Publication Information

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'https://openalex.org/W1973001216'], 'abstract_inverted_index': {'The': [0, 52, 91, 149, 224, 276, 315, 373, 838, 1449, 1886, 2190, 2427, 2634, 2800, 2872, 2886, 2967, 3010, 3091, 3121, 3228, 3296, 3365, 3461, 3507, 3819, 3844], 'immunosuppressant': [1, 225, 839], 'rapamycin,': [2, 226], 'in': [3, 30, 57, 67, 110, 123, 176, 188, 227, 254, 281, 291, 334, 347, 400, 412, 548, 1042, 1134, 1172, 1221, 1336, 1434, 1891, 2020, 2045, 2230, 2315, 2356, 2432, 2488, 2526, 2542, 2810, 2877, 2918, 2974, 2982, 3004, 3021, 3063, 3096, 3105, 3114, 3187, 3292, 3414, 3454, 3537, 3551, 3576, 3590, 3695, 3743, 3751, 3831, 3873, 3903, 3939, 3978, 4002, 4027, 4038, 4048, 4066, 4090, 4115, 4126, 4174, 4220, 4235, 4466], 'complex': [4, 228, 1987, 4260, 4416, 4468], 'with': [5, 205, 229, 429, 1416, 1972, 2157, 2310, 2555, 2719, 2925, 3015, 3126, 3137, 3235, 3240, 3248, 3304, 3370, 3374, 3525, 3616, 3756, 3905, 4450, 4472], 'its': [6, 49, 230, 273, 2158, 2466], 'cellular': [7, 12, 231, 236, 1008, 1230, 1341], 'receptor': [8, 232], 'FKBP12,': [9, 233, 576, 646], 'targets': [10, 234], 'the': [11, 37, 73, 83, 101, 128, 190, 198, 203, 235, 261, 297, 307, 325, 352, 414, 422, 427, 466, 478, 550, 562, 1004, 1007, 1173, 1222, 1229, 1318, 1428, 1438, 1444, 1458, 1463, 1676, 1766, 1881, 1903, 1962, 1985, 2046, 2148, 2196, 2216, 2311, 2318, 2448, 2453, 2460, 2471, 2479, 2485, 2497, 2583, 2596, 2613, 2616, 2628, 2708, 2723, 2734, 2740, 2754, 2834, 2856, 2910, 2957, 3049, 3082, 3183, 3293, 3385, 3431, 3445, 3457, 3471, 3475, 3494, 3504, 3526, 3532, 3546, 3579, 3584, 3601, 3604, 3612, 3624, 3660, 3685, 3731, 3769, 3790, 3802, 3806, 3814, 3823, 3852, 3871, 3914, 4022, 4042, 4110, 4130, 4138, 4161, 4206, 4209, 4217, 4229, 4256, 4292, 4410, 4451, 4460, 4467, 4504], 'protein': [13, 116, 237, 340, 469, 611, 681, 745, 1009, 1046, 1422, 1451, 1682, 1877, 2944, 3549, 3630, 3760, 3783, 3798, 3853, 3883, 3910, 4141], 'FKBP12-rapamycin-associated': [14, 238, 580, 650], 'protein/mammalian': [15, 239], 'target': [16, 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1884, 1961, 2198, 2447, 2462, 2512, 2523, 2567, 2964, 3007, 3099, 3109, 3118, 3132, 3175, 3306, 3329, 3362, 3389, 3420, 3437, 3680, 3992, 4010, 4080, 4098, 4157, 4181, 4243], 'signaling': [50, 274, 1577, 3592], 'function.': [51, 275], 'FKBP12-rapamycin': [53, 277, 1887, 2906, 3562, 4512], 'binding': [54, 278, 744, 1888, 2323, 2510, 2907, 2922, 2978, 3337, 4179, 4507], '(FRB)': [55, 279, 1889], 'domain': [56, 106, 109, 192, 280, 330, 333, 416, 1415, 1890, 1905, 1958, 2150, 2431, 2442, 2487, 3434, 3447, 3463, 3589, 4173], 'FRAP': [58, 68, 157, 186, 217, 282, 292, 381, 410, 441, 1236, 1315, 1450, 1668, 1694, 1749, 1813, 1892, 1965, 2013, 2201, 2228, 2351, 2392, 2457, 2501, 2667, 2717, 2758, 2770, 2779, 3390, 3403, 3580, 4175, 4202, 4305, 4526], 'also': [60, 152, 284, 376, 1743, 2436], 'speculated': [61, 285], 'to': [62, 130, 219, 286, 354, 443, 465, 556, 1317, 1419, 1470, 1578, 1675, 1688, 1693, 1746, 1801, 1874, 1902, 2065, 2226, 2307, 2394, 2398, 2438, 2591, 2815, 2892, 2956, 3053, 3071, 3075, 3081, 3084, 3159, 3169, 3456, 3501, 3560, 3610, 3789, 3870, 3980, 3985, 4068, 4073, 4215, 4225, 4240, 4290, 4311, 4401, 4511, 4530], 'play': [63, 287], 'an': [64, 206, 288, 430, 742, 1763, 1897, 2056, 2513, 3779, 4183, 4190], 'important': [65, 289], 'role': [66, 99, 290, 323, 2185], 'function': [69, 218, 293, 442, 2192, 2458, 2473, 2518, 3593, 3613, 4502], 'signaling.': [71, 295, 4203], 'However,': [72, 296, 1662, 3513, 4301], 'biochemical': [74, 298, 2191], 'physiological': [76, 300, 1882, 2472], 'functions': [77, 301, 1445, 4176, 4404], 'FRB,': [79, 303, 582, 652, 2505, 4221], 'as': [80, 82, 304, 306, 1169, 1171, 1228, 1896, 2186, 2580, 3348, 3594, 3766, 3793, 3972, 4021, 4060, 4109, 4143, 4177, 4212, 4214, 4434], 'well': [81, 305, 1170, 2153, 3691, 4213], 'mechanism': [84, 308, 2197, 2461], 'rapamycin': [86, 310, 588, 602, 658, 672, 840, 2018, 2067, 2213, 2224, 2364, 2463, 2915, 3597, 3688, 4244, 4407, 4474], 'inhibition,': [87, 311], 'have': [88, 312, 553, 1685, 1814, 1976, 2469, 3394], 'been': [89, 313, 554, 1226, 1425, 1468, 1686, 1744, 1815, 1894, 2208, 2640, 3678, 4238], 'unclear.': [90, 314, 2204], 'present': [92, 316], 'study': [93, 317, 4291], 'focuses': [94, 318], 'on': [95, 319, 2948, 2988, 3327, 3813], 'investigation': [96, 320, 1798], "FRB's": [98, 322, 4500], 'functional': [102, 326, 2188, 2480, 2504], 'relationship': [103, 327, 2481, 4219], 'between': [104, 328, 1964, 2482, 4462], 'FRB': [105, 115, 170, 191, 329, 339, 394, 415, 2149, 2194, 2430, 2483, 2520, 2579, 2586, 2605, 2857, 2888, 2911, 2943, 3446, 3478, 3572, 3588, 3615, 3629, 3666, 3759, 3791, 3824, 3837, 3847, 3892, 3934, 4140, 4163, 4194, 4210, 4231, 4236, 4297, 4443, 4463], 'FRAP.': [111, 335, 1885, 2489], 'Microinjection': [112, 336], 'purified': [114, 338, 2861, 2887, 3631], 'into': [117, 341, 2595, 2707, 2739, 3667], 'human': [118, 342, 2530, 2535], 'osteosarcoma': [119, 343, 2531, 3673], 'MG63': [120, 344, 2532, 2993, 3674, 3967, 4055], 'cells': [121, 345, 1139, 2022, 2533, 2539, 2994, 3012, 3040, 3093, 3122, 3146, 3184, 3669, 3754, 3839, 3865, 3878, 3904, 3938, 3968, 3998, 4007, 4026, 4034, 4056, 4086, 4095, 4114, 4122], 'results': [122, 346, 1041], 'drastic': [125, 349], 