Potent Inhibition of Ca2+ Release-activated Ca2+ Channels and T-lymphocyte Activation by the Pyrazole Derivative BTP2

Name: Work Video:
Duration: 3 min 30 s
Christof Zitt; Bettina Strauß; Eva C. Schwarz; Nicola Spaeth; Georg Rast; Armin Hatzelmann; Markus Hoth
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'https://openalex.org/W2073321338', 'https://openalex.org/W2060217221', 'https://openalex.org/W2057167188', 'https://openalex.org/W2044815046', 'https://openalex.org/W1438272007', 'https://openalex.org/W131865840'], 'abstract_inverted_index': {'Ca2+': [0, 4, 6, 90, 187, 192, 195, 199, 206, 224, 233, 334, 338, 340, 424, 521, 526, 529, 533, 540, 558, 567, 668, 672, 684, 702, 732, 785, 792, 834, 1428, 1533, 1570, 1573, 1576, 1589, 1610, 2762, 2943, 3045, 3159, 3224, 3247, 3288, 3329, 3339, 3476, 3748, 3756, 3789, 3793, 3806, 3820, 3835, 3845, 3851, 3864, 3868, 3907, 3917, 3936, 3944, 3969, 3975, 3981, 4021, 4031, 4051, 4071, 4099, 4103, 4140, 4146, 4152, 4184, 4203, 4213, 4217, 4260, 4278, 4303, 4307, 4384, 4414, 4428, 4465, 4472, 4498, 4516, 4529, 4553, 4581, 4588, 4605, 4609, 4613, 4635], 'entry': [1, 234, 335, 568, 669, 3749, 3757, 3790, 3908, 4185, 4429, 4466, 4473], 'through': [2, 336, 670, 836, 3750, 3870], 'store-operated': [3, 337, 671, 1517, 1588, 3650, 3788, 3906, 3916, 4030, 4183], 'release-activated': [5, 339, 685], '(CRAC)': [7, 341], 'channels': [8, 169, 286, 294, 342, 503, 620, 628, 734, 797, 838, 877, 1000, 1070, 1552, 1580, 1606, 1622, 3752, 3872], 'is': [9, 226, 277, 343, 560, 611, 737, 881, 1062, 1512, 1545, 1613, 3753, 3763, 4027, 4249, 4452, 4460], 'essential': [10, 344, 739], 'for': [11, 94, 120, 186, 345, 428, 454, 520, 851, 878, 1604, 1676, 1726, 1759, 1782, 1934, 2310, 2352, 2455, 2489, 2624, 2692, 2737, 2769, 2793, 3033, 3038, 3077, 3197, 3226, 3241, 3292, 3426, 3444, 3492, 3504, 3680, 3765, 3897, 4122, 4316, 4492, 4630], 'T-cell': [12, 244, 315, 346, 578, 649, 688, 879, 2040, 2095, 2265, 4482, 4493], 'activation': [13, 347, 743, 767, 3648, 3767, 4426], 'and': [14, 77, 108, 153, 201, 217, 246, 263, 265, 268, 281, 348, 411, 442, 487, 535, 551, 580, 597, 599, 602, 615, 775, 781, 891, 913, 1076, 1101, 1279, 1284, 1558, 1578, 1596, 1607, 1617, 1630, 1780, 1809, 1884, 1902, 1929, 1978, 2019, 2034, 2055, 2254, 2267, 2413, 2467, 2492, 2530, 2621, 2637, 2668, 2698, 2733, 2796, 2844, 2872, 2906, 2959, 3005, 3068, 3110, 3155, 3232, 3256, 3271, 3447, 3529, 3552, 3582, 3612, 3691, 3718, 3761, 3768, 3798, 3942, 3979, 3995, 4009, 4035, 4060, 4102, 4114, 4150, 4174, 4189, 4201, 4305, 4358, 4379, 4404, 4424, 4568, 4611], 'proliferation.': [15, 349, 3769], 'Recently,': [16, 350, 1282], 'it': [17, 116, 351, 450, 3762, 4026], 'has': [18, 352, 3783], 'been': [19, 353, 1002], 'shown': [20, 354, 4250, 4322], 'that': [21, 164, 355, 498, 1407, 1502, 1543, 2503, 3774, 4029, 4180, 4381, 4441, 4505], '3,5-bistrifluoromethyl': [22, 356, 3776], 'pyrazole': [23, 357, 1405, 1506, 3777], '(BTP)': [24, 358], 'derivatives': [25, 359, 1406, 1507], 'are': [26, 360, 1280, 3725, 4000, 4038, 4079, 4209, 4222, 4321, 4435], 'specific': [27, 282, 361, 616, 1616], 'inhibitors': [28, 362, 1067], 'of': [29, 89, 175, 223, 228, 270, 284, 292, 310, 331, 363, 423, 509, 557, 562, 604, 618, 626, 644, 665, 768, 847, 875, 887, 918, 1068, 1413, 1426, 1504, 1516, 1528, 1550, 1584, 1620, 1656, 1709, 1712, 1743, 1794, 1847, 1910, 1913, 1926, 1955, 1965, 1983, 1991, 1997, 2048, 2101, 2139, 2168, 2251, 2263, 2332, 2374, 2383, 2395, 2404, 2407, 2430, 2436, 2439, 2474, 2482, 2512, 2542, 2565, 2570, 2579, 2589, 2683, 2706, 2713, 2725, 2757, 2815, 2884, 2903, 2915, 3139, 3152, 3158, 3172, 3208, 3268, 3275, 3320, 3336, 3353, 3369, 3373, 3393, 3405, 3421, 3429, 3449, 3472, 3475, 3490, 3618, 3640, 3649, 3664, 3685, 3800, 3805, 3826, 3844, 3882, 3890, 3905, 3915, 3955, 3966, 3988, 4003, 4044, 4116, 4137, 4164, 4267, 4285, 4297, 4337, 4345, 4369, 4374, 4383, 4398, 4413, 4427, 4444, 4457, 4464, 4480, 4501, 4528, 4542, 4545, 4559, 4580, 4585, 4602, 4633], 'Ca2+-dependent': [30, 258, 298, 364, 592, 632, 1414, 1593], 'transcriptional': [31, 365], 'activity': [32, 313, 366, 647, 880, 890], 'in': [33, 170, 189, 208, 214, 218, 287, 305, 367, 504, 523, 542, 548, 552, 621, 639, 735, 798, 922, 1004, 1007, 1275, 1430, 1519, 1535, 1554, 1581, 1623, 1631, 1686, 1698, 1762, 1837, 1962, 2016, 2021, 2044, 2083, 2108, 2152, 2284, 2339, 2379, 2386, 2392, 2411, 2415, 2508, 2522, 2539, 2576, 2658, 2679, 2686, 2746, 2778, 2912, 2917, 2971, 2990, 3001, 3055, 3086, 3112, 3169, 3244, 3295, 3310, 3434, 3457, 3481, 3498, 3517, 3566, 3759, 3791, 3795, 3817, 3837, 3878, 3886, 3923, 3962, 4020, 4033, 4055, 4077, 4087, 4156, 4168, 4186, 4192, 4219, 4226, 4246, 4251, 4263, 4376, 4416, 4437, 4447, 4467, 4474, 4509, 4556, 4598, 4615, 4637, 4647], 'T-cells': [34, 171, 368, 505, 1431, 1536, 1560, 1627, 1856, 2919, 3052, 4037, 4090, 4158, 4170, 4191, 4406, 4451], '(Trevillyan,': [35, 369], 'J.': [36, 72, 82, 112, 149, 370, 406, 416, 446, 483, 857, 902, 941, 982, 1126, 1140, 1167, 1185, 1211, 1311, 1344, 1372, 1378, 1387, 1447, 1477, 1490, 1824, 2068, 2124, 2205, 2238, 2929], 'M.,': [37, 144, 150, 371, 478, 484], 'Chiou,': [38, 127, 372, 461], 'X.': [39, 373, 1307, 2201], 'G.,': [40, 374], 'Chen,': [41, 375], 'Y.': [42, 376, 1433], 'W.,': [43, 70, 76, 147, 377, 404, 410, 481], 'Ballaron,': [44, 130, 378, 464], 'S.': [45, 79, 155, 379, 413, 489, 824, 980, 1146, 1309, 1439, 2203], 'J.,': [46, 59, 62, 67, 97, 140, 380, 393, 396, 401, 431, 474], 'Sheets,': [47, 132, 381, 466], 'M.': [48, 51, 125, 133, 382, 385, 459, 467, 803, 897, 929, 959, 965, 1032, 1106, 1148, 1164, 1329, 1335, 1451, 2063, 2119, 2223, 2229, 2926], 'P.,': [49, 134, 383, 468], 'Smith,': [50, 124, 384, 458], 'L.,': [52, 126, 386, 460], 'Wiedeman,': [53, 387], 'P.': [54, 388, 1124], 'E.,': [55, 136, 389, 470], 'Warrior,': [56, 137, 390, 471], 'U.,': [57, 138, 391, 472], 'Wilkins,': [58, 139, 392, 473], 