Title: Amperometric determination of choline with use of immobilized choline oxidase
Abstract: Abstract Choline oxidase (choline: oxygen oxidoreductaserpar; was immobilized on a partially aminated polyacrylonitrile membrane. The enzyme electrode, consisting of an immobilized‐enzyme membrane and an oxygen probe, was employed for the determination choline. Dissolved oxygen consumption by the enzymatic reaction was measured amperometrically. The rate assay method was used for the choline determination. The response time of the sensor was 7 sec for choline. The choline assay was done within 1 min. The choline calibration curve was linear from 0 to 0.1m M . The response was reproducible within an average relative error of 2.3% when 0.2m M choline was employed for experiments. The choline in the fermentation media was determined by the sensor. Furthermore, phospholipids in the serum were also determined with native phospholiphase D and the enzyme electrode.
Publication Year: 1980
Publication Date: 1980-05-01
Language: en
Type: article
Indexed In: ['crossref']
Access and Citation
Cited By Count: 32
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot