Turnover of Heparan Sulfate Depends on 2-O-Sulfation of Uronic Acids

Name: Work Video:
Duration: 3 min 30 s
Xiaomei Bai; Karen J. Bame; Hiroko Habuchi; Koji Kimata; Jeffrey D. Esko
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4341], 'Chem.': [45, 291, 612, 666, 697, 713, 733, 747, 763, 781, 795, 833, 884, 903, 920, 944, 960, 983, 1024, 1042, 1082, 1100, 1128, 1223, 1321, 1783, 2150, 2170, 2406, 2509, 2947, 3046, 3095, 3472, 3490, 3506, 3541, 3606, 3761, 3807, 3825, 3880, 3942, 3980, 4092, 4342], '271,': [46, 292], '17711–17717).': [47, 293], 'Heparan': [48, 294], 'from': [50, 193, 208, 296, 439, 454, 1448, 1477, 1534, 1764, 1798, 2186, 2333, 2348, 2395, 2768, 2908, 2973, 3079, 3368, 3388, 3413, 3664, 4111, 4186, 4219, 4284, 4385, 4437], 'contains': [53, 299, 2963], 'normal': [54, 176, 300, 422, 3710, 4016], 'amounts': [55, 301, 4384], '6-O-sulfated': [57, 303, 3713, 4066], 'glucosamine': [58, 69, 304, 315, 1188, 1203, 3562, 3714, 3724, 4067], 'iduronic': [61, 75, 224, 307, 321, 470, 1412, 3717, 3745, 4059], 'somewhat': [64, 310, 3720], 'higher': [65, 311, 3397, 3721], 'levels': [66, 312, 3711, 3722], 'N-sulfated': [68, 314, 1202], 'but': [71, 237, 317, 483, 1429, 4389, 4505], 'lacks': [72, 318], 'any': [73, 319, 3743], '2-O-sulfated': [74, 223, 320, 469, 1467, 3744, 4058], 'or': [76, 105, 322, 351, 505, 599, 689, 1212, 3220, 3438, 3521, 4065, 4176, 4289], 'glucuronic': [77, 323], 'residues.': [79, 325, 1414, 3702, 3913, 4068, 4156], 'Pulse-chase': [80, 326], 'experiments': [81, 327, 1335, 4259], 'showed': [82, 328, 3786, 4118], 'that': [83, 222, 329, 468, 685, 1458, 1563, 2910, 3061, 3787, 3843, 3902, 3968, 3996, 4119, 4136, 4301], 'both': [84, 330, 4120, 4366, 4397], 'transport': [89, 335], 'newly': [90, 336, 4427], 'synthesized': [91, 337], 'proteoglycans': [94, 340, 497, 515, 591, 806, 1676, 1707, 2339, 2563, 2767, 2886, 3427, 3445, 4281, 4326, 4429, 4434], 'to': [95, 118, 136, 142, 183, 214, 341, 364, 382, 388, 429, 460, 682, 1200, 1276, 1454, 1596, 1729, 1816, 1866, 1871, 1876, 2001, 2010, 2105, 2290, 2372, 2641, 2752, 2764, 2798, 2904, 2991, 3011, 3461, 3570, 3741, 3749, 3965, 4148, 4162], 'plasma': [97, 343, 3379], 'membrane,': [98, 344], 'where': [99, 345], 'they': [100, 346], 'shed': [101, 347, 3433, 4290], 'into': [102, 107, 348, 353, 2247, 2981, 3245, 3434], 'medium': [104, 350, 1506, 1531, 1634, 1662, 1750, 2665, 2672, 3000, 3437, 4287], 'move': [106, 352], 'cell': [109, 355, 588, 1406, 1625, 1808, 2226, 2335, 2677, 3265, 3693, 3970, 4026, 4041, 4295, 4305, 4324, 4439], 'through': [110, 356, 595, 3288], 'endocytosis.': [111, 357, 3442], 'Internalization': [112, 358], 'cell-associated': [115, 361], 'molecules': [116, 362], 'leads': [117, 363], 'sequential': [119, 365, 627], 'endoglycosidase': [120, 366], '(heparanase)': [121, 367], 'fragmentation': [122, 368], 'chains': [125, 134, 168, 173, 200, 207, 371, 380, 414, 419, 446, 453, 1453, 1795, 2004, 2042, 2495, 3410, 3689, 3708, 3781, 3842, 4110, 4125, 4142, 4216, 4321, 4379, 4391, 4454, 4469, 4473, 4501], 'eventual': [127, 373], 'lysosomal': [128, 374, 4177], 'degradation.': [129, 375], 'In': [130, 376, 1641, 3736, 3835, 4441], 'cells,': [132, 378, 3681, 4188, 4190], 'begin': [135, 381], 'degrade': [137, 383, 818], 'within': [138, 384], '1': [139, 385, 1602, 1637, 1839, 1913, 1998, 2212, 2257, 2260, 2268, 2630, 2694, 2716, 2722, 2738, 2789, 3246, 3270, 3275, 3279, 3283, 4268], 'h,': [140, 386, 3119], 'leading': [141, 387], 'accumulation': [144, 390, 3449], 'intermediate': [146, 392, 826, 1436, 3454, 3530, 4121, 4450, 4497], '(10–20-kDa)': [147, 393, 3455], 'small': [149, 395, 2761, 3457, 3532, 4081, 4123, 4447, 4452, 4483, 4499], '(4–7-kDa)': [150, 396, 3458], 'oligosaccharides.': [151, 397], 'Mutant': [152, 398], 'did': [154, 400], 'not': [155, 181, 238, 401, 427, 484, 1421, 1752, 2502], 'generate': [156, 402], 'these': [157, 403, 2490, 3529], 'intermediates,': [158, 404], 'although': [159, 405], 'internalization': [160, 406], 'intracellular': [162, 408, 1719, 3451, 4299, 4400], 'trafficking': [163, 409], 'appeared': [169, 415], 'normal,': [170, 416], 'degraded': [174, 420], 'with': [175, 421, 1116, 1336, 1511, 1604, 1636, 1672, 1683, 1736, 1823, 1864, 1890, 1897, 1912, 1925, 1949, 1974, 1993, 2023, 2120, 2193, 2202, 2236, 2243, 2344, 2450, 2468, 2600, 2629, 2646, 2652, 2683, 2693, 2775, 2785, 2802, 2821, 2834, 2862, 2914, 3033, 3069, 3113, 3147, 3157, 3172, 3208, 3215, 3448, 3672, 3709, 3798, 3922, 4195, 4265, 4303, 4426, 4479, 4514], 'kinetics.': [177, 423], 'This': [178, 424, 2961, 3703], 'difference': [179, 425], 'was': [180, 426, 1532, 1639, 1647, 1663, 1895, 1937, 1954, 1964, 1985, 2017, 2065, 2118, 2189, 2299, 2312, 2342, 2361, 2378, 2391, 2425, 2460, 2466, 2555, 2673, 2728, 2757, 2773, 2796, 2811, 2853, 2860, 2878, 2926, 2978, 3124, 3153, 3197, 3318, 3336, 3381, 4004, 4146, 4255, 4420, 4510], 'due': [182, 428, 3748, 4147], 'heparanase': [185, 431, 637, 1441, 