutation': [1319], 'must': [1335], 'Glu,': [1337], 'while': [1338, 2243, 3870], 'specific': [1340, 2876, 3250, 4075], 'Furthermore,': [1350], 'eliminate': [1353, 3606], 'Wild-type': [1356], 'EGFP': [1357, 1370, 1603, 1665, 2022, 2056, 2269, 2429, 2564, 2730, 3472], 'mutants': [1361, 1545, 2139, 2156, 2220, 2846, 3116, 3132, 3359], 'were': [1362, 1402, 1487, 1546, 1586, 1618, 1641, 1673, 1696, 1708, 1859, 1879, 1912, 1921, 2140, 2296, 2313, 2327, 2343, 2360, 2374, 2385, 2398, 2509, 2745, 3365, 3487, 3504, 3517, 3534, 3583], 'made': [1363, 1547, 1675, 2005, 2113, 2141, 2683], 'PCR': [1365, 1494, 1508, 1621, 2143], 'withPfu': [1366], 'DNA': [1367, 1626, 1639, 1878], '(Stratagene).': [1369], 'was': [1372, 1427, 1456, 1495, 1509, 1528, 1578, 1597, 1658, 2023, 2157, 2172, 2753, 3117, 3125], 'amplified': [1373, 1408, 1706], 'using': [1375, 2301, 2348, 3492, 3522], 'plasmid': [1376], 'pEGFP-N1': [1377], '(CLONTECH)': [1378], 'template': [1381], '5′': [1384, 1431], 'primer': [1385, 1394, 1432, 1461], 'introduced': [1388, 1428, 1457, 1529, 1579], 'SacII': [1389, 1517], '3′': [1393, 1460], 'EcoRI': [1396], 'sequence.': [1397], 'two': [1399, 1610, 1625, 1638, 2200, 2602, 2660, 2690, 2716, 2769, 2791, 2805, 2881, 2912, 3279, 3327, 3357, 3393, 3410, 3447, 3542, 3563, 3575, 3619, 3640, 3883, 3898, 3918], 'restriction': [1400], 'sites': [1401, 1527], 'cloning': [1405], 'sequences': [1409, 1440], 'into': [1410, 1429, 1458, 1580, 1710, 1866, 2146, 3367], 'mammalian': [1412, 1715, 2043, 2148], 'vector': [1414, 1723, 2150], 'pTRE': [1415, 1711, 1717], '(CLONTECH).': [1416, 1828], 'To': [1417, 1448, 1999, 2656, 2801, 3074, 3314, 3559, 3605], 'make': [1418, 1449], 'deletions,': [1421, 1452, 2116, 2125, 3329], 'translation': [1423, 1435, 1464], 'initiation': [1424], 'codon': [1425, 1455], 'AUG': [1426], 'start': [1437], 'nucleotide': [1439], 'encoding': [1441], '7,': [1444, 1484, 2119, 2777], '8,': [1445], '9.': [1447], 'C-terminal': [1451, 2064, 2124, 2389, 2396, 2550], 'stop': [1454], 'terminate': [1466], '233,': [1470, 2127], '232,': [1471, 2128], '231,': [1472, 2129], '230,': [1473, 2130], '229,': [1474, 2131, 3966], '228,': [1475, 2132], '227.': [1477, 2134], 'Site-specific': [1478], '6,': [1483, 2118, 2681, 2981], 'created': [1489], 'two-step': [1491], 'PCR.': [1492], 'First-round': [1493], 'carried': [1496, 1814], 'out': [1497, 1815], 'primers': [1500, 1515, 1584, 1611, 1617], 'mutated': [1504, 1697, 2818, 3089, 3611], 'nucleotides,': [1505], 'second-round': [1507], 'EGFP-5′': [1512, 1614], '-3′': [1514, 1616], 'containing': [1516, 1666], 'andEcoRI': [1518], 'sites.': [1519], 'An': [1520], 'insertion': [1521, 1576, 3408], 'containingBglII': [1523], 'HindIII': [1525], 'recognition': [1526], 'between': [1530, 1966, 1979, 2658, 3127, 3412, 3425], '132': [1533, 3415], '133,': [1535], '137': [1536, 3419], '138,': [1538], '192': [1540, 3426], '193,': [1542], 'homology': [1551], 'extension': [1552], 'procedure': [1553, 2303, 2350, 3494, 3524], '(15Horton': [1554], 'R.M.': [1555], 'Hunt': [1556], 'H.D.': [1557], 'Ho': [1558], 'S.N.': [1559], 'Pullen': [1560], 'J.K.': [1561], 'Peasse': [1562], 'L.R.': [1563], '1989;': [1566], '77:': [1567], '61-68Crossref': [1568], '(2634)': [1571], 'First,': [1574, 2618], 'pair': [1582], 'complementary': [1587], 'each': [1589, 1607, 3337, 3612], 'other.': [1590], 'primers,': [1593], 'inserted': [1595], 