Title: A1H NMR Spectroscopic Approach to the Unambiguous Determination of Glycosyl Linkage Positions in Oligosaccharides
Abstract: A sensitive (<1 mg) and nondestructive method was devised for the unambiguous determination of the glycosyl linkage positions in oligosaccharides using1H NMR spectroscopy. The technique is based on the absence of a hydroxyl group on the carbon atom that participates in the glycosidic linkage. The "missing" hydroxyl group is identified by recording1H NMR spectra of the oligosaccharide in H2O and D2O. The method is illustrated by determining the glycosyl linkage positions of maltose, Glcα1-4Glc, and a branched mannopentaose, Manα1-6(Manα1-3)Manα1-6(Manα1-3)Man.
Publication Year: 1998
Publication Date: 1998-02-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 17
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