Title: Tissue culture of forest trees—Clonal propagation of mature trees of Eucalyptus citriodora Hook, by tissue culture
Abstract: Multiple shoots were obtained from terminal buds of 20-year-old trees of Eucalyptus citriodora Hook on Murashige and Skoog's medium supplemented with calcium pantothenate, biotin, benzylaminopurine and kinetin. Rooting could be induced by naphthalene-acetic acid in shoot cultures only after they had undergone three subcultures. Incubation at 15°C with continuous illumination followed by growth in agitated liquid cultures was essential for inducing shoot development in the primary terminal buds. These treatments were not necessary in later subcultures or with explants from seedlings obtained from seeds. Fifteen subcultures have so far been carried out and healthy viable plantlets obtained in each subculture. It is estimated that over 100 000 plants can be obtained by this method in a year from a single bud of mature Eucalyptus citriodora trees.
Publication Year: 1981
Publication Date: 1981-01-01
Language: en
Type: article
Indexed In: ['crossref']
Access and Citation
Cited By Count: 105
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