'blockage': [126, 350], 'S': [131, 355, 3739, 3764, 3940, 4011, 4028, 4039, 4099, 4116, 4127], 'progression;': [134, 358], 'such': [135, 359, 462, 3438], 'dominant': [137, 361, 4145], 'negative': [138, 362, 4146], 'effect': [139, 363, 3661, 3689, 3775, 3812, 3825, 3845, 4164, 4211, 4295], 'reversed': [141, 365], 'by': [142, 366, 453, 1812, 2062, 2360, 2608, 2751, 2768, 2784, 2854, 2938, 3103, 3270, 3311, 3351, 3380, 3596, 3670, 3727, 3768, 3828, 3913, 4014, 4102], 'point': [144, 368, 846], 'mutation': [145, 151, 369, 375, 2735, 4494], '(Trp2027': [146, 370], '→': [147, 371, 2618, 2622, 2636, 2671, 2675, 2721, 3950, 3955, 3960, 4523], 'Phe).': [148, 372], 'same': [150, 374, 3803, 4043, 4131], 'abolishes': [153, 377], 'without': [158, 382, 3480, 4035, 4123], 'affecting': [159, 383], 'ATP': [160, 384, 3336], 'binding,': [161, 385], 'truncation': [163, 387, 2760], 'studies': [164, 388], 'suggest': [165, 389, 1747], 'that': [166, 390, 1748, 1757, 1975, 2212, 2440, 2496, 3493, 3531, 4137, 4148, 4171, 4303, 4446], 'upstream': [167, 207, 391, 431, 847, 1670, 2514, 3452, 3482, 3574, 4184], 'sequences': [168, 392, 2771, 3453, 3483, 3575], 'including': [169, 393, 1343], 'are': [171, 395, 1334, 2202, 4019, 4037, 4107, 4125], 'required': [172, 194, 396, 418, 1575, 1800, 2225, 2446, 3436], 'vitro.': [177, 401], 'Given': [178, 402, 3583], 'these': [179, 403, 1447, 1803, 2015, 3538, 3552, 3681], 'data,': [180, 404, 3607], 'we': [181, 405, 2468, 2491, 3393, 3542, 3608, 3658, 4222, 4437], 'propose': [182, 406], 'model': [184, 408], 'function,': [187, 411, 4152], 'which': [189, 413, 471, 1326, 1466, 1876, 2506, 4153], 'activation': [196, 420], 'domain,': [200, 424, 1462], 'possibly': [201, 425], 'through': [202, 426, 2627], 'interaction': [204, 428, 1963], 'activator.': [208, 432], 'In': [209, 433, 2390], 'addition,': [210, 434, 2391], 'our': [211, 435], 'observations': [212, 436, 3567, 4135], 'provide': [213, 437, 2508], 'direct': [214, 438, 3619], 'evidence': [215, 439, 1741, 1870, 2494], 'linking': [216, 440], 'progression.': [223, 447, 4159], 'Mammalian': [448], 'proliferation': [450], 'regulated': [452], 'extracellular': [454, 1690], 'mitogens': [455], 'via': [456, 2711, 2745], 'multiple': [457], 'signal': [458, 4310], 'transduction': [459], 'pathways.': [460], 'One': [461], 'pathway': [463, 843, 1224, 1756, 1768], 'leads': [464], 'up-regulation': [467], 'synthesis,': [470], '(1Brown': [481], 'E.J.': [482, 872, 1238, 1476, 1582, 1911, 1993, 2024, 2233, 2329, 2648, 2685, 3640, 3704, 4266, 4317], 'Schreiber': [483, 881, 1023, 1152, 1249, 1357, 1485, 1591, 1912, 1994, 2033, 2131, 2169, 2242, 2330, 2649, 2694, 3641, 3709, 4267, 4326, 4421, 4481], 'S.L.': [484, 882, 1024, 1153, 1250, 1358, 1486, 1592, 1913, 1995, 2034, 2132, 2170, 2243, 2331, 2650, 2695, 3642, 3710, 4268, 4327, 4422, 4482], 'Cell.': [485, 824, 1193, 1271, 1370, 1384, 2090, 2114, 2133], '1996;': [486, 2174, 2419, 4426, 4486], '86:': [487], '517-520Abstract': [488], 'Full': [489, 491, 828, 830, 935, 937, 1275, 1305, 1307, 1374, 1388, 1403, 1405, 1537, 1539, 1652, 1654, 1787, 1789, 2137, 3717, 4387, 4389], 'Text': [490, 492, 829, 831, 936, 938, 1276, 1306, 1308, 1375, 1389, 1404, 1406, 1538, 1540, 1653, 1655, 1788, 1790, 2138, 3718, 4388, 4390], 'PDF': [493, 832, 939, 1277, 1309, 1376, 1390, 1407, 1541, 1656, 1791, 2139, 3719, 4391], 'PubMed': [494, 720, 736, 787, 813, 833, 866, 887, 913, 940, 970, 996, 1035, 1070, 1093, 1110, 1164, 1198, 1213, 1255, 1278, 1310, 1363, 1377, 1391, 1408, 1491, 1517, 1542, 1567, 1597, 1623, 1657, 1710, 1735, 1792, 1838, 1863, 1924, 1945, 2006, 2039, 2095, 2118, 2140, 2177, 2248, 2274, 2300, 2342, 2385, 2422, 2661, 2700, 3653, 3720, 4279, 4332, 4358, 4392, 4429, 4489], 'Scopus': [495, 721, 737, 788, 814, 834, 867, 888, 914, 941, 971, 997, 1036, 1071, 1094, 1111, 1165, 1199, 1214, 1256, 1279, 1311, 1364, 1378, 1392, 1409, 1492, 1518, 1543, 1568, 1598, 1624, 1658, 1711, 1736, 1793, 1839, 1864, 1925, 1946, 2007, 2040, 2096, 2119, 2141, 2178, 2249, 2275, 2301, 2343, 2386, 2423, 2662, 2701, 3654, 4280, 4333, 4359, 4393, 4430, 4490], '(341)': [496], 'Google': [497, 723, 739, 790, 816, 836, 869, 890, 916, 943, 973, 999, 1038, 1073, 1096, 1113, 1130, 1167, 1201, 1216, 1258, 1281, 1313, 1366, 1380, 1394, 1411, 1494, 1520, 1545, 1570, 1600, 1626, 1660, 1713, 1738, 1795, 1841, 1866, 1927, 1948, 2009, 2042, 2098, 2121, 2143, 2180, 2251, 2277, 2303, 2345, 2388, 2425, 2664, 2703, 3656, 3721, 4282, 4335, 4361, 4395, 4432, 4492], 'Scholar,': [498, 521, 724, 767, 791, 817, 870, 917, 944, 974, 1074, 1097, 1114, 1202, 1367, 1381, 1395, 1495, 1521, 1546, 1601, 1627, 1842, 1928, 2099, 2122, 2252, 2278, 4336, 4362], '2Jefferies': [499], 'H.B.J.': [500], 'Thomas': [501, 710, 1081], 'G.': [502, 711, 903, 1022, 1082, 1151, 1296, 2411], 'Hershey': [503, 524, 749], 'J.W.B.': [504, 525, 750], 'Mathews': [505, 526, 751], 'M.B.': [506, 527, 752], 'Sonenberg': [507, 528, 753, 781, 904, 1701], 'N.': [508, 523, 529, 748, 754, 782, 905, 1049, 1116, 1702, 1704], 'Translational': [509, 530, 755], 'Control.': [510, 531, 756], 'Cold': [511, 516, 532, 537, 757, 762], 'Spring': [512, 517, 533, 538, 758, 763], 'Harbor': [513, 534, 759], 'Laboratory': [514, 535, 760], 'Press,': [515, 536, 761], 'Harbor,': [518, 539, 764], 'NY1996:': [519, 540, 765], '389-409Google': [520], '3Sonenberg': [522], '245-269Google': [541, 