'Gubbins,': [60, 135, 394, 469], 'E.': [61, 395, 1321, 1443, 2215], 'Gagne,': [63, 397], 'G.': [64, 69, 128, 146, 398, 403, 462, 480, 2924], 'D.,': [65, 399], 'Fagerland,': [66, 400], 'Carter,': [68, 145, 402, 479], 'Luly,': [71, 405], 'R.,': [73, 103, 407, 437], 'Mollison,': [74, 151, 408, 485], 'K.': [75, 409, 1152, 1180, 1208, 1457, 1479], 'Djuric,': [78, 154, 412, 488], 'W.': [80, 156, 414, 490], '(2001)': [81, 415], 'Biol.': [83, 417, 858, 904, 942, 1186, 1212, 1388, 2070, 2126, 2930], 'Chem.': [84, 418, 859, 943, 1187, 1213, 1346, 1389, 2240, 2931], '276,': [85, 419], '48118-48126).': [86, 420], 'Whereas': [87, 421], 'inhibition': [88, 291, 422, 625, 1425, 3363, 3388, 3474, 3965, 4136, 4296, 4336, 4354, 4382, 4446, 4632], 'signals': [91, 188, 207, 225, 425, 522, 541, 559, 1429, 1534, 3477, 3794, 4022, 4032, 4073, 4214, 4385, 4582], 'was': [92, 117, 235, 426, 451, 569, 1696, 1746, 1906, 2000, 2135, 2155, 2170, 2377, 2424, 2582, 2635, 2639, 2709, 2728, 2744, 2888, 2967, 2984, 3031, 3331, 3364, 3381, 3389, 3455, 3462, 3745, 3811, 4053, 4234, 4339, 4348, 4390, 4484, 4490, 4535, 4573, 4591], 'reported': [93, 427], 'BTP2': [95, 165, 204, 255, 276, 318, 429, 499, 538, 589, 610, 652, 1509, 1544, 1591, 1612, 3473, 3491, 3779, 3801, 3896, 3960, 4061, 4121, 4181, 4225, 4270, 4318, 4338, 4375, 4389, 4521, 4562, 4572, 4631], '(Ishikawa,': [96, 430], 'Ohga,': [98, 432], 'K.,': [99, 152, 433, 486], 'Yoshino,': [100, 434], 'T.,': [101, 435], 'Takezawa,': [102, 436], 'Ichikawa,': [104, 438], 'A.,': [105, 439], 'Kubota,': [106, 440], 'H.,': [107, 441], 'Yamada,': [109, 443], 'T.': [110, 444, 1144, 1150, 1317, 1481, 1489, 2211], '(2003)': [111, 445], 'Immunol.': [113, 159, 447, 493, 748, 990, 1018, 1461, 1491], '170,': [114, 448], '4441-4449),': [115, 449], 'not': [118, 180, 452, 514, 1423, 1525, 1567, 2159, 4024, 4068, 4210, 4216, 4362, 4523, 4539], 'found': [119, 453, 1603, 4380], 'BTP3': [121, 455], '(Chen,': [122, 456], 'Y.,': [123, 457], 'X.,': [129, 463], 'S.,': [131, 465], 'Surowy,': [141, 475], 'C.,': [142, 476], 'Nakane,': [143, 477], 'Trevillyan,': [148, 482], '(2002)': [157, 491], 'Cell.': [158, 492, 1460], '220,': [160, 494], '134-142).': [161, 495], 'We': [162, 496, 1541, 4178], 'show': [163, 497, 1542], 'specifically': [166, 500, 1409, 2160], 'inhibits': [167, 205, 501, 539, 1592, 4182], 'CRAC': [168, 285, 293, 311, 332, 502, 619, 627, 645, 666, 678, 796, 876, 888, 919, 999, 1069, 1083, 1088, 1551, 1605, 1621, 3751, 3871], 'with': [172, 182, 239, 250, 272, 301, 506, 516, 573, 584, 606, 635, 925, 1410, 1569, 1599, 1653, 1734, 1798, 1975, 2002, 2026, 2052, 2089, 2317, 2327, 2368, 2547, 2617, 2772, 2781, 2787, 2822, 2847, 2864, 2964, 2976, 3047, 3060, 3091, 3190, 3222, 3252, 3264, 3487, 3534, 3557, 3592, 3893, 3959, 4236, 4351, 4513, 4525, 4550, 4624], 'an': [173, 507, 738, 1950, 2816, 2911, 3265, 3272, 3593, 4352, 4461], 'IC50': [174, 274, 303, 508, 608, 637, 1273, 3986, 4162, 4628], '∼10': [176, 510], 'nm.': [177, 511, 2759, 3277], 'It': [178, 512, 1565], 'does': [179, 513, 1566], 'interfere': [181, 515, 1408, 1568, 4524], 'other': [183, 517], 'mechanisms': [184, 518, 1530], 'important': [185, 519], 'T-cells,': [190, 524, 736, 1582, 1875], 'including': [191, 525, 766, 1012, 1094, 2420, 3703], 'pumps,': [193, 527, 1571], 'mitochondrial': [194, 528, 1572], 'signaling,': [196, 530, 1574], 'endoplasmic': [197, 531, 788, 3809, 4207], 'reticulum': [198, 532, 789, 3810, 4208], 'release,': [200, 534, 1577], 'K+': [202, 536, 1579, 2993], 'channels.': [203, 333, 537, 667], 'peripheral': [209, 543, 694, 710, 1555], 'blood': [210, 544, 695, 711, 1556, 1629, 1644, 1661, 1702, 1791, 2487], 'T-lymphocytes': [211, 271, 306, 545, 605, 640, 1013, 1557, 1786], '(in': [212, 546, 2947, 3510, 3540], 'particular': [213, 547], 'CD4+': [215, 549, 1626, 1785, 1855, 1864, 1998, 2252, 2375, 3051, 4008, 4034, 4448], 'T-cells)': [216, 550], 'human': [219, 553, 1625, 2143, 4088], 'Jurkat': [220, 554, 1520, 1559, 1632, 2039, 2094, 2144, 2150, 2918, 3082, 3482, 4089, 4157, 4169, 4190, 4220, 4405, 4417, 4450, 4639], 'T-cells.': [221, 555, 1521, 1633, 4468], 'Inhibition': [222, 556], 'independent': [227, 561], 'the': [229, 243, 278, 308, 328, 563, 577, 612, 642, 662, 760, 777, 787, 799, 845, 852, 884, 915, 1095, 1276, 1411, 1471, 1505, 1529, 1608, 1614, 1642, 1651, 1707, 1744, 1863, 1939, 2003, 2008, 2097, 2102, 2109, 2140, 2142, 2153, 2173, 2260, 2264, 2268, 2276, 2281, 2285, 2359, 2402, 2408, 2434, 2479, 2504, 2509, 2513, 2536, 2543, 2563, 2566, 2585, 2642, 2652, 2704, 2714, 2813, 2848, 2891, 2901, 2972, 2991, 3016, 3025, 3048, 3206, 3209, 3223, 3245, 3346, 3367, 3370, 3385, 3387, 3395, 3419, 3452, 3458, 3469, 3567, 3658, 3665, 3692, 3754, 3772, 3775, 3784, 3796, 3808, 3824, 3841, 3854, 3858, 3874, 3891, 3913, 3926, 3935, 3943, 3967, 3974, 3980, 3989, 4049, 4082, 4129, 4138, 4145, 4151, 4165, 4193, 4206, 4227, 4237, 4252, 4258, 4264, 4268, 4298, 4302, 4306, 4343, 4346, 4367, 4370, 4394, 4445, 4481, 4526, 4543, 4557, 4603, 4608, 4612, 4616, 4625, 4627], 'stimulation': [230, 238, 249, 564, 572, 583, 2373, 3480, 4076, 4479], 'method': [231, 565], 'as': [232, 295, 297, 566, 629, 631, 677, 1602, 1817, 2057, 2673, 2920, 3281, 3366, 3391, 3727, 3733, 3884, 3930, 4244, 4323, 4645], 'blocked': [236, 570], 'following': [237, 248, 571, 582, 791, 1417, 2172, 2562, 3478, 4074, 4419, 4476, 4583], 'anti-CD3,': [240, 574], 'which': [241, 575, 771, 843, 1527, 1585, 3036, 4595], 'activates': [242, 576, 839], 'receptor,': [245, 579], 'also': [247, 256, 581, 590, 674, 4340, 4485], 'thapsigargin': [251, 585], 'or': [252, 586, 1136, 2280, 2557, 2830, 3145, 3313, 3398, 3731, 3740, 4048, 4277, 4449], 'inositol': [253, 587, 691], '1,4,5-trisphosphate.': [254, 588], 'inhibited': [257, 591, 4223, 4486], 'gene': [259, 593, 892, 1594], 'expression': [260, 594, 846, 893, 1412, 1595], '(interleukins': [261, 595], '2': [262, 596, 2388, 2955, 3012, 3198, 3546, 