Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2003343658', 'doi': 'https://doi.org/10.1074/jbc.271.49.31044', 'title': 'Negative Dominance Studies Demonstrate the Oligomeric Structure of EmrE, a Multidrug Antiporter from Escherichia coli', 'display_name': 'Negative Dominance Studies Demonstrate the Oligomeric Structure of EmrE, a Multidrug Antiporter from Escherichia coli', 'publication_year': 1996, 'publication_date': '1996-12-01', 'ids': {'openalex': 'https://openalex.org/W2003343658', 'doi': 'https://doi.org/10.1074/jbc.271.49.31044', 'mag': '2003343658', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/8940098'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.271.49.31044', 'pdf_url': 'http://www.jbc.org/article/S0021925819789510/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'hybrid', 'oa_url': 'http://www.jbc.org/article/S0021925819789510/pdf', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5081992488', 'display_name': 'Hagit Yerushalmi', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I197251160', 'display_name': 'Hebrew University of Jerusalem', 'ror': 'https://ror.org/03qxff017', 'country_code': 'IL', 'type': 'education', 'lineage': ['https://openalex.org/I197251160']}], 'countries': ['IL'], 'is_corresponding': False, 'raw_author_name': 'Hagit Yerushalmi', 'raw_affiliation_strings': ['From the Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel'], 'affiliations': [{'raw_affiliation_string': 'From the Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel', 'institution_ids': ['https://openalex.org/I197251160']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5032488236', 'display_name': 'Mario Lebendiker', 'orcid': 'https://orcid.org/0000-0003-4821-1299'}, 'institutions': [{'id': 'https://openalex.org/I197251160', 'display_name': 'Hebrew University of Jerusalem', 'ror': 'https://ror.org/03qxff017', 'country_code': 'IL', 'type': 'education', 'lineage': ['https://openalex.org/I197251160']}], 'countries': ['IL'], 'is_corresponding': False, 'raw_author_name': 'Mario Lebendiker', 'raw_affiliation_strings': ['From the Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel'], 'affiliations': [{'raw_affiliation_string': 'From the Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel', 'institution_ids': ['https://openalex.org/I197251160']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5029719312', 'display_name': 'Shimon Schuldiner', 'orcid': 'https://orcid.org/0000-0002-4874-6237'}, 'institutions': [{'id': 'https://openalex.org/I197251160', 'display_name': 'Hebrew University of Jerusalem', 'ror': 'https://ror.org/03qxff017', 'country_code': 'IL', 'type': 'education', 'lineage': ['https://openalex.org/I197251160']}], 'countries': ['IL'], 'is_corresponding': False, 'raw_author_name': 'Shimon Schuldiner', 'raw_affiliation_strings': ['From the Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel'], 'affiliations': [{'raw_affiliation_string': 'From the Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, 91904 Jerusalem, Israel', 'institution_ids': ['https://openalex.org/I197251160']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'apc_paid': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'fwci': 2.462, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 120, 'citation_normalized_percentile': {'value': 0.906566, 'is_in_top_1_percent': False, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 96, 'max': 97}, 'biblio': {'volume': '271', 'issue': '49', 'first_page': '31044', 'last_page': '31048'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10147', 'display_name': 'Antibiotic Resistance in Bacteria', 'score': 0.9993, 'subfield': {'id': 'https://openalex.org/subfields/1313', 'display_name': 'Molecular Medicine'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10147', 'display_name': 'Antibiotic Resistance in Bacteria', 'score': 0.9993, 'subfield': {'id': 'https://openalex.org/subfields/1313', 'display_name': 'Molecular Medicine'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10120', 'display_name': 'Bacterial Genetics and Biotechnology', 'score': 0.9978, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10570', 'display_name': 'Drug Transport and Resistance Mechanisms', 'score': 0.9976, 'subfield': {'id': 'https://openalex.org/subfields/2730', 'display_name': 'Oncology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/dominance', 'display_name': 'Dominance (genetics)', 'score': 0.67853874}, {'id': 'https://openalex.org/keywords/antiporters', 'display_name': 'Antiporters', 'score': 0.47911167}], 'concepts': [{'id': 'https://openalex.org/C547475151', 'wikidata': 'https://www.wikidata.org/wiki/Q25419', 'display_name': 'Escherichia coli', 'level': 3, 'score': 0.7954953}, {'id': 'https://openalex.org/C57181701', 'wikidata': 'https://www.wikidata.org/wiki/Q2853339', 