Multiple Molecular Determinants in the Carboxyl Terminus Regulate Dopamine Transporter Export from Endoplasmic Reticulum

Name: Work Video:
Duration: 3 min 30 s
Manuel Miranda; Tatiana Sorkina; Tom N. Grammatopoulos; W. Michael Zawada; Alexander Sorkin
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2603, 2669, 2694, 2790, 2852, 3036, 3063, 3670, 3887, 3991, 4004, 4055, 4420], 'plasma': [1, 62, 109, 236, 297, 344, 542, 683, 1177, 1629, 3491, 3591, 3632, 3876, 3914, 3963, 4104, 4136, 4158, 4196, 4202, 4264, 4343, 4424], 'membrane': [2, 110, 237, 345, 543, 684, 1000, 1285, 1569, 1617, 1630, 3592, 3877, 3915, 3922, 4105, 4137, 4159, 4203, 4425], 'dopamine': [3, 17, 238, 252, 516, 534, 544, 552, 616, 822, 3833], 'transporter': [4, 55, 239, 290, 545, 1173, 1289, 1441, 3611, 3949, 3957], '(DAT)': [5, 240], 'has': [6, 241, 1186, 1427, 3078, 3714], 'an': [7, 242, 1585, 4397], 'essential': [8, 227, 243, 462, 1433, 1619, 3404], 'role': [9, 44, 244, 279, 475, 4183], 'in': [10, 51, 84, 116, 156, 245, 286, 319, 351, 391, 476, 723, 818, 825, 830, 838, 841, 854, 861, 864, 869, 925, 941, 979, 1007, 1016, 1130, 1159, 1424, 1510, 1576, 1599, 1609, 1631, 1702, 1774, 1907, 1986, 1999, 2085, 2109, 2199, 2258, 2356, 2384, 2407, 2446, 2512, 2539, 2551, 2638, 2691, 2838, 2857, 2866, 2925, 3040, 3311, 3321, 3414, 3440, 3694, 3709, 3836, 3852, 3855, 3881, 3894, 3918, 3924, 3939, 3943, 3988, 4066, 4113, 4156, 4189, 4233, 4275, 4295, 4302, 4312, 4338, 4353, 4404], 'terminating': [11, 246], 'dopaminergic': [12, 247, 538], 'neurotransmission': [13, 248, 471], 'by': [14, 249, 513, 530, 540, 727, 968, 1707, 1789, 1800, 1803, 1826, 1830, 1834, 1888, 1903, 1924, 1938, 1967, 1981, 2011, 2195, 2215, 2362, 2515, 2607, 2634, 2689, 2701, 2725, 2736, 2786, 2910, 2978, 3208, 3468, 3473, 3640, 3680, 3884, 3903, 4160, 4173], 'reuptake': [15, 250, 532], 'of': [16, 25, 35, 45, 49, 53, 78, 97, 105, 112, 114, 124, 132, 140, 145, 152, 171, 212, 215, 232, 251, 260, 270, 280, 284, 288, 313, 332, 340, 347, 349, 359, 367, 375, 380, 387, 406, 447, 450, 467, 478, 481, 515, 533, 681, 821, 850, 867, 923, 930, 949, 959, 987, 995, 999, 1020, 1134, 1149, 1157, 1168, 1182, 1196, 1260, 1265, 1273, 1283, 1287, 1422, 1439, 1500, 1506, 1553, 1560, 1566, 1571, 1589, 1593, 1622, 1634, 1643, 1653, 1663, 1885, 1898, 1928, 1954, 1960, 1974, 1989, 2022, 2062, 2202, 2220, 2754, 2934, 2951, 2994, 3002, 3009, 3065, 3150, 3198, 3253, 3263, 3270, 3316, 3331, 3347, 3371, 3382, 3471, 3572, 3609, 3619, 3628, 3635, 3673, 3691, 3859, 3875, 3889, 3928, 3935, 3946, 3973, 3981, 4018, 4084, 4107, 4115, 4132, 4149, 4169, 4184, 4192, 4205, 4217, 4225, 4245, 4255, 4259, 4270, 4279, 4329, 4334, 4341, 4348, 4355, 4441, 4445, 4455, 4464], 'into': [18, 253, 536, 1003, 1854, 1913, 1919, 2185], 'the': [19, 23, 28, 43, 46, 54, 57, 61, 76, 85, 103, 108, 117, 130, 135, 141, 162, 185, 193, 203, 206, 213, 216, 222, 229, 233, 254, 258, 263, 278, 281, 289, 292, 296, 311, 320, 338, 343, 352, 365, 370, 376, 397, 420, 428, 438, 441, 448, 451, 457, 464, 468, 518, 531, 537, 541, 679, 689, 724, 812, 858, 870, 920, 935, 942, 950, 953, 962, 985, 990, 996, 1008, 1017, 1023, 1171, 1176, 1261, 1270, 1281, 1288, 1354, 1361, 1425, 1435, 1440, 1503, 1567, 1572, 1577, 1580, 1590, 1602, 1610, 1616, 1625, 1628, 1654, 1664, 1721, 1736, 1761, 1775, 1809, 1843, 1848, 1877, 1886, 1899, 1904, 1914, 1920, 1932, 1946, 1955, 1961, 1971, 1990, 2019, 2059, 2063, 2144, 2154, 2171, 2189, 2197, 2200, 2270, 2294, 2305, 2425, 2481, 2524, 2617, 2636, 2652, 2707, 2738, 2751, 2755, 2759, 2772, 2921, 2936, 3003, 3072, 3118, 3144, 3151, 3196, 3203, 3250, 3256, 3261, 3264, 3277, 3332, 3350, 3360, 3367, 3372, 3380, 3385, 3416, 3425, 3450, 3460, 3469, 3483, 3490, 3493, 3506, 3573, 3579, 3590, 3600, 3610, 3616, 3620, 3631, 3646, 3656, 3659, 3689, 3695, 3827, 3840, 3853, 3913, 3925, 3940, 3953, 3959, 3962, 3997, 4067, 4072, 4079, 4130, 4135, 4142, 4146, 4157, 4182, 4190, 4195, 4201, 4243, 4256, 4263, 4298, 4317, 4321, 4330, 4339, 4342, 4356, 4405, 4417, 4439, 4442, 4452, 4465], 'presynaptic': [20, 255, 519, 954], 'neurons.': [21, 256, 1646], 'Therefore,': [22, 257], 'amount': [24, 259, 958, 3197, 4269], 'DAT': [26, 36, 50, 89, 106, 115, 133, 153, 176, 217, 261, 271, 285, 324, 341, 350, 368, 388, 411, 452, 676, 716, 815, 851, 924, 960, 988, 1158, 1184, 1197, 1267, 1352, 1423, 1507, 1574, 1623, 1747, 1782, 1901, 2442, 2723, 2776, 2783, 2795, 3028, 3254, 3317, 3348, 3383, 3406, 3412, 3448, 3472, 3587, 3629, 3636, 3653, 3676, 3692, 3816, 3822, 3843, 4060, 4133, 4170, 4193, 4315], 'at': [27, 262, 746, 952, 961, 1760, 1808, 1876, 2375, 2411, 2600, 2612, 2641, 2666, 2828, 2849, 2873, 2912, 2989, 3215, 3359, 3489, 3846], 'cell': [29, 65, 69, 165, 186, 264, 300, 304, 400, 421, 826, 963, 1161, 1193, 1362, 2133, 2191, 2209, 3204, 3278, 3351, 3361, 3386, 3657, 3683, 3828, 3905, 4073, 4210], 'surface': [30, 70, 138, 265, 305, 373, 848, 964, 1194, 1363, 3205, 3279, 3362, 3387, 3447, 3477, 3829, 4211, 4314], 'is': [31, 266, 511, 717, 816, 852, 965, 1028, 1139, 1357, 1556, 3603, 3823, 3844, 4015, 4290, 4402], 'a': [32, 267, 473, 479, 718, 1131, 1166, 1558, 1729, 1755, 1778, 1805, 1835, 1983, 2000, 2368, 2408, 2516, 2835, 2952, 2958, 2971, 3000, 3041, 3047, 3455, 3856, 3944, 3971, 4016, 4082, 4101, 4408], 'critical': [33, 268, 474, 3624], 'determinant': [34, 269], 'function.': [37, 272], 'In': [38, 168, 273, 403, 811, 1350, 1579, 1993, 2647, 2769, 3443, 3865, 4140, 4305, 4468], 'this': [39, 274, 980, 3994, 4012, 4288], 'study,': [40, 275], 'we': [41, 276, 982, 1583, 3327, 4208], 'examined': [42, 277], 'carboxyl': [47, 86, 218, 282, 321, 453, 734, 1180, 1611, 3374, 3621], 'terminus': [48, 87, 219, 283, 322, 454, 1181, 1356, 1612, 2021, 3375, 3622], 'trafficking': [52, 287, 973, 1174, 1286, 3588], 'through': [56, 291, 3059], 