4396, 4455], '5': [264, 598, 2885, 2960, 3140, 3170, 3553, 3660, 3950], 'interferon': [266, 600], 'γ)': [267, 601], 'proliferation': [269, 603, 1598, 1783, 2277], 'similar': [273, 302, 607, 636, 1600], 'values.': [275, 609], 'first': [279, 613, 1615, 3659, 4395], 'potent': [280, 614, 1097, 1514, 1548, 1618], 'inhibitor': [283, 617, 1515, 1549, 1619, 2409], 'primary': [288, 622, 1624], 'T-lymphocytes.': [289, 623], 'The': [290, 624, 873, 1693, 1994, 2372, 2854, 3362, 3441, 3536, 3985, 4161, 4254], 'well': [296, 630, 2331], 'signal': [299, 633], 'transduction': [300, 634], 'values': [304, 638, 1274, 2902, 3372, 3397, 3400, 3412, 3987, 4163, 4629], 'emphasizes': [307, 641], 'importance': [309, 643, 874], 'channel': [312, 646, 889, 920, 1084, 1089], 'during': [314, 648, 741, 2584, 3464], 'activation.': [316, 650, 4494], 'Furthermore,': [317, 651], 'could': [319, 653, 1071, 1422, 1500], 'prove': [320, 654], 'to': [321, 325, 655, 659, 676, 1081, 1532, 1757, 1843, 1921, 1949, 1980, 2342, 2354, 2426, 2478, 2498, 2501, 2718, 2730, 2802, 2804, 3023, 3079, 3134, 3205, 3238, 3345, 3375, 3383, 3407, 3633, 3786, 3861, 3901, 4064, 4118, 4128, 4257, 4283, 4507, 4548], 'be': [322, 656, 1078, 2499], 'a': [323, 657, 1513, 1546, 1668, 1844, 1923, 1981, 2333, 2380, 2393, 2427, 2597, 2680, 2747, 2823, 2831, 2865, 3039, 3136, 3253, 3615, 3637, 3681, 3738, 3831, 3902, 3963, 4001, 4017, 4134, 4241, 4514, 4536, 4551, 4578, 4599], 'tool': [324, 658], 'finally': [326, 660], 'unmask': [327, 661], 'molecular': [329, 663, 1059, 1074], 'identity': [330, 664], 'channels,': [673], 'referred': [675], '1The': [679], 'abbreviations': [680], 'used': [681, 1781, 3022, 3032, 3382, 4491], 'are:': [682], 'CRAC,': [683], 'Ca2+;': [686], 'TCR,': [687, 1901], 'receptor;': [689], 'InsP3,': [690], '1,4,5-trisphosphate;': [692], 'PBL,': [693], 'lymphocyte;': [696], 'TG,': [697], 'thapsigargin;': [698], '[Ca2+]i,': [699], 'free': [700, 3303, 3341], 'cytosolic': [701], 'concentration;': [703], 'HBSS,': [704], "Hanks'": [705], 'balanced': [706], 'salt': [707, 2716], 'solution;': [708], 'PBMCs,': [709], 'mononuclear': [712, 1662], 'cells;': [713], 'RT,': [714], 'room': [715, 3239], 'temperature;': [716], 'PBS,': [717], 'phosphate-buffered': [718], 'saline;': [719], 'FCS,': [720, 1967, 2028, 3062], 'fetal': [721], 'calf': [722], 'serum;': [723], 'mAb,': [724], 'monoclonal': [725, 2446], 'antibody;': [726], 'IL-2,': [727, 2555], 'interleukin-2;': [728], 'PMCA,': [729], 'plasma': [730, 800, 3855, 3875], 'membrane': [731, 801, 3876], 'ATPase.': [733], 'step': [740], 'T-lymphocyte': [742], '(1Lewis': [744, 1014], 'R.S.': [745, 818, 901, 1015, 1166, 2067, 2123], 'Annu.': [746, 1016], 'Rev.': [747, 1017, 1049], '2001;': [749, 860, 991, 1019, 1169, 1390, 1828], '19:': [750, 1020], '497-521Crossref': [751, 1021], 'PubMed': [752, 810, 829, 868, 908, 950, 976, 994, 1022, 1039, 1053, 1114, 1131, 1158, 1172, 1196, 1222, 1239, 1267, 1350, 1398, 1465, 1495, 2074, 2130, 2244, 2938], 'Scopus': [753, 811, 830, 869, 909, 995, 1023, 1040, 1054, 1115, 1132, 1159, 1173, 1197, 1223, 1240, 1268, 1351, 1399, 1466, 1496, 2075, 2131, 2245, 2939], '(705)': [754, 1024], 'Google': [755, 813, 832, 871, 911, 951, 977, 997, 1025, 1042, 1056, 1117, 1134, 1161, 1175, 1199, 1225, 1242, 1270, 1353, 1401, 1468, 1498, 1831, 2077, 2133, 2247, 2941], 'Scholar).': [756, 833, 872, 998, 1057, 1469, 1832, 2078, 2248, 2942], 'Following': [757], 'antigen': [758], 'presentation,': [759], 'TCR': [761, 1418], 'initiates': [762], 'several': [763, 840], 'signaling': [764], 'cascades,': [765], 'phospholipase': [769], 'Cγ,': [770], 'cleaves': [772], 'phosphatidylinositol': [773], '4,5-bisphosphate': [774], 'generates': [776], 'second': [778], 'messengers': [779], 'InsP3': [780, 3513], 'diacylglycerol.': [782], 'InsP': [783], 'releases': [784], 'from': [786, 1628, 1637, 1641, 1789, 2163, 2485, 2535, 2647, 2890, 2910, 3015, 3630, 3636, 3807, 3840, 3846, 4205, 4643], 'and,': [790], 'depletion,': [793], 'subsequently': [794], 'opens': [795], '(2Hoth': [802], 'Penner': [804, 1046, 1107], 'R.': [805, 984, 1028, 1047, 1108, 1202, 1483], 'Nature.': [806], '1992;': [807], '355:': [808], '353-356Crossref': [809], '(1491)': [812], 'Scholar,': [814, 952, 978, 1026, 1043, 1118, 1162, 1176, 1200, 1226, 1243, 1354], '3Zweifach': [815], 'A.': [816, 825, 936, 971, 988, 1291, 1485, 1821, 1904, 2185], 'Lewis': [817, 900, 1165, 2066, 2122], 'Proc.': [819], 'Natl.': [820], 'Acad.': [821], 'Sci.': [822], 'U.': [823, 1339, 1370, 1445, 2233], '1993;': [826, 1036], '90:': [827], '6295-6299Crossref': [828], '(695)': [831], 'influx': [835, 1518, 3330, 3869, 3928, 3970, 4141, 4300, 4606, 4636], 'those': [837], 'transcription': [841], 'factors,': [842], 'regulate': [844], 'cytokine': [848, 1415, 2282, 2475, 2483, 2493, 2505], 'genes': [849], 'critical': [850], 'immune': [853], 'response': [854], '(4Crabtree': [855], 'G.R.': [856, 899, 2065, 2121], '276:': [861, 1391], '2313-2316Abstract': [862], 'Full': [863, 865, 947, 1191, 1193, 1217, 1219, 1393, 1395, 2935], 'Text': [864, 866, 948, 1192, 1194, 1218, 1220, 1394, 1396, 2936], 'PDF': [867, 949, 1195, 1221, 1397, 2937], '(379)': [870], 'evident': [882], 'by': [883, 914, 1539, 1649, 1667, 1706, 1748, 1755, 1802, 1859, 1888, 1908, 1944, 2112, 2176, 2325, 2401, 2711, 2969, 2986, 3102, 3211, 3316, 3333, 3349, 3598, 3626, 3670, 3813, 3850, 3932, 3939, 4040, 4159, 4224, 4280, 4309, 4386, 4422, 4487], 'close': [885], 'correlation': [886], '(5Fanger': [894, 2060, 2116], 'C.M.': [895, 1245, 2061, 2117], 'Hoth': [896, 1031, 1105, 2062, 2118], 'Crabtree': [898, 2064, 2120], 'Cell': [903, 1262, 2069, 2125, 2761], '1995;': [905, 973, 2071, 2127], '131:': [906, 2072, 2128], '655-667Crossref': [907, 2073, 2129], '(164)': [910, 2076, 2132], 'Scholar)': [912, 1135, 1402, 1499, 2134], 'devastating': [916], 'consequences': [917], 'impairment': [921], 'some': [923, 2138], 'patients': [924], 