4174], 'activities': [186, 432], 'mutant,': [189, 435], 'since': [190, 436, 1758], 'cytoplasmic': [191, 437], 'extracts': [192, 438, 3078, 4051], 'cleaved': [196, 442], 'vitro.': [202, 448], 'Instead,': [203, 449], 'were': [211, 457, 1475, 1488, 1594, 1627, 1668, 1677, 1695, 1708, 1715, 1727, 1796, 1814, 1854, 1861, 1910, 2021, 2028, 2043, 2103, 2215, 2232, 2241, 2273, 2525, 2541, 2564, 2587, 2620, 2639, 2679, 2750, 2815, 2827, 2868, 2901, 2911, 2996, 3009, 3064, 3082, 3110, 3143, 3170, 3223, 3252, 3267, 3296, 3366, 3393, 3411, 3920, 3958, 4000, 4076, 4126, 4159, 4206, 4217, 4263, 4282, 4327, 4380], 'relatively': [212, 458], 'resistant': [213, 459], 'degradation': [215, 461, 630, 647, 1391, 3447, 3463, 4168, 4522], 'by': [216, 234, 462, 480, 600, 648, 1679, 1966, 2030, 2045, 2380, 2543, 2557, 2832, 2870, 2929, 3066, 3128, 3199, 3221, 3298, 3402, 3418, 3441, 3782, 3960, 4114, 4227, 4257, 4524], 'cellular': [217, 463, 1455, 3523], 'heparanases.': [218, 464], 'These': [219, 465, 1194, 3555, 4133, 4460], 'findings': [220, 466, 4134], 'suggest': [221, 467], 'are': [230, 476, 3431], 'important': [231, 477], 'for': [232, 239, 478, 485, 1205, 1278, 1557, 1601, 1687, 1701, 1990, 2191, 2275, 2287, 2305, 2365, 2427, 2486, 2531, 2626, 2666, 2690, 2734, 2855, 2916, 3084, 3117, 3217, 3258, 3306, 3348, 4166, 4208, 4267, 4272], 'cleavage': [233, 479, 638, 1215, 2499, 4492, 4509], 'endogenous': [235, 481], 'heparanases': [236, 482, 654, 816, 3524], 'overall': [241, 487, 1424], 'chains.': [245, 491], 'Animal': [492], 'elaborate': [494], 'several': [495, 626, 683], 'membrane-associated': [496], 'bearing': [498], 'sulfate,': [500, 1428, 1523], 'including': [501, 631, 852], 'betaglycan': [502], 'one': [504, 4003], 'more': [506, 3522], 'members': [507], 'syndecan': [510], 'glypican': [512], 'families': [513], '(1Esko': [516], 'J.D.': [517, 1772, 3015, 3043, 3092, 3758, 3977, 4089], 'Curr.': [518], 'Opin.': [519], 'Cell': [520, 561, 1674, 2561, 3020, 3077, 3189, 3425], '1991;': [522, 537], '3:': [523, 1179], '805-816Crossref': [524], 'PubMed': [525, 540, 566, 578, 619, 675, 704, 720, 740, 754, 770, 788, 802, 840, 869, 891, 907, 927, 951, 967, 990, 1008, 1031, 1049, 1091, 1107, 1137, 1156, 1181, 1232, 1251, 1266, 1302, 1328, 1350, 1377, 1549, 1582, 1790, 2159, 2179, 2415, 2518, 2580, 2956, 3055, 3104, 3481, 3497, 3515, 3550, 3587, 3613, 3626, 3653, 3770, 3816, 3832, 3889, 3951, 3989, 4101, 4351], 'Scopus': [526, 541, 567, 579, 676, 870, 908, 1009, 1092, 1138, 1157, 1182, 1233, 1252, 1267, 1303, 1351, 1378, 1550, 1583, 2160, 2180, 2416, 2519, 2581, 2957, 3056, 3105, 3482, 3516, 3551, 3588, 3627, 3654, 3771, 3817, 3890, 3952, 3990, 4102, 4352], '(183)': [527], 'Google': [528, 543, 569, 581, 620, 678, 705, 721, 741, 755, 771, 789, 803, 841, 872, 892, 910, 928, 952, 968, 991, 1011, 1032, 1050, 1094, 1108, 1140, 1159, 1184, 1235, 1254, 1269, 1305, 1329, 1353, 1380, 1552, 1585, 1791, 2162, 2182, 2418, 2521, 2583, 2959, 3058, 3107, 3484, 3498, 3518, 3553, 3590, 3614, 3629, 3656, 3773, 3819, 3833, 3892, 3954, 3992, 4104, 4354], 'Scholar,': [529, 544, 570, 706, 722, 742, 756, 772, 790, 873, 929, 1095, 1141, 1160, 1236, 1255, 1306, 1354, 2163, 3485, 3499, 3591, 3615, 3630, 3820], '2Kjellén': [530], 'L.': [531, 724, 931, 1015, 1308, 1360, 3183, 3593, 3636], 'Lindahl': [532, 916, 940, 1317, 3602], 'U.': [533, 896, 917, 941, 1318, 1543, 3603], 'Annu.': [534, 559], 'Rev.': [535, 560], 'Biochem.': [536, 1001], '60:': [538], '443-475Crossref': [539], '(1678)': [542], '3Bernfield': [545], 'M.': [546, 550, 607, 708, 730, 744, 758, 776, 792, 830, 864, 973, 996, 1021, 1039, 1151, 1164, 1246, 1259, 1297, 1343, 1575, 2936, 2940, 3582, 3619, 3931, 3935], 'Kokenyesi': [547], 'R.': [548, 1573], 'Kato': [549], 'Hinkes': [551], 'M.T.': [552], 'Spring': [553], 'Gallo': [555], 'R.L.': [556], 'Lose': [557], 'E.J.': [558], '1992;': [563, 613, 782, 796, 834], '8:': [564], '365-393Crossref': [565], '(968)': [568], '4David': [571], 'G.': [572, 933, 1310, 1529, 2571, 3595], 'FASEB': [573], '1993;': [575, 698, 1101, 1178, 3491, 3826], '7:': [576], '1023-1030Crossref': [577], '(374)': [580], 'Scholar).': [582, 621, 804, 842, 1109, 1270, 1381, 1586, 1792, 2183, 2419, 2522, 2584, 2960, 3059, 3108, 3194, 3519, 3554, 3657, 3774, 3834, 3893, 3955, 3993, 4105], 'After': [583, 1619, 1856, 2353, 2669], 'their': [584, 1119, 1191, 2011, 3903], 'appearance': [585], 'on': [586, 1282, 2384, 2590, 2759, 2898, 3338, 3859, 3907, 4323], 'surface,': [589], 'turn': [592], 'over': [593, 2211], 'either': [594, 3432], 'shedding': [597], 'process': [598], 'endocytosis': [601], '(as': [602], 'reviewed': [603], 'Ref.': [605], '5Yanagishita': [606], 'Hascall': [608, 709, 759, 777], 'V.C.': [609, 710, 760, 778], '267:': [614, 783, 797, 835], '9451-9454Abstract': [615], 'Full': [616, 670, 672, 701, 717, 737, 751, 767, 785, 799, 837, 888, 924, 948, 964, 987, 1028, 1046, 1086, 1088, 1104, 1132, 1134, 1227, 1229, 1325, 1787, 2154, 2156, 2174, 2176, 2410, 2412, 2513, 2515, 2951, 2953, 3050, 3052, 3099, 3101, 3476, 3478, 3494, 3510, 3512, 3545, 3547, 3610, 3765, 3767, 3811, 3813, 3829, 3884, 3886, 3946, 3948, 3984, 3986, 4096, 4098, 4346, 4348], 'Text': [617, 671, 673, 702, 718, 738, 752, 768, 786, 800, 838, 889, 925, 949, 965, 988, 1029, 1047, 1087, 1089, 1105, 1133, 1135, 1228, 1230, 1326, 1788, 2155, 2157, 2175, 2177, 2411, 2413, 2514, 2516, 2952, 2954, 3051, 3053, 3100, 3102, 3477, 3479, 3495, 3511, 3513, 3546, 3548, 3611, 3766, 3768, 3812, 3814, 3830, 3885, 3887, 3947, 3949, 3985, 3987, 4097, 4099, 4347, 4349], 'PDF': [618, 674, 703, 719, 739, 753, 769, 787, 801, 839, 890, 926, 950, 966, 989, 1030, 1048, 1090, 1106, 1136, 1231, 1327, 1789, 2158, 2178, 2414, 2517, 2955, 3054, 3103, 3480, 3496, 3514, 3549, 3612, 3769, 3815, 3831, 3888, 3950, 3988, 4100, 4350], 'The': [622, 653, 1443, 1486, 1693, 1713, 1744, 1805, 1893, 1935, 1951, 1981, 2039, 2063, 2116, 2184, 2239, 2271, 2296, 2376, 2523, 2547, 2585, 2618, 2637, 2726, 2755, 2771, 2813, 2824, 2865, 2922, 2976, 3195, 3250, 3264, 3376, 3391, 3443, 3894, 4069, 4250, 4318, 4399, 4471], 'endocytic': [623], 'pathway': [624], 'involves': [625], 'steps': [628], 'proteolysis': [632], 'core': [635], 'protein,': [636], 'chains,': [643, 1757, 3566, 3795, 4423], 'eventually': [645], 'complete': [646, 3462], 'proteases,': [649], 'exoglycosidases,': [650], 'sulfatases.': [652], 'cleave': [655], 'infrequently': [656], '(6Bame': [660, 1076, 1122, 1217, 2144, 3466, 3535, 3801], 'K.J.': [661, 694, 1077, 1097, 1123, 1218, 2145, 2167, 2403, 2506, 3467, 3487, 3503, 3536, 3802, 3822, 3877, 4339], 'Robson': [662, 1078, 1124, 1219, 2146, 3468, 3537, 3803], 'K.': [663, 899, 1079, 1125, 1220, 1358, 2147, 2933, 2944, 3469, 3538, 3634, 3804, 3939], '1997;': [667, 1083, 1129, 1224, 2151, 2171, 2407, 2510, 2948, 3191, 3473, 3507, 3542, 3808, 3881, 3943, 4343], '272:': [668, 1084, 1130, 1225, 2152, 2172, 2408, 2511, 2949, 3474, 3508, 3543, 3809, 3882, 3944, 4344], '2245-2251Abstract': [669, 1085, 1131, 1226, 2153, 3475, 3544, 3810], '(28)': [677, 1093, 1139, 1234, 2161, 3483, 3552, 3818], 'Scholar),': [679, 893, 911, 953, 969, 992, 1012, 1033, 1051, 1553], 'giving': [680], 'rise': [681], 'fragments': [684, 828, 2130, 2500, 3534, 4461], 'accumulate': [686], 'lysosomes': [688, 821, 3392, 3465], 'possibly': [690], 'prelysosomal': [691], 'compartments': [692], '(7Bame': [693], '268:': [699, 1102, 3492, 3827], '19956-19964Abstract': [700, 1103, 3493, 3828], '8Yanagishita': [707], '1984;': [714, 984], '259:': [715, 985], '10270-10283Abstract': [716], '9Kjellén': [723], 'Pertoft': [725, 1016], 'H.': [726, 1017, 2930, 2938, 3933], 'Oldberg': [727, 999, 1018], 'Å.': [728, 856, 935, 1000, 1019, 1143, 1238, 1289, 1312, 3574, 3597], 'Hook': [729, 1020], '1985;': [734, 748, 764, 1025, 1043], '260:': [735, 749, 765, 1026, 1044], '8416-8422Abstract': [736, 1027], '10Yanagishita': [743], '11075-11082Abstract': [750, 1045], '11Yanagishita': [757], '5445-5455Abstract': [766], '12Takeuchi': [773], 'Y.': [774, 1174], 'Yanagishita': [775], '14677-14684Abstract': [784], '13Yanagishita': [791], '9505-9511Abstract': [798, 836], 'Some': [805, 4496], '(e.g.': [807, 4170], 'those': [808], 'containing': [809, 1466, 1838, 1904, 1997, 2074, 2254, 2603, 2698, 2887, 3001, 3236, 3274, 3325], 'glycosylphosphatidylinositol': [811], 'anchor)': [812], 'may': [813, 1208], 'bypass': [814], 'rapidly': [819, 4361], 'without': [822], 'formation': [824, 1434, 3527, 3904], 'sized': [827, 4124, 4453, 4500], '(13Yanagishita': [829], 'Heparanases': [843], 'have': [844, 1400], 'been': [845, 1565], 'found': [846, 3394, 4322], 'various': [848, 1799], 'tissues,': [851], 'human': [853], 'platelets': [854], '(14Oldberg': [855, 1288, 3573], 'Heldin': [857, 1144, 1239, 1290, 3575], 'C.-H.': [858, 1145, 1240, 1291, 3576], 'Wasteson': [859, 934, 997, 1146, 1241, 1292, 1311, 3577, 3596], 'A.': [860, 998, 1147, 1176, 1242, 1293, 1545, 1774, 2575, 3578], 'Busch': [861, 1148, 1243, 1294, 3579], 'C.': [862, 1149, 1172, 1244, 1295, 3580], 'Höök': [863, 1150, 1245, 1296, 3581], 'Biochemistry.': [865, 1152, 1247, 1262, 1298, 1346, 3583, 3622], '1980;': [866, 1153, 1248, 1299, 3584], '19:': [867, 1154, 1249, 1300, 3585], '5755-5762Crossref': [868, 1155, 1250, 1301, 3586], '(78)': [871, 1158, 1253, 1304, 3589], '15Oosta': [874], 'G.M.': [875], 'Favreau': [876], 'L.V.': [877], 'Beeler': [878], 'D.L.': [879], 'Rosenberg': [880], 'R.D': [881], '1982;': [885, 945, 1322, 3607], '257:': [886, 946, 1323, 3608], '11249-11255Abstract': [887], 'placenta': [894], '(16Klein': [895], 'Von': [897], 'Figura': [898], 'Hoppe-Seylers.': [900], 'Z.': [901], 'Physiol.': [902], '1979;': [904], '360:': [905], '1465-1471Crossref': [906], '(17)': [909], 'mouse': [912, 970], 'mastocytoma': [913], '(17Ogren': [914], 'S.': [915, 939, 1166, 1316, 1544, 2165, 2401, 2504, 3501, 3601, 3875, 4337], '1975;': [921, 