Title: Induction of apoptosis in cultured cells by extracts from shiitake (Lentinula edodes) mycelial culture broth
Abstract: Extracts from shiitake (Lentinula edodes) mycelial culture broth, by an organic solvent ethyl acetate, inhibited the proliferation of cultured cells. At lower concentrations (1.25-15 μg/ml), this inhibition, measured by the MTT assay, was dose- and cell line-dependent, inhibition of tumor cells, such as Caski, SiHa, HeLa, HP-1 and A375, by L. edodes- 436 extracts was stronger than inhibition of normal cells (3T3). At 20 μg/ml, the extracts induced changes in cell shape, DNA-fragmentation and the activation of caspase-3. The extracts also inhibited the binding of E2F protein to its promoter. The results suggest that extracts of L. edodes culture broth contain substances that have the ability to induce apoptosis in the cultured cells.
Publication Year: 2000
Publication Date: 2000-12-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 2
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