'severe-combined': [926], 'immunodeficiencies': [927], '(6Partiseti': [928], 'Le': [930, 2303], 'Deist': [931, 954], 'F.': [932, 955], 'Hivroz': [933, 956], 'C.': [934, 957, 961, 1030, 1210, 1449, 1823], 'Fischer': [935, 970], 'Korn': [937], 'H.': [938, 1204, 1293, 1487, 2187], 'Choquet': [939, 968], 'D.': [940, 969, 1104, 1319, 2213], '1994;': [944, 1111, 1128], '269:': [945], '32327-32335Abstract': [946], '7Le': [953], 'Partiseti': [958], 'Thomas': [960], 'Buc': [962], 'H.A.': [963], 'Oleastro': [964], 'Belohradsky': [966], 'B.': [967], 'Blood.': [972], '85:': [974], '1053-1062Crossref': [975], '8Feske': [979], 'Giltnane': [981], 'Dolmetsch': [983], 'Staudt': [985], 'L.M.': [986], 'Rao': [987], 'Nat.': [989], '2:': [992], '316-324Crossref': [993], '(500)': [996], 'have': [1001, 1272, 1403], 'characterized': [1003], 'great': [1005], 'detail': [1006, 4378], 'many': [1008], 'different': [1009, 2486, 4004], 'cell': [1010, 1597, 1845, 2104, 2660, 3137, 3153, 3506, 3570, 3583, 3622, 3667, 3766, 4232], 'types,': [1011], '9Penner': [1027], 'Fasolato': [1029], 'Curr.': [1033], 'Opin.': [1034], 'Neurobiol.': [1035], '3:': [1037], '368-374Crossref': [1038], '(126)': [1041], '10Parekh': [1044], 'A.B.': [1045, 1253], 'Physiol.': [1048, 1168], '1997;': [1050], '77:': [1051], '901-930Crossref': [1052], '(1291)': [1055], 'Their': [1058], 'identity,': [1060], 'however,': [1061, 1524, 4133, 4342], 'still': [1063], 'largely': [1064], 'unknown.': [1065], 'Specific': [1066], 'facilitate': [1072], 'their': [1073], 'identification': [1075], 'would': [1077], 'excellent': [1079], 'tools': [1080], 'study': [1082], 'function.': [1085], 'Unfortunately,': [1086], 'all': [1087, 1583, 2418, 2531, 3034, 4014], 'blockers': [1090], 'described': [1091, 1404, 1818, 2058, 2921, 4245], 'so': [1092], 'far,': [1093], 'most': [1096, 2093], 'ones,': [1098], 'SK&F': [1099], '96365': [1100], 'econazole': [1102], '(11Franzius': [1103], 'Pflugers': [1109], 'Arch.': [1110], '428:': [1112], '433-438Crossref': [1113], '(159)': [1116], '12Chung': [1119], 'S.C.': [1120], 'McDonald': [1121], 'T.V.': [1122], 'Gardner': [1123], 'Br.': [1125], 'Pharmacol.': [1127, 1154, 1235, 1825], '113:': [1129], '861-868Crossref': [1130], '(106)': [1133], '2-aminoethyldiphenyl': [1137], 'borate': [1138], '(13Wu': [1139], 'Kamimura': [1141], 'N.': [1142], 'Takeo': [1143], 'Suga': [1145], 'Wakui': [1147], 'Maruyama': [1149], 'Mikoshiba': [1151], 'Mol.': [1153, 1234], '2000;': [1155, 1347, 2241], '58:': [1156], '1368-1374Crossref': [1157], '(57)': [1160], '14Prakriya': [1163], '536:': [1170], '3-19Crossref': [1171], '(428)': [1174], '15Ma': [1177], 'H.T.': [1178], 'Venkatachalam': [1179], 'Parys': [1181], 'J.B.': [1182], 'Gill': [1183], 'D.L.': [1184], '2002;': [1188, 1214, 1462], '277:': [1189, 1215], '6915-6922Abstract': [1190], '(150)': [1198], '16Schindl': [1201], 'Kahr': [1203], 'Graz': [1205], 'I.': [1206], 'Groschner': [1207], 'Romanin': [1209], '26950-26958Abstract': [1216], '(81)': [1224], '17Braun': [1227], 'F.J.': [1228], 'Aziz': [1229], 'O.': [1230], 'Putney': [1231], 'Jr.,': [1232], 'J.W.': [1233], '2003;': [1236, 1264, 1492], '63:': [1237], '1304-1311Crossref': [1238], '(47)': [1241], '18Peppiatt': [1244], 'Collins': [1246], 'T.J.': [1247], 'Mackenzie': [1248], 'L.': [1249], 'Conway': [1250], 'S.J.': [1251, 1362], 'Holmes': [1252], 'Bootman': [1254], 'M.D.': [1255], 'Berridge': [1256], 'M.J.': [1257], 'Seo': [1258], 'J.T.': [1259], 'Roderick': [1260], 'H.L.': [1261], 'Calcium.': [1263], '34:': [1265], '97-108Crossref': [1266], '(234)': [1269], 'Scholar),': [1271], 'micromolar': [1277], 'range': [1278, 2511], 'nonspecific.': [1281], 'Djuric': [1283, 1385, 1458, 2177], 'colleagues': [1285], '(19Djuric': [1286, 2180], 'S.W.': [1287, 1386, 1459, 2181], 'BaMaung': [1288, 2182], 'N.Y.': [1289, 2183], 'Basha': [1290, 2184], 'Liu': [1292, 2186], 'Luly': [1294, 1381, 2188], 'J.R.': [1295, 1382, 2189], 'Madar': [1296, 2190], 'D.J.': [1297, 2191], 'Sciotti': [1298, 2192], 'R.J.': [1299, 2193], 'Tu': [1300, 2194], 'N.P.': [1301, 2195], 'Wagenaar': [1302, 2196], 'F.L.': [1303, 2197], 'Wiedeman': [1304, 1367, 2198], 'P.E.': [1305, 1368, 2199], 'Zhou': [1306, 2200], 'Ballaron': [1308, 1361, 1438, 2202], 'Bauch': [1310, 2204], 'Chen': [1312, 1359, 2206], 'Y.W.': [1313, 1360, 2207], 'Chiou': [1314, 1357, 1436, 2208], 'X.G.': [1315, 1358, 2209], 'Fey': [1316, 2210], 'Gauvin': [1318, 2212], 'Gubbins': [1320, 1373, 1442, 2214], 'Hsieh': [1322, 2216], 'G.C.': [1323, 2217], 'Marsh': [1324, 2218], 'K.C.': [1325, 2219], 'Mollison': [1326, 1383, 1456, 2220], 'K.W.': [1327, 1384, 2221], 'Pong': [1328, 2222], 'Shaughnessy': [1330, 2224], 'T.K.': [1331, 2225], 'Sheets': [1332, 1363, 1440, 2226], 'M.P.': [1333, 1364, 1441, 2227], 'Smith': [1334, 1365, 1434, 2228], 'Trevillyan': [1336, 1454, 2230], 'J.M.': [1337, 1356, 1455, 2231], 'Warrior': [1338, 1369, 1444, 2232], 'Wegner': [1340, 2234], 'C.D.': [1341, 2235], 'Carter': [1342, 1379, 1452, 2236], 'G.W.': [1343, 1380, 1453, 2237], 'Med.': [1345, 2239], '43:': [1348, 2242], '2975-2981Crossref': [1349, 2243], '(114)': [1352, 2246], '20Trevillyan': [1355], 'M.L.': [1366, 1435], 'Wilkins': [1371, 1446], 'E.J.': [1374], 'Gagne': [1375], 'G.D.': [1376], 'Fagerland': [1377], '48118-48126Abstract': [1392], '(90)': [1400], 'production': [1416], 'stimulation,': [1419], 'but': [1420, 4215, 4538], 'they': [1421], 'detect': [1424], 'TCR-dependent': [1427], '(21Chen': [1432], 'G.X.': [1437], 'Surowy': [1448], 'Nakane': [1450], '220:': [1463], '134-142Crossref': [1464], '(26)': [1467], 'On': [1470], 'contrary,': [1472], 'Ishikawa': [1473], 'et': [1474, 2114, 2178], 'al.': [1475, 2115, 2179], '(22Ishikawa': [1476], 'Ohga': [1478], 'Yoshino': [1480], 'Takezawa': [1482], 'Ichikawa': [1484], 'Kubota': [1486], 'Yamada': [1488], '170:': [1493], '4441-4449Crossref': [1494], '(231)': [1497], 'demonstrate': [1501], 'one': [1503], 'named': [1508], '(or': [1510], 'YM-58483)': [1511], 'They': [1522], 'did,': [1523], 'discriminate': [1526], 'contributing': [1531], 'were': [1537, 1635, 1647, 1665, 1704, 1732, 1752, 1778, 1787, 1815, 1835, 1857, 1886, 1941, 1973, 2014, 2042, 2080, 2162, 2257, 2291, 2308, 2347, 2365, 2398, 2453, 2464, 2507, 2520, 2533, 2545, 2594, 2613, 2655, 2671, 2677, 2690, 2735, 2764, 2800, 2838, 2862, 2880, 2908, 3021, 3053, 3084, 3100, 3177, 3188, 3203, 3220, 3236, 3250, 3279, 3290, 3413, 3424, 3485, 3496, 3561, 3585, 3607, 3624, 3655, 3678, 3697, 3802, 3921, 3992, 4085, 4107, 4171, 4313, 4641], 'affected': [1538], 'BTP2.': [1540, 3440, 4041, 4160, 4310], 'very': [1547, 3832, 4080], 'themselves': [1553], '(IC50': [1561], 'around': [1562], '10': [1563, 1718, 1739, 1911, 1935, 2405, 2437, 2577, 2782, 2794, 2957, 3107, 3118, 3523, 3527, 3530, 3550], 'nm).': [1564], 'ER': [1575], 'greatly': [1586], 'influence': [1587], 'signals.': [1590, 1611], 'potency': [1601], 'corresponding': [1609], 'Cells—PBLs': [1634], 'purified': [1636, 1754], 'leukocyte': [1638], 'reduction': [1639, 2712, 3914, 4019, 4579, 4601], 'filters': [1640], 'local': [1643], 'bank.': [1645], 'Cells': [1646, 1751, 1972, 2079, 2253, 2593, 2799, 2837, 3099, 3202, 3219], 'collected': [1648, 1779, 3101, 3280], 'back-flushing': [1650], 'filter': [1652], '120': [1654], 'ml': [1655, 1688, 1711, 3171], 'HBSS': [1657, 1735], '(PAA,': [1658], '#15-009).': [1659], 'Peripheral': [1660], 'cells': [1663, 1703, 1745, 1851, 1920, 1961, 1987, 1999, 2013, 2256, 2376, 2452, 2708, 3083, 3423, 3450, 3484, 3892, 4015, 4221, 4312, 4418, 4504, 4546, 4640, 4652], '(PBMCs)': [1664], 'isolated': [1666, 1788], 'density': [1669, 1846, 1982, 2742, 3138], 'gradient': [1670], 'centrifugation': [1671, 1938, 3103, 3212], 'at': [1672, 1679, 1767, 1931, 2086, 2313, 2349, 2458, 2469, 2619, 2628, 2695, 2754, 2766, 2791, 2841, 2857, 2882, 3011, 3072, 3094, 3200, 3229, 3431, 3563, 3609, 3614, 4401, 4430], '450': [1673], '×': [1674, 1737, 1771, 1849, 1918, 1959, 1985, 2389, 3105, 3141, 3147, 3214], 'g': [1675], '30': [1677, 1963, 2770], 'min': [1678, 2771, 3228, 3243, 3294, 3402], 'RT': [1680, 2620, 2792], '(Ficoll-Paque™': [1681], 'plus,': [1682], 'Amersham': [1683], 'Biosciences,': [1684, 2302, 2664], '#17144002)': [1685], '50': [1687, 1927, 2328, 3156, 4519, 4560], 'Leucosep': [1689], 'tubes': [1690], '(Greiner,': [1691], '#227290).': [1692], 'PBMC': [1694], 'layer': [1695], 'washed': [1697, 1733, 1974, 2015, 2366, 2614, 2786], 'HBSS.': [1699], 'Remaining': [1700], 'red': [1701], 'removed': [1705], 'addition': [1708, 1909, 2403, 2435], '3': [1710, 1727, 2343, 2444], 'lysis': [1713], 'buffer': [1714], '(155': [1715], 'mm': [1716, 1719, 1722, 2783, 2978, 2982, 2988, 3000, 3116, 3119, 3122, 3125, 3128, 3132, 3175, 3300, 3308, 3323, 3325, 3338, 3356, 3358, 3515, 3819, 3863, 4587], 'NH4Cl,': [1717], 'KHCO3,': [1720], '0.1': [1721, 2035, 3069], 'EDTA,': [1723], 'pH': [1724, 3130], '7.3)': [1725], 'min.': [1728, 1936], 'After': [1729, 1937, 2272, 2433, 2701], 'lysis,': [1730], 'PBMCs': [1731], '(200': [1736], 'g,': [1738, 3106, 3215], 'min,': [1740, 1969, 2795, 3108, 3217], 'RT).': [1741, 3218], 'Viability': [1742], 'checked': [1747], 'trypan': [1749], 'blue.': [1750], 'further': [1753], 'adhering': [1756], 'plastic': [1758], '24': [1760, 2355, 3493, 3898, 3956, 4123], 'h': [1761, 2274, 2312, 2356, 2457, 2588, 2627, 2694, 3199, 3899, 3957, 4124, 4397, 4456], 'RPMI': [1763, 2022, 2049, 3056, 3087], '1640': [1764, 2023, 2050, 3057, 3088], 'complete': [1765, 3040, 4392, 4453], 'medium': [1766, 2024, 2046, 2287, 2780, 3058, 3089, 3436], '37': [1768, 2087, 2314, 2459, 2696, 3073, 3095, 3230, 3432, 4402], '°C': [1769, 1933, 2088, 2351, 2471, 2697, 2768, 3231, 3433, 3565], '(1.5': [1770], '106': [1772, 3142, 3148], 'cells/ml).': [1773], 'Non-adherent': [1774], 'cells,': [1775, 1879, 1883, 3386, 3483], 'mostly': [1776], 'PBLs,': [1777, 4130], 'assays.': [1784], 'venous': [1790], '(250': [1792], 'ml)': [1793], 'healthy': [1795], 'donors': [1796], 'anti-coagulated': [1797], 'citrate': [1799], '(0.3%': [1800], 'w/v)': [1801], 'successive': [1803], 'purification': [1804], 'steps.': [1805], 'Centrifugation,': [1806], 'percoll': [1807], 'gradient,': [1808], 'two': [1810, 4502], 'counter-current': [1811], 'centrifugal': [1812], 'elutriation': [1813], 'steps': [1814], 'performed': [1816, 1907, 2001, 2378, 2521, 3251, 3497, 3562, 4235], 'elsewhere': [1819], '(23Hatzelmann': [1820], 'Schudt': [1822], 'Exp.': [1826], 'Ther.': [1827], '297:': [1829], '267-279PubMed': [1830], 'Afterward,': [1833, 2345], 'lymphocytes': [1834, 4005], 're-suspended': [1836, 1979, 2020, 3111], 'PBS': [1838, 2340, 2369, 2523], 'containing': [1839, 2335, 2524, 3298, 3437], '2%': [1840], '(v/v)': [1841], 'FCS': [1842, 1977, 2018, 2054, 3093], '1': [1848, 1917, 1958, 1984, 2626, 2773, 2953, 2977, 2999, 3299, 3337, 3548, 3814, 3862, 4065, 4119, 4286, 4359, 4586], '107': [1850, 1919, 1960], 'per': [1852, 1916, 1957, 1988, 2330, 3179], '40': [1853, 3191], 'μl.': [1854], 'obtained': [1858, 3014], 'negative': [1860, 1995], 'selection': [1861, 1996], 'using': [1862, 1945, 2007, 2641, 2651, 2874, 3572, 3699], 'T-Cell': [1865], 'Isolation': [1866], 'Kit': [1867], 'II': [1868], '(Miltenyi': [1869], 'Biotec,': [1870], 'Bergisch': [1871], 'Gladbach,': [1872], 'Germany).': [1873, 2604, 3604], 'CD8+': [1874, 4011, 4036], 'γ/δT-cells,': [1876], 'monocytes,': [1877], 'dendritic': [1878], 'granulocytes,': [1880], 'B-cells,': [1881], 'erythroid': [1882], 'NK-cells': [1885], 'labeled': [1887, 1943], 'biotin-modified': [1889], 'antibodies': [1890], 'directed': [1891], 'against': [1892], 'CD8,': [1893], 'CD14,': [1894], 'CD16,': [1895], 'CD19,': [1896], 'CD36,': [1897], 'CD56,': [1898], 'CD123,': [1899], 'γ/δ': [1900], 'glycophorin': [1903], 'Labeling': [1905], 'μl': [1912, 1928, 1954, 1964, 1990, 2329, 2438, 2578, 2685, 2724, 3151, 3157, 3319, 3352], 'antibody': [1914, 1952, 1956, 