766], 'Scholar).': [542, 837, 1217, 1314, 1412, 1661, 1796, 2010, 2043, 2144, 2346, 2389, 2426, 2665, 3722, 4283], 'At': [543, 3037, 3178], 'least': [544, 2902, 3038, 3555], 'two': [545, 3238, 4439], 'proteins': [546, 2807, 2823, 2836, 2853, 2858, 2889, 3017, 3410], 'involved': [547, 1335], 'regulating': [549], 'translational': [551], 'machinery': [552], 'found': [555, 1426, 2061, 2397, 4528], 'lie': [557], 'downstream': [558, 1677, 4188], 'this': [560, 842, 1001, 1332, 1435, 1957, 1970, 2441, 2516, 3696, 4172, 4197, 4249, 4397, 4496], 'pathway:': [561], 'p70': [563], 'S6': [564, 574, 644], '(p70s6k)': [566], '1The': [567], 'abbreviations': [568, 638], 'used': [569, 639, 2566, 3168], 'are:': [570, 640], 'p70s6k,': [571, 641], '70': [572, 642], 'kDa': [573, 643], 'kinase;': [575, 608, 645, 678], 'FK506-binding': [577, 647], 'protein;': [578, 581, 648, 651], 'FRAP,': [579, 649, 2050, 2220, 3340], 'FKBP12-rapamycin-binding': [583, 653], '(domain);': [584, 654], 'rap,': [585, 655], 'rapamycin;': [586, 597, 656, 667], 'RAFT1,': [587, 657], '1;': [592, 612, 662, 682], 'mTOR,': [593, 663], 'TOR1,': [598, 668], 'TOR2,': [599, 669, 2053], '2;': [605, 675], 'PIK,': [606, 676], '4E-BP1,': [609, 679], 'eIF4E-binding': [610, 680], 'GST,': [613, 683], 'glutathione': [614, 684], 'S-transferase;': [615, 685], 'PCR,': [616, 686], 'polymerase': [617, 687], 'chain': [618, 688], 'reaction;': [619, 689], 'DTT,': [620, 690, 3216], 'dithiothreitol;': [621, 691], 'PBS,': [622, 692], 'phosphate-buffered': [623, 693, 2878], 'saline;': [624, 694], 'PAGE,': [625, 695], 'polyacrylamide': [626, 696], 'gel': [627, 697, 3226], 'electrophoresis;': [628, 698], 'DMEM,': [629, 699], "Dulbecco's": [630, 700], 'modified': [631, 701], "Eagle's": [632, 702], 'medium;': [633, 703], 'FBS,': [634, 704], 'fetal': [635, 705, 2546], 'bovine': [636, 706, 2547], 'serum.1The': [637], 'serum.': [707], '(4Ferrari': [708], 'S.': [709, 795, 808, 965, 1030, 1065, 1088, 1159, 1562, 1730, 1858, 1919, 1940, 2001, 2295, 2337, 2380, 2656, 3648, 4274], 'Crit.': [712], 'Rev.': [713], 'Biochem.': [714], 'Mol.': [715, 1192, 2089, 2112], 'Biol.': [716, 732, 930, 1194, 1300, 1399, 1532, 1647, 1782, 2091, 2113, 3712, 4382], '1994;': [717, 784, 810, 910, 1067, 1090, 1252, 1272, 1942, 2115], '29:': [718], '385-413Crossref': [719], '(133)': [722], '5Chou': [725], 'M.M.': [726, 1288, 2074], 'Blenis': [727, 858], 'J.': [728, 853, 859, 878, 929, 1103, 1105, 1125, 1204, 1299, 1482, 1531, 1588, 1645, 1646, 1780, 1781, 1907, 1989, 2030, 2105, 2128, 2166, 2168, 2172, 2239, 2325, 2407, 2418, 2644, 2691, 3636, 3711, 4262, 4323, 4380, 4381, 4418, 4420, 4424, 4478, 4480, 4484], 'Curr.': [729, 1398], 'Opin.': [730], 'Cell': [731], '1995;': [733, 884, 932, 967, 1107, 1127, 1302, 1360, 1371, 1385, 1400, 1488, 1594, 1921, 2003, 2036, 2134, 2245, 2339, 2658, 2697, 3650, 4276, 4329], '7:': [734], '806-814Crossref': [735], '(245)': [738, 2142], 'Scholar)': [740, 891, 1039, 1131, 1571, 1949, 2304, 2704, 4433], 'eIF4E': [743], '(4E-BP1)': [746], '(3Sonenberg': [747], '6Pause': [768], 'A.': [769, 809, 901, 966, 980, 1031, 1066, 1089, 1160, 1501, 1563, 1607, 1731, 1822, 1859, 1920, 1941, 2002, 2258, 2296, 2338, 2381, 2657, 3649, 3706, 4275, 4342], 'Belsham': [770, 902], 'G.J.': [771, 976, 1290, 1497, 1523, 1603, 1717, 1818, 2254, 2282, 2405, 4338], 'Gingras': [772], 'A.C.': [773, 1057, 1122, 1696], 'Donze': [774], 'O.': [775], 'Lin': [776, 955], 'T.A.': [777, 895, 899, 956], 'Lawrence': [778, 800, 906, 926, 957, 987, 1508, 1528, 1614, 1722, 1829, 2265, 2287, 2412, 4349], 'Jr.,': [779, 801, 907, 927, 958, 988, 1509, 1529, 1615, 1723, 1830, 2266, 2288, 2413, 4350], 'J.C.': [780, 802, 908, 928, 959, 989, 1510, 1530, 1616, 1724, 1831, 2267, 2289, 2414, 4351], 'Nature.': [783, 862, 883, 1106, 1251, 1487, 1593, 2035, 2244, 2696, 4328], '371:': [785], '762-767Crossref': [786], '(1053)': [789], '7Hu': [792], 'C.': [793, 1078, 1778, 2409], 'Pang': [794], 'Kong': [796, 896, 920, 953], 'X.': [797, 897, 921, 954], 'Velleca': [798], 'M.': [799, 1266, 1641, 2107, 4376], 'Proc.': [803, 960, 1025, 1060, 1083, 1154, 1557, 1725, 1853, 1914, 1935, 1996, 2290, 2332, 2375, 2651, 3643, 4269], 'Natl.': [804, 961, 1026, 1061, 1084, 1155, 1558, 1726, 1854, 1915, 1936, 1997, 2291, 2333, 2376, 2652, 3644, 4270], 'Acad.': [805, 962, 1027, 1062, 1085, 1156, 1559, 1727, 1855, 1916, 1937, 1998, 2292, 2334, 2377, 2653, 3645, 4271], 'Sci.': [806, 963, 1028, 1063, 1086, 1157, 1560, 1728, 1856, 1917, 1938, 1999, 2293, 2335, 2378, 2654, 3646, 4272], 'U.': [807, 964, 1029, 1064, 1087, 1158, 1561, 1729, 1857, 1918, 1939, 2000, 2294, 2336, 2379, 2655, 3647, 4273], '91:': [811, 1068, 1091, 1943], '3730-3734Crossref': [812], '(125)': [815], '8Sachs': [818], 'A.B.': [819], 'Sarnow': [820], 'P.': [821, 1120, 1268, 1525, 2103], 'Hentze': [822], 'M.W.': [823, 1240, 3700], '1997;': [825, 993, 1514, 1534, 1620, 1649, 1835, 2271, 4355, 4384], '89:': [826], '831-838Abstract': [827], '(587)': [835], 'inhibits': [841, 4149, 4201], 'at': [844, 1427, 1969, 2055, 2552, 2797, 2812, 2826, 2901, 2933, 2961, 2999, 3018, 3060, 3301, 3359, 3554, 3725, 3801, 3842, 3854, 4248, 4299, 4459, 4495], 'p70s6k': [849, 1809, 2353, 4312], '(9Kuo': [850], 'C.J.': [851, 1286], 'Chung': [852], 'Fiorentino': [854], 'D.F.': [855], 'Flanagan': [856], 'W.M.': [857], 'Crabtree': [860, 1021, 1150, 2129], 