'flanked': [1598], 'nucleotides': [1601], 'side.': [1608], 'along': [1612], 'amplification': [1622], 'generate': [1624], 'fragments,': [1627], 'corresponding': [1628], 'GFP.': [1636, 2021, 2617, 2641, 3695, 3842], 'fragments': [1640], 'then': [1642, 1659, 3610], 'denatured': [1643], 'renatured': [1645], 'allow': [1647], 'them': [1648, 2821], 'hybridize': [1650], 'their': [1652, 3458], 'homologous': [1653], 'sequences.': [1654], 'hybrid': [1656], 'molecule': [1657], 'extended': [1660], 'synthesize': [1662], 'full-length': [1664, 2192, 2241, 2407, 2428, 2479, 2989, 3134, 3723], 'insertion.': [1668], 'Internal': [1669], 'this': [1677, 1749, 2002, 2440, 2512, 2596, 2606, 2643, 2830, 2982, 3033, 3079, 3090, 3228, 3254, 3262, 3815, 3849, 3876, 4005], 'method.': [1678], 'include': [1681], 'Del': [1682, 1684, 1686, 1689, 3330, 3333, 3347, 3354], '[76–81],': [1683], '[83–88,': [1685], '[132–139],': [1687], '[191–195].': [1690, 3355], 'Amino': [1691, 3975], '71–81': [1693], '83–88': [1695], 'Ala,': [1699, 2698, 3622], 'Ala': [1700, 1703, 2720, 2748, 2842, 3623, 3626], '[73–81]': [1701], '[83–88].': [1704, 3627], 'DNAs': [1707], 'cloned': [1709, 2145], 'cells.': [1716, 2044, 2166], 'tetracycline': [1720], '(Tc)-inducible': [1721], 'developed': [1724], 'Gossen': [1726], 'Bujard': [1728, 1731, 1781], '(16Gossen': [1729, 1779], 'H.': [1732, 1782], '89:': [1741, 1791], '5547-5551Crossref': [1742, 1792], '(4232)': [1745, 1795], 'system,': [1751], 'modified': [1753], 'cytomegalovirus': [1754], '(CMV)': [1755], 'promoter': [1756, 1802], 'Tc-repressor': [1759], 'binding': [1760], 'element': [1761], 'regulated': [1763], 'fusion': [1766, 3760], 'tet-repressor': [1769], 'herpes': [1772], 'simplex': [1773], 'virus': [1774], 'VP-16,': [1775], 'designated': [1776], 'tTA': [1778], 'binds': [1799], 'Tc.': [1808], 'needs': [1811, 4105], 'transfected': [1822, 1865, 1919, 2164, 2273, 2732, 3366, 3476], 'tTA,': [1824], 'CHO/tTA': [1827, 1867, 2165, 2274, 2299, 2346, 2733, 3120, 3368, 3477, 3490, 3520, 3586], 'system': [1830], 'gives': [1831], 'higher': [1833, 2036], 'level': [1834], 'than': [1837, 2427, 2527, 4013], 'CMV-promoter': [1839], 'systems': [1840, 3718], '(17Ying': [1841, 3790], 'D.X.': [1842, 3791], 'Zhu': [1843, 3792], 'Schimke': [1845, 3794], 'R.T.': [1846, 3795], 'Anal.': [1847, 3796], '235:': [1850, 3799], '195-201Crossref': [1851, 3800], '(76)': [1854, 3803], 'constructs': [1858, 2171], 'purified': [1860], 'Qiagen': [1862], 'columns': [1863], 'liposome': [1871], 'transfection': [1872], 'reagent': [1873], '(CLONfectin,CLONTECH).': [1874], '5.0': [1875], 'μg': [1876, 1883], 'incubated': [1880], '5': [1882, 3350], 'CLONfectin': [1885], '30': [1887], 'min': [1888], 'overlaid': [1890], 'onto': [1891], '106': [1892], '1': [1895, 2209, 2416, 2431], 'h': [1896, 1902], 'serum-free': [1898], 'medium.': [1899], 'After': [1900, 2309, 2356, 3500, 3530], '16': [1901], 'growth': [1904], 'medium': [1907], 'serum,': [1909], 'examined': [1913, 3118, 3505, 3535, 3584], 'microscope.': [1917, 3509, 3539], 'analyzed': [1923, 2175, 2316, 2363], 'intensity': [1926, 2319, 2366], 'FACS': [1928, 2222, 2321, 2368], '(Becton': [1929, 2323, 2370], 'Dickinson).': [1930, 2324, 2371], 'Scholar)': [1951], 'showed': [1952, 3360], 'begins': [1960], 'somewhere': [1965, 1978], '2': [1969, 2708, 2736, 2757, 2848], '8': [1971, 2121], '226': [1982], '233': [1984], 'further': [2000, 