2447], 'mixture': [1915], 'give': [1922, 3135], 'final': [1924, 2428], 'volume': [1925, 2382, 2394, 2682], 'incubation': [1930, 2702, 4399, 4432], '4': [1932, 1970, 2350, 2739, 3216], 'non-T-cells': [1940], 'magnetically': [1942], 'MACS': [1946], 'microbeads': [1947], 'coupled': [1948], 'anti-biotin': [1951], '(20': [1953], 'PBS/2%': [1966, 1976, 1992, 2017], '15': [1968, 3242], '°C).': [1971], '108': [1986], '500': [1989], 'FCS.': [1993], 'autoMACS': [2004], 'separation': [2005, 2009], 'unit': [2006], 'program': [2010], '"deplete."': [2011], 'Finally': [2012], 'supplemented': [2025, 2051, 3059, 3090], '10%': [2027, 2053, 3061, 3092], '1%': [2029, 3063], 'l-glutamine,': [2030, 3064], '100': [2031, 3065, 3894], 'units/ml': [2032, 3066], 'penicillin,': [2033, 3067], 'mg/ml': [2036, 3070], 'streptomycin.': [2037], 'Human': [2038], 'lines': [2041], 'grown': [2043], 'culture': [2045, 2286, 2580, 2590, 2661, 2779, 3435], 'consisting': [2047], 'penicillin-streptomycin': [2056], 'previously': [2059, 2922], 'continuously': [2081], 'maintained': [2082], 'log-phase': [2084], 'growth': [2085], '5%': [2090, 2461, 2699, 3075, 3097, 3233], 'CO2.': [2091, 2462, 2700, 3098, 3234], 'For': [2092, 2137, 2568, 3735, 4575], 'experiments,': [2096, 2141], 'diphtheria': [2098], 'toxin-resistant': [2099], 'version': [2100], 'parental': [2103], 'line': [2105], '(Jurkat': [2106], '(par)': [2107], 'figures)': [2110, 2154], 'generated': [2111], 'Fanger': [2113], 'used.': [2136, 2156, 2798, 3746], 'clone': [2145], 'E6.1': [2146, 4638], '(ATCC': [2147], 'number': [2148, 2705, 4544], 'TIB-152,': [2149], '(E6.1)': [2151], 'Reagents—All': [2157], 'chemicals': [2158], 'mentioned': [2161], 'Sigma': [2164], '(highest': [2165], 'grade).': [2166], 'Synthesis': [2167], 'BTP2—BTP2': [2169], 're-synthesized': [2171], 'procedure': [2174], 'provided': [2175], 'Functional': [2249], 'Assays': [2250], 'PBLs—CD4+': [2255], 'stimulated': [2258], 'via': [2259], 'CD3': [2261], 'chain': [2262], 'receptor': [2266, 4483], 'co-stimulatory': [2269], 'molecule': [2270], 'CD28.': [2271], '72': [2273, 2456], 'either': [2275, 3394], '([3H]thymidine': [2278], 'incorporation)': [2279], 'concentration': [2283, 2429, 3470], '(specific': [2288], 'enzyme': [2289], 'immunoassays)': [2290], 'measured.': [2292], '96-well': [2293, 2659, 3453], 'microtiter': [2294], 'plates': [2295, 2346, 2364, 2606, 2662, 2734, 3183], '(Microtest': [2296], 'Tissue': [2297], 'Culture': [2298], 'Plate,': [2299], 'Falcon,': [2300], 'BD': [2301], 'Pont': [2304], 'de': [2305], 'Claix,': [2306], 'France)': [2307, 2451, 2561], 'pre-coated': [2309, 3189], '2.5': [2311, 3127, 3146], '°C/5%': [2315], 'CO2': [2316, 3076], 'anti-CD3': [2318, 2338, 2360, 3006, 3380, 3479, 4489, 4508, 4549], 'mAb': [2319, 2441, 3007], '(Orthoclone': [2320], 'OKT-3,': [2321], 'Janssen-Cilag,': [2322], 'Neuss,': [2323], 'Germany)': [2324, 2612], 'incubating': [2326], 'solution': [2334, 2945, 2975, 3041, 3114, 3297, 3309, 3348, 3503, 3538, 3860, 3866, 4512, 4590], '60': [2336, 2629], 'μg/ml': [2337], '(corresponds': [2341], 'μg/well).': [2344], 'stored': [2348, 2468, 2790], 'up': [2353, 3078, 4063, 4117, 4282], 'without': [2357], 'removing': [2358], 'solution.': [2361, 2994, 3694, 3822], 'Before': [2362, 3181], 'use': [2363, 3182], 'twice': [2367], '(150': [2370], 'μl/well).': [2371], 'total': [2381, 2681], '200': [2384, 2684, 3438, 4387], 'μl/well': [2385, 2397, 2406, 3192], 'triplicates.': [2387, 2674], '105': [2390], 'cells/well': [2391], '180': [2396], 'applied': [2399, 3656, 3673], 'followed': [2400, 3669, 3849], '(solved': [2410], 'Me2SO': [2412, 2423], 'diluted': [2414], 'medium).': [2416], 'In': [2417, 3378, 3823, 4621], 'samples,': [2419, 2422], 'control': [2421, 4058, 4110, 4566], 'adjusted': [2425, 3237], '0.1%': [2431], '(v/v).': [2432], 'anti-CD28': [2440], '(final': [2442, 3302, 3340], 'concentration,': [2443], 'μg/ml,': [2445, 3009], 'CD28,': [2448], 'Immunotech,': [2449, 2559], 'Marseille,': [2450, 2560], 'incubated': [2454, 2691, 2736, 3425, 3486], '°C,': [2460, 3074, 3096], 'Supernatants': [2463], 'removed,': [2465], 'pooled,': [2466], '-20': [2470], 'until': [2472], 'determination': [2473, 2569], 'concentration.': [2476, 4319], 'Due': [2477], 'high': [2480, 2992], 'variability': [2481], 'levels': [2484, 2506, 4047, 4200], 'donors,': [2488, 4577], 'each': [2490, 2687, 2731, 3589, 4317], 'experiment': [2491, 4243], 'appropriate': [2494], 'dilution': [2495], 'factors': [2496], 'had': [2497, 4271], 'determined': [2500, 2534, 2710, 2909, 4642], 'guarantee': [2502], 'linear': [2510, 3933], 'enzyme-linked': [2514, 2550], 'immunosorbent': [2515, 2551], 'assay': [2516, 2552, 2654], 'standard': [2517, 3501], 'curve.': [2518], 'Dilutions': [2519], '3%': [2525], 'bovine': [2526], 'serum': [2527], 'albumin': [2528], '(w/v),': [2529], 'cytokines': [2532, 2544], 'pool': [2537], 'fraction': [2538], 'duplicate.': [2540], 'Measurements': [2541, 3495], 'done': [2546], 'commercially': [2548, 3700], 'available': [2549, 3701], 'kits': [2553], '(Jumbo': [2554], 'IFNγ,': [2556], 'IL-5,': [2558], 'instructions': [2564], 'manufacturer.': [2567], 'proliferation,': [2571], '[3H]thymidine': [2572], '(0.2': [2573], 'μCi/well,': [2574], 'added': [2575, 2729, 3178], 'medium)': [2581], 'present': [2583, 3463], 'last': [2586], '17': [2587], '(72': [2591], 'h).': [2592], 'harvested': [2595], 'on': [2596, 2812, 3451, 4274], 'Tomtec': [2598], 'Harvester': [2599], '96': [2600], '(Dunn': [2601], 'Labortechnik,': [2602], 'Asbach,': [2603], 'Filter': [2605], '(Uni': [2607], 'Filter-96,': [2608], 'GF/C;': [2609], 'Canberra-Packard,': [2610], 'Dreieich,': [2611], 'three': [2615, 3919], 'times': [2616], 'water': [2618], 'then': [2622], 'dried': [2623], 'about': [2625, 4442], '°C.': [2630], 'Scintillator': [2631], '(Microscint': [2632], 'O,': [2633], 'Canberra-Packard)': [2634], 'added,': [2636, 2785], 'radioactivity': [2638], 'measured': [2640, 