'G.R.': [861, 2130], '1992;': [863], '358:': [864], '70-73Crossref': [865], '(564)': [868], '10Brown': [871], 'Beal': [873, 1477, 1583, 2025, 2234, 2686, 4318], 'P.A.': [874, 1478, 1584, 2026, 2235, 2687, 4319], 'Keith': [875, 1245, 1479, 1585, 2027, 2236, 2688, 4320], 'C.T.': [876, 1246, 1356, 1480, 1586, 2028, 2237, 2689, 4321], 'Chen': [877, 1481, 1587, 2029, 2127, 2167, 2238, 2690, 4322, 4419, 4479], 'Shin': [879, 1241, 1483, 1589, 2031, 2240, 2692, 4324], 'T.B.': [880, 1242, 1484, 1590, 2032, 2241, 2693, 4325], '377:': [885, 1108, 1489, 1595, 2037, 2246, 2698, 4330], '441-446Crossref': [886, 1490, 1596, 2038, 2247, 2699, 4331], '(617)': [889, 1493, 1599, 2041, 2250, 2702, 4334], '4E-BP1': [893, 1580, 1811, 2355, 3517, 4314], '(11Lin': [894], 'Haystead': [898, 1526], 'Pause': [900], 'Science.': [909, 992, 1209, 1359, 1513, 1619, 1834, 2173, 2270, 4354, 4425, 4485], '266:': [911], '653-656Crossref': [912], '(599)': [915], '12Lin': [918], 'T.-A.': [919], 'Saltiel': [922], 'A.R.': [923], 'Blackshear': [924], 'P.J.': [925, 986, 1507, 1613, 1828, 2264, 4348], 'Chem.': [931, 1301, 1533, 1648, 1783, 3713, 4383], '270:': [933, 1303, 1361], '18531-18538Abstract': [934], '(234)': [942], '13Graves': [945], 'L.M.': [946], 'Bornfeldt': [947], 'K.E.': [948], 'Argast': [949], 'G.M.': [950, 2080], 'Krebs': [951], 'E.G.': [952], '92:': [968, 1922, 2004, 2340, 2659, 3651, 4277], '7222-7226Crossref': [969], '(208)': [972], '14Brunn': [975, 1496, 1602, 2253, 4337], 'Hudson': [977, 1498, 1604, 1819, 2255, 4339], 'C.C.': [978, 1499, 1605, 1820, 2256, 4340], 'Sekulic': [979, 1500, 1606, 1821, 2257, 4341], 'Williams': [981, 1291, 1502, 1608, 1823, 2259, 2406, 3701, 4343], 'J.M.': [982, 1292, 1503, 1609, 1824, 2260, 4344], 'Hosoi': [983, 1504, 1610, 1825, 2261, 4345], 'H.': [984, 1264, 1505, 1611, 1826, 1932, 2262, 4346], 'Houghton': [985, 1506, 1612, 1827, 2263, 4347], 'Abraham': [990, 1297, 1511, 1617, 1832, 2268, 2415, 4352], 'R.T.': [991, 1298, 1512, 1618, 1833, 2269, 2416, 3702, 4353], '277:': [994, 1515, 1621, 1836, 2272, 4356], '99-101Crossref': [995, 1516, 1622, 1837, 2273, 4357], '(804)': [998, 1519, 1625, 1840, 2276, 4360], 'Scholar);': [1000, 1867, 4493], 'inhibition': [1002, 2464], 'requires': [1003, 2502], 'presence': [1005], '(15Bierer': [1011, 1140], 'B.E.': [1012, 1141], 'Mattila': [1013, 1142], 'P.S.': [1014, 1143], 'Standaert': [1015, 1144], 'R.F.': [1016, 1145], 'Herzenberg': [1017, 1146], 'L.A.': [1018, 1147], 'Burakoff': [1019, 1148], 'S.J.': [1020, 1149], '1990;': [1032, 1161], '87:': [1033, 1162], '9231-9235Crossref': [1034, 1163], '(627)': [1037, 1166], 'selective': [1043], 'reduction': [1044], 'synthesis': [1047], '(16Terada': [1048], 'Patel': [1050], 'H.R.': [1051], 'Takase': [1052, 1117], 'K.': [1053, 1055, 1118, 1244, 1629, 1631, 1637, 1770, 1772, 4364, 4366, 4372], 'Kohno': [1054], 'Nairn': [1056, 1121], 'Gelfand': [1058, 1123], 'E.W.': [1059, 1124], '11477-11481Crossref': [1069], '(319)': [1072], '17Jefferies': [1075], 'H.B.': [1076], 'Reinhard': [1077], 'Kozma': [1079], 'S.C.': [1080], '4441-4445Crossref': [1092], '(553)': [1095], '18Nielsen': [1098], 'F.C.': [1099], 'Ostergaard': [1100], 'L.': [1101, 1179, 2082], 'Nielsen': [1102], 'Christiansen': [1104], '358-362Crossref': [1109], '(158)': [1112], '19Terada': [1115], 'Papst': [1119], 'Immunol.': [1126], '155:': [1128], '3418-3426PubMed': [1129], 'G1arrest': [1133], 'variety': [1136], 'Scholar),': [1168, 1259, 1282, 1739, 2181, 3657, 4396], 'yeastSaccharomyces': [1174], 'cerevisiae': [1175], '(20Koltin': [1176], 'Y.': [1177, 2078], 'Faucette': [1178, 2081], 'Bergsma': [1180, 2075], 'D.J.': [1181, 2076], 'Levy': [1182], 'M.A.': [1183, 1700], 'Cafferkey': [1184], 'R.': [1185, 2070, 2109], 'Koser': [1186], 'P.L.': [1187], 'Johnson': [1188, 2085], 'R.K.': [1189, 1550, 1846, 2086, 2368], 'Livi': [1190, 2087], 'G.P.': [1191, 2088], '1991;': [1195, 1210], '11:': [1196], '1718-1723Crossref': [1197], '(191)': [1200], '21Heitman': [1203], 'Movva': [1205], 'N.R.': [1206], 'Hall': [1207, 2110, 3707], 'M.N.': [1208, 2111], '253:': [1211], '905-909Crossref': [1212], '(1528)': [1215], 'A': [1218, 1413, 1952], 'major': [1219], 'player': [1220], 'rapamycin-sensitive': [1223], 'has': [1225, 1424, 1467, 1742, 1893, 2207, 2639, 3677, 4237], 'identified': [1227, 1895], 'rapamycin-FKBP12': [1233, 2395], 'complex,': [1234], 'designated': [1235], '(22Brown': [1237], 'Albers': [1239], 'Ichikawa': [1243], 'Lane': [1247], 'W.S.': [1248], '369:': [1253], '756-758Crossref': [1254], '(1646)': [1257], 'RAFT1': [1260], '(23Sabatini': [1261], 'D.M.': [1262, 1556, 1852, 2374], 'Erdjument-Bromage': [1263], 'Lui': [1265], 'Tempst': [1267], 'Snyder': [1269, 1553, 1849, 2371], 'S.H.': [1270, 1554, 1850, 2372], '78:': [1273], '35-43Abstract': [1274], '(1211)': [1280], 'or': [1283, 3805, 4186], 'mTOR': [1284], '(24Sabers': [1285], 'Martin': [1287], 'Brunn': [1289, 1716, 2281], 'Dumont': [1293], 'F.J.': [1294], 'Wiederrecht': [1295, 2410], '815-822Abstract': [1304], '(699)': [1312], 'belongs': [1316], 'family': [1320, 1333], '(PIK)-related': [1324], 'kinases': [1325, 1423, 2435], 'includeAtaxia': [1327], 'telangiectasia': [1328], 'mutated.': [1329], 'Members': [1330], 'range': [1338], 'functions,': [1342], 'progression,': [1346], 'checkpoints,': [1349], 'DNA': [1350, 1353, 3174, 3772], 'repair,': [1351], 