Title: The proteome and secretome of human arterial smooth muscle cells
Abstract: PROTEOMICSVolume 5, Issue 2 p. 585-596 Regular Article The proteome and secretome of human arterial smooth muscle cells Annabelle Dupont, Annabelle Dupont INSERM, U508, Institut Pasteur de Lille, Lille, FranceSearch for more papers by this authorDelphine Corseaux, Delphine Corseaux INSERM, ER919, University of Lille 2, Lille, FranceSearch for more papers by this authorOlivia Dekeyzer, Olivia Dekeyzer INSERM, U508, Institut Pasteur de Lille, Lille, FranceSearch for more papers by this authorHervé Drobecq, Hervé Drobecq INSERM, UMR 8525, Institut Pasteur de Lille, Lille, FranceSearch for more papers by this authorAnne-Laure Guihot, Anne-Laure Guihot INSERM, U508, Institut Pasteur de Lille, Lille, FranceSearch for more papers by this authorSophie Susen, Sophie Susen INSERM, ER919, University of Lille 2, Lille, FranceSearch for more papers by this authorAndré Vincentelli, André Vincentelli INSERM, ER919, University of Lille 2, Lille, FranceSearch for more papers by this authorPhilippe Amouyel, Philippe Amouyel INSERM, U508, Institut Pasteur de Lille, Lille, FranceSearch for more papers by this authorBrigitte Jude, Brigitte Jude INSERM, ER919, University of Lille 2, Lille, FranceSearch for more papers by this authorFlorence Pinet, Corresponding Author Florence Pinet [email protected] INSERM, U508, Institut Pasteur de Lille, Lille, FranceINSERM U508, Institut Pasteur de Lille, 1 rue du professeur Calmette, 59019 Lille, France Fax: +33-3-20-87-78-94===Search for more papers by this author Annabelle Dupont, Annabelle Dupont INSERM, U508, Institut Pasteur de Lille, Lille, FranceSearch for more papers by this authorDelphine Corseaux, Delphine Corseaux INSERM, ER919, University of Lille 2, Lille, FranceSearch for more papers by this authorOlivia Dekeyzer, Olivia Dekeyzer INSERM, U508, Institut Pasteur de Lille, Lille, FranceSearch for more papers by this authorHervé Drobecq, Hervé Drobecq INSERM, UMR 8525, Institut Pasteur de Lille, Lille, FranceSearch for more papers by this authorAnne-Laure Guihot, Anne-Laure Guihot INSERM, U508, Institut Pasteur de Lille, Lille, FranceSearch for more papers by this authorSophie Susen, Sophie Susen INSERM, ER919, University of Lille 2, Lille, FranceSearch for more papers by this authorAndré Vincentelli, André Vincentelli INSERM, ER919, University of Lille 2, Lille, FranceSearch for more papers by this authorPhilippe Amouyel, Philippe Amouyel INSERM, U508, Institut Pasteur de Lille, Lille, FranceSearch for more papers by this authorBrigitte Jude, Brigitte Jude INSERM, ER919, University of Lille 2, Lille, FranceSearch for more papers by this authorFlorence Pinet, Corresponding Author Florence Pinet [email protected] INSERM, U508, Institut Pasteur de Lille, Lille, FranceINSERM U508, Institut Pasteur de Lille, 1 rue du professeur Calmette, 59019 Lille, France Fax: +33-3-20-87-78-94===Search for more papers by this author First published: 08 February 2005 https://doi.org/10.1002/pmic.200400965Citations: 75AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Smooth muscle cells (SMCs) play a crucial role in cardiovascular disorders. A differential proteomic approach should help to elucidate SMC dysfunctions involved in these diseases. With this goal in mind, we plotted the first 2-dimensional (2-D) maps of the proteome and secretome of human arterial smooth muscle cell (ASMC). Intracellular and secreted proteins were extracted from a primary culture of SMCs obtained from patients undergoing coronary artery bypass surgery (n = 11) and separated by 2-dimensional gel electrophoresis. Silver-stained gels were analyzed using Progenesis software. A high level of between-gel reproducibility was obtained, allowing us to generate two protein patterns specific to the ASMC proteome and secretome, respectively. A total of 121 and 40 distinct intracellular and secreted polypeptide spots, corresponding to 83 and 18 different proteins, respectively, were identified by matrix-assisted laser desorption/ionization mass spectrometry. The 2-D reference maps and database resulting from this study confirm that SMCs are involved in a wide range of biological functions. They could constitute a useful tool for a wide range of investigators involved in vascular biology, allowing them to investigate SMC protein changes associated with cardiovascular disorders or environmental stimuli. Citing Literature Volume5, Issue2No. 2 February 2005Pages 585-596 RelatedInformation
Publication Year: 2005
Publication Date: 2005-02-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 91
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