4106], 'define': [2001], 'map,': [2003], 'series': [2007], 'version': [2019], 'target,': [2027], 'instead': [2028], 'because': [2032, 3860], 'ease': [2039], 'structures': [2047, 3160, 3444, 3565, 3642], 'suggest': [2052, 2854, 3441, 3932], 'unlikely': [2058], 'affect': [2060, 2456, 2725, 3993], 'N-': [2062, 2548], '13Yang': [2090, 2953, 3186], 'As': [2107], 'shown': [2108, 2399], 'Fig.1': [2110], 'A,': [2111], 'δ': [2117, 2126, 2185, 2233, 2244, 2411, 2420, 2471, 2483, 2492, 2980], 'seven': [2123], 'All': [2135, 2393], 'these': [2137, 2155, 2219, 2547, 2659, 2689, 2715, 2768, 2790, 2804, 2880, 2911, 3114, 3317, 3339, 3373, 3392, 3446, 3562, 3574, 3618, 3639, 3897, 3936, 4043], 'pTRE.': [2151], 'determined': [2158, 2213, 3652], 'microscopy': [2161], 'transiently': [2163], 'Expression': [2167], 'quantitatively': [2174], 'FACS.': [2177], 'similar': [2188, 2505], 'additional': [2195], 'reduced': [2205, 2754, 2834, 3679], '(Fig.': [2208, 2415, 2430, 2707, 2735, 2756, 2847], 'B).': [2210, 2737], 'activity': [2215, 2237, 2249, 2752, 3435], 'some': [2217, 2443, 3762, 3861], 'that,': [2226], 'after': [2227, 2448, 3363], 'subtraction': [2228], 'autofluorescence': [2231], 'background,': [2232], 'exhibited': [2235], '112%': [2236], 'compared': [2238, 2476, 2727], 'retained': [2246], '12%': [2248], '(TableI).': [2250, 2991], 'data': [2252], 'indicated': [2253, 2503], '7.Table': [2265], 'IFACS': [2266], 'cellsEGFP': [2275, 3478], 'mutationsRelative': [2276], 'intensity%': [2278], 'ControlIntact': [2279], 'EGFP610100%N-Terminal': [2280], 'Deletions': [2281, 2284], 'Del6683112%': [2282], 'Del77612%C-Terminal': [2283], 'Del2291055173%': [2285], 'Del22834456%N-Terminal': [2286], 'Mutations': [2287, 2291], 'A6E/A7E34657%': [2288], 'K6E/K7E20433%': [2289], 'D6E/D7E33455%C-Terminal': [2290], 'L229I696114%': [2292], 'K229I670110%': [2293], 'D229I54289%Negative': [2294], 'Control264%Transfections': [2295], 'performed': [2297, 2344, 2386, 3488, 3518], 'described': [2305, 2352, 3496, 3526], 'under': [2306, 2353, 3497, 3506, 3527, 3536], '"Experimental': [2307, 2354, 3498, 3528], 'Procedures."': [2308, 2355, 3499, 3529], '24': [2310, 2357, 3501, 3531], 'h,': [2311, 2358], 'collected': [2314, 2361], 'Caliber': [2322, 2369], 'Untransfected': [2325, 2372], 'included': [2328, 2375], 'negative': [2334, 2381, 2455, 2787, 2870, 4071], 'control.': [2335, 2382], 'Open': [2336, 3510], 'table': [2337, 3511], 'new': [2340, 3514], 'tab': [2341, 3515], 'Transfections': [2342, 3516], 'Similar': [2383], 'analyses': [2384, 2552], 'EGFP.': [2392], 'same': [2403, 2837, 2986], 'except': [2409], '228': [2412, 2472, 2493], '227': [2414, 2484], 'C).': [2417], 'Surprisingly,': [2418], 'exhibits': [2423, 2486], 'brighter': [2425], 'Table': [2434, 2760, 2851, 3139], 'I).': [2435, 2482, 2761, 2852, 3140], 'One': [2436, 2878], 'possible': [2437, 2603, 3057], 'explanation': [2438], 'may': [2452, 2623, 2645, 3066, 3739, 3745, 3778, 3813, 4023], 'via': [2460], 'non-favorable': [2462], 'interaction': [2463, 2627, 2896, 3031], 'structure.': [2470, 3388, 4080], '56%': [2474], '(Table': [2481, 3370, 3588, 3634], 'similarly': [2488], 'declined': [2489], '(data': [2494, 2530, 3436], 'shown).': [2496, 2532, 3438], 'Western': [2497], 'blot': [2498], 'antibodies': [2502], 'amounts': [2506], 'study,': [2513], 'suggesting': [2514], 'variations': [2516], 'due': [2520, 3594], 