'recombination': [1354], '(25Keith': [1355], '50-51Crossref': [1362], '(443)': [1365], '26Hunter': [1368], 'T.': [1369, 1635, 4370], '83:': [1372], '1-4Abstract': [1373], '(261)': [1379], '27Zakian': [1382], 'V.A.': [1383], '82:': [1386, 2135], '685-687Abstract': [1387], '(277)': [1393], '28Jackson': [1396], 'S.P.': [1397], '5:': [1401, 2116], '1210-1212Abstract': [1402], '(57)': [1410], 'sequence': [1417, 2728], 'homology': [1418], 'lipid': [1420], 'C': [1429, 2161], 'termini': [1430, 2162], 'all': [1432, 4246], 'members': [1433], 'crucial': [1442, 1960, 4242], 'proteins.': [1448, 2576], '289-kDa': [1454], 'single': [1455], 'polypeptide': [1456], 'containing': [1457, 2544, 2669, 2882, 3023, 3055, 3243, 3279, 3473, 4251], 'characteristic': [1459], 'C-terminal': [1460, 3458], 'shown': [1469, 3750, 4239], 'be': [1471, 2308, 3499, 4241], 'toward': [1472, 2352], 'serine/threonine': [1473], 'residues': [1474, 1974], '(10Brown': [1475, 1581, 2023, 2232, 2684, 4316], '29Brunn': [1522], 'Fadden': [1524], 'T.A.J.': [1527], '272:': [1535, 1650, 4385], '32547-32550Abstract': [1536], '(146)': [1544], '30Burnett': [1547, 1843], 'P.E.': [1548, 1844, 2366], 'Barrow': [1549, 1845, 2367], 'Cohen': [1551, 1847, 2369], 'N.A.': [1552, 1848, 2370], 'Sabatini': [1555, 1851, 2373], '1998;': [1564, 1707, 1732, 1784, 1860, 2297, 2382], '95:': [1565, 1733, 1861, 2298, 2383], '1432-1437Crossref': [1566, 1862, 2384], '(929)': [1569, 1865, 2387], 'it': [1573, 1663, 2206], 'p70s6kand': [1579], '31Hara': [1628, 4363], 'Yonezawa': [1630, 1771, 4365], 'Kozlowski': [1632, 1775, 4367], 'M.T.': [1633, 1776, 4368], 'Sugimoto': [1634, 4369], 'Andrabi': [1636, 4371], 'Weng': [1638, 1773, 4373], 'Q.P.': [1639, 4374], 'Kasuga': [1640, 4375], 'Nishimoto': [1642, 4377], 'I.': [1643, 4378], 'Avruch': [1644, 1779, 4379], '26457-26463Abstract': [1651, 4386], '(406)': [1659, 4394], 'currently': [1665, 2203, 3511], 'unclear': [1666], 'how': [1667], 'receives': [1669], 'signals': [1671], 'transduces': [1673], 'them': [1674], 'components.': [1678], 'Phosphatidylinositol': [1679], '3-kinase': [1680], 'B/Akt': [1684], 'implicated': [1687], 'relay': [1689], 'mitogenic': [1691, 1767], 'stimuli': [1692], '(32Gingras': [1695], 'Kennedy': [1697], 'S.G.': [1698], "O'Leary": [1699], 'Hay': [1703], 'Genes': [1705], 'Dev.': [1706], '12:': [1708], '502-513Crossref': [1709], '(725)': [1712], 'Scholar,33Scott': [1714], 'P.H.': [1715, 2280], 'Kohn': [1718, 2283], 'A.D.': [1719, 2284], 'Roth': [1720, 2285], 'R.A.': [1721, 2286], '7772-7777Crossref': [1734, 2299], '(409)': [1737, 2302], 'whereas': [1740, 2818], 'presented': [1745, 4020, 4108], 'may': [1750, 2214, 2507, 4498], 'regulate': [1751], 'nutrient': [1753], '(amino': [1754], 'acid)-responsive': [1755], 'prerequisite,': [1760], 'but': [1761], 'not': [1762, 2445, 3520, 3564, 3747, 3778, 3867], 'overlap,': [1764], '(34Hara': [1769], 'Q.-P.': [1774], 'Belham': [1777], '273:': [1785, 2175, 4427, 4487], '14484-14494Abstract': [1786], '(1112)': [1794], 'Further': [1797], 'reconcile': [1802], 'observations.In': [1804], 'vitro': [1805, 2231, 2357], 'phosphorylations': [1806], 'both': [1808, 3474, 4473], 'reported': [1816], '(14Brunn': [1817], 'however,': [1868], 'definitive': [1869], 'still': [1872, 2305, 3558], 'needed': [1873], 'demonstrate': [1875], '(if': [1878], 'either)': [1879], 'substrate': [1883], '11-kDa': [1898], 'segment': [1899], 'located': [1900, 4458], 'N-terminal': [1901, 2759, 3399], '(35Chen': [1906, 1988, 2324, 2643, 3635, 4261], 'Zheng': [1908, 1990, 2326, 2645, 3637, 4263], 'X.F.': [1909, 1991, 2124, 2327, 2646, 3638, 4264], 'Brown': [1910, 1992, 2328, 2647, 3639, 3703, 4265], '4947-4951Crossref': [1923, 2005, 2341, 2660, 3652, 4278], '(445)': [1926, 2008, 2344, 2663, 3655, 4281], '36Chiu': [1929], 'M.I.': [1930], 'Katz': [1931], 'Berlin': [1933], 'V.': [1934], '12574-12578Crossref': [1944], '(408)': [1947], '(Fig.': [1950, 3404, 3487, 3817], '1).': [1951], 'serine': [1953, 1981, 2058], 'residue,': [1954], 'Ser2035,': [1955], 'within': [1956], 'FKBP12-rapamycin;': [1967], 'substitutions': [1968], 'site': [1971, 2511, 3509, 4180, 4250, 4515], 'any': [1973], 'larger': [1977, 3429, 4252], 'side': [1978, 4253], 'chains': [1979, 4254], 'than': [1980, 3430, 3834, 3856, 4406], 'abolish': [1982, 4255], 'formation': [1983, 4257], 'ternary': [1986, 4415], 'Consistently,': [2011], 'full-length': [2012, 2716, 4304], 'bearing': [2014], 'mutations': [2016, 2054, 2603, 2624, 2677, 4247, 4308], 'displays': [2017], 'resistance': [2019, 2068], 'Jurkat': [2021], 'Similarly,': [2044], 'yeast': [2047], 'homologues': [2048], 'TOR1': [2051], 'equivalent': [2057], 'residue': [2059, 4497], 'were': [2060, 2540, 2606, 2625, 2678, 2766, 2808, 2824, 2837, 2859, 2890, 2916, 2995, 3013, 3041, 3051, 3094, 3123, 3147, 3185, 3231, 3322, 3345, 3411, 3470, 3840, 3969, 3989, 3999, 4008, 4057, 4077, 4087, 4096], 'genetic': [2063], 'analysis': [2064, 2897], 'confer': [2066], '(37Cafferkey': [2069], 'Young': [2071], 'P.R.': [2072], 'McLaughlin': [2073], 'Koltin': [2077], 'Sathe': [2079], 'Eng': [2083], 'W.K.': [2084], '1993;': [2092, 3714], '13:': [2093], '6012-6023Crossref': [2094], '(256)': [2097], '38Helliwell': [2100], 'S.B.': [2101], 'Wagner': [2102], 'Kunz': [2104], 'Deuter-Reinhard': [2106], 'Henriquez': [2108], '105-118Crossref': [2117], '(314)': [2120], '39Zheng': [2123], 'Florentino': [2125], 