1005], '250:': [922], '2690-2697Abstract': [923], '18Thunberg': [930, 1307, 3592], 'Bäckström': [932, 1309, 3594], 'Robinson': [936, 1313, 3598], 'H.C.': [937, 1314, 3599], 'Ögren': [938, 1315, 3600], '10278-10282Abstract': [947, 1324, 3609], 'colon': [954], 'carcinoma': [955], '(19Iozzo': [956], 'R.V.': [957], '1987;': [961], '262:': [962], '1888-1900Abstract': [963], 'melanoma': [971], '(20Nakajima': [972], 'Irimura': [974], 'T.': [975, 1257, 1341, 1776, 3617], 'Di': [976], 'Ferrante': [977], 'N.': [978, 1364, 3640], 'Nicolson': [979, 1260, 1344, 3620], 'G.L.': [980, 1261, 1345, 3621], '2283-2290Abstract': [986], 'rat': [993, 1034], 'liver': [994], '(21Höök': [995], 'Biophys.': [1002], 'Res.': [1003], 'Commun.': [1004], '67:': [1006], '1422-1428Crossref': [1007], '(65)': [1010], 'hepatocytes': [1013], '(9Kjellén': [1014], 'ovarian': [1035], 'granulosa': [1036], '(10Yanagishita': [1038], 'CHO': [1053, 1462, 1589, 1722, 1759, 2974, 3429, 3692, 3928], '1The': [1054], 'abbreviations': [1055], 'used': [1056, 2104], 'are:': [1057], 'CHO,': [1058], 'ovary;': [1061], 'HPLC,': [1062], 'high': [1063], 'performance': [1064], 'liquid': [1065, 2544], 'chromatography;': [1066], 'bFGF,': [1067], 'basic': [1068, 3035], 'fibroblast': [1069, 3036], 'growth': [1070, 1505, 2999, 3037, 3436, 4223, 4286, 4387], 'factor;': [1071], 'PBS,': [1072], 'phosphate-buffered': [1073, 1569], 'saline.': [1074], '7Bame': [1096, 3486, 3821], 'As': [1110], 'endo-β-glucuronidases,': [1111], 'enzymes': [1113, 1195, 1460, 3556, 3671], 'produce': [1114], 'oligosaccharides': [1115, 3452], 'GlcUA': [1117], 'at': [1118, 1190, 1211, 1690, 1697, 1930, 1971, 1987, 2085, 2283, 2301, 2356, 2368, 2430, 2483, 2527, 2597, 2623, 2687, 2730, 2817, 3226, 3254, 3261, 3302, 3309, 3351, 3354], 'reducing': [1120], 'ends': [1121], '14Oldberg': [1142, 1237], '22Brandley': [1161], 'B.K.': [1162], 'Kiso': [1163], 'Abbas': [1165], 'Nikrad': [1167], 'P.': [1168], 'Srivasatava': [1169], 'O.': [1170, 2942, 3937], 'Foxall': [1171], 'Oda': [1173], 'Hasegawa': [1175], 'Glycobiology.': [1177, 1373, 2576, 3649], '633-639Crossref': [1180], '(208)': [1183], 'Scholar)': [1185, 1330, 3030, 4355], 'residue': [1189], 'nonreducing': [1192], 'ends.': [1193], 'generally': [1196], 'require': [1197, 3558], 'substrate': [1199], 'contain': [1201, 3790], 'activity,': [1206], 'which': [1207, 4261], 'be': [1209, 3383, 3571], 'located': [1210], 'near': [1213, 1597, 3845], 'site': [1216], '23Irimura': [1256, 3616], 'Nakajima': [1258, 1342, 3618], '1986;': [1263, 1347, 1784, 3623], '25:': [1264, 1348, 3624], '5322-5328Crossref': [1265, 1349, 3625], '(136)': [1268, 1352, 3628], 'A': [1271, 1829, 2743, 2792, 3329, 4373], 'less': [1272], 'stringent': [1273], 'requirement': [1274], 'appears': [1275], 'exist': [1277], 'O-sulfate': [1279], 'groups': [1280, 3568], 'based': [1281], 'utilization': [1284], 'different': [1286, 4273], 'substrates': [1287, 1465], 'vitro': [1333, 3666], 'competition': [1334], 'chemically': [1337], 'modified': [1338], 'heparins': [1339], '(23Irimura': [1340], '24Lapierre': [1355, 3631], 'F.': [1356, 3632], 'Holme': [1357, 3633], 'Lam': [1359, 3635], 'Tressler': [1361, 3637], 'R.J.': [1362, 1368, 3638, 3644], 'Storm': [1363, 3639], 'Wee': [1365, 3641], 'Stack': [1367, 3643], 'Castellot': [1369, 3645], 'Tyrrell': [1371, 3647], 'D.J.': [1372, 3648], '1996;': [1374, 3047, 3096, 3650, 3762, 3981, 4093], '6:': [1375, 3651], '355-366Crossref': [1376, 3652], '(139)': [1379, 3655], 'might': [1389], 'affect': [1390, 1422], 'vivo,': [1398], 'turnover': [1402, 1425, 3685, 4251], 'line': [1407], 'Altered': [1415], 'does': [1420, 1751], 'defect': [1431, 4169], 'prevents': [1432], 'breakdown': [1437], 'products': [1438, 2867, 4047, 4071], 'characteristic': [1439], 'action.': [1442], 'lack': [1444, 1745, 3895], 'intermediates': [1446, 3897, 4523], 'results': [1447], 'reduced': [1449, 4512], 'susceptibility': [1450], 'heparanases,': [1456], 'suggesting': [1457, 4489], 'prefer': [1464], 'acids.': [1469], '(CHO-K1)': [1474], 'obtained': [1476, 3297, 4185], 'American': [1479], 'Type': [1480], 'Culture': [1481], 'Collection': [1482], '(CCL-61,': [1483], 'Rockville,': [1484], 'MD).': [1485], 'grown': [1489, 1595], 'under': [1490], 'an': [1491, 2194, 2873], 'atmosphere': [1492], '5%': [1494], 'CO2,': [1495], '95%': [1496], 'air': [1497], '100%': [1499], 'relative': [1500, 2903], 'humidity': [1501], "Ham's": [1503], 'F-12': [1504, 1617, 1633, 1742, 2654], '(Life': [1507, 2987], 'Technologies,': [1508, 2988], 'Inc.)': [1509, 2989, 3317], 'supplemented': [1510, 1635], '7.5%': [1512], '(v/v)': [1513], 'fetal': [1514, 1560], 'bovine': [1515, 1561, 2747], 'serum': [1516, 1562, 2748], '(HyClone': [1517], 'Laboratories),': [1518], '100': [1519, 1525, 1605, 1644, 2068, 2320, 2442], 'μg/ml': [1520, 1645, 2258, 2261, 2717, 2746, 3003, 3276, 3280], 'streptomycin': [1522], 'units/ml': [1526], 'penicillin': [1528], 'Sulfate-free': [1530], 'prepared': [1533, 1882, 2347, 3319], 'individual': [1535], 'components': [1536], '(25Ham': [1537], 'R.G.': [1538], 'Proc.': [1539], 'Natl.': [1540], 'Acad.': [1541], 'Sci.': [1542, 3190], '1965;': [1546], '53:': [1547], '288-293Crossref': [1548], '(645)': [1551], 'substituting': [1554], 'chloride': [1555, 2472], 'salts': [1556], 'had': [1564], 'dialyzed': [1566], 'exhaustively': [1567], 'against': [1568], 'saline': [1570], '(PBS)': [1571], '(26Dulbecco': [1572], 'Vogt': [1574], 'Exp.': [1577], 'Med.': [1578], '1954;': [1579], '99:': [1580], '167-182Crossref': [1581], '(2000)': [1584], 'Both': [1587], 'pgsF-17': [1592, 1725, 2983, 3141, 3212, 4044, 4240], 'confluence': [1598], 'pulse-labeled': [1600], 'h': [1603, 1735, 1992, 2429, 2918, 3219, 4269], 'μCi/ml': [1606, 3115], 'Na35SO4': [1607, 1737], '(25–40': [1608], 'Ci/mg;': [1609], 'NEN': [1610], 'Life': [1611, 2100], 'Science': [1612, 2101], 'Products)': [1613, 2102], 'free': [1616], 'medium.': [1618, 1652, 1743], 'removing': [1620], 'labeled': [1622, 1728, 2658, 2913, 3112, 3214, 3778, 4207, 4264], 'medium,': [1623, 1806, 2655, 4388], 'layers': [1626, 2678], 'washed': [1628, 1669, 1896, 2233, 2594, 2644, 2680, 2774, 2829, 3144, 3224], 'three': [1629, 1670, 2234, 2681, 2830, 3145, 3164, 3206], 'times,': [1630], 'fresh': [1632], 'mmNa2SO4': [1638], 'added.': [1640, 1855, 2812], 'some': [1642], 'experiments,': [1643], 'chloroquine': [1646], 'included': [1648], 'chase': [1651, 4364, 4465], 'At': [1653, 4410], 'times': [1655, 1671, 2235, 2596, 2682, 2831, 3146, 3207], 'indicated': [1656, 3867, 3901], 'figure': [1659], 'legends,': [1660], 'collected,': [1664], 'PBS.': [1673, 2238, 3148, 3209], 'surface': [1675, 1809, 2336, 2562, 3426, 3971, 4296, 4325], 'released': [1678, 1706], 'treating': [1680, 1967], '0.125%': [1684], '(w/v)': [1685, 2076, 2470], 'trypsin': [1686, 4308], '5–10': [1688], 'min': [1689, 2289, 2367, 2628, 2692, 3260, 3308, 3350], '37': [1691, 1972, 2357, 2431, 2484], '°C.': [1692, 3263, 3311], 'centrifuged': [1696, 2282, 2300, 2364, 2526, 2729, 3253, 3347], '800': [1698, 3255, 3303], '×': [1699, 2056, 2285, 2303, 2350, 2370, 2529, 2732, 3256, 3304, 3872, 4334], 'g': [1700, 2286, 2304, 2371, 2530, 2733, 3257, 3305], '3': [1702, 2139, 4456, 4494], 'min,': [1703, 2307, 2533, 2736], 'recovered': [1709, 2897, 2907, 4381], 'supernatant.': [1712, 2754], 'pellets': [1714, 3266], 'designated': [1716], 'as': [1717, 2398, 2566, 2880, 3087, 3132, 3400, 3422, 4466, 4503], 'pool.': [1720], 'Wild-type': [1721, 3210], 'constant': [1730], 'radiospecific': [1731], 'activity': [1732, 2390, 3086, 4020], 'after': [1733, 4307], '24': [1734, 1991, 2428, 2667, 2917, 3218], '(50': [1738], 'μCi/ml)': [1739, 2661], 'sulfate-free': [1741, 2653, 2663], 'cause': [1753, 4165], 'undersulfation': [1754], 'derive': [1761], 'adequate': [1762], 'cysteine': [1768], 'methionine': [1770], '(27Esko': [1771], 'Elgavish': [1773], 'Prasthofer': [1775], 'Taylor': [1777], 'W.H.': [1778], 'Weinke': [1779], 'J.L.': [1780], '261:': [1785], '15725-15733Abstract': [1786], 'Radiolabeled': [1793], 'glycosaminoglycan': [1794], 'isolated': [1797, 2332, 3125, 3412, 4218, 4283], 'fractions': [1800, 3415], 'following': [1803], 'way.': [1804], 'pool,': [1810], 'pellet': [1813, 2311, 2346, 4306], 'adjusted': [1815], '0.1': [1817], 'm': [1818, 1846, 1873, 1906, 1914, 1995, 1999, 2081, 2475, 2609, 2777, 2790, 2800, 2849, 3327], 'NaOH': [1819, 1996], 'then': [1821, 2593, 2657, 2828, 2920], 'neutralized': [1822], '10m': [1824], 'acetic': [1825], 'acid.': [1826], 'Chondroitin': [1827], '(2': [1830, 1933], 'mg)': [1831], '16volume': [1833], 'protease': [1836], 'solution': [1837, 2602, 3345], 'mg/ml': [1840, 2631], 'Pronase': [1841], '(Boehringer': [1842], 'Mannheim)': [1843], '0.24': [1845], 'sodium': [1847, 1919, 1941, 2322, 2439, 2444, 2479], 'acetate': [1848, 1900, 1920, 1942, 2480], '(pH': [1849, 1902, 1921, 1960, 2072, 2252, 2324, 2446, 2481, 2606, 2705, 2845, 3234, 3323], '6.5)': [1850], '1.92m': [1852], 'NaCl': [1853, 1915, 2082, 2778, 2801, 3239], 'overnight': [1857, 1970, 2354, 2820], 'incubation,': [1858], 'solutions': [1860], 'diluted': [1862, 2022, 2797], '5-fold': [1863], 'water': [1865], 'reduce': [1867, 2006], 'salt': [1869], 'concentration': [1870], '∼0.1': [1872], 'applied': [1875], '0.5-ml': [1878], 'column': [1879, 1894, 2053, 2064, 2117, 2188, 2763, 2772, 3853, 3863], 'DEAE-Sephacel': [1881, 2762], 'disposable': [1885], 'polypropylene': [1886], 'pipette': [1887], 'tip': [1888], 'plugged': [1889], 'glass': [1891, 3070], 'wool.': [1892], '20': [1898, 1917, 1957, 1975, 3158, 3230], 'mmsodium': [1899], 'buffer': [1901, 2071, 2251, 2316, 2435, 2648, 2697, 2782, 2836, 3233, 3273], '6.0)': [1903, 1922, 2073], '0.25': [1905], 'NaCl.': [1907, 2328, 2791, 2850, 3328], 'Bound': [1908], 'glycosaminoglycans': [1909, 2027], 'eluted': [1911, 2784, 3844], 'mm': [1918, 1958, 2055, 2069, 2249, 2265, 2321, 2438, 2443, 2478, 2612, 2616, 2703, 2708, 