'biosynthetic': [58, 293], 'pathway': [59, 294], 'to': [60, 74, 107, 126, 184, 192, 221, 295, 309, 342, 361, 419, 427, 456, 678, 738, 938, 1038, 1175, 1189, 1360, 1431, 1601, 1615, 1627, 1735, 1764, 1812, 1880, 1931, 2035, 2046, 2058, 2104, 2127, 2130, 2138, 2274, 2658, 2711, 2722, 2749, 3070, 3255, 3276, 3349, 3384, 3445, 3512, 3578, 3589, 3630, 3644, 3655, 3666, 3718, 3826, 3912, 3961, 3993, 4134, 4194, 4262, 4396, 4416], 'membrane.': [63, 298, 1178, 2713, 3633, 3964, 4197, 4265, 4344], 'Live': [64, 299, 3017], 'fluorescence': [66, 301, 566, 630, 2937, 3313, 3684, 4177, 4299, 4333], 'microscopy': [67, 302, 3314, 3685, 4178, 4300, 4319], 'and': [68, 81, 99, 137, 161, 179, 188, 303, 316, 334, 372, 396, 414, 423, 490, 522, 525, 558, 561, 622, 625, 694, 733, 828, 833, 837, 843, 863, 919, 945, 947, 1032, 1137, 1640, 1648, 1660, 1728, 1751, 1771, 1785, 1802, 1815, 1916, 1941, 1943, 1957, 1970, 2003, 2048, 2077, 2094, 2100, 2117, 2141, 2165, 2188, 2265, 2277, 2297, 2382, 2414, 2428, 2439, 2449, 2493, 2498, 2541, 2616, 2632, 2686, 2697, 2706, 2732, 2758, 2765, 2777, 2782, 2796, 2831, 2844, 2882, 2904, 2970, 3020, 3044, 3053, 3076, 3111, 3117, 3126, 3147, 3195, 3419, 3424, 3431, 3459, 3612, 3830, 3850, 3862, 3871, 3878, 3891, 3921, 3955, 3977, 4001, 4058, 4074, 4109, 4152, 4187, 4248, 4407, 4435, 4447], 'biotinylation': [71, 306, 3478, 3513, 4212, 4307], 'were': [72, 182, 307, 417, 1670, 1718, 1787, 2009, 2050, 2083, 2107, 2136, 2156, 2166, 2183, 2193, 2256, 2272, 2290, 2320, 2354, 2372, 2400, 2421, 2444, 2483, 2526, 2548, 2605, 2620, 2655, 2671, 2699, 2709, 2734, 2747, 2761, 2780, 2792, 2806, 2863, 2908, 2923, 2987, 3030, 3038, 3057, 3114, 3141, 3190, 3422, 3437, 3452, 3463, 3502, 3707, 3808, 3996, 4062, 4251, 4351, 4450, 4459], 'used': [73, 308, 549, 613, 2167, 2429, 3069], 'study': [75, 310, 981, 1582], 'effects': [77, 312], 'systematic': [79, 314, 3667], 'deletions': [80, 315], 'alanine': [82, 95, 127, 317, 330, 362, 3979], 'substitutions': [83, 96, 318, 331, 1717, 3980], 'on': [88, 149, 323, 384, 984, 2170, 2402, 2503, 2533, 2703, 2803, 3032, 3046, 3192, 3202, 3958], 'localization.': [90, 325], 'It': [91, 326], 'was': [92, 154, 327, 389, 1704, 1758, 1824, 1852, 1911, 1922, 1936, 1979, 1997, 2004, 2024, 2037, 2066, 2125, 2212, 2716, 2784, 2855, 2947, 2997, 3012, 3122, 3157, 3206, 3428, 3486, 3496, 3664, 3678, 3698, 3897, 3910, 3937, 3968, 4007, 4095, 4111, 4153, 4221, 4239, 4273, 4309, 4428], 'found': [93, 328, 817], 'that': [94, 329, 1141, 1146, 1276, 1598, 3329, 3394, 3402, 3481, 3599, 3613, 4119, 4287, 4401, 4427, 4463], 'Lys-590': [98, 333], 'Asp-600': [100, 335, 4188], 'significantly': [101, 336, 4460], 'delayed': [102, 337, 3357], 'delivery': [104, 339, 946, 3381, 3627, 4131, 4258], 'because': [111, 346, 3819, 4011, 4320], 'retention': [113, 348, 948, 1552], 'endoplasmic': [118, 353, 578, 642], 'reticulum': [119, 354], '(ER).': [120, 355], 'Most': [121, 356], 'surprising,': [122, 357], 'mutation': [123, 358, 4006], 'Gly-585': [125, 360], 'completely': [128, 363, 3344, 4124], 'blocked': [129, 364, 3379], 'exit': [131, 366, 1513], 'from': [134, 369, 517, 934, 989, 1624, 2026, 2038, 2051, 2068, 2314, 2651, 2742, 4223], 'ER': [136, 150, 230, 371, 385, 465, 1009, 1040, 1152, 1426, 1437, 1512, 1551, 1626, 3607, 3854, 4088, 4150], 'expression': [139, 374, 849, 1162, 1195, 3358, 3813, 4204], 'transporter.': [142, 234, 377, 469], 'effect': [144, 379], 'these': [146, 381, 972, 1554, 3354, 3410, 3837, 4120, 4260], 'three': [147, 382, 748, 1263, 1605, 1632, 1791, 3266], 'mutations': [148, 383, 1501, 2008, 3706, 4121], 'export': [151, 231, 386, 466, 929, 1041, 1153, 1438, 3608], 'demonstrated': [155, 390, 1188, 3480], 'porcine': [157, 392, 574, 638], 'aortic': [158, 393, 575, 639, 2079], 'endothelial': [159, 394, 2080], 'cells': [160, 395, 1639, 2082, 2102, 2155, 2198, 2255, 2271, 2289, 2420, 2482, 2525, 2547, 2773, 2801, 3023, 3183, 3210, 3451, 3807, 3868, 3883, 3896, 3930], 'immortalized': [163, 398, 1637, 2207], 'neuronal': [164, 399, 1638, 2208], 'line': [166, 401, 2210], '1RB3AN27.': [167, 402], 'primary': [169, 404, 1641, 2880, 2903], 'cultures': [170, 405, 1642, 2312, 2353], 'rat': [172, 407, 2016, 2317, 2720, 2788], 'embryonic': [173, 408, 1644, 2316], 'midbrain': [174, 409, 1645], 'neurons,': [175, 410, 823], 'G585A,': [177, 412, 3998, 4056], 'K590A,': [178, 413, 3999, 4057], 'D600A': [180, 415, 4059, 4110, 4249], 'mutants': [181, 416, 1555, 1783, 3319, 3501, 3677, 3693, 3893, 3974, 4061, 4250, 4261], 'restricted': [183, 418], 'soma': [187, 422], 'did': [189, 424, 3342, 3584, 4122], 'not': [190, 425, 1011, 1998, 2005, 3343, 3364, 3389, 3585, 3594, 3604, 4008, 4123, 4154, 4325, 4429], 'traffic': [191, 426], 'dendrites': [194, 429, 829, 868], 'or': [195, 430, 1724, 1797, 2173, 2180, 2308, 2433, 2894, 3187, 3324, 3336], 'axonal': [196, 431, 839, 859], 'processes.': [197, 432], 'These': [198, 433, 1123, 2254, 3391, 3596, 3948, 3984], 'data': [199, 434, 3985], 'are': [200, 226, 435, 461, 527, 736, 975, 1010, 1147, 1618, 3132, 3396, 3403, 3623, 3869, 3986], 'consistent': [201, 436, 4086], 'with': [202, 437, 1269, 1278, 1945, 2143, 2262, 2280, 2293, 2299, 2367, 2424, 2485, 2505, 2519, 2529, 2535, 2543, 2594, 2622, 2629, 2660, 2674, 2678, 2718, 2729, 2794, 2808, 2814, 2859, 2879, 2888, 2892, 2957, 3211, 3454, 3916, 3975, 4078, 4087, 4228, 4297], 'model': [204, 439, 1160], 'whereby': [205, 440], 'local': [207, 442], 'conformation': [208, 443], 'and/or': [209, 444, 1564], 'intramolecular': [210, 445], 'interactions': [211, 446], 'sequences': [214, 449, 3614], 'proximal': [220, 455, 1614], 'last': [223, 458, 1262, 1665, 3265, 3333, 3417, 3574], 'transmembrane': [224, 459, 1021, 1135, 1504, 1958], 'domain': [225, 460, 1272, 1935, 2061, 4022], 'for': [228, 463, 1170, 1192, 1434, 1620, 