'D.': [2126], '121-130Abstract': [2136], 'Crystal': [2145], 'structure': [2146, 3387, 4412], 'indicates': [2151], 'defined': [2154], 'four-helical': [2155], 'bundle': [2156], 'N': [2159], 'close': [2163], 'together': [2164], '(40Choi': [2165, 4417, 4477], 'Clardy': [2171, 4423, 4483], '239-242Crossref': [2176, 4428, 4488], '(710)': [2179, 4431, 4491], 'implying': [2182], 'potential': [2184, 3569], 'module.': [2189], 'rapamycin-inhibition': [2199], 'Although': [2205, 3541], 'common': [2210], 'speculation': [2211], 'inhibit': [2215, 2227], 'micromolar': [2221], 'concentrations': [2222, 2362, 3784, 3859], '33Scott': [2279], 'need': [2306], 'reconciled': [2309], 'low': [2312], 'IC50': [2313], 'observed': [2314, 3523, 3902, 4162], 'vivo(subnanomolar)': [2316], 'high': [2319, 3782], 'affinity': [2320, 3225], 'FKBP12-rapamycin-FRAP': [2322], 'Only': [2347], '15–50%': [2348], 'was': [2358, 2565, 2593, 2729, 2736, 2782, 2788, 2875, 2953, 2969, 3079, 3167, 3298, 3367, 3435, 3466, 3491, 3522, 3535, 3557, 3776, 3787, 3848, 3893, 3899, 4518, 4527], 'inhibited': [2359], 'nanomolar': [2361], '(30Burnett': [2365], 'bound': [2393], 'retain': [2399, 4402], 'robust': [2401], 'autokinase': [2402], '(41Brunn': [2404], 'Sabers': [2408], 'EMBO': [2417], '15:': [2420], '5256-5267Crossref': [2421], '(620)': [2424], 'absence': [2428], 'other': [2433, 4405, 4440], 'PIK-related': [2434], 'seems': [2437], 'imply': [2439, 4136], 'probably': [2444], 'intrinsic': [2449], 'catalytic': [2450, 3533, 3581], 'kinase.': [2454], 'To': [2455, 2577, 2714, 2904, 3334, 3383, 4204], 'understand': [2456], 'pathway,': [2467], 'probed': [2470], 'FRBin': [2475], 'vivo': [2476], 'examined': [2478, 3659], 'Here': [2490], 'report': [2492], 'experimental': [2493], 'suggesting': [2495, 3530, 3918], 'activator;': [2515], 'putative': [2517, 4505], 'necessary': [2522], 'Both': [2529], 'embryonic': [2536], 'kidney': [2537], '293': [2538], 'maintained': [2541], 'DMEM': [2543, 3054], '10%': [2545, 3056], 'serum': [2548, 3728, 3735], '(FBS)': [2549], 'penicillin/streptomycin': [2551], '37': [2553, 2827, 3061], '°C': [2554, 2963, 3062, 3303, 3361], '5%': [2556, 3064], 'CO2.': [2557], 'Escherichia': [2558], 'coli': [2559, 3634], 'strain': [2560], 'BL21': [2561, 2811], '(F−,': [2562], 'ompT,hsdSB(rB−mB+),dcm,': [2563], 'gal)': [2564], 'expression': [2568], 'isolation': [2570], 'GST-FRB': [2572, 2803], 'wild-type': [2573, 2804, 3931, 4403], 'mutant': [2575, 2638, 2806, 3409, 3933, 4398, 4525], 'express': [2578], 'GST': [2581, 2852], 'fusions,': [2582], 'cDNA': [2584, 2718], 'encoding': [2585], 'from': [2587, 2680, 2792, 3632], 'amino': [2588], 'acid': [2589], '2015': [2590], '2114': [2592], 'inserted': [2594, 2706], 'vector': [2597, 2710], 'pGEX-2T': [2598], '(Amersham': [2599, 2843], 'Pharmacia': [2600, 2844], 'Biotech).': [2601, 2845], 'Point': [2602], 'introduced': [2607, 2626, 2738], 'PCR': [2609], 'using': [2610, 2862, 3222], 'primers': [2611], 'carrying': [2612, 4306], 'desired': [2614], 'mutations:': [2615], 'Tyr2105': [2617], 'Ala': [2619, 3956], 'Trp2027': [2621, 2720, 4522], 'Phe': [2623, 3961], '3′': [2629, 2741], '5′': [2631, 2724, 2755, 2772], 'primers,': [2632], 'respectively.': [2633, 2778], 'Ser2035': [2635, 2670, 4307], 'Ile': [2637, 3951], 'described': [2641, 3973, 4061], 'previously': [2642, 3686], 'Full-length': [2666], 'cDNAs': [2668], 'Thr': [2672], 'Asp2357': [2674], 'Glu': [2676], 'isolated': [2679], 'corresponding': [2681], 'pBJ5': [2682], 'constructs': [2683], 'pCDNA3': [2709], 'NotI': [2712], 'sites.': [2713], 'construct': [2715, 2787, 4226], 'Phe,': [2722], 'end': [2725], '5.9-kilobase': [2726], 'pair': [2727, 2743], 'first': [2730], 'removed': [2731], 'byKpnI': [2732], 'digest;': [2733], 'then': [2737, 2954, 3124, 3368], '1755-base': [2742], 'fragment': [2744, 2781, 3426, 3443], 'ApaI': [2746], 'PflMI': [2748], 'sites,': [2749], 'followed': [2750, 2937, 3102, 3269, 3350], 're-insertion': [2752], 'KpnI': [2756], 'fragment.': [2757], 'mutants': [2761, 3401, 4441], '1362C,': [2762], '1967C,': [2763], '2061C': [2765], 'constructed': [2767], 'deleting': [2769], 'EcoRI,': [2774], 'KpnI,': [2775], 'andApaI': [2776], 'site,': [2777], '1819C': [2780, 3556], 'generated': [2783, 3395], 'PCR.': [2785], 'pGEX-4E-BP1': [2786], 'generous': [2790], 'gift': [2791], 'Dr.': [2793], 'Nahum': [2794], "Sonenberg's": [2795], 'laboratory': [2796], 'McGill': [2798], 'University.': [2799], 'expressions': [2801], 'induced': [2809, 2825], 'room': [2813], 'temperature': [2814], 'ensure': [2816], 'solubility,': [2817], 'GST-FKBP12': [2819, 2913], 'GST-4E-BP1': [2821, 3291], 'fusion': [2822, 2835], '°C.': [2828], 'Purification': [2829], 'thrombin': [2831], 'cleavage': [2832, 2847], 'carried': [2838, 3299, 3346, 3970, 3990, 4058, 4078], 'out': [2839, 3300, 3347, 3545, 3971, 3991, 4059, 4079, 4224], 'following': [2840, 4230], 'standard': [2841], 'procedures': [2842], 'After': [2846], 'removal': [2849], 'most': [2851], 'glutathione-Sepharose,': [2855], 'further': [2860], 'Sephacryl': [2864], 'S-100': [2865], 'column': [2866], '(2.5': [2867], 'cm': [2868], '×': [2869, 3153], '100': [2870, 3039, 3280], 'cm).': [2871], 'entire': [2873], 'procedure': [2874], 'performed': [2876], 'saline': [2879], '(PBS)': [2880], 'buffer': [2881, 2923, 2979, 2985, 3189, 3242, 3250], 'mm': [2884, 2927, 3025, 3191, 3197, 