Title: Early interactions of foot-and-mouth disease virus with cultured cells
Abstract: The adsorption of foot-and-mouth disease virus (FMDV) types A12119, O1B, and C3Res were studied in baby hamster kidney (BHK-21) cells by measuring the amount of radioactively labeled purified virus which remained attached to cells at various times after infection. At 4°, over half of the maximum adsorption of type A virus occurred within 15 min, and approximately 65% of the radioactivity bound by 45 min was removed by brief treatment with EDTA, indicating the assay measures primarily adsorption and not penetration. Upon shifting the temperature from 4 to 37°, about 60 to 70% of the bound virus eluted by 1 hr in an unmodified form. Only 140 S virus particles and 12 S subunits could be found associated with the infected cell 20 min after shifting the temperature from 4 to 37°. By 50 min, the number of 140 S particles decreased slightly. The number of viral receptors per BHK-21 cell was determined to be 1–2.5 × 104 for virus types A12 and O1B. Competition experiments between FMDV types A12, O1B, and C3Res indicated that they can utilize at least some common receptor sites on cells.
Publication Year: 1980
Publication Date: 1980-07-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 75
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