2168, 2285, 2304, 2379, 2416, 2430, 2500, 2609, 2644, 2663, 2825, 2876, 2885, 2916, 3218, 3249, 3273, 3405, 3606, 3625, 3652, 3815, 3970], 'Dopaminergic': [470], 'plays': [472], 'modulation': [477], 'variety': [480], 'central': [482], 'nervous': [483], 'system': [484, 3814], 'functions,': [485], 'including': [486, 824, 1636], 'locomotor': [487], 'activity,': [488], 'cognition,': [489], 'reward': [491], '(1Cohen': [492], 'J.D.': [493, 3779], 'Braver': [494], 'T.S.': [495, 3789], 'Brown': [496], 'J.W.': [497], 'Curr.': [498], 'Opin.': [499], 'Neurobiol.': [500], '2002;': [501, 805, 3798], '12:': [502], '223-229Crossref': [503], 'PubMed': [504, 711, 772, 794, 808, 893, 913, 1050, 1070, 1099, 1118, 1223, 1253, 1315, 1345, 1389, 1415, 1467, 1493, 1525, 1545, 1694, 1871, 2249, 2347, 2475, 3103, 3177, 3239, 3305, 3539, 3565, 3743, 3763, 3801, 4050, 4383], 'Scopus': [505, 712, 773, 795, 1051, 1071, 1100, 1119, 1224, 1254, 1316, 1346, 1390, 1416, 1468, 1494, 1526, 1546, 1695, 1872, 2250, 2348, 2476, 3104, 3178, 3240, 3306, 3540, 3566, 3744, 3764, 3802, 4051, 4384], '(297)': [506], 'Google': [507, 714, 775, 797, 809, 894, 914, 1053, 1073, 1102, 1121, 1226, 1256, 1318, 1348, 1392, 1418, 1470, 1496, 1528, 1548, 1697, 1874, 2252, 2350, 2478, 3106, 3180, 3242, 3308, 3542, 3568, 3746, 3766, 3804, 4053, 4386], 'Scholar).': [508, 715, 810, 915, 1122, 1257, 1349, 1419, 1497, 1549, 1698, 2253, 2351, 2479, 3107, 3181, 3243, 3309, 3569, 3805, 4054, 4387], 'Dopamine': [509], 'signaling': [510], 'initiated': [512], 'release': [514], 'neuron': [520], 'cell,': [521, 3696], 'its': [523, 3274, 3892], 'duration': [524], 'intensity': [526], 'regulated': [528, 967], 'primarily': [529], 'back': [535], 'neurons': [539, 926], '(DAT).': [546], '1The': [547], 'abbreviations': [548, 612], 'are:': [550, 614], 'DAT,': [551, 615], 'transporter;': [553, 593, 596, 617, 657, 660], 'hDAT,': [554, 618], 'human': [555, 619, 1183, 1266, 1900], 'DAT;': [556, 620], 'YFP': [557, 621, 3135], 'CFP,': [559, 623, 3110], 'yellow': [560, 624], 'cyan': [562, 626], 'fluorescent': [563, 589, 627, 653, 3906], 'protein;': [564, 590, 628, 654], 'FRET,': [565, 629], 'resonance': [567, 631], 'energy': [568, 632], 'transfer;': [569, 633], 'FRETC,': [570, 634], 'corrected': [571, 635, 3119], 'FRET;': [572, 636], 'PAE,': [573, 637], 'endothelial;': [576, 640], 'ER,': [577, 641, 936, 4406], 'reticulum;': [579, 643], 'EGF,': [580, 644], 'epidermal': [581, 645], 'growth': [582, 646], 'factor;': [583, 647], 'EGFR,': [584, 648], 'EGF': [585, 649, 1844, 1962, 2064, 3154, 3199], 'receptor;': [586, 650], 'GFP,': [587, 651], 'green': [588, 652], 'NET,': [591, 655], 'norepinephrine': [592, 656], 'SERT,': [594, 658], 'serotonin': [595, 659, 691], 'FBS,': [597, 661, 2389], 'fetal': [598, 662, 2090], 'bovine': [599, 663, 2091, 2846, 2870], 'serum;': [600, 664], 'PBS,': [601, 665, 2513, 2531, 2540], 'phosphate-buffered': [602, 666, 2487], 'saline;': [603, 667], 'CMF,': [604, 668], 'Ca2+,': [605, 669, 2809], 'Mg2+-free;': [606, 670], 'PDI,': [607, 671], 'protein-disulfide': [608, 672], 'isomerase;': [609, 673], 'NHS,': [610, 674], 'N-hydroxysuccinimide.1The': [611], 'N-hydroxysuccinimide.': [675], 'belongs': [677], 'family': [680], 'Na+/Cl--dependent': [682], 'neurotransmitter': [685], 'transporters,': [686], 'which': [687, 1908, 2949, 3415], 'includes': [688], 'norepinephrine,': [690], '(SERT),': [692], 'glycine,': [693], 'γ-aminobutyric': [695], 'acid': [696, 720, 1658, 1668, 1716, 1766, 1882, 3649], 'transporters': [697, 933, 951], '(2Giros': [698], 'B.': [699], 'Caron': [700, 1212, 1240, 1304, 1332, 1376, 1454, 3294, 3526, 4039], 'M.G.': [701, 1213, 1241, 1305, 1333, 1377, 1455, 3295, 3527, 4040], 'Trends': [702, 1061], 'Pharmacol.': [703, 1521, 1541], 'Sci.': [704, 2242], '1993;': [705], '14:': [706], '43-49Abstract': [707], 'Full': [708, 789, 791, 1067, 1218, 1220, 1248, 1250, 1310, 1312, 1340, 1342, 1384, 1386, 1410, 1412, 1462, 1464, 1488, 1490, 1689, 1691, 1866, 1868, 2470, 2472, 3098, 3100, 3172, 3174, 3234, 3236, 3300, 3302, 3534, 3536, 3560, 3562, 3738, 3740, 3758, 3760, 4045, 4047, 4378, 4380], 'Text': [709, 790, 792, 1068, 1219, 1221, 1249, 1251, 1311, 1313, 1341, 1343, 1385, 1387, 1411, 1413, 1463, 1465, 1489, 1491, 1690, 1692, 1867, 1869, 2471, 2473, 3099, 3101, 3173, 3175, 3235, 3237, 3301, 3303, 3535, 3537, 3561, 3563, 3739, 3741, 3759, 3761, 4046, 4048, 4379, 4381], 'PDF': [710, 793, 1069, 1222, 1252, 1314, 1344, 1388, 1414, 1466, 1492, 1693, 1870, 2474, 3102, 3176, 3238, 3304, 3538, 3564, 3742, 3762, 4049, 4382], '(481)': [713], '620-amino': [719], 'polypeptide': [721], 'embedded': [722], 'lipid': [725], 'bilayer': [726], '12': [728], 'hydrophobic': [729], 'segments.': [730], 'Both': [731, 2902, 3500], 'amino': [732, 1030, 1355, 1657, 1667, 1715, 1765, 1881, 2020, 3268, 3338, 3648], 'termini': [735, 840], 'predicted': [737], 'be': [739, 1190, 1432, 3719], 'intracellular.': [740], 'second': [742, 2517], 'extracellular': [743, 1956, 2060], 'loop': [744], 'contains': [745], 'least': [747], 'consensus': [749], 'N-glycosylation': [750], 'sites': [751, 3201], '(3Shimada': [752], 'S.': [753, 755, 780, 1082, 1397, 1475, 1676, 2244, 2457, 3085, 3547, 3725, 4365], 'Kitayama': [754], 'Lin': [756, 3792], 'C.L.': [757], 'Patel': [758], 'A.': [759, 800, 879, 1084, 1231, 1237, 1323, 1329, 1367, 1373, 1403, 1445, 1451, 1481, 1682, 1859, 2245, 2463, 3091, 3165, 3227, 3517, 3523, 3553, 3731, 3795, 4371], 'Nanthakumar': [760], 'E.': [761, 1399, 1477, 1678, 2459, 3087, 3549, 3727, 4367], 'Gregor': [762], 'P.': [763, 2329], 'Kuhar': [764, 884, 904], 'M.': [765, 1076, 1109], 'Uhl': [766, 882, 902, 1518, 1538], 'G.': [767, 782, 802, 1709, 3783], 'Science.': [768, 1046], '1991;': [769], '254:': [770], '576-578Crossref': [771], '(503)': [774], 'Scholar,': [776, 798, 895, 1054, 1074, 1103, 1227, 1319, 1393, 1471, 1529, 3543, 3747, 3767], '4Chen': [777], 'J.