2672, 2745], 'TopCount': [2643], 'microplate': [2644, 2750], 'scintillation': [2645], 'counter': [2646], 'Canberra-Packard.': [2648], 'Proliferation': [2649], 'experiments': [2650, 3249, 3560, 4500, 4644], 'EZ4U': [2653, 2717, 2726], 'carried': [2656], 'out': [2657], '(BD': [2663], '#353072,': [2665], 'flat': [2666], 'bottom),': [2667], 'data': [2669], 'points': [2670], '7500': [2675], 'Jurkats': [2676], 'cultured': [2678, 3085], 'well.': [2688, 3180], 'Plates': [2689, 3235], '48': [2693], 'time,': [2703], 'living': [2707], 'tetrazolium': [2715], 'formazan': [2719], 'derivates': [2720], '(Biozol,': [2721], '#BI-5000).': [2722], '20': [2723, 3318, 3351, 3652], 'reagent': [2727], 'well,': [2732], 'another': [2738], 'h.': [2740, 3494], 'Optical': [2741], '(OD)': [2743], 'ELX800UV': [2748], 'universal': [2749], 'reader': [2751], '(BIO-TEK': [2752], 'Instruments)': [2753], 'wavelength': [2755, 3267, 3274], 'settings': [2756], '465-630': [2758], 'Single': [2760, 4231], 'Imaging—Cells': [2763], 'loaded': [2765, 3221], '22-23': [2767, 3564], 'μm': [2774, 3305, 3327, 3343, 3360, 3815, 4066, 4120, 4360], 'fura-2/AM': [2775], '(Molecular': [2776, 3710], 'Probes)': [2777, 3004, 3315], 'HEPES': [2784], 'fresh': [2788], 'medium,': [2789], 'immediately': [2797], 'allowed': [2801, 3037], 'adhere': [2803], 'poly-l-ornithine-coated': [2805], '(0.1': [2806], 'mg/ml,': [2807, 3013], 'Sigma)': [2808, 3196], 'glass': [2809], 'coverslip': [2810], 'chambers': [2811], 'stage': [2814], 'Olympus': [2817], 'IX': [2818], '70': [2819], 'microscope': [2820], 'equipped': [2821], '20×': [2824], '(UApo/340,': [2825], 'numerical': [2826], 'aperture': [2827], '(N.A.)': [2828], '0.75)': [2829], '40×': [2832], '(Uplan/Apo,': [2833], 'N.A.': [2834], '1.0)': [2835], 'objective.': [2836], 'alternately': [2839], 'illuminated': [2840], '340': [2842], 'nm': [2843, 2846, 2861, 3270, 3439, 3895, 4357, 4388, 4520, 4561], '380': [2845], 'Polychrome': [2849], 'IV': [2850], 'Monochromator': [2851], '(TILL': [2852, 2869, 3706], 'Photonics).': [2853], 'fluorescence': [2855, 3283, 3411], 'emissions': [2856], 'λ': [2858], '>': [2859], '440': [2860], 'captured': [2863], 'charge-coupled': [2866], 'device': [2867], 'camera': [2868], 'Imago),': [2870], 'digitized,': [2871], 'analyzed': [2873, 3698, 3922, 4292, 4315, 4366], 'TILL': [2875, 3704], 'Vision': [2876, 3705], 'software.': [2877], 'Ratio': [2878], 'images': [2879], 'recorded': [2881], 'intervals': [2883], 's.': [2886, 3044, 3287], '[Ca2+]i': [2887, 2893, 3883, 4046, 4096, 4199, 4276], 'estimated': [2889], 'relation': [2892], '=': [2894], 'K*(R': [2895], '-': [2896, 2898], 'Rmin)/(Rmax': [2897], 'R),': [2899], 'where': [2900], 'K*,': [2904], 'Rmin,': [2905], 'Rmax': [2907], 'situ': [2913], 'calibration': [2914], 'fura-2': [2916], '(24Grynkiewicz': [2923], 'Poenie': [2925], 'Tsien': [2927], 'R.Y.': [2928], '1985;': [2932], '260:': [2933], '3440-3450Abstract': [2934], '(80)': [2940], "Ringer's": [2944, 2974, 3113, 3173, 3296, 3321, 3354, 3821, 3865, 4511, 4589], 'contained': [2946, 3509, 3539], 'millimolar):': [2948, 3511, 3541], '155': [2949, 3124, 3542], 'NaCl,': [2950, 3126, 3524, 3543], '4.5': [2951, 3121, 3544], 'KCl,': [2952, 3123, 3545], 'CaCl2,': [2954, 3547], 'MgCl2,': [2956, 3129, 3526, 3549], 'd-glucose,': [2958, 3551], 'Hepes': [2961, 3531, 3554], '(pH': [2962, 3532, 3555], '7.4': [2963, 3556], 'NaOH).': [2965, 3558], 'CaCl2': [2966, 3133], 'replaced': [2968, 2985], 'MgCl2': [2970], '0-Ca2+': [2973, 4510], 'EGTA': [2979, 3301], 'added.': [2980], '145': [2981, 2987], 'NaCl': [2983], 'KCl': [2989], 'TG': [2995, 3306, 3344, 3816, 3829, 4075, 4281, 4423, 4459], '(1': [2996, 3307], 'μm,': [2997], 'stock': [2998, 3010, 3516], 'Me2SO,': [3002, 3311], 'Molecular': [3003, 3164, 3262, 3314], '(10': [3008, 3401], 'hybridoma': [3017], 'OKT3,': [3018], 'ATCC': [3019], 'CRL-8001)': [3020], 'stimulate': [3024, 3384], 'cells.': [3026, 3377, 3409, 4293], 'A': [3027], 'sandwiched': [3028], 'self-made': [3029], 'chamber': [3030], 'measurements,': [3035], 'exchange': [3042], '<1': [3043], 'Imaging': [3046], 'Flexstation—Following': [3049], 'isolation,': [3050], 'kept': [3054], 'streptomycin': [3071], '7': [3080, 4175], 'days.': [3081], '(170': [3104], 'RT)': [3109], '(5': [3115], 'HEPES,': [3117], 'glucose,': [3120], '7.4)/0.5': [3131], 'cells/ml': [3143], '(CD4+)': [3144], '(Jurkat).': [3149], '150': [3150], 'suspension': [3154], 'dye': [3160, 3225, 3445, 3465], '(Ca2+': [3161], 'Assay': [3162], 'Kit,': [3163], 'Devices,': [3165], 'Sunnyvale,': [3166], 'CA;': [3167], 'dissolved': [3168], 'solution/0.5': [3174, 3322], 'CaCl2)': [3176], '(Black': [3184], 'Bottom': [3185], 'Plates,': [3186], 'Corning)': [3187], 'poly-l-ornithine': [3193], '(0.01%': [3194], 'solution,': [3195], 'RT.': [3201], 'settled': [3204], 'bottom': [3207], 'plate': [3210, 3454], '(430': [3213], '45': [3227], 'temperature': [3240], 'dark.': [3246], 'imaging': [3248, 4238, 4499], 'scanning': [3254], 'fluorometer': [3255], 'integrated': [3257], 'fluid': [3258], 'transfer': [3259], 'workstation': [3260], '(Flexstation,': [3261], 'Devices)': [3263], 'excitation': [3266], '485': [3269], 'emission': [3273], '525': [3276], 'Data': [3278, 3695, 4320, 4434], 'relative': [3282], 'units': [3284], 'every': [3285, 3674], '8': [3286], 'stores': [3289, 3848], 'depleted': [3291], '13': [3293], 'concentration)/1': [3304, 3342], 'Calbiochem': [3312], 'adding': [3317, 3350], 'CaCl2/16.5': [3324], 'EGTA/11': [3326], 'TG.': [3328, 3361], 'induced': [3332, 3812], 'subsequent': [3334, 4425], 're-addition': [3335], 'extracellular': [3347, 3827], 'solution/24': [3355], 'CaCl2/1': [3357], 'EGTA/1': [3359], 'calculated': [3365, 3390], 'percentage': [3368, 3392], 'peak': [3371, 3396, 3937, 3976, 4147], 'Me2SO-': [3374, 3406], 'BTP2-treated': [3376, 3408], 'case,': [3379], 'plateau': [3399, 3945, 3982, 4153, 4308, 4614], 'after': [3403, 3662, 3946], 'stimulation)': [3404], 