3200, 3203, 3206, 3209, 3215, 3218, 3256, 3264, 3267], 'DTT.': [2885], 'concentrated': [2891], '4.5–6': [2893], 'mg/ml,': [2894], 'SDS-PAGE': [2896, 3328], 'indicated': [2898, 3595, 3767, 3827, 3912], 'homogeneity': [2899], '98%.': [2903], 'assess': [2905, 3335], 'capacity': [2908], 'mutants,': [2912, 3553], 'pre-mixed': [2917], 'ml': [2920, 2976], '(PBS': [2924], 'DTT': [2928, 3026], '0.3%': [2930, 3211], 'Triton': [2931, 3107, 3212, 3261], 'X-100)': [2932], 'μm': [2935, 2942, 3281], 'each,': [2936], 'addition': [2939, 3271, 3312, 3352, 3455], 'brief': [2946], 'incubation': [2947, 3113], 'ice.': [2949], 'Glutathione-Sepharose': [2950], '(20': [2951, 3190], 'μl)': [2952], 'added': [2955, 3080], 'mixture': [2958, 3278], 'rocked': [2960], '4': [2962, 3115, 3360, 3857], 'h.': [2966, 3009, 3364], 'matrix': [2968, 3366], 'subsequently': [2970, 3136], 'washed': [2971, 3232, 3369], 'three': [2972, 3233], 'times': [2973, 3234, 3239], 'boiled': [2981], 'SDS': [2983, 2991, 3318, 3375], 'sample': [2984, 3319, 3376], 'loaded': [2987, 3326], '15%': [2990], 'gel.': [2992], 'plated': [2996], 'onto': [2997, 3149], 'coverslips': [2998, 3050], '∼30%': [3000], 'confluence': [3001], 'starved': [3003, 3011], 'serum-free': [3005], 'medium': [3006], '48': [3008], 'microinjected': [3014, 3755], 'various': [3016, 3880], '4.5–5': [3019], 'mg/ml': [3020, 3029, 3758, 3858], 'PBS': [3022], 'fluorescein-conjugated': [3030], 'dextran': [3031], '(M': [3032], 'r': [3033], '70,000;': [3034], 'Molecular': [3035], 'Probes).': [3036], 'injected': [3042, 3092, 3785, 3838, 3877, 3909, 4001, 4006, 4025, 4089, 4094, 4113], 'each': [3043, 3176, 3993, 4003, 4081, 4091], 'time.': [3044], 'Upon': [3045], 'completion': [3046], 'injection,': [3048], 'transferred': [3052], 'FBS': [3057], 'incubated': [3059, 3125], 'CO2for': [3065], '25': [3066, 3199, 3202, 3744], 'h': [3067, 3073, 3157, 3180, 3745], 'before': [3068, 3325], 'staining.': [3069], 'Two': [3070], '12': [3072], 'prior': [3074, 3158], 'fixation,': [3076], 'bromodeoxyuridine': [3077], '(BrdUrd)': [3078], 'media': [3083], 'final': [3086], 'concentration': [3087, 3804, 3888, 3920], 'μm.': [3090], 'fixed': [3095], '3.7%': [3097], 'formaldehyde': [3098], '15': [3100, 3307], 'min,': [3101], 'permeabilization': [3104], '0.1%': [3106, 3260], 'X-100': [3108], '10': [3110, 3119, 3263, 3283], 'min': [3111, 3134, 3308], 'n': [3116], 'HCl': [3117], 'min.': [3120], 'monoclonal': [3128], 'anti-BrdUrd': [3129], 'antibody': [3130, 3142], '(Sigma)': [3131], '30': [3133, 3302], 'rhodamine-conjugated': [3139], 'anti-mouse': [3140], 'IgG': [3141], '(Jackson': [3143], 'Immunoresearch).': [3144], 'HEK293': [3145, 3415], 'seeded': [3148], '60-mm': [3150], 'plates': [3151], '(1': [3152], '106': [3154], 'cells/plate)': [3155], '12–16': [3156], 'transfection.': [3160], 'Six': [3161], 'microliters': [3162], 'SuperFect': [3164], 'reagent': [3165], '(Qiagen)': [3166], 'transfect': [3170], '2': [3171, 3205, 3208, 3288, 3363], 'μg': [3172, 3289], 'plate.': [3177], '24': [3179], 'after': [3181], 'transfection,': [3182], 'lysed': [3186], 'lysis': [3188, 3236, 3241, 3344, 3371], 'sodium': [3192], 'phosphate,': [3193], 'pH': [3194, 3253], '7.2,': [3195], 'Na3VO4,': [3198], 'NaF,': [3201], 'β-glycerophosphate,': [3204], 'EDTA,': [3207], 'EGTA,': [3210], 'X-100,': [3213, 3262], 'phenylmethylsulfonyl': [3219], 'fluoride),': [3220], 'immunoprecipitated': [3221], 'M2': [3223], 'FLAG': [3224], '(Sigma).': [3227], 'immune': [3229], 'complexes': [3230], 'buffer,': [3237, 3372, 3377], '0.5m': [3244], 'NaCl,': [3245, 3257], 'once': [3247], '(40': [3251], 'mmTris-HCl,': [3252], '7.5,': [3254], '50': [3255], '20%': [3258], 'glycerol,': [3259], 'MnSO4,': [3265], 'DTT),': [3268], '20': [3273, 3314], 'μl': [3274, 3315], 'reaction': [3277, 3297], 'ATP,': [3282], 'μCi': [3284], '[γ-32P]ATP,': [3286], 'buffer.': [3295, 3320], 'rocking': [3305, 3358], 'stopped': [3310], '2×': [3317], 'Samples': [3321], 'heated': [3323], 'briefly': [3324], 'autoradiography': [3330], 'Western': [3332, 3381], 'analysis.': [3333], 'transfection': [3341], 'above,': [3349], 'ATP-agarose': [3354], '(C8-linked;': [3355], 'Sigma)': [3356], 'eluted': [3373], 'analyzed': [3379], 'blotting.': [3382], 'examine': [3384, 4228], 'primary': [3386], 'requirement': [3388], 'activity,': [3392], 'series': [3397], 'deletion': [3400, 3502, 3539], 'A).': [3406], 'These': [3407, 3566, 4134], 'truncated': [3408], 'transiently': [3412], 'expressed': [3413], 'cells,': [3416], 'immunoprecipitated,': [3417], 'assayed': [3419], 'their': [3421], 'autophosphorylation': [3422, 3497, 3505, 3508], 'activities.': [3423], 'Surprisingly,': [3424], '(1362C)': [3427], 'much': [3428], 'predicted': [3432], '(Fig.1': [3440], 'B).': [3441, 3489], 'This': [3442, 3774], 'includes': [3444], 'stretch': [3450], 'domain.': [3460], 'alone': [3464, 3809], '(2061C)': [3465], 'inactive,': [3467], 'so': [3469], 'fragments': [3472], 'domains': [3479], 'additional': [3481], '(1819C': [3484], '1967C)': [3486], 'It': [3490, 4284], 'conceivable': [3492], 'loss': [3495, 3515, 3527], 'might': [3498], 'due': [3500], 'site(s).': [3506], 'unknown.': [3512], 'phosphorylation': [3518], '(data': [3519, 3563, 3746, 3866], 'shown)': [3521], 'simultaneously': [3524], 'autophosphorylation,': [3529], 'abolished': [3536], 'mutants.': [3540, 4232], 'cannot': [3543], 'rule': [3544], 'possibility': [3547], 'misfolding': [3550], 'able': [3559], 'bind': [3561, 4531], 'shown).': [3565, 3748, 3868], 'implicate': [3568], 'involvement': [3570], 'and/or': [3573], 'regulation': [3577], 'activity.': [3582], 'obvious': [3585], 'significance': [3586, 3821], 'effect,': [3598], 'considering': [3600], 'implications': [3602], 'decided': [3609], 'investigate': [3611], 'more': [3618, 3833], 'approach.': [3620], 'Taking': [3621], 'advantage': [3622], 'large': [3625], 'quantities': [3626], 'soluble': [3628], 'E.': [3633], 'introducing': [3663], 'excess': [3664, 4191], 'exogenous': [3665], 'cultured': [3668], 'microinjection.': [3671], 'Human': [3672], 'line': [3676, 3698], 'chosen': [3679], 'experiments': [3682, 3988, 4076], 'because': [3683], 'demonstrated': [3687], 'characterized': [3692], '(42Albers': [3699], 'Tanaka': [3705], 'F.L.': [3708], '268:': [3715], '22825-22829Abstract': [3716], 'Cells': [3723], 'synchronized': [3724, 3966, 4054], 'G0': [3726], 'starvation': [3729], 're-entered': [3730], 'upon': [3734, 3928], 'stimulation': [3736], 'completed': [3738], 'phase': [3740, 4012, 4040, 4100, 4128], '(DNA': [3741], 'synthesis)': [3742], 'Fig.': [3752], '2,': [3753], '4.5': [3757], 'no': [3761, 3811], 'longer': [3762], 'entered': [3763], 'phase,': [3765], 'lack': [3770], 'synthesis.': [3773], 'apparently': [3777], 'artifact': [3780], 'specific': [3788], 'protein,': [3792], 'injection': [3794, 3850, 3874, 3994, 4036, 4082, 4124], 'nonspecific': [3797], '(rabbit': [3799], 'IgG)': [3800], 'indicator': [3807], 'fluorescein-dextran': [3808], 'had': [3810], '2).': [3818], 'statistical': [3820], 'data': [3829, 3923, 4018, 4106], 'summarized': [3830], 'TableI;': [3832], '80%': [3835], 'arrested': [3841, 3860], 'G1.': [3843], 'concentration-dependent;': [3849], 'less': [3855], 'lower': [3862, 3906], 'percentage': [3863, 4023, 4111], 'Due': [3869], 'variation': [3872], 'flow': [3875], 'rate,': [3876], 'received': [3879], 'amounts': [3881, 3907], 'even': [3884], 'when': [3885], 'constant': [3887], '(4.5': [3889], 'mg/ml)': [3890], 'used.': [3894], 'Normal': [3895], 'almost': [3900], 'always': [3901], '(as': [3911], 'fluorescence': [3915], 'intensity),': [3916], 'again': [3917], 'dependence.Table': [3921], 'IStatistical': [3922], 'microinjection': [3929], 'proteinsProtein': [3935], 'injectedPercentage': [3936], 'phase%No': [3941], 'injection∼97Dextran91.0': [3942], '±': [3943, 3945, 3948, 3953, 3958, 3963], '3.6IgG88.7': [3944], '6.1Wild-type': [3946], 'FRB16.2': [3947], '7.2Ser2035': [3949], 'FRB22.4': [3952], '5.0Tyr2105': [3954], 'FRB18.8': [3957], '6.0Trp2027': [3959], 'FRB86.1': [3962], '9.7Microinjections': [3964], 'under': [3974, 4041, 4062, 4129, 4516], '"Experimental': [3975, 4063], 'Procedures"': [3976, 4064], 'legends': [3979, 4067], 'Figs.': [3981, 4069], '2and': [3982, 4070], '4.': [3983, 4071], 'Three': [3984, 4072], 'eight': [3986, 4074], 'independent': [3987, 4075], 'condition,': [3995, 4083], '50–200': [3997, 4085], 'successfully': [4000, 4088], 'experiment.': [4004, 4092], 'All': [4005, 4093], 'scored': [4009, 4097], 'entry': [4013, 4101], 'BrdUrd': [4015, 4103], 'incorporation,': [4016, 4104], 'phase.': [4029, 4117], 'On': [4030, 4118], 'average,': [4031, 4119], 'about': [4032, 4120], '97%': [4033, 4121], 'growth': [4044, 4132], 'conditions.': [4045, 4133], 'Open': [4046], 'table': [4047], 'new': [4050], 'tab': [4051], 'Microinjections': [4052], 'free': [4139, 4193], 'acts': [4142], 'factor': [4147, 4198], 'normal': [4151, 4501], 'presumably': [4155], 'Assuming': [4160], 'specific,': [4166], 'one': [4167], 'simple': [4168], 'explanation': [4169], 'either': [4182], 'activator': [4185], 'effector;': [4189], 'titrates': [4195], 'off': [4196], 'therefore': [4200], 'confirm': [4205], 'specificity': [4207], 'characterize': [4216], 'structure-function': [4218], 'set': [4223], 'Serine': [4233], '2035': [4234], 'binding;': [4245], 'FKBP12-rapamycin-FRB': [4259, 4414], 'obviously': [4286], 'great': [4288], 'interest': [4289], 'mutated': [4298], 'Ser2035.': [4300], 'given': [4302], 'can': [4309], 'normally': [4315], 'expected': [4400], 'binding.': [4408, 4454, 4513], 'Using': [4409], 'crystal': [4411], 'guide,': [4436], 'designed': [4438], '(Fig.3': [4444], 'A)': [4445], 'could': [4447], 'potentially': [4448], 'interfere': [4449], 'presumed': [4452], 'effector/activator': [4453], 'Tyrosine': [4455], '2105': [4456], 'interface': [4461], 'forms': [4470], 'interactions': [4471], 'disrupt': [4499], 'if': [4503], 'effector': [4506], 'molecularly': [4509], 'similar': [4510], 'Another': [4514], 'consideration': [4517], 'tryptophan': [4519], '2027;': [4520], 'Arg': [4524], 'unable': [4529], 'rapamycin-FKBP12.': [4532]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W2000281583', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2023, 'cited_by_count': 7}, {'year': 2022, 'cited_by_count': 7}, {'year': 2021, 'cited_by_count': 7}, {'year': 2020, 'cited_by_count': 6}, {'year': 2019, 'cited_by_count': 5}, {'year': 2018, 'cited_by_count': 3}, {'year': 2017, 'cited_by_count': 4}, {'year': 2016, 'cited_by_count': 3}, {'year': 2015, 'cited_by_count': 8}, {'year': 2014, 'cited_by_count': 4}, {'year': 2013, 'cited_by_count': 9}, {'year': 2012, 'cited_by_count': 3}], 'updated_date': '2025-01-19T11:52:54.813384', 'created_date': '2016-06-24'}