Title: L-Type and Dihydropyridine-Resistant Calcium Channel Trigger Exocytosis with Similar Efficacy in Single Rat Pancreatic β Cells
Abstract: The relationship between depolarization-induced exocytosis and inward Ca2+current (ICa) in single isolated rat pancreatic β cells was investigated in perforated patch recordings.ICawas elicited by depolarization and change in cell membrane capacitance (Cm) was monitored as an indicator of resultant exocytosis. While there was significant variety of change inCmand Ca2+influx, the increase inCmhad positive correlation with Ca2+influx and also with duration of depolarization. Removal of extracellular Ca2+or inclusion of extracellular Cd2+(100 μM) completely eliminated bothICaand increase inCmfollowing depolarization. Dihydropyridine (DHP) Ca2+channel blocker (5 μM) partly and in parallel suppressed depolarization-induced peakICa, Ca2+influx, and change inCm. These data suggest that rat pancreatic β cell expresses at least two types of Ca2+channels; Ca2+entry through these DHP-sensitive, presumably L-type, and DHP-insensitive channels triggers exocytosis with similar efficacy.
Publication Year: 1998
Publication Date: 1998-02-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 7
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