'display_name': 'Antiporter', 'level': 3, 'score': 0.71703005}, {'id': 'https://openalex.org/C151913843', 'wikidata': 'https://www.wikidata.org/wiki/Q3454555', 'display_name': 'Dominance (genetics)', 'level': 3, 'score': 0.67853874}, {'id': 'https://openalex.org/C133936738', 'wikidata': 'https://www.wikidata.org/wiki/Q643839', 'display_name': 'Multiple drug resistance', 'level': 3, 'score': 0.588431}, {'id': 'https://openalex.org/C2910532024', 'wikidata': 'https://www.wikidata.org/wiki/Q2853339', 'display_name': 'Antiporters', 'level': 4, 'score': 0.47911167}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.42773914}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.37447634}, {'id': 'https://openalex.org/C70721500', 'wikidata': 'https://www.wikidata.org/wiki/Q177005', 'display_name': 'Computational biology', 'level': 1, 'score': 0.34749126}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.2816707}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.120651305}, {'id': 'https://openalex.org/C501593827', 'wikidata': 'https://www.wikidata.org/wiki/Q12187', 'display_name': 'Antibiotics', 'level': 2, 'score': 0.07521677}, {'id': 'https://openalex.org/C41625074', 'wikidata': 'https://www.wikidata.org/wiki/Q176088', 'display_name': 'Membrane', 'level': 2, 'score': 0.07234475}], 'mesh': [{'descriptor_ui': 'D017920', 'descriptor_name': 'Antiporters', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D001426', 'descriptor_name': 'Bacterial Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D002352', 'descriptor_name': 'Carrier Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D008565', 'descriptor_name': 'Membrane Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D001426', 'descriptor_name': 'Bacterial Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001426', 'descriptor_name': 'Bacterial Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D002352', 'descriptor_name': 'Carrier Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002352', 'descriptor_name': 'Carrier Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D029968', 'descriptor_name': 'Escherichia coli Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008565', 'descriptor_name': 'Membrane Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008565', 'descriptor_name': 'Membrane Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D016297', 'descriptor_name': 'Mutagenesis, Site-Directed', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D010641', 'descriptor_name': 'Phenotype', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011487', 'descriptor_name': 'Protein Conformation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011510', 'descriptor_name': 'Proteolipids', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011510', 'descriptor_name': 'Proteolipids', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.271.49.31044', 'pdf_url': 'http://www.jbc.org/article/S0021925819789510/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/8940098', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.271.49.31044', 'pdf_url': 'http://www.jbc.org/article/S0021925819789510/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'score': 0.48, 'id': 'https://metadata.un.org/sdg/6', 'display_name': 'Clean water and sanitation'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 27, 'referenced_works': ['https://openalex.org/W1543647466', 'https://openalex.org/W1970533648', 'https://openalex.org/W1970884855', 'https://openalex.org/W1975085160', 'https://openalex.org/W1984899973', 'https://openalex.org/W2006643836', 'https://openalex.org/W2028577558', 'https://openalex.org/W2028622989', 'https://openalex.org/W2042100725', 'https://openalex.org/W2045885130', 'https://openalex.org/W2053690386', 'https://openalex.org/W2060913542', 'https://openalex.org/W2068518785', 'https://openalex.org/W2071416115', 'https://openalex.org/W2076685916', 'https://openalex.org/W2087941147', 'https://openalex.org/W2088734315', 'https://openalex.org/W2091280104', 'https://openalex.org/W2102131116', 'https://openalex.org/W2106386139', 'https://openalex.org/W2129108032', 'https://openalex.org/W2153360089', 'https://openalex.org/W2157375015', 'https://openalex.org/W2162097287', 'https://openalex.org/W2167131009', 'https://openalex.org/W2195565602', 'https://openalex.org/W2313380666'], 'related_works': ['https://openalex.org/W4382369436', 'https://openalex.org/W3110024609', 'https://openalex.org/W2272807793', 'https://openalex.org/W2270997957', 'https://openalex.org/W2148522077', 'https://openalex.org/W2146546082', 'https://openalex.org/W1997387703', 'https://openalex.org/W1997200285', 'https://openalex.org/W1992734373', 'https://openalex.org/W1987091393'], 'abstract_inverted_index': {'EmrE,': [0, 250], 'the': [1, 43, 77, 80, 88, 112, 116, 124, 127, 143, 168, 171, 189, 196, 209, 213, 225, 236, 242, 251, 