-G.': [778], 'Liu-Chen': [779], 'Rudnick': [781, 801], 'J.': [783, 803, 877, 888, 899, 908, 1080, 1093, 1211, 1242, 1303, 1334, 1378, 1404, 1456, 1482, 1683, 1860, 2230, 2331, 2464, 3092, 3166, 3228, 3293, 3528, 3554, 3732, 3752, 3796, 4038, 4372], 'Biol.': [784, 1063, 1095, 1113, 1243, 1335, 1379, 1405, 1457, 1483, 1684, 1861, 2465, 3093, 3167, 3229, 3529, 3555, 3733, 3753, 4373], 'Chem.': [785, 1244, 1336, 1380, 1406, 1458, 1484, 1685, 1862, 2466, 3094, 3168, 3230, 3530, 3556, 3734, 3754, 4374], '1998;': [786, 1064, 1096, 1863, 2246, 3169, 3231], '273:': [787, 1864, 3170, 3232], '12675-12681Abstract': [788], '(125)': [796], '5Androutsellis-Theotokis': [799], 'Neurosci.': [804, 889, 909], '22:': [806], '8370-8378Crossref': [807], 'mammalian': [813], 'brain,': [814], 'all': [819, 971, 2976, 3010], 'parts': [820], 'bodies': [827], 'substantia': [831], 'nigra': [832], 'ventral': [834, 871, 2318], 'tegmental': [835, 872], 'area': [836, 873], 'striatum': [842, 862], 'cerebral': [844], 'cortex.': [845], 'maximal': [847], 'detected': [853, 2735, 4155, 4274, 4352], 'synapses': [855], 'located': [856, 3911, 4063, 4291, 4403], 'along': [857], 'processes': [860, 974], 'distal': [865, 1604], 'regions': [866], '(6Nirenberg': [874], 'M.J.': [875, 885, 897, 905], 'Chan': [876, 898], 'Pohorille': [878], 'Vaughan': [880, 900], 'R.A.': [881, 901], 'G.R.': [883, 903, 1519, 1539], 'Pickel': [886, 906], 'V.M.': [887, 907], '1997;': [890, 910, 1047], '17:': [891, 911], '6899-6907Crossref': [892], '7Nirenberg': [896], '5255-5262Crossref': [912], 'This': [916, 1595], 'specific': [917, 3647], 'targeting': [918], 'normal': [921, 1172, 1568, 3832], 'activity': [922], 'require': [927], 'efficient': [928, 997, 1436, 3626], 'newly': [931], 'synthesized': [932], 'sorting': [937, 998], 'transport': [939, 986, 1621, 3275, 3346, 3654, 3834, 4191], 'carriers': [940], 'Golgi': [943], 'complex,': [944], 'functional': [955, 3721], 'sites.': [956], 'also': [966, 1428, 1508], 'endocytosis.': [969], 'Although': [970, 4438], 'currently': [976], 'being': [977], 'investigated,': [978], 'focused': [983], 'ER.': [991, 1578], 'molecular': [993, 4390, 4410], 'mechanisms': [994], 'multispanning': [1001], 'proteins': [1002, 2443, 2619, 2708, 3029, 3462, 4458], 'coatomer': [1004], 'COPII': [1005], 'vesicles': [1006], 'understood.': [1012], 'Two': [1013, 4345], 'sequence': [1014, 1143], 'motifs': [1015, 1144], 'cytoplasmic': [1018, 1934, 1972], 'domains': [1019, 1505, 1959], 'proteins,': [1022, 1136], 'di-acidic': [1024], '((D/E)X(E/D),': [1025], 'where': [1026], 'X': [1027], 'any': [1029, 4164], 'acid)': [1031], 'di-phenylalanine': [1033], 'motifs,': [1034, 1124], 'have': [1035, 1126, 1164], 'been': [1036, 1127, 1187, 1429, 3079, 3715], 'shown': [1037, 3716, 4301], 'mediate': [1039], '(8Nishimura': [1042], 'N.': [1043, 1058, 3777], 'Balch': [1044, 1059], 'W.E.': [1045, 1060], '277:': [1048], '556-558Crossref': [1049], '(396)': [1052], '9Bannykh': [1055], 'S.I.': [1056], 'Nishimura': [1057], 'Cell': [1062, 1094, 2075, 3018, 3257, 3476, 4174], '8:': [1065], '21-25Abstract': [1066], '(122)': [1072], '10Dominguez': [1075], 'Dejgaard': [1077], 'K.': [1078, 1233, 1325, 1369, 1447, 2217, 3519], 'Fullekrug': [1079], 'Dahan': [1081], 'Fazel': [1083], 'Paccaud': [1085], 'J.-P.': [1086], 'Thomas': [1087], 'D.Y.': [1088], 'Bergeron': [1089], 'J.J.M.': [1090], 'Nilsson': [1091], 'T.': [1092, 1395, 1473, 1535, 1674, 2455, 3083, 3545, 3723, 4363], '140:': [1097], '751-765Crossref': [1098], '(289)': [1101, 3765], '11Sevier': [1104], 'C.S.': [1105], 'Weisz': [1106], 'O.A.': [1107], 'Davis': [1108], 'Machamer': [1110], 'C.E.': [1111], 'Mol.': [1112, 1520, 1540], 'Cell.': [1114], '2000;': [1115, 1522], '11:': [1116], '13-22Crossref': [1117], '(152)': [1120], 'however,': [1125], 'identified': [1128], 'only': [1129], 'limited': [1132], 'number': [1133, 1167, 1499, 3945, 3972], 'it': [1138], 'likely': [1140], 'other': [1142, 3368, 3399, 3879], 'exist': [1145], 'capable': [1148], 'serving': [1150], 'as': [1151, 1726, 2105, 2322, 2451, 3134, 3159, 3221, 3505, 3702, 3810, 4359, 4431, 4433], 'signals.': [1154], 'Recent': [1155], 'studies': [1156, 3259, 3818], 'systems': [1163], 'revealed': [1165, 1597, 3260, 4181], 'requirements': [1169], '(hDAT)': [1185], 'important': [1191, 3651], '(12Torres': [1198, 1290, 3280, 4025], 'G.E.': [1199, 1229, 1291, 1321, 1365, 1443, 3281, 3515, 4026], 'Yao': [1200, 1238, 1292, 1330, 1374, 1452, 3282, 3524, 4027], 'W.D.': [1201, 1293, 3283, 4028], 'Mohn': [1202, 1294, 3284, 4029], 'A.R.': [1203, 1295, 3285, 4030], 'Quan': [1204, 1296, 3286, 4031], 'H.': [1205, 1297, 3287, 4032], 'Kim': [1206, 1298, 3288, 4033], 'K.M.': [1207, 1299, 3289, 3773, 4034], 'Levey': [1208, 1300, 3290, 4035], 'A.I.': [1209, 1301, 3291, 4036], 'Staudinger': [1210, 1302, 3292, 4037], 'Neuron.': [1214, 1306, 3296, 4041], '2001;': [1215, 1307, 2344, 3297, 4042], '30:': [1216, 1308, 3298, 4043], '121-134Abstract': [1217, 1309, 3299, 4044], '(275)': [1225, 1317, 3307, 4052], '13Torres': [1228, 1320], 'Carneiro': [1230, 1322, 1366, 1444, 3516], 'Seamans': [1232, 1324, 1368, 1446, 3518], 'Fiorentini': [1234, 1326, 1370, 1448, 3520], 'C.': [1235, 1327, 1371, 1449, 3521], 'Sweeney': [1236, 1328, 1372, 1450, 3522], 'W.