'Basal': [3410], 'subtracted': [3414], 'before': [3415, 3588, 3647], 'calculation.': [3416], 'To': [3417, 3467, 3643, 3770, 3911, 4469], 'determine': [3418, 3468], 'kinetics': [3420], 'preincubation,': [3422], 'various': [3427, 3488, 4431], 'periods': [3428], 'time': [3430, 3442, 3460, 4372, 4400], 'needed': [3443], 'loading': [3446], 'seeding': [3448], 'included': [3456], 'preincubation': [3459, 3958, 4115, 4371], '(BTP2': [3461], 'loading).': [3466], 'dependence': [3471, 4373], 'concentrations': [3489, 3954, 4062, 4261, 4284], 'quadruplicates.': [3499], 'Electrophysiology—The': [3500], 'pipette': [3502], 'whole': [3505, 3569, 3666], 'patch-clamp': [3507, 3595], 'recordings': [3508], '0.05': [3512], '(50': [3514], 'H2O,': [3518], 'Calbiochem)': [3519], '140': [3520], 'Cs': [3521], 'aspartate,': [3522], '1-5': [3525], 'EGTA,': [3528], '7.2': [3533], 'CsOH).': [3535], 'bath': [3537, 3693], 'Patch-clamp': [3559], 'tight-seal': [3568], 'configuration': [3571], 'fire-polished': [3573], 'patch': [3574], 'pipettes': [3575], '(2-5': [3576], 'MΩ': [3577], 'uncompensated': [3578], 'series': [3579], 'resistance).': [3580], 'Pipette': [3581], 'capacitance': [3584], 'electronically': [3586], 'cancelled': [3587], 'voltage': [3590, 3653, 3671], 'ramp': [3591], 'EPC-9': [3594], 'amplifier': [3596], 'controlled': [3597], 'Pulse': [3599], '8.4': [3600], 'software': [3601], '(Heka': [3602], 'Elektronik,': [3603], 'Membrane': [3605], 'currents': [3606, 3623, 3646], 'filtered': [3608], '1.5-2.3': [3610], 'kHz': [3611], 'digitized': [3613], 'sampling': [3616], 'rate': [3617, 3929, 3971, 4142], '5-10': [3619], 'kHz.': [3620], 'Whole': [3621], 'elicited': [3625], '200-ms': [3627], 'voltage-clamp': [3628], 'ramps': [3629, 3654, 3672], '-100': [3631], 'mV': [3632, 3635, 3687], '+100': [3634], 'holding': [3638], 'potential': [3639, 3684, 3785], '0': [3641, 3818], 'mV.': [3642], 'measure': [3644], 'leak': [3645], 'currents,': [3651], 'within': [3657, 4393, 4454], 's': [3661, 3948], 'establishment': [3663], 'configuration,': [3668], 'second.': [3675], 'All': [3676], 'voltages': [3677], 'corrected': [3679], 'liquid': [3682], 'junction': [3683], '-12': [3686], 'between': [3688, 4057, 4109, 4355], 'internal': [3689, 3847], 'solutions': [3690], 'Analysis—Data': [3696], 'programs,': [3702], 'Photonics),': [3707], 'SoftMax': [3708], 'Pro': [3709, 3713], 'Devices),': [3711], 'Igor': [3712], '(Wavemetrics),': [3714], 'Microsoft': [3715], 'Excel': [3716], '(Microsoft),': [3717], 'GraphPad': [3719], 'Prism': [3720], '(GraphPad': [3721], 'Software': [3722], 'Inc.).': [3723], 'Averages': [3724], 'presented': [3726], 'mean': [3728, 4324], '±': [3729, 4325], 'S.D.': [3730], 'S.E.': [3732], 'indicated.': [3734], 'statistical': [3736, 4617], 'analysis': [3737, 4233, 4618], 'paired': [3739], 'unpaired': [3741], 'two-tailed': [3742], 't': [3743], 'test': [3744, 4470], 'Store-operated': [3747], 'major': [3755], 'route': [3758], 'lymphocytes,': [3760, 3792], 'necessary': [3764], 'analyze': [3771], 'possibility': [3773], 'derivative': [3778], '(also': [3780], 'called': [3781], 'YM-58483),': [3782], 'inhibit': [3787], 'presence': [3797, 4558], 'absence': [3799, 3825], 'compared.': [3803], 'Depletion': [3804], 'Ca2+,': [3828], 'causes': [3830], 'small,': [3833, 4081], 'transient': [3834, 4555], 'rise': [3836], 'PBLs': [3838, 3999, 4056, 4078, 4475], 'resulting': [3839, 3877], 'unopposed': [3842], 'leakage': [3843], 'extrusion': [3852], 'across': [3853, 3873], 'membrane.': [3856], 'Exchanging': [3857], 'external': [3859], 'allows': [3867], 'long': [3879], 'lasting': [3880], 'elevations': [3881], 'illustrated': [3885], 'Fig.': [3887, 4247, 4409, 4438, 4495, 4648], '1A.': [3888], 'Preincubation': [3889], 'leads': [3900], 'marked': [3903, 4135], 'decrease': [3904, 4541], '(Fig.': [3909, 3972, 3977, 3983, 4091, 4125, 4143, 4148, 4154, 4407, 4532, 4593, 4619], '1A).': [3910], 'quantify': [3912], 'signals,': [3918], 'parameters': [3920], 'single': [3924, 4651], 'cells:': [3925], 'initial': [3927, 3968, 4139, 4299, 4604], 'fitted': [3931, 3938], 'regression,': [3934, 3941], 'polynomial': [3940], '300': [3947, 4356], '(averaging': [3949], 'points).': [3951], 'Analyzing': [3952, 4094], 'varying': [3953], 'resulted': [3961], 'dose-dependent': [3964, 4295], '1B),': [3973], '1C),': [3978], '1D).': [3984], 'sigmoidal': [3990, 4166], 'regressions': [3991, 4167], '6,': [3993], '14,': [3994], '12': [3996], 'nm,': [3997, 4176], 'respectively.': [3998, 4177], 'mix': [4002], '(about': [4006], '50%': [4007], '25%': [4010], 'T-cells).': [4012], 'Because': [4013, 4070, 4650], 'showed': [4016], 'homogenous': [4018], '(data': [4023, 4067, 4361], 'shown),': [4025], 'concluded': [4028], 'reduced': [4039], 'No': [4042], 'difference': [4043], 'resting': [4045, 4095, 4198, 4275], 'TG-dependent': [4050, 4098, 4202], 'release': [4052, 4072, 4100, 4204, 4218, 4279, 4517, 4530, 4554], 'observed': [4054, 4108, 4592], 'conditions': [4059, 4111, 4567], 'shown).': [4069, 4363], 'same': [4083], 'measurements': [4084, 4415, 4623], 'repeated': [4086], '2A,': [4092], 'inset).': [4093], 'levels,': [4097], 'peaks,': [4101], 'extrusion,': [4104], 'no': [4105, 4272], 'differences': [4106], '(no': [4112], 'BTP2)': [4113], '2A).': [4126], 'Similar': [4127], 'there': [4131], 'was,': [4132], '2B),': [4144], '2C),': [4149], '2D)': [4155], '5,': [4172], '10,': [4173], 'conclude': [4179], 'CD4+,': [4187], 'CD8+,': [4188], 'low': [4194, 4228], 'nanomolar': [4195, 4229], 'range,': [4196], 'whereas': [4197], 'affected.Fig.': [4211], '2Store-operated': [4212], 'range.': [4230], 'set-up.': [4239], 'A,': [4240], 'typical': [4242], '1A': [4248], 'inset.': [4253], 'symbols': [4255], 'refer': [4256], 'average': [4259], 'depicted': [4262, 4646], 'lower': [4265], 'part': [4266], 'figure.': [4269], 'effect': [4273, 4368], 'μm.': [4287], 'Points': 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