2711, 2714, 2723, 2843, 3231, 3238], 'precipitated': [1924, 2374], '4': [1926, 1931, 1988, 2598, 2624, 2688, 2818, 3227, 3262, 3310, 3352], 'volumes': [1927], 'ethanol': [1929, 2037], '°C': [1932, 1973, 1989, 2432, 2485, 2599, 2625, 2689, 2819, 3228, 3353], 'h).': [1934], 'precipitate': [1936], 'dissolved': [1938, 1955], '0.5m': [1940], '(1': [1943, 3334, 3364], 'ml,': [1944], 'pH': [1945], '5.5)': [1946, 2482], 'reprecipitated': [1948], 'ethanol.': [1950], 'final': [1952], 'material': [1953, 4291], 'Tris-HCl': [1959, 2250, 2844], '7.4).': [1961], '[35S]chondroitin': [1962], 'removed': [1965, 2869], 'sample': [1969, 2360, 2756, 2793, 2859, 3330], 'milliunits': [1976], 'chondroitinase': [1978, 2863], 'ABC': [1979], '(Seikagaku).': [1980], 'remaining': [1982], 'treated': [1986, 2467], '0.5': [1994, 2080, 2090, 2799, 2848], 'NaBH4': [2000], 'β-eliminate': [2002], 'terminal': [2008], 'sugars': [2009], 'corresponding': [2012], 'alditols.': [2013], 'Carrier': [2014, 2456], 'chondroitin': [2015, 2122, 2741], 'added,': [2018, 2461], 'samples': [2020, 2524, 3301], 'water,': [2024, 2803], 'purified': [2029, 2393, 3670], 'another': [2031, 2858], 'round': [2032], 'DEAE': [2034], 'chromatography': [2035], 'precipitation.': [2038], '[35S]heparan': [2040, 2330, 2421, 3122, 3779, 4108, 4183, 4214, 4253], 'analyzed': [2044, 2379, 3127, 3417, 4226], 'gel': [2046, 2381, 2558, 3129, 3419, 3783, 4115, 4228], 'filtration': [2047, 2382, 2559, 3130, 3420, 3784, 4116, 4180, 4229], 'HPLC': [2048, 2383, 3131, 3421, 3785, 4117, 4181, 4230], 'using': [2049, 2217, 2985, 3357], 'TSK': [2051, 2386, 3851, 3861], 'G2000SW': [2052, 2387], '(7.5': [2054], '30': [2057, 2627, 2805], 'cm': [2058], 'column;': [2059], 'TosoHaas,': [2060], 'Montgomeryville,': [2061], 'PA).': [2062], 'equilibrated': [2066], 'KH2PO4': [2070], '0.2%': [2075], 'Zwittergent': [2077], '3–12': [2078], 'run': [2084], 'flow': [2087], 'rate': [2088], 'ml/min': [2091], '(0.5-ml': [2092], 'fractions).': [2093], 'Blue': [2094], 'dextran': [2095], '(Sigma)': [2096, 2810], '[6-3H]glucosamine-HCl': [2098], '(NEN': [2099], 'determine': [2106, 4163], 'V': [2108], 'o': [2109], 'andV': [2110], 't': [2111], 'column,': [2114], 'respectively.': [2115], 'calibrated': [2119], 'standard': [2121], '(∼50': [2124], 'kDa,K': [2125, 2132], 'av': [2126, 2133, 2142, 3865], '∼0.13)': [2127], 'heparin': [2129, 2457], '(13.5': [2131], '∼0.4;': [2134], '5–6': [2135], 'kDa,': [2136, 2140], 'K': [2137, 2141], 'av∼0.6;': [2138], '∼0.8)': [2143], '28Tumova': [2164, 3500], 'Bame': [2166, 2402, 2505, 3502, 3876, 4338], '9078-9085Abstract': [2173, 2409, 2512, 3509, 3883, 4345], '(27)': [2181, 2417, 2520, 3517, 3891, 4353], 'effluent': [2185], 'monitored': [2190], 'radioactivity': [2192, 2196], 'in-line': [2195], 'detector': [2197], '(Radiomatic': [2198], 'FLO-ONE/Beta,': [2199], 'Packard': [2200], 'Instruments)': [2201], 'sampling': [2203], 'rates': [2204], 'every': [2205], '6': [2206], 's': [2207, 2277], 'data': [2209], 'averaged': [2210], 'min.': [2213], 'Samples': [2214], 'counted': [2216, 2879], 'Ultima': [2218], 'Gold': [2219], 'XR': [2220], 'scintillation': [2221, 2545], 'fluid': [2222], '(Packard).': [2223], 'prepare': [2225], 'extracts,': [2227], 'confluent': [2228], 'monolayers': [2229, 3136], 'cold': [2237], 'scraped': [2242, 3244], 'rubber': [2245], 'policeman': [2246], '50': [2248, 2318, 2437, 2745, 2842, 3114, 3349], '7.4)': [2253, 3235], '0.25m': [2255], 'sucrose,': [2256], 'leupeptin,': [2259, 3281], 'pepstatin': [2262, 2719, 3277], 'A,': [2263, 2720, 3278], '10': [2264, 2276, 2288, 2306, 2366, 2532, 2604, 2611, 2615, 2691, 2702, 2710, 2713, 3292, 3307, 3321, 4023, 4031], 'N-ethylmaleimide,': [2266, 2715], 'mmphenylmethlsulfonyl': [2269, 3284], 'fluoride.': [2270, 2725], 'sonicated': [2274], '(on': [2278], '50%': [2279], 'duty)': [2280], '1000': [2284, 2528, 2731], 'remove': [2291, 2373], 'nuclei': [2292], 'unbroken': [2294], 'cells.': [2295, 2352, 2975, 4398, 4409], 'postnuclear': [2297, 3332], 'supernatant': [2298, 2377, 2540, 3333], '14,000': [2302, 2369], 'microsomal': [2310], 'resuspended': [2313, 3268], 'mmcitrate,': [2319], 'phosphate': [2323, 2445], '5.5),': [2325], '0.15m': [2327], 'Purified': [2329, 2420], 'pool': [2337, 4401], '(∼5000': [2340, 2423], 'cpm)': [2341, 2424], 'mixed': [2343], '∼5': [2349], '106': [2351], 'incubation': [2355], '°C,': [2358], 'boiled': [2362], 'proteins.': [2375, 2770, 3390], 'column.': [2388], 'Heparanase': [2389], 'partially': [2392, 3669], '(∼100-fold)': [2394], 'CHO-K1': [2396], 'described': [2399, 2567, 3088, 3133, 3423], '(28Tumova': [2400, 2503, 3874, 4336], 'incubated': [2426, 2686, 2816, 3032], 'citrate': [2440], '5.5,': [2447], '75': [2448], 'μl)': [2449], 'enzyme': [2451], '(1–1.5': [2452], 'μg': [2453], 'protein).': [2455], '(100': [2458, 2660], 'μg)': [2459], 'reaction': [2464, 3196], 'mixture': [2465], '1%': [2469], 'cetylpyridinium': [2471], '0.32': [2474], 'NaCl,': [2476, 2610, 2709], '40': [2477], '2': [2487, 3241, 4209], 'h.': [2488, 2668], 'Under': [2489], 'conditions,': [2491], 'uncleaved': [2492], 'precipitate,': [2496], 'whereas': [2497], 'do': [2501], '35S': [2536], 'counts': [2537, 2896, 2906, 3387], 'assayed': [2542, 3083], 'counting.': [2546], 'size': [2548, 3459, 3533, 4330, 4395], 'heparanase-cleaved': [2551], 'also': [2554, 3111, 4477], 'HPLC.': [2560], 'biotinylated': [2565], '(29Fransson': [2568], 'L.Å.': [2569], 'Edgren': [2570], 'Havsmark': [2572], 'B.': [2573], 'Schmidtchen': [2574], '1995;': [2577], '5:': [2578], '407-415Crossref': [2579], '(32)': [2582], 'first': [2588, 2912], 'chilled': [2589], 'ice': [2591], 'four': [2595], 'mmKH2PO4': [2605], '7.0),': [2607], '0.137': [2608], 'MgCl2,': [2613], 'EDTA.': [2617], 'gently': [2621], 'stirred': [2622], 'Sulfo-NHS-Biotin': [2633], '(Pierce)': [2634], 'buffer.': [2636, 3249], 'brought': [2640], 'room': [2642], 'temperature,': [2643], 'once': [2645], 'warm': [2647], '(37': [2649], '°C),': [2650], 'twice': [2651, 3225], 'with35SO4': [2659], 'F12': [2664], 'biotinylation,': [2670], 'removed,': [2674], 'PBS': [2684, 3156], 'ml': [2695, 2787, 3247, 3271, 3342], '2%': [2699], 'Triton': [2700, 2840], 'X-100,': [2701, 2841], 'K2HPO4': [2704], '7.4),': [2706, 2846], '150': [2707, 3237], 'EDTA,': [2712], 'phenylmethlsulfonyl': [2724], 'extract': [2727], '5': [2735, 3259, 4038], 'mg': [2739], 'albumin': [2749], 'added': [2751, 3154, 3171], 'chromatographed': [2758], 'separate': [2765], 'other': [2769], '0.3': [2776], 'same': [2781], '2.5': [2786], '(0.5': [2794, 3151], 'ml)': [2795, 3335, 3365], 'μl': [2806], 'strepavidin-agarose': [2808], 'beads': [2809, 2826, 2877, 2900], 'mixtures': [2814], 'end-over-end': [2822], 'mixing.': [2823], 'agarose': [2825], 'centrifugation': [2833, 4314], 'composed': [2837], '0.1%': [2839, 3168], 'An': [2851], 'aliquot': [2852, 2874], 'taken': [2854, 3439], 'counting,': [2856], 'digested': [2861], 'ABC.': [2864], 'digestion': [2866], 'centrifugation,': [2871], 'measure': [2882], 'biotin.': [2888], 'calculate': [2890], 'extent': [2892, 4507], 'recycling,': [2894], 'expressed': [2902], '35SO4': [2915, 3216, 4266], 'biotinylated.': [2921], '2-O-sulfotransferase': [2923, 2972, 3085, 3754, 3929, 4019], 'clone,': [2924], 'pcDNA3HS2ST,': [2925], 'kindly': [2927], 'provided': [2928], 'Habuchi': [2931, 2937, 2941, 3932, 3936], 'Kimata': [2934, 2943, 3938], '(30Kobayashi': [2935, 3930], 'Yoneda': [2939, 3934], '13980-13985Abstract': [2950, 3945], '(126)': [2958, 3953], 'clone': [2962, 3925], '1.28-kilobase': [2965], 'pair': [2966], 'open': [2967], 'reading': [2968], 'frame': [2969], 'encoding': [2970, 3926, 4198], 'plasmid': [2977], 'stably': [2979], 'transfected': [2980, 3921, 4194, 4204, 4239], 'Lipofectin': [2986], 'according': [2990], "manufacturer's": [2993], 'instructions.': [2994], 'Transfectants': [2995], 'selected': [2997, 3959], '400': [3002], 'G418': [3004], '(effective': [3005], 'concentration).': [3006], 'Resistant': [3007], 'colonies': [3008, 3967, 3995], 'replica-plated': [3010], 'polyester': [3012], 'cloth': [3013], '(31Esko': [3014], 'Tartakoff': [3016], 'A.M.': [3017], 'Methods': [3018], 'Biology:': [3021], 'Vesicular': [3022], 'Transport.': [3023], 'Academic': [3024], 'Press,': [3025], 'Inc.,': [3026], 'San': [3027], 'Diego1989:': [3028], '387-422Google': [3029], '125I-labeled': [3034], 'factor': [3038], '(bFGF)': [3039], '(32Bai': [3040, 3089, 3755, 3974, 4086], 'X.': [3041, 3090, 3756, 3975, 4087], 'Esko': [3042, 3091, 3757, 3976, 4088], '271:': [3048, 3097, 3763, 3982, 4094], '17711-17717Abstract': [3049, 3098, 3764, 3983, 4095], '(120)': [3057, 3106, 3772, 3991, 4103], 'Colonies': [3060], 'bound': [3062, 3997], 'bFGF': [3063, 3998], 'detected': [3065], 'autoradiography,': [3067], 'picked': [3068], 'cloning': [3071], 'cylinders,': [3072], 'expanded': [3074], 'culture.': [3076], 'transfectants': [3081, 3957], 'Cells': [3109], 'of35SO4': [3116], '8': [3118], 'above.': [3134, 3424], 'Confluent': [3135], 'Sodium': [3149], '[125I]iodide': [3150], 'mCi)': [3152], 'units': [3159], 'lactoperoxidase': [3161], '(Sigma),': [3162], '20-μl': [3165], 'aliquots': [3166], 'H2O2': [3169], 'gentle': [3173], 'agitation': [3174], '5-min': [3176], 'intervals': [3177], '(33Czekay': [3178], 'R.P.': [3179], 'Orlando': [3180], 'R.A.': [3181], 'Woodward': [3182], 'Adamson': [3184], 'E.D.': [3185], 'Farquhar': [3186], 'M.G.': [3187], '108:': [3192], '1433-1441Google': [3193], 'stopped': [3198], 'sedimenting': [3200], 'washing': [3204], 'them': [3205], 'radioiodination': [3222], 'HEPES': [3232], 'mmCaCl2': [3242], 'fluoride': [3285], 'forced': [3287], '28-gauge': [3290], 'needle': [3291], 'times.': [3293], 'Postnuclear': [3294], 'supernatants': [3295], 'centrifuging': [3299], 'Percoll': [3312, 3344], '(20%,': [3313], 'w/v)': [3314], '(Pharmacia': [3315], 'Biotech': [3316], 'mmTris-HCl': [3322], '8.0)': [3324], '0.15': [3326], 'loaded': [3337], 'top': [3339, 3370], '11': [3341], '20,000': [3355], '×g': [3356], '75Ti': [3359], 'fixed-angle': [3360], 'rotor': [3361], '(Beckman).': [3362], 'Fractions': [3363], 'collected': [3367], 'gradient': [3373], 'counted.': [3375], 'distribution': [3377, 3404], 'membranes': [3380], 'determined': [3382], 'recovery': [3385], 'of125I': [3386], 'surface-labeled': [3389], 'density': [3398], 'fractions,': [3399], 'assessed': [3401], 'β-hexosaminidase': [3406], 'activity.': [3407], '[35S]Heparan': [3408, 4279], 'pooled': [3414], 'up': [3440], 'internalized': [3444, 4436], 'undergo': [3446], 'prior': [3460], 'One': [3520], 'catalyze': [3525], 'apparently': [3557], 'presence': [3560], 'ofN-sulfated': [3561, 3723], 'butO-sulfate': [3567], 'appear': [3569], 'dispensable': [3572], 'However,': [3658], 'much': [3659], 'evidence': [3662], 'derives': [3663], 'studies': [3667, 3800, 4158], 'defined': [3673], 'substrates.': [3674], 'test': [3676], 'this': [3677, 3916], 'hypothesis': [3678], 'directly': [3679], '(pgsF-17)': [3695], 'produces': [3705], '(60%': [3726], 'GlcNSO3': [3727, 4064, 4078], 'versus': [3731, 4028], '45%': [3732], 'wild-type).': [3735], 'contrast,': [3737, 3836], 'fails': [3740], 'make': [3742], 'deficiency': [3751], 'Analysis': [3775, 4106], 'steady-state': [3777], 'large,': [3791], 'intermediate,': [3792], 'short': [3794], 'agreement': [3797, 4527], 'previous': [3799], 'contained': [3839, 4014, 4445], 'only': [3840], 'large': [3841, 4328, 4393, 4422, 4468, 4472], 'void': [3847], 'volume': [3848], '2000': [3852], '(Fig.': [3854, 4131, 4277, 4371, 4455, 4493], '1).': [3855], 'Their': [3856], 'elution': [3857, 4486], 'position': [3858, 4487], '4000': [3862], '(K': [3864], '∼0.4)': [3866], 'anM': [3868], 'r': [3869, 4332], '∼8': [3871, 4333], '104': [3873], 'somehow': [3905], 'depended': [3906], 'confirm': [3915], 'hypothesis,': [3917], 'cDNA': [3924, 4197], 'Stable': [3956], 'replica': [3961], 'plating': [3962], 'using125I-bFGF': [3963], 'blotting': [3964], 'detect': [3966], 'produced': [3969, 4520], 'Several': [3994], 'strongly': [3999], 'purified,': [4001], 'characterized': [4005], 'greater': [4007], 'detail.': [4008], 'By': [4009], 'enzymatic': [4010], 'assay,': [4011], 'transfectant': [4013, 4113, 4130], 'level': [4017], '(118': [4021], '±': [4022, 4030, 4037], 'pmol/min/mg': [4024, 4039], 'protein': [4027, 4042], '98': [4029], '15': [4036], 'cells).': [4045], '2The': [4046], 'generated': [4048], 'crude': [4050], 'consisted': [4055], '∼85%': [4057], '15%': [4063], 'sulfated': [4070], 'made': [4072, 4428], 'entirely': [4077], 'amount': [4082, 4359, 4448], '6-O-sulfate': [4084], 'esters': [4085], 'present': [4127], '2).': [4132], 'confirmed': [4135], 'altered': [4138, 4171], 'processing': [4139], 'reduction': [4149], 'Further': [4157], 'therefore': [4160], 'performed': [4161], 'secretion,': [4172], 'endocytosis,': [4173], 'cleavage,': [4175], 'degradation).Figure': [4178], '2Gel': [4179], '2-O-sulfotransferase.': [4200], 'Mutant,': [4201], 'wild-type,': [4202], 'days': [4210], 'with35SO4,': [4211], 'media': [4224], '(see': [4231, 4315], '"Experimental': [4232, 4316], 'Procedures").': [4233, 4317], '•,': [4234], 'wild-type;': [4235], '○,': [4236], 'pgsF-17;': [4237], '▵,': [4238], 'cells.View': [4241], 'Large': [4242], 'Image': [4243], 'Figure': [4244], 'ViewerDownload': [4245], 'Hi-res': [4246], 'image': [4247], 'Download': [4248], '(PPT)': [4249], 'measured': [4256], 'pulse-chase': [4258], 'chased': [4271], 'periods': [4274], 'time': [4276], '3).': [4278], '(secreted': [4288], 'solution),': [4293], '(trypsin-releasable),': [4297], 'pools': [4300], 'remained': [4302, 4392], 'treatment': [4309], '(M': [4331], '104)': [4335], 'decreased': [4357], 'very': [4360, 4403], 'during': [4362, 4463], '3,': [4372], 'B).': [4375], 'During': [4376], 'chase,': [4378], 'increasing': [4383], 'behaved': [4402], 'differently': [4404], 'start': [4412], 'experiment,': [4415], 'most': [4416], 'label': [4419], 'presumably': [4424], 'associated': [4425], 'Golgi': [4432], 'recently': [4435], 'surface.': [4440], 'addition,': [4442], 'E,': [4457], 'filled': [4458], 'circles).': [4459], 'accumulated': [4462, 4502], 'decreased.': [4470], 'declined': [4478], 'time,': [4480], 'shift': [4484], 'occurred,': [4488], 'slow,': [4490], 'incomplete': [4491], 'F).': [4495], 'well,': [4504], 'greatly': [4511], 'compared': [4513], 'wild-type.': [4516], 'Thus,': [4517], 'few': [4521], 'pulse-chase,': [4525]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1999966734', 'counts_by_year': [{'year': 2023, 'cited_by_count': 1}, {'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 4}, {'year': 2019, 'cited_by_count': 2}, {'year': 2018, 'cited_by_count': 1}, {'year': 2014, 'cited_by_count': 3}, {'year': 2013, 'cited_by_count': 1}], 'updated_date': '2024-12-15T08:05:38.063757', 'created_date': '2016-06-24'}
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