293, 327, 330, 338, 362, 366, 374, 377, 393, 418, 421, 439, 446, 459, 463, 475, 486, 492], 'smallest': [2, 252], 'known': [3, 253], 'ion-coupled': [4, 248, 254, 498], 'transporter,': [5, 255], 'is': [6, 20, 38, 186, 227, 256, 270, 288, 436, 477], 'an': [7, 247, 257, 497], 'Escherichia': [8, 258], 'coli': [9, 259], '12-kDa': [10, 260], 'protein': [11, 82, 118, 261, 332, 368], '80%': [12, 262], 'helical': [13, 263], 'and': [14, 70, 156, 264, 320, 406], 'soluble': [15, 265], 'in': [16, 120, 123, 140, 154, 173, 194, 238, 266, 370, 373, 390, 404, 423, 444, 488], 'organic': [17, 151, 267, 401], 'solvents.': [18, 268], 'EmrE': [19, 269], 'a': [21, 62, 103, 121, 174, 271, 312, 353, 371, 424], 'polyspecific': [22, 272], 'antiporter': [23, 273], 'that': [24, 183, 224, 274, 433, 474], 'exchanges': [25, 275], 'hydrogen': [26, 276], 'ions': [27, 277], 'with': [28, 115, 150, 163, 278, 365, 400, 413], 'aromatic': [29, 279], 'toxic': [30, 280], 'cations': [31, 281], 'such': [32, 282], 'as': [33, 283], 'methyl': [34, 98, 284, 348], 'viologen.': [35, 285], 'Since': [36, 286], 'it': [37, 50, 287, 300], 'many': [39, 289], 'times': [40, 290], 'smaller': [41, 291], 'than': [42, 292], 'classical': [44, 294], 'consensus': [45, 295], '12': [46, 296], 'transmembrane': [47, 297], 'segments': [48, 298], 'transporters,': [49, 299], 'was': [51, 301], 'particularly': [52, 302], 'interesting': [53, 303], 'to': [54, 72, 87, 97, 130, 133, 158, 178, 188, 304, 322, 337, 347, 380, 383, 408, 428, 438], 'determine': [55, 305], 'its': [56, 306], 'oligomeric': [57, 244, 307, 494], 'state.': [58, 308], 'For': [59, 309], 'this': [60, 184, 310, 434], 'purpose,': [61, 311], 'series': [63, 104, 313, 354], 'of': [64, 79, 105, 109, 111, 126, 170, 191, 198, 208, 229, 241, 246, 314, 329, 355, 359, 361, 376, 420, 441, 448, 458, 479, 491, 496], 'nonfunctional': [65, 113, 200, 315, 363, 450], 'mutants': [66, 92, 114, 144, 316, 342, 364, 394], 'has': [67, 317], 'been': [68, 146, 318, 396], 'generated': [69, 319], 'characterized': [71, 321], 'test': [73, 323], 'their': [74, 324], 'effect': [75, 325], 'on': [76, 212, 326, 462], 'activity': [78, 169, 328, 419], 'wild-type': [81, 117, 164, 331, 367, 414], 'upon': [83, 333], 'mixing.': [84, 334], 'As': [85, 335], 'opposed': [86, 336], 'wild': [89, 339], 'type,': [90, 340], 'these': [91, 341], 'do': [93, 216, 343, 466], 'not': [94, 217, 344, 467], 'confer': [95, 131, 345, 381], 'resistance': [96, 132, 346, 382], 'viologen,': [99, 349], 'ethidium': [100, 350], 'bromide,': [101, 351], 'or': [102, 352], 'other': [106, 356], 'toxicants.': [107, 135, 357, 385], 'Co-expression': [108, 358], 'each': [110, 360], 'results': [119, 210, 233, 369, 460, 483], 'reduction': [122, 372], 'ability': [125, 375], 'functional': [128, 243, 378, 493], 'transporter': [129, 379], 'several': [134, 384], 'To': [136, 386], 'perform': [137, 387], 'mixing': [138, 388], 'experiments': [139, 389], 'vitro,': [141, 391], 'all': [142, 392], 'have': [145, 395], 'purified': [147, 397], 'by': [148, 398], 'extraction': [149, 399], 'solvents,': [152, 402], 'reconstituted': [153, 403], 'proteoliposomes,': [155, 405], 'found': [157, 407], 'be': [159, 409], 'inactive.': [160, 410], 'When': [161, 411], 'co-reconstituted': [162, 412], 'protein,': [165, 415], 'they': [166, 416], 'inhibit': [167, 417], 'latter': [172, 214, 422, 464], 'dose-dependent': [175, 425], 'form': [176, 426], 'up': [177, 427], 'full': [179, 203, 429, 453], 'inhibition.': [180, 430], 'We': [181, 431], 'assume': [182, 432], 'inhibition': [185, 435], 'due': [187, 437], 'formation': [190, 440], 'mixed': [192, 442], 'oligomers': [193, 443], 'which': [195, 445], 'presence': [197, 447], 'one': [199, 449], 'subunit': [201, 451], 'causes': [202, 452], 'inactivation.': [204, 454], 'A': [205, 455], 'binomial': [206, 456], 'analysis': [207, 457], 'based': [211, 461], 'assumptions': [215, 465], 'provide': [218, 235, 468, 485], 'statistically': [219, 469], 'significant': [220, 470], 'answers': [221, 471], 'but': [222, 472], 'suggests': [223, 473], 'oligomer': [226, 476], 'composed': [228, 478], 'three': [230, 480], 'subunits.': [231, 481], 'The': [232, 482], 'described': [234, 484], 'first': [237, 487], 'vitro': [239, 489], 'demonstration': [240, 490], 'structure': [245, 495], 'transporter.': [249, 499]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2003343658', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2023, 'cited_by_count': 2}, {'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 2}, {'year': 2018, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 2}, {'year': 2016, 'cited_by_count': 4}, {'year': 2015, 'cited_by_count': 1}, {'year': 2014, 'cited_by_count': 7}, {'year': 2013, 'cited_by_count': 3}, {'year': 2012, 'cited_by_count': 7}], 'updated_date': '2024-12-15T03:39:09.289477', 'created_date': '2016-06-24'}