'difference': [2523, 3124], 'rather': [2526, 3307], 'levels': [2529], 'Therefore,': [2533, 2783, 2992, 3141, 3372], '229.': [2544, 3668], 'Taken': [2545], 'together,': [2546], 'reveal': [2553], '7–229': [2557], 'comprise': [2558], 'From': [2568], 'know': [2574], 'importance': [2576, 3238, 3934], 'determining': [2582, 3042], 'Primary': [2585], 'indicates': [2591], 'residue': [2594, 2666, 2677, 2891, 3092, 3096, 3147], 'position.': [2597], 'least': [2601], 'ways': [2604], 'contribute': [2610, 4068], 'significantly': [2611], 'properties': [2615], 'negatively': [2620, 2813, 2882, 3106], 'charged': [2621, 2631, 2703, 2814, 2883, 2890, 3102, 3107], 'needed': [2625], 'another': [2629, 2675, 3094], 'oppositely': [2630], 'maintain': [2635], 'function': [2639, 3859], 'Alternatively,': [2642], 'simply': [2646], 'need': [2647], 'physically': [2650], 'occupied': [2651], 'acid.': [2655, 4020], 'distinguish': [2657], 'possibilities,': [2661], 'changed': [2663, 2746, 3597], 'residues.': [2671], 'Because': [2672], 'there': [2673], 'double': [2686, 2845], 'neutral': [2695, 3025], 'acid,': [2697, 2705, 3054], 'positively': [2702, 2889, 3101], 'Lys': [2706, 2722, 2844, 3103], 'A).': [2709, 3110], 'did': [2723], 'unmodified': [2729], 'However,': [2738], 'when': [2739, 3581], 'Lys,': [2750], 'B': [2758, 2849, 3137], 'residues,': [2771], 'essential': [2779, 3039], 'conclude': [2785, 2994, 3257], 'charge': [2788, 3030, 4072], 'test': [2802, 3560], 'if': [2803, 3316, 3561], 'Asp.': [2823], 'green': [2827], 'degree': [2838], 'providing': [2869], 'charges': [2871], 'contacts.': [2877], 'interact': [2886], 'According': [2904, 3156, 3271, 3818], 'short': [2919, 3947], 'terminus,': [2925], '3': [2929, 3834], 'Although': [2970], 'destroyed': [2975, 3631, 3867], 'intact': [2997], 'residue.': [3027], 'Thus,': [3028], 'ionic': [3029], 'probably': [3036], 'factor': [3040], 'Since': [3048], 'hydrophobic': [3052, 3060, 3095], 'it': [3055], 'character': [3061], 'determine': [3075, 3315], 'contribution': [3077], 'function,': [3087], 'Asp': [3108], '(Fig.3': [3109, 3136], 'hydrophobicity': [3143], 'Scholar),': [3202], 'sheet': [3207], '228.': [3212], 'excluded': [3219], 'sheet,': [3223], 'so': [3224], 'disturbance': [3226], 'account': [3235], 'lack': [3245], 'preference': [3247], 'position,': [3255], 'physical': [3259], 'occupancy': [3260, 4003, 4022], 'structure,': [3275], 'contains': [3277], 'largest': [3280, 3919], 'locate': [3283], '129': [3287], '142': [3289], '189': [3292], '196.': [3294], 'do': [3297], 'directly': [3299], 'participate': [3300], 'forming': [3302], 'β-can': [3304], 'serve': [3308], '"lid"': [3310], 'barrel.': [3313], '[132–139]': [3331, 3348], '[191–195],': [3334], 'regions.': [3340, 3921], 'Eight': [3341], 'they': [3364, 3582], 'II).': [3371, 3589, 3635], 'proper': [3380], 'folding': [3381], 'sensitive': [3396, 3910], 'insertions.': [3404], 'chose': [3406], 'sites,': [3409], '133': [3417], '138': [3421], '193.': [3428], 'insertions': [3431], 'inactivate': [3433], 'length,': [3459], 'shortening': [3462], 'enlarging,': [3464], 'will': [3465], 'destroy': [3466, 3901], 'fluorescence.Table': [3468], 'IIFunction': [3469], 'mutationsFluorescenceIntact': [3479], 'EGFP+Del': [3480], '[132–139]−Del': [3481], '[191–195]−Del': [3482], '[76–81]−Del': [3483], '[83–88]−Ala': [3484], '[76–81]−Ala': [3485], 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