-D.': [1239, 1331, 1375, 1453, 3525], '2003;': [1245, 1337, 1381, 1407, 1459, 1485, 1686, 2467, 3095, 3531, 3557, 3735, 4375], '278:': [1246, 1338, 1382, 1408, 1460, 1486, 1687, 2468, 3096, 3532, 3558, 3736, 4376], '2731-2739Abstract': [1247, 1339, 1383, 1461, 3533], '(212)': [1255, 1347, 1391, 1469, 3541], 'interaction': [1259, 3073], 'residues': [1264, 1608, 1767, 1792, 1813, 1828, 1831, 1883, 1896, 3398, 3421, 3576, 3650], '(LKV620)': [1268], 'PDZ': [1271, 4021], 'PICK1': [1274], '(protein': [1275], 'interacts': [1277], 'Ckinase)': [1279], 'increases': [1280], 'efficiency': [1282], 'ER-to-plasma': [1284], 'contrast,': [1351, 4141], 'lacking': [1353], 'efficiently': [1358, 3824], 'delivered': [1359], '(13Torres': [1364, 1442, 3514], '14Sorkina': [1394, 1472, 3544], 'Doolen': [1396, 1474, 1675, 2456, 3084, 3546, 3724, 4364], 'Galperin': [1398, 1476, 1677, 2458, 3086, 3548, 3726, 4366], 'Zahniser': [1400, 1478, 1679, 2460, 3088, 3550, 3728, 4368], 'N.R.': [1401, 1479, 1680, 2461, 3089, 3551, 3729, 4369], 'Sorkin': [1402, 1480, 1681, 1858, 2462, 3090, 3164, 3226, 3552, 3730, 4370], '28274-28283Abstract': [1409, 1487, 1688, 2469, 3097, 3559, 3737, 4377], '(158)': [1417, 1495, 1696, 2477, 3105, 3567, 3745, 4385], 'oligomerization': [1421], 'proposed': [1430], 'A': [1498, 1892, 2992, 3933, 4266, 4388], 'within': [1502, 3615], 'resulted': [1509], 'inefficient': [1511, 4257], '(15Lin': [1514], 'Z.': [1515, 1531], 'Wang': [1516, 1532, 2332], 'W.': [1517, 1533, 1710], '58:': [1523], '1581-1592Crossref': [1524], '(53)': [1527], '16Lin': [1530], 'Kopajtic': [1534], 'Revay': [1536], 'R.S.': [1537], '1999;': [1542, 3755], '56:': [1543], '434-447Crossref': [1544], '(79)': [1547], 'probably': [1557, 3951], 'consequence': [1559], 'either': [1561, 3322], 'incorrect': [1562], 'folding': [1563], 'disruption': [1565], 'translocation': [1570], 'mutant': [1573, 1822, 2181, 2779, 3485, 3495, 3582, 4144, 4282, 4289, 4448, 4457], 'molecules': [1575, 3950], 'present': [1581], 'performed': [1584, 2717, 2762, 3013, 3142, 3679, 3699, 4209], 'extensive': [1586], 'mutagenesis': [1587, 1732, 3697], 'analysis': [1588, 1596, 3315, 4162, 4179], 'carboxyl-terminal': [1591, 3267, 3397, 3660, 3982], 'tail': [1592, 1973, 3661], 'hDAT.': [1594], 'addition': [1600], 'most': [1603], 'residues,': [1606, 3411], 'several': [1607, 3705], 'region': [1613, 3942], 'types': [1633], 'cells,': [1635, 2187, 2935, 3326, 4117], 'Plasmids': [1647], 'Mutations—YFP-DAT,': [1649], 'CFP-DAT,': [1650], 'YFP-ΔN-DAT': [1651], '(deletion': [1652], 'first': [1655], '65': [1656], 'residues),': [1659], 'YFP-DAT-C′615': [1661], '(truncation': [1662], '5': [1666, 2380, 2645], 'residues)': [1669], 'described': [1671, 2323, 2452, 3080, 3160, 3222, 4360], 'previously': [1672, 2453, 3081, 3161, 3223, 3717, 4019, 4361], '(14Sorkina': [1673, 2454, 3082, 3722, 4362], 'full-length': [1700, 3711], 'hDAT': [1701, 2023, 3271], 'pcDNA3.1': [1703], 'kindly': [1705, 2213], 'provided': [1706, 2214], 'Dr.': [1708, 2216], 'Miller': [1711], '(Emory': [1712], 'University).': [1713], 'Single': [1714], 'made': [1719, 3708], 'using': [1720, 1777, 1818, 1838, 1889, 1939, 2147, 2737, 2763, 2787, 2834, 2999, 3014, 3124, 3143, 3465, 3681, 3700], 'DAT/pEYFP-C1,': [1722], 'ΔN-DAT/pEYFP-C1,': [1723], 'DAT/pcDNA3.1': [1725], 'templates': [1727], 'QuickChange': [1730, 1779], 'site-directed': [1731, 1780, 1819, 1890], 'kit': [1733, 2741], 'according': [1734], "manufacturer's": [1737], 'protocol': [1738], '(Stratagene': [1739], 'Cloning': [1740], 'Systems,': [1741], 'La': [1742], 'Jolla,': [1743], 'CA).': [1744], 'To': [1745, 1841, 2267, 2928, 3050, 3408, 3687, 4198], 'generate': [1746, 1842], 'C′618,': [1748], 'C′615,': [1749], 'C′610,': [1750], 'C′576': [1752], 'truncation': [1753, 3330, 3377, 3581], 'mutants,': [1754, 3413, 4207], 'stop': [1756, 1806, 1836, 1905, 1984], 'codon': [1757, 1807, 1837, 1985], 'created': [1759], 'positions': [1762, 1810], 'corresponding': [1763, 1811, 1879, 1930, 3060], '619,': [1768], '616,': [1769], '611,': [1770], '577,': [1772], 'respectively,': [1773, 1817], 'DAT-pcDNA3.1': [1776], 'mutagenesis.': [1781, 1820, 1840, 1891, 3669], 'C′597-LKV': [1784, 3430], 'C′586-LKV': [1786], 'generated': [1788, 1825, 1980], 'replacing': [1790, 1827], 'following': [1793], 'residue': [1794, 1987, 4014], '597': [1795], '(598–600)': [1796], '586': [1798], '(587–589)': [1799], 'LKV,': [1801], 'creating': [1804], '601': [1814], '590,': [1816], 'Δ581–610': [1823], '581–590': [1829], '611–620': [1832], 'followed': [1833, 1902, 1966, 2514, 2724, 3467], 'Quick-change': [1839], 'receptor': [1845, 1933, 1963, 2065], '(EGFR)-DAT': [1846], 'chimera,': [1847], 'XhoI': [1849, 1909, 1940], 'restriction': [1850], 'site': [1851, 1910, 1918], 'introduced': [1853, 1912], 'wtEGFR-pEGFP-N1': [1855], '(17Carter': [1856, 3162, 3224], 'R.E.': [1857, 3163, 3225], '35000-35007Abstract': [1865, 3171, 3233], '(185)': [1873, 3179, 3241], 'Scholar)': [1875], 'position': [1878], '644–645': [1884], 'EGFR': [1887], 'DNA': [1893], 'fragment': [1894, 1927], 'encoding': [1895], '578–620': [1897], 'codon,': [1906], '5′-end': [1915], 'HindIII': [1917, 1942], '3′-end,': [1921], 'obtained': [1923, 2067, 2996], 'PCR.': [1925], 'wtEGFR-pEGFP-N1(XhoI)': [1929], 'cut': [1937], 'replaced': [1944], 'DAT-(578–620)': [1947], 'fragment.': [1948], 'resultant': [1950], 'EGFR-DAT': [1951, 1991, 2860, 3188], 'chimera': [1952], 'consisted': [1953, 2950], '(residues': [1964, 3662], '1–643)': [1965], 'serine,': [1968], 'arginine,': [1969], 'DAT-(578–620).': [1975], 'EGFR-ΔC': [1977, 3186], 'construct': [1978], 'introducing': [1982], '646': [1988], 'chimera.': [1992], 'both': [1994], 'constructs': [1995], 'GFP': [1996, 2036, 2797], 'reading': [2001], 'frame': [2002], 'translated.': [2006], 'All': [2007, 3139], 'confirmed': [2010], 'automated': [2012], 'dideoxynucleotide': [2013], 'sequencing.': [2014], 'Antibodies—Monoclonal': [2015], 'antibody': [2017, 2034, 2056, 2906], 'against': [2018], 'purchased': [2025], 'Chemicon': [2027], 'International,': [2028], 'Inc.': [2029], '(Temecula,': [2030], 'CA);': [2031], 'polyclonal': [2032, 2661], 'rabbit': [2033], 'Abcam': [2039], 'Ltd.': [2040], '(Cambridge,': [2041], 'UK);': [2042], 'monoclonal': [2043, 2055, 2719], 'mouse': [2044], 'antibodies': [2045, 2721, 2727, 2890], 'α-tau': [2047], 'α-PDI': [2049], 'Transduction': [2052], 'Laboratories.': [2053], 'Mouse': [2054], '528': [2057], 'American': [2069], 'Type': [2070], 'Culture': [2071, 2076], 'Collection': [2072], '(Manassas,': [2073], 'VA).': [2074], 'Transfections—Porcine': [2078], '(PAE)': [2081], 'grown': [2084, 2108, 2137, 2273, 2415, 2445, 2802, 3031, 3191], 'F-12': [2086, 2385], 'medium': [2087, 2113, 2260, 2386], 'containing': [2088, 2114, 2387, 2489, 2840, 2868], '10%': [2089, 2115, 2263, 2388, 2562], 'serum': [2092, 2847, 2871], '(FBS)': [2093], 'antibiotics.': [2095, 2118, 2266], 'Human': [2096], 'cervical': [2097], 'carcinoma': [2098], 'HeLa': [2099, 2186, 3323, 3441], 'HEK293T': [2101], '(referred': [2103], 'HEK293)': [2106], "Dulbecco's": [2110], 'modified': [2111], "Eagle's": [2112], 'FBS': [2116, 2264], 'Entactin-collagen': [2119], 'IV-laminin': [2120], 'coating': [2121], '(Upstate': [2122], 'Biotechnology,': [2123], 'Inc.)': [2124], 'applied': [2126], '35-mm': [2128, 2447], 'dishes': [2129, 2448], 'increase': [2131], 'HEK293': [2132, 3325], 'adherence.': [2134], 'Cells': [2135, 2371, 2399, 2862], '50–80%': [2139], 'confluency': [2140, 2276], 'transfected': [2142, 2184, 2292, 2423, 3929, 4116], 'appropriate': [2145, 2295, 2426], 'plasmids': [2146, 2296, 2427], 'Effectene': [2148], '(Qiagen,': [2149], 'Hilden,': [2150], 'Germany).': [2151], 'For': [2152, 2176], 'microscopy,': [2153], 'split': [2157], '1': [2158, 2494, 2506, 2564, 2567, 2574, 2877, 3219], 'day': [2159], 'after': [2160, 3901, 4099, 4214], 'transfection': [2161, 3902], 'onto': [2162], 'glass': [2163, 2804, 3034], 'coverslips': [2164, 2406, 2805, 2922, 3037], 'experiments': [2169, 2431, 2649, 2858, 3479, 4213, 4277], '2nd': [2172], '3rd': [2174], 'day.': [2175], 'stable': [2177], 'expression,': [2178], 'wild-type': [2179, 2775, 3842, 4237, 4349, 4446, 4466], 'DATs': [2182], 'single': [2190, 3682, 3976], 'clones': [2192], 'selected': [2194], 'growing': [2196], 'presence': [2201], 'G418': [2203], '(400': [2204], 'μg/ml).': [2205], '1RB3AN27': [2211], 'Prasad': [2218, 2237], '(University': [2219], 'Colorado': [2221], 'Health': [2222], 'Sciences': [2223], 'Center)': [2224], '(18Clarkson': [2225], 'E.D.': [2226, 2337], 'Rosa': [2227], 'F.G.L.': [2228], 'Edwards-Prasad': [2229], 'Weiland': [2231], 'D.A.': [2232], 'Witta': [2233], 'S.E.': [2234], 'Freed': [2235, 2338], 'C.R.': [2236, 2339], 'K.N.': [2238], 'Proc.': [2239], 'Natl.': [2240], 'Acad.': [2241], 'U.': [2243], '95:': [2247], '1265-1270Crossref': [2248], '(51)': [2251], 'maintained': [2257, 2298], 'RPMI': [2259], 'supplemented': [2261, 2593, 2677], 'promote': [2268], 'differentiation,': [2269], '30–50%': [2275], 'then': [2278, 2291, 2373, 2422, 2549, 2687, 2864], 'treated': [2279], '2': [2281, 2286, 2300, 2307, 2390, 2432, 2664, 3899, 4215], 'mm': [2282, 2301, 2391, 2491, 2495, 2555, 2558, 2565, 2568, 2571, 2575, 2596, 2680], 'dibutyryl': [2283, 2302], 'cAMP': [2284, 2303], 'days.': [2287, 2310, 2418], 'next': [2306], '3': [2309, 2417, 2434, 4097], 'Primary': [2311], 'derived': [2313], 'E15': [2315], 'mesencephalon': [2319], 'prepared': [2321, 2355, 2816, 3158], '(19Zawada': [2324], 'W.M.': [2325], 'Meintzer': [2326], 'M.K.': [2327], 'Rao': [2328], 'Marotti': [2330], 'X.': [2333], 'Esplen': [2334], 'J.E.': [2335], 'Clarkson': [2336], 'Heidenreich': [2340], 'K.A.': [2341], 'Brain': [2342], 'Res.': [2343], '891:': [2345], '185-196Crossref': [2346], '(39)': [2349], 'Briefly,': [2352, 2480, 3108], 'Ca2+/Mg2+-free': [2357], "Hanks'": [2358], 'balanced': [2359], 'salt': [2360], 'solution': [2361], 'mechanically': [2363], 'dispersing': [2364], 'tissue': [2365], 'pieces': [2366], '1-ml': [2369], 'pipette.': [2370], 'centrifuged': [2374], '1000': [2376], '×': [2377, 2614, 2914], 'g': [2378, 2915], 'min': [2381, 2502, 2611, 2827, 2887], 'resuspended': [2383], 'l-glutamine,': [2392], '100': [2393, 2396, 2679], 'μg/ml': [2394, 2579, 2585], 'streptomycin,': [2395], 'units/ml': [2397], 'penicillin.': [2398], 'plated': [2401], 'polyethylene': [2403], 'imide-coated': [2404], '18-mm': [2405], '12-well': [2409], 'plate,': [2410], '60,000': [2412], 'cells/cm2,': [2413], 'days': [2435, 3900, 4098, 4216], 'post-transfection.': [2436], 'Surface': [2437, 4175], 'Biotinylation': [2438], 'Immunoprecipitation—Cells': [2440], 'expressing': [2441, 3025, 3185], 'biotinylated': [2450, 2618, 3461, 4236, 4246, 4271], 'washed': [2484, 2527, 2542, 2626, 2672, 2682, 2807], 'cold': [2486, 2530], 'saline': [2488], '0.1': [2490, 2536], 'CaCl2': [2492], 'MgCl2': [2496], '(PBS)': [2497], 'incubated': [2499, 2532, 2865, 2884, 3453], '20': [2501, 2917], 'ice': [2504, 2534], 'mg/ml': [2507], 'sulfo-N-hydroxysuccinimidobiotin': [2508], '(EZ-Link™': [2509], 'sulfo-NHS-biotin,': [2510], 'Pierce)': [2511], 'incubation': [2518, 2837], 'fresh': [2520], 'sulfo-NHS-biotin.': [2521], 'After': [2522, 2919], 'biotinylation,': [2523], 'twice': [2528, 2673], 'm': [2537], 'glycine': [2538], 'PBS': [2544, 2811], 'again.': [2545], 'solubilized': [2550], 'lysis': [2552, 2630, 2675], 'buffer': [2553, 2640, 2676], '(50': [2554], 'NaCl,': [2556, 2681, 2685], '50': [2557, 3212], 'HEPES,': [2559], 'pH': [2560], '7.2,': [2561], 'glycerol,': [2563], 'EGTA,': [2566], 'EDTA,': [2569], '10': [2570, 2578, 2584, 2595, 2610, 3337, 3575], 'sodium': [2572, 2591], 'fluoride,': [2573, 2577], 'phenylmethylsulfonyl': [2576], 'leupeptin,': [2580], '544': [2581], 'μm': [2582], 'iodoacetamide,': [2583], 'aprotinin,': [2586], '1%': [2587, 2590], 'Triton': [2588, 2842], 'X-100,': [2589], 'deoxycholate)': [2592], 'Tris-HCl': [2597], '(pH': [2598], '6.8)': [2599], '4': [2601, 2667, 3216], '°C.': [2602, 2668], 'lysates': [2604], 'cleared': [2606], 'centrifugation': [2608, 2911], '16,000': [2613], 'g,': [2615], 'precipitated': [2621, 3464], 'NeutrAvidin™': [2623], 'beads': [2624, 2637, 2696, 4227, 4358], '(Pierce),': [2625], 'five': [2627], 'times': [2628], 'buffer,': [2631], 'denatured': [2633, 2688], 'heating': [2635, 2690], 'sample': [2639, 2692], '95': [2642], '°C': [2643, 3217], 'min.': [2646, 2918], 'some': [2648, 4276], 'supernatants': [2650, 4224, 4354], 'NeutrAvidin': [2653, 2695, 4226, 4357], 'precipitation': [2654], 'further': [2656, 4009], 'subjected': [2657, 3665], 'immunoprecipitation': [2659], 'anti-GFP': [2662, 4229], 'h': [2665, 2878, 3220], 'precipitates': [2670], 'once': [2683], 'without': [2684], 'buffer.': [2693], 'immunoprecipitates': [2698, 2791], 'analyzed': [2700, 2748, 3898], 'electrophoresis': [2702], '7.5%': [2704], 'SDS-PAGE,': [2705], 'transferred': [2710], 'nitrocellulose': [2712], 'Western': [2714, 3474], 'blotting': [2715], 'secondary': [2726, 2889, 2905], 'conjugated': [2728], 'horseradish': [2730], 'peroxidase,': [2731], 'bands': [2733], 'enhanced': [2739], 'chemiluminescence': [2740, 2756], 'Pierce.': [2743], 'Several': [2744], 'x-ray': [2745], 'films': [2746], 'determine': [2750], 'linear': [2752], 'range': [2753], 'signals,': [2757], 'quantifications': [2760], 'densitometry': [2764], 'NIH': [2766], 'Image': [2767], 'software.': [2768, 2981, 3153], 'co-immunoprecipitation': [2770], 'experiments,': [2771], 'co-expressing': [2774], 'YFP-ΔN-DAT-G585A': [2778], 'lysed,': [2781], 'immunoprecipitated': [2785, 4222], 'anti-DAT.': [2789], 'blotted': [2793], 'antibodies.': [2798, 4230], 'Immunofluorescence': [2799], 'Staining—The': [2800], 'Mg2+-free': [2810], '(CMF-PBS),': [2812], 'fixed': [2813], 'freshly': [2815], '4%': [2817], 'paraformaldehyde': [2818], '(Electron': [2819], 'Microscopy': [2820, 3019], 'Sciences,': [2821], 'Ft.': [2822], 'Washington,': [2823], 'PA)': [2824], '15': [2826], 'room': [2829, 2850, 2874], 'temperature,': [2830], 'mildly': [2832], 'permeabilized': [2833], '3-min': [2836], 'CMF-PBS': [2839, 2867], '0.1%': [2841], 'X-100': [2843], '0.5%': [2845, 2869], 'albumin': [2848, 2872], 'temperature.': [2851], 'permeabilization': [2853], 'step': [2854], 'omitted': [2856], 'chimeras.': [2861], 'temperature': [2875], 'antibodies,': [2881], 'subsequently': [2883], '30': [2886], 'labeled': [2891], 'CY3': [2893], 'CY5': [2895], '(The': [2896], 'Jackson': [2897], 'Laboratory,': [2898], 'West': [2899], 'Grove,': [2900], 'PA).': [2901], 'solutions': [2907], 'precleared': [2909], '100,000': [2913], 'staining,': [2920], 'mounted': [2924, 3039], 'Mowiol': [2926], '(Calbiochem).': [2927], 'obtain': [2929], 'high': [2930, 3848], 'resolution': [2931], 'three-dimensional': [2932], 'images': [2933, 2986, 2995, 3011, 3056, 3113, 3131], 'imaging': [2938], 'Mariannas': [2939], 'work': [2940], 'station': [2941], '(Intelligent': [2942], 'Imaging': [2943], 'Innovation,': [2944], 'Denver,': [2945], 'CO)': [2946], 'used,': [2948], 'Zeiss': [2953], 'inverted': [2954], 'microscope': [2955, 3042, 3048], 'equipped': [2956], 'cooled': [2959], 'CCD': [2960], 'CoolSnap': [2961], 'HQ': [2962], '(Roper,': [2963], 'CA),': [2964], 'z-step': [2965], 'motor,': [2966], 'dual': [2967], 'filter': [2968, 3061], 'wheels,': [2969], 'xenon': [2972], '175-watt': [2973], 'light': [2974, 4318], 'source,': [2975], 'controlled': [2977], 'SlideBook': [2979, 3152], '4.0': [2980], 'Typically,': [2982], '5–15': [2983], 'serial': [2984], 'two-dimensional': [2985], 'recorded': [2988], '200-nm': [2990], 'intervals.': [2991], 'z-stack': [2993], 'deconvoluted': [2998], 'modification': [3001], 'constrained': [3004], 'iteration': [3005], 'method.': [3006], 'Final': [3007], 'arrangement': [3008], 'Adobe': [3015], 'Photoshop.': [3016], 'FRET': [3021, 3067, 3112, 3120, 3148], 'Measurements—PAE': [3022], 'transiently': [3024, 3184, 3674, 3820], 'various': [3026], 'YFP-tagged': [3027], '25-mm': [3033], 'coverslips.': [3035], 'chamber': [3043], 'placed': [3045], 'stage.': [3049], 'visualize': [3051], 'CFP': [3052], 'YFP,': [3054, 3109], 'consecutive': [3055], 'acquired': [3058], 'channels.': [3062], 'method': [3064], 'sensitized': [3066], 'measurement': [3068], 'examine': [3071], 'between': [3074], 'YFP-': [3075], 'CFP-DAT': [3077], 'acquired,': [3115], 'background-subtracted,': [3116], '(FRETC)': [3121], 'calculated': [3123], 'CFP/FRET': [3125], 'YFP/FRET': [3127], 'cross-bleed': [3128], 'coefficients.': [3129], 'FRETC': [3130], 'presented': [3133], 'intensity-modulated': [3136], 'pseudocolor': [3137], 'images.': [3138], 'calculations': [3140], 'Channel': [3145], 'Math': [3146], 'modules': [3149], 'Binding': [3155], 'Assay—125I-EGF': [3156], 'PAE': [3182, 3806, 3867, 3895], 'chimeras': [3189], '6-well': [3193], 'dishes,': [3194], 'binding': [3200, 4023], 'measured': [3207], 'incubating': [3209], 'ng/ml': [3213], '125I-EGF': [3214], 'LKV': [3244, 3401], 'Motif': [3245], 'Is': [3246], 'Not': [3247], 'Sufficient': [3248], 'Efficient': [3251], 'Transport': [3252], 'Surface—Previous': [3258], 'importance': [3262], 'acids': [3269, 3339], '(618LKV620)': [3272], 'However,': [3310], 'initial': [3312], 'deletion': [3318], 'expressed': [3320, 3439, 3488, 3504, 3675, 3821, 3845], 'noticed': [3328], '2,': [3334], '5,': [3335], '(Fig.': [3340, 3498, 3931, 4090, 4138], '1A)': [3341], 'abrogate': [3345], 'surface.': [3352], 'Rather,': [3353], 'truncations': [3355], 'substantially': [3356], '(data': [3363, 3388, 3593], 'shown).': [3365, 3390, 3595], 'On': [3366, 3839], 'hand,': [3369], 'removal': [3370], 'entire': [3373], '(C′576': [3376], 'mutant)': [3378], 'results': [3392, 3597], 'suggested': [3393, 3598], 'there': [3395], 'than': [3400, 4316, 4462], 'trafficking.': [3407], 'map': [3409], '23': [3418], '34': [3420], 'truncated': [3423], 'LKV620': [3426], 'motif': [3427, 3602, 4024], 'attached,': [3429], 'C′586-LKV,': [3432], 'respectively': [3433], '(see': [3434], 'Fig.': [3435, 3989, 4234, 4284, 4303], '1A),': [3436], 'stably': [3438], 'cells.': [3442, 3838, 3864, 3947], 'order': [3444, 3643], 'label': [3446], 'molecules,': [3449], 'membrane-impermeable': [3456], 'NHS-biotin': [3457], 'derivative,': [3458], 'NeutrAvidin-agarose': [3466], 'detection': [3470, 4328], 'blotting.': [3475], 'whereas': [3482, 4242], 'C′597-KLV': [3484], 'partially': [3487], 'membrane,': [3492], 'C′586-KLV': [3494], 'undetectable': [3497], '1B).': [3499], 'mainly': [3503], 'nonglycosylated': [3507, 4399, 4443], 'form': [3508, 4392, 4400, 4412, 4422, 4444], '(∼50': [3509], 'kDa)': [3510, 4394, 4414], 'inaccessible': [3511], 'Moreover,': [3570], 'attachment': [3571], '(611–620)': [3577], 'DAT-C′577': [3580], '(Δ578–610)': [3583], 'restore': [3586], 'PDZ-binding': [3601], 'sufficient': [3605], 'juxtamembrane': [3617], 'portion': [3618], 'Analysis': [3634], 'Alanine': [3637], 'Substitution': [3638], 'Mutants': [3639, 4171], 'Fluorescence': [3641], 'Microscopy—In': [3642], 'identify': [3645], 'surface,': [3658], '575–620)': [3663], 'alanine-scanning': [3668], 'phenotypic': [3671], 'screening': [3672], 'analysis.': [3686], 'facilitate': [3688], 'visualization': [3690, 3874], 'YFP-DAT': [3701, 3713, 3890, 3909, 3936, 4093, 4206, 4220, 4238, 4335, 4350, 4426, 4449], 'template,': [3703], 'although': [3704], 'untagged': [3710], 'DAT.': [3712], 'fully': [3720], '20Daniels': [3748], 'G.M.': [3749], 'Amara': [3750], 'S.G.': [3751], '274:': [3756], '35794-35801Abstract': [3757], '21Carvelli': [3768], 'L.': [3769], 'Moron': [3770], 'J.A.': [3771, 3791], 'Kahlig': [3772], 'Ferrer': [3774], 'J.V.': [3775], 'Sen': [3776], 'Lechleiter': [3778], 'Leeb-Lundberg': [3780], 'L.M.': [3781, 3787], 'Merrill': [3782], 'Lafer': [3784], 'E.M.': [3785], 'Ballou': [3786], 'Shippenberg': [3788], 'Javitch': [3790], 'R.Z.': [3793], 'Galli': [3794], 'Neurochem.': [3797], '81:': [3799], '859-869Crossref': [3800], '(174)': [3803], 'chosen': [3809], 'our': [3811], 'preferred': [3812], 'structure-function': [3817], 'targeted': [3825], 'displays': [3831], 'characteristics': [3835], 'contrary,': [3841], 'very': [3847, 4267, 4331], 'levels': [3849], 'retained': [3851], 'large': [3857], 'population': [3858], 'HeLa,': [3860], 'HEK293,': [3861], 'COS-1': [3863], 'addition,': [3866], 'flattened': [3870], 'allow': [3872, 4326], 'clear': [3873, 4327], 'organelles': [3880], 'living': [3882], 'epifluorescence': [3885], 'microscopy.': [3886, 3907], 'localization': [3888, 3967, 3995, 4089, 4094, 4106, 4151], 'live': [3904], 'Wild-type': [3908], 'enrichment': [3917], 'ruffles,': [3919], 'phylopodia,': [3920], 'protrusions': [3923], 'majority': [3926], '(>80%)': [3927], '2).': [3932, 4091, 4139], 'pool': [3934], 'seen': [3938, 4076], 'perinuclear': [3941], 'represent': [3952], 'Golgi-': [3954], 'ER-associated': [3956], 'way': [3960], 'Similar': [3965], 'subcellular': [3966], 'observed': [3969, 4112], 'multiple': [3978], 'residues.': [3983], 'summarized': [3987], '1A.': [3990], 'exceptions': [3992], 'D600A,': [4000], 'K619A': [4002, 4005], 'mutants.': [4003], 'investigated': [4010], 'lysine': [4013], 'part': [4017], 'established': [4020], 'almost': [4064, 4292], 'exclusively': [4065, 4293], 'intracellular': [4068], 'tubular': [4069], 'network': [4070], 'throughout': [4071], 'often': [4075], 'associated': [4077], 'nuclear': [4080], 'envelope,': [4081], 'pattern': [4083, 4148], 'distribution': [4085], 'When': [4092], 'inspected': [4096], 'transfection,': [4100], 'clearly': [4102], 'distinguishable': [4103], 'K590A': [4108, 4247], '20–30%': [4114], 'suggesting': [4118], 'block,': [4125], 'but': [4126], 'rather': [4127], 'slowed': [4128], 'down,': [4129], 'G585A': [4143, 4272], 'displayed': [4145], 'characteristic': [4147], 'microscopic': [4161], 'under': [4163], 'conditions.': [4165], 'Plasma': [4166], 'Membrane': [4167], 'Expression': [4168], 'Analyzed': [4172], 'Biotinylation—Our': [4176], 'had': [4180], 'Gly-585,': [4185], 'Lys-590,': [4186], 'analyze': [4199], 'quantitatively': [4200], 'expression.': [4218], 'Nonbiotinylated': [4219], 'As': [4231], 'illustrated': [4232], '3,': [4235], 'readily': [4240], 'detected,': [4241], 'amounts': [4244, 4440, 4454], 'much': [4252], 'smaller,': [4253], 'indicative': [4254], 'small': [4268], '(<0.5%': [4278], 'total': [4280], 'cellular': [4281], 'proteins;': [4283], '3),': [4285], 'indicating': [4286], 'intracellularly,': [4294], 'agreement': [4296, 4469], '2.': [4304], 'general,': [4306], 'assay': [4308], 'more': [4310], 'sensitive': [4311], 'detecting': [4313], 'latter': [4322], 'technique': [4323], 'does': [4324], 'weak': [4332], 'diffusely': [4336], 'distributed': [4337], 'plane': [4340], 'main': [4346], 'forms': [4347], 'lower': [4389, 4461], 'weight': [4391, 4411], '(∼75': [4393], 'corresponds': [4395, 4415], 'immature,': [4398], 'higher': [4409], '(∼105': [4413], 'mature': [4418, 4421], 'YFP-DAT.': [4419, 4437, 4467], 'represents': [4423], 'biotinylated,': [4430], 'well': [4432], 'endosomal': [4434], 'trans-Golgi': [4436], 'comparable,': [4451], 'relative': [4453], 'matured': [4456]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2001971849', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 1}, {'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 4}, {'year': 2019, 'cited_by_count': 1}, {'year': 2018, 'cited_by_count': 2}, {'year': 2017, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 2}, {'year': 2015, 'cited_by_count': 3}, {'year': 2014, 'cited_by_count': 2}, {'year': 2013, 'cited_by_count': 2}, {'year': 2012, 'cited_by_count': 1}], 'updated_date': '2024-12-10T05:26:17.832413', 'created_date': '2016-06-24'}
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