Yeast Sml1, a Protein Inhibitor of Ribonucleotide Reductase

Name: Work Video:
Duration: 3 min 30 s
Andrei Chabes; Vladimir Domkin; Lars Thelander
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'https://openalex.org/W1986764834', 'https://openalex.org/W1976181487', 'https://openalex.org/W1531601525'], 'abstract_inverted_index': {'Ribonucleotide': [0, 199, 405], 'reductase': [1, 61, 100, 169, 182, 200, 260, 299, 368, 381, 399, 406, 437, 693, 838, 1047, 1102, 2096, 2989, 3415], '(RNR)': [2, 201], 'catalyzes': [3, 202], 'the': [4, 41, 45, 50, 56, 85, 89, 131, 146, 166, 173, 188, 203, 240, 244, 249, 255, 284, 288, 330, 345, 365, 372, 387, 417, 427, 435, 443, 477, 489, 562, 637, 644, 653, 656, 687, 843, 846, 871, 876, 887, 909, 912, 926, 987, 1000, 1005, 1025, 1030, 1064, 1079, 1085, 1093, 1109, 1119, 1155, 1172, 1195, 1204, 1321, 1345, 1362, 1367, 1375, 1418, 1443, 1458, 1480, 1486, 1508, 1514, 1538, 1545, 1618, 1656, 1678, 1706, 1710, 1717, 1791, 1802, 1832, 1848, 1912, 1930, 1952, 1973, 2041, 2113, 2180, 2188, 2203, 2209, 2213, 2230, 2241, 2260, 2268, 2276, 2290, 2294, 2316, 2336, 2344, 2351, 2364, 2403, 2422, 2425, 2432, 2451, 2461, 2469, 2473, 2478, 2490, 2495, 2508, 2513, 2528, 2542, 2545, 2558, 2561, 2566, 2571, 2587, 2590, 2622, 2626, 2634, 2641, 2645, 2665, 2713, 2731, 2760, 2816, 2823, 2829, 2894, 2918, 2926, 2946, 2952, 2966, 2968, 2997, 3008, 3013, 3024, 3041, 3047, 3050, 3061, 3078, 3088, 3097, 3100, 3108, 3156, 3165, 3189, 3192, 3196, 3211, 3217, 3237, 3245, 3269, 3273, 3279, 3300, 3306, 3322, 3328, 3341, 3368, 3385, 3427, 3433, 3499, 3518, 3565, 3587, 3595, 3608, 3613, 3616, 3624, 3628, 3637, 3641, 3664, 3668, 3689, 3715, 3718, 3726, 3737, 3743, 3753, 3761, 3769, 3778, 3792, 3807, 3817, 3825, 3831, 3841, 3848, 3852, 3866, 3876, 3879, 3884, 3898, 3903, 3917, 3924, 3929, 3991, 4009], 'reduction': [5, 204, 419, 2104], 'of': [6, 20, 40, 59, 68, 88, 138, 157, 205, 219, 239, 258, 267, 287, 337, 356, 420, 447, 513, 530, 564, 636, 842, 845, 870, 878, 921, 989, 1016, 1057, 1133, 1194, 1203, 1404, 1519, 1550, 1629, 1638, 1648, 1698, 1709, 1779, 1786, 1793, 1810, 1842, 1845, 1847, 1888, 1916, 1919, 1925, 1941, 1954, 1965, 1970, 1997, 2004, 2011, 2019, 2087, 2090, 2115, 2118, 2126, 2145, 2156, 2166, 2175, 2202, 2212, 2243, 2247, 2263, 2282, 2297, 2305, 2315, 2325, 2341, 2350, 2424, 2442, 2446, 2450, 2475, 2485, 2492, 2512, 2521, 2531, 2544, 2552, 2604, 2617, 2625, 2633, 2648, 2657, 2662, 2671, 2676, 2682, 2693, 2702, 2705, 2717, 2723, 2741, 2763, 2779, 2788, 2806, 2825, 2842, 2851, 2865, 2871, 2879, 2890, 2903, 2912, 2938, 2981, 2999, 3015, 3019, 3028, 3033, 3040, 3087, 3122, 3174, 3195, 3210, 3220, 3239, 3343, 3376, 3413, 3426, 3432, 3498, 3536, 3550, 3573, 3615, 3717, 3794, 3816, 3916, 3931, 3943], 'ribonucleotides': [7, 206, 423], 'to': [8, 52, 82, 84, 97, 127, 145, 165, 207, 251, 281, 283, 296, 326, 344, 364, 424, 434, 519, 536, 560, 572, 652, 686, 997, 1024, 1063, 1084, 1128, 1137, 1400, 1467, 1502, 1513, 1805, 1831, 1838, 2185, 2192, 2208, 2223, 2229, 2239, 2274, 2293, 2343, 2357, 2363, 2402, 2502, 2570, 2608, 2644, 2664, 2687, 2699, 2735, 2859, 2875, 2945, 3046, 3063, 3095, 3202, 3291, 3297, 3320, 3437, 3564, 3646, 3682, 3760, 3776, 3847, 3878, 3897, 3923, 3982], 'deoxyribonucleotides;': [9, 208], 'this': [10, 93, 209, 292, 441, 604, 923, 1011, 2168, 3053, 3139, 3266, 3379], 'step': [11, 210], 'is': [12, 105, 134, 184, 211, 304, 333, 383, 509, 526, 599, 606, 641, 915, 1051, 1104, 1829, 2355, 2465, 2733, 2820, 2943, 3055, 3073, 3372, 3380, 3530, 3544, 3619, 3692, 3789, 3820, 3869, 3887], 'rate-limiting': [13, 212], 'in': [14, 49, 74, 149, 176, 178, 190, 213, 248, 273, 348, 375, 377, 389, 412, 440, 694, 934, 977, 1041, 1043, 1096, 1098, 1108, 1121, 1149, 1167, 1284, 1374, 1388, 1425, 1438, 1457, 1479, 1507, 1688, 1885, 1911, 1972, 1986, 2061, 2112, 2255, 2269, 2421, 2460, 2472, 2494, 2557, 2744, 2921, 2992, 2996, 3012, 3023, 3180, 3223, 3312, 3336, 3384, 3403, 3417, 3507, 3540, 3556, 3577, 3667, 3698, 3824, 3830, 3865, 3883, 3902, 3938], 'DNA': [15, 53, 103, 214, 252, 302, 413, 848, 1319, 1381, 3445], 'precursor': [16, 215], 'synthesis.': [17, 216], 'A': [18, 76, 217, 275, 2123], 'number': [19, 218], 'regulatory': [21, 220], 'mechanisms': [22, 36, 221, 235], 'ensure': [23, 222], 'optimal': [24, 223], 'deoxyribonucleotide': [25, 224, 3442, 3554], 'pools,': [26, 225], 'which': [27, 226, 598, 1112, 2354, 2685, 2708, 2748, 2942, 3952], 'are': [28, 37, 227, 236, 474, 732, 756, 840, 2221, 3035, 3740, 3980], 'essential': [29, 228, 600, 642], 'for': [30, 229, 499, 601, 643, 942, 1453, 1617, 1716, 1790, 1951, 1980, 2016, 2099, 2183, 2335, 2361, 3523, 3640, 3845, 3857, 3988, 3994, 4006], 'cell': [31, 46, 230, 245, 573], 'viability.': [32, 231], 'The': [33, 141, 232, 340, 483, 591, 632, 835, 1139, 1263, 1371, 1475, 1614, 1636, 1660, 1683, 1742, 1771, 1825, 1957, 1995, 2081, 2143, 2199, 2440, 2547, 2669, 2784, 2979, 3559, 3721], 'best': [34, 233, 2191], 'studied': [35, 234, 1673, 1774], 'transcriptional': [38, 237, 3424], 'regulation': [39, 58, 70, 238, 257, 269, 1018, 3512], 'RNR': [42, 69, 241, 268, 602, 873, 914, 1017, 2225, 2251, 2272, 2338, 2414, 3010, 3090, 3386, 3428, 3439, 3491, 3529, 3551, 3670, 3701, 3827, 3861], 'genes': [43, 242, 734, 759, 839, 874, 3429], 'during': [44, 243], 'cycle': [47, 246, 850], 'and': [48, 55, 112, 192, 247, 254, 311, 391, 429, 451, 479, 496, 646, 649, 683, 739, 931, 938, 979, 1008, 1145, 1163, 1198, 1202, 1266, 1271, 1280, 1333, 1354, 1434, 1543, 1554, 1664, 1766, 1782, 1834, 1905, 1936, 1978, 2002, 2079, 2134, 2160, 2163, 2179, 2233, 2284, