Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W2000989488', 'doi': 'https://doi.org/10.1111/j.1365-2958.2007.05980.x', 'title': 'Characterization of a tightly controlled promoter of the halophilic archaeon <i>Haloferax volcanii</i> and its use in the analysis of the essential <i>cct1</i> gene', 'display_name': 'Characterization of a tightly controlled promoter of the halophilic archaeon <i>Haloferax volcanii</i> and its use in the analysis of the essential <i>cct1</i> gene', 'publication_year': 2007, 'publication_date': '2007-10-31', 'ids': {'openalex': 'https://openalex.org/W2000989488', 'doi': 'https://doi.org/10.1111/j.1365-2958.2007.05980.x', 'mag': '2000989488', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/17973910'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1111/j.1365-2958.2007.05980.x', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S102549058', 'display_name': 'Molecular Microbiology', 'issn_l': '0950-382X', 'issn': ['0950-382X', '1365-2958'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5080890019', 'display_name': 'Andrew T. Large', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I79619799', 'display_name': 'University of Birmingham', 'ror': 'https://ror.org/03angcq70', 'country_code': 'GB', 'type': 'education', 'lineage': ['https://openalex.org/I79619799']}], 'countries': ['GB'], 'is_corresponding': True, 'raw_author_name': 'Andrew Large', 'raw_affiliation_strings': ['School of Biosciences, The University of Birmingham, Edgbaston B15\u20032TT, UK.'], 'affiliations': [{'raw_affiliation_string': 'School of Biosciences, The University of Birmingham, Edgbaston B15\u20032TT, UK.', 'institution_ids': ['https://openalex.org/I79619799']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5050871757', 'display_name': 'Claudia Stamme', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I114090438', 'display_name': 'Goethe University Frankfurt', 'ror': 'https://ror.org/04cvxnb49', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I114090438']}], 'countries': ['DE'], 'is_corresponding': True, 'raw_author_name': 'Claudia Stamme', 'raw_affiliation_strings': ['J. W. Goethe Universität, Biozentrum Niederursel, Institut für Molekulare Biowissenschaften, Max-von-Laue-Str. 9, D60438 Frankfurt, Germany.'], 'affiliations': [{'raw_affiliation_string': 'J. W. Goethe Universität, Biozentrum Niederursel, Institut für Molekulare Biowissenschaften, Max-von-Laue-Str. 9, D60438 Frankfurt, Germany.', 'institution_ids': ['https://openalex.org/I114090438']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5101436003', 'display_name': 'Christian Lange', 'orcid': 'https://orcid.org/0000-0002-7582-0076'}, 'institutions': [{'id': 'https://openalex.org/I114090438', 'display_name': 'Goethe University Frankfurt', 'ror': 'https://ror.org/04cvxnb49', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I114090438']}], 'countries': ['DE'], 'is_corresponding': True, 'raw_author_name': 'Christian Lange', 'raw_affiliation_strings': ['J. W. Goethe Universität, Biozentrum Niederursel, Institut für Molekulare Biowissenschaften, Max-von-Laue-Str. 9, D60438 Frankfurt, Germany.'], 'affiliations': [{'raw_affiliation_string': 'J. W. Goethe Universität, Biozentrum Niederursel, Institut für Molekulare Biowissenschaften, Max-von-Laue-Str. 9, D60438 Frankfurt, Germany.', 'institution_ids': ['https://openalex.org/I114090438']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5091392826', 'display_name': 'Zengqiang Duan', 'orcid': 'https://orcid.org/0000-0002-5964-7488'}, 'institutions': [{'id': 'https://openalex.org/I4210137227', 'display_name': "Queen's Medical Centre", 'ror': 'https://ror.org/03ap6wx93', 'country_code': 'GB', 'type': 'healthcare', 'lineage': ['https://openalex.org/I1334287468', 'https://openalex.org/I4210137227']}, {'id': 'https://openalex.org/I142263535', 'display_name': 'University of Nottingham', 'ror': 'https://ror.org/01ee9ar58', 'country_code': 'GB', 'type': 'education', 'lineage': ['https://openalex.org/I142263535']}], 'countries': ['GB'], 'is_corresponding': True, 'raw_author_name': 'Zhenhong Duan', 'raw_affiliation_strings': ["Institute of Genetics, University of Nottingham, Queen's Medical Centre, Nottingham NG7\u20032UH, UK."], 'affiliations': [{'raw_affiliation_string': "Institute of Genetics, University of Nottingham, Queen's Medical Centre, Nottingham NG7\u20032UH, UK.", 'institution_ids': ['https://openalex.org/I4210137227', 'https://openalex.org/I142263535']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5071947773', 'display_name': 'Thorsten Allers', 'orcid': 'https://orcid.org/0000-0002-7308-3332'}, 'institutions': [{'id': 'https://openalex.org/I4210137227', 'display_name': "Queen's Medical Centre", 'ror': 'https://ror.org/03ap6wx93', 'country_code': 'GB', 'type': 'healthcare', 'lineage': ['https://openalex.org/I1334287468', 'https://openalex.org/I4210137227']}, {'id': 'https://openalex.org/I142263535', 'display_name': 'University of Nottingham', 'ror': 'https://ror.org/01ee9ar58', 'country_code': 'GB', 'type': 'education', 'lineage': ['https://openalex.org/I142263535']}], 'countries': ['GB'], 'is_corresponding': True, 'raw_author_name': 'Thorsten Allers', 'raw_affiliation_strings': ["Institute of Genetics, University of Nottingham, Queen's Medical Centre, Nottingham NG7\u20032UH, UK."], 'affiliations': [{'raw_affiliation_string': "Institute of Genetics, University of Nottingham, Queen's Medical Centre, Nottingham NG7\u20032UH, UK.", 'institution_ids': ['https://openalex.org/I4210137227', 'https://openalex.org/I142263535']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5073631830', 'display_name': 'Jörg Soppa', 'orcid': 'https://orcid.org/0000-0002-6132-5840'}, 'institutions': [{'id': 'https://openalex.org/I114090438', 'display_name': 'Goethe University Frankfurt', 'ror': 'https://ror.org/04cvxnb49', 'country_code': 'DE', 'type': 'education', 'lineage': ['https://openalex.org/I114090438']}], 'countries': ['DE'], 'is_corresponding': True, 'raw_author_name': 'Jörg Soppa', 'raw_affiliation_strings': ['J. W. Goethe Universität, Biozentrum Niederursel, Institut für Molekulare Biowissenschaften, Max-von-Laue-Str. 9, D60438 Frankfurt, Germany.'], 'affiliations': [{'raw_affiliation_string': 'J. W. Goethe Universität, Biozentrum Niederursel, Institut für Molekulare Biowissenschaften, Max-von-Laue-Str. 9, D60438 Frankfurt, Germany.', 'institution_ids': ['https://openalex.org/I114090438']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5046761439', 'display_name': 'Peter W. M. Hermans', 'orcid': 'https://orcid.org/0000-0002-6381-8526'}, 'institutions': [{'id': 'https://openalex.org/I79619799', 'display_name': 'University of Birmingham', 'ror': 'https://ror.org/03angcq70', 'country_code': 'GB', 'type': 'education', 'lineage': ['https://openalex.org/I79619799']}], 'countries': ['GB'], 'is_corresponding': True, 'raw_author_name': 'Peter A. Lund', 'raw_affiliation_strings': ['School of Biosciences, The University of Birmingham, Edgbaston B15\u20032TT, UK.'], 'affiliations': [{'raw_affiliation_string': 'School of Biosciences, The University of Birmingham, Edgbaston B15\u20032TT, UK.', 'institution_ids': ['https://openalex.org/I79619799']}]}], 'countries_distinct_count': 2, 'institutions_distinct_count': 4, 'corresponding_author_ids': ['https://openalex.org/A5080890019', 'https://openalex.org/A5050871757', 'https://openalex.org/A5101436003', 'https://openalex.org/A5091392826', 'https://openalex.org/A5071947773', 'https://openalex.org/A5073631830', 'https://openalex.org/A5046761439'], 'corresponding_institution_ids': ['https://openalex.org/I79619799', 'https://openalex.org/I114090438', 'https://openalex.org/I114090438', 'https://openalex.org/I4210137227', 'https://openalex.org/I142263535', 'https://openalex.org/I4210137227', 'https://openalex.org/I142263535', 'https://openalex.org/I114090438', 'https://openalex.org/I79619799'], 'apc_list': {'value': 4020, 'currency': 'USD', 'value_usd': 4020, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 2.751, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 91, 'citation_normalized_percentile': {'value': 0.999969, 'is_in_top_1_percent': True, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 96, 'max': 97}, 'biblio': {'volume': '66', 'issue': '5', 'first_page': '1092', 'last_page': '1106'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11162', 'display_name': 'Macromolecular Crystallography Techniques', 'score': 0.9995, 'subfield': {'id': 'https://openalex.org/subfields/2505', 'display_name': 'Materials Chemistry'}, 'field': {'id': 'https://openalex.org/fields/25', 'display_name': 'Materials Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11162', 'display_name': 'Macromolecular Crystallography Techniques', 'score': 0.9995, 'subfield': {'id': 'https://openalex.org/subfields/2505', 'display_name': 'Materials Chemistry'}, 'field': {'id': 'https://openalex.org/fields/25', 'display_name': 'Materials Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T10044', 'display_name': 'Protein Structure Prediction and Analysis', 'score': 0.9972, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10120', 'display_name': 'Bacterial Physiology and Genetics', 'score': 0.9938, 'subfield': {'id': 'https://openalex.org/subfields/1311', 'display_name': 'Genetics'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/haloferax-volcanii', 'display_name': 'Haloferax volcanii', 'score': 0.9826795}, {'id': 'https://openalex.org/keywords/rna-regulation', 'display_name': 'RNA Regulation', 'score': 0.478542}, {'id': 'https://openalex.org/keywords/heterologous-expression', 'display_name': 'Heterologous expression', 'score': 0.42637527}], 'concepts': [{'id': 'https://openalex.org/C2776179084', 'wikidata': 'https://www.wikidata.org/wiki/Q16982678', 'display_name': 'Haloferax volcanii', 'level': 4, 'score': 0.9826795}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.85377103}, {'id': 'https://openalex.org/C101762097', 'wikidata': 'https://www.wikidata.org/wiki/Q224093', 'display_name': 'Promoter', 'level': 4, 'score': 0.65261686}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.65179783}, {'id': 'https://openalex.org/C150194340', 'wikidata': 'https://www.wikidata.org/wiki/Q26972', 'display_name': 'Gene expression', 'level': 3, 'score': 0.47638363}, {'id': 'https://openalex.org/C161733203', 'wikidata': 'https://www.wikidata.org/wiki/Q911828', 'display_name': 'Reporter gene', 'level': 4, 'score': 0.47482833}, {'id': 'https://openalex.org/C2776706248', 'wikidata': 'https://www.wikidata.org/wiki/Q181003', 'display_name': 'Tryptophan', 'level': 3, 'score': 0.4589151}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.43230304}, {'id': 'https://openalex.org/C108305142', 'wikidata': 'https://www.wikidata.org/wiki/Q3062286', 'display_name': 'Heterologous expression', 'level': 4, 'score': 0.42637527}, {'id': 'https://openalex.org/C165864922', 'wikidata': 'https://www.wikidata.org/wiki/Q411391', 'display_name': 'Regulation of gene expression', 'level': 3, 'score': 0.42394727}, {'id': 'https://openalex.org/C143065580', 'wikidata': 'https://www.wikidata.org/wiki/Q3285695', 'display_name': 'Mutant', 'level': 3, 'score': 0.4157263}, {'id': 'https://openalex.org/C550995028', 'wikidata': 'https://www.wikidata.org/wiki/Q10872', 'display_name': 'Archaea', 'level': 3, 'score': 0.4066282}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.3789458}, {'id': 'https://openalex.org/C515207424', 'wikidata': 'https://www.wikidata.org/wiki/Q8066', 'display_name': 'Amino acid', 'level': 2, 'score': 0.21195409}, {'id': 'https://openalex.org/C40767141', 'wikidata': 'https://www.wikidata.org/wiki/Q285697', 'display_name': 'Recombinant DNA', 'level': 3, 'score': 0.06335524}], 'mesh': [{'descriptor_ui': 'D019843', 'descriptor_name': 'Archaeal Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D019848', 'descriptor_name': 'Gene Expression Regulation, Archaeal', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D005822', 'descriptor_name': 'Genetic Vectors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D019622', 'descriptor_name': 'Haloferax volcanii', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D006360', 'descriptor_name': 'Heat-Shock Proteins', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D008967', 'descriptor_name': 'Molecular Biology', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D018832', 'descriptor_name': 'Molecular Chaperones', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D011401', 'descriptor_name': 'Promoter Regions, Genetic', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D019843', 'descriptor_name': 'Archaeal Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D020869', 'descriptor_name': 'Gene Expression Profiling', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D020043', 'descriptor_name': 'Genes, Essential', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017930', 'descriptor_name': 'Genes, Reporter', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D019622', 'descriptor_name': 'Haloferax volcanii', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006360', 'descriptor_name': 'Heat-Shock Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008967', 'descriptor_name': 'Molecular Biology', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D018832', 'descriptor_name': 'Molecular Chaperones', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D020411', 'descriptor_name': 'Oligonucleotide Array Sequence Analysis', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D019642', 'descriptor_name': 'RNA, Archaeal', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D019642', 'descriptor_name': 'RNA, Archaeal', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012333', 'descriptor_name': 'RNA, Messenger', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012333', 'descriptor_name': 'RNA, Messenger', 'qualifier_ui': 'Q000096', 'qualifier_name': 'biosynthesis', 'is_major_topic': False}, {'descriptor_ui': 'D020133', 'descriptor_name': 'Reverse Transcriptase Polymerase Chain Reaction', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014364', 'descriptor_name': 'Tryptophan', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D014364', 'descriptor_name': 'Tryptophan', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014368', 'descriptor_name': 'Tryptophanase', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D014368', 'descriptor_name': 'Tryptophanase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1111/j.1365-2958.2007.05980.x', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S102549058', 'display_name': 'Molecular Microbiology', 'issn_l': '0950-382X', 'issn': ['0950-382X', '1365-2958'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320595', 'host_organization_name': 'Wiley', 'host_organization_lineage': ['https://openalex.org/P4310320595'], 'host_organization_lineage_names': ['Wiley'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/17973910', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 41, 'referenced_works': ['https://openalex.org/W1495893488', 'https://openalex.org/W1560558454', 'https://openalex.org/W1584653951', 'https://openalex.org/W1797990033', 'https://openalex.org/W1908076951', 'https://openalex.org/W1967723054', 'https://openalex.org/W1971566996', 'https://openalex.org/W1987383794', 'https://openalex.org/W1991568949', 'https://openalex.org/W1999710371', 'https://openalex.org/W2014206081', 'https://openalex.org/W2028322882', 'https://openalex.org/W2031753266', 'https://openalex.org/W2035043664', 'https://openalex.org/W2077222517', 'https://openalex.org/W2082334463', 'https://openalex.org/W2083462206', 'https://openalex.org/W2090585110', 'https://openalex.org/W2095768111', 'https://openalex.org/W2098670258', 'https://openalex.org/W2099013682', 'https://openalex.org/W2100986312', 'https://openalex.org/W2106882534', 'https://openalex.org/W2107277218', 'https://openalex.org/W2107633677', 'https://openalex.org/W2113136528', 'https://openalex.org/W2116735948', 'https://openalex.org/W2135320834', 'https://openalex.org/W2137909527', 'https://openalex.org/W2147531155', 'https://openalex.org/W2150297520', 'https://openalex.org/W2151487235', 'https://openalex.org/W2157201523', 'https://openalex.org/W2159555287', 'https://openalex.org/W2160084442', 'https://openalex.org/W2161136014', 'https://openalex.org/W2166669307', 'https://openalex.org/W2166865790', 'https://openalex.org/W2804007336', 'https://openalex.org/W3199943451', 'https://openalex.org/W4233019229'], 'related_works': ['https://openalex.org/W4386457921', 'https://openalex.org/W35743028', 'https://openalex.org/W3047005611', 'https://openalex.org/W2981463511', 'https://openalex.org/W2754857012', 'https://openalex.org/W2620968636', 'https://openalex.org/W2589975841', 'https://openalex.org/W2187042973', 'https://openalex.org/W2127546097', 'https://openalex.org/W1998555791'], 'abstract_inverted_index': {'A': [0], 'system': [1], 'where': [2], 'archaeal': [3], 'gene': [4, 92, 104, 154], 'expression': [5, 61, 93], 'could': [6], 'be': [7, 84, 149], 'controlled': [8, 29], 'by': [9], 'simple': [10], 'manipulation': [11, 42], 'of': [12, 19, 31, 43, 60, 89, 99, 113, 176, 178, 189], 'growth': [13], 'conditions': [14], 'would': [15], 'enable': [16], 'the': [17, 28, 40, 67, 73, 95, 111, 170, 174, 179, 187], 'construction': [18], 'conditional': [20], 'lethal': [21], 'mutants': [22], 'in': [23, 33, 62, 94, 110, 156, 161, 195], 'essential': [24, 180], 'genes,': [25], 'and': [26, 97, 121, 142, 159, 184], 'permit': [27], 'overproduction': [30], 'proteins': [32, 192], 'their': [34], 'native': [35], 'host.': [36], 'As': [37], 'tools': [38], 'for': [39, 72, 152], 'genetic': [41], 'Haloferax': [44], 'volcanii': [45, 91, 158], 'are': [46], 'well': [47], 'developed,': [48], 'we': [49, 78, 168], 'set': [50], 'out': [51], 'to': [52, 75, 124, 172, 185, 193], 'identify': [53], 'promoters': [54, 82], 'with': [55], 'a': [56, 102, 117], 'wide': [57], 'dynamic': [58], 'range': [59], 'this': [63, 146], 'organism.': [64], 'Tryptophan': [65], 'is': [66], 'most': [68], 'costly': [69], 'amino': [70], 'acid': [71], 'cell': [74], 'make,': [76], 'so': [77], 'reasoned': [79], 'that': [80, 106], 'tryptophan-regulated': [81], 'might': [83], 'good': [85], 'candidates.': [86], 'Microarray': [87], 'analysis': [88], 'H.': [90, 157, 196], 'presence': [96, 112], 'absence': [98], 'tryptophan': [100, 128], 'identified': [101], 'tryptophanase': [103], '(tna)': [105], 'showed': [107], 'strong': [108], 'induction': [109, 126], 'tryptophan.': [114], 'qRT-PCR': [115], 'revealed': [116], 'very': [118, 150], 'fast': [119], 'response': [120], 'an': [122], 'up': [123], '100-fold': [125], 'after': [127], 'addition.': [129], 'This': [130], 'result': [131], 'has': [132], 'been': [133], 'confirmed': [134], 'using': [135], 'three': [136], 'independent': [137], 'reporter': [138], 'genes': [139], '(cct1,': [140], 'pyrE2': [141], 'bgaH).': [143], 'Vectors': [144], 'containing': [145], 'promoter': [147, 171], 'will': [148], 'useful': [151], 'investigating': [153], 'function': [155, 194], 'potentially': [160], 'other': [162], 'halophilic': [163], 'archaea.': [164], 'To': [165], 'demonstrate': [166], 'this,': [167], 'used': [169], 'follow': [173], 'consequences': [175], 'depletion': [177], 'chaperonin': [181], 'protein': [182], 'CCT1,': [183], 'determine': [186], 'ability': [188], 'heterologous': [190], 'CCT': [191], 'volcanii.': [197]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2000989488', 'counts_by_year': [{'year': 2024, 'cited_by_count': 6}, {'year': 2023, 'cited_by_count': 4}, {'year': 2022, 'cited_by_count': 2}, {'year': 2021, 'cited_by_count': 7}, {'year': 2020, 'cited_by_count': 6}, {'year': 2019, 'cited_by_count': 8}, {'year': 2018, 'cited_by_count': 4}, {'year': 2017, 'cited_by_count': 5}, {'year': 2016, 'cited_by_count': 4}, {'year': 2015, 'cited_by_count': 7}, {'year': 2014, 'cited_by_count': 2}, {'year': 2013, 'cited_by_count': 5}, {'year': 2012, 'cited_by_count': 4}], 'updated_date': '2024-09-11T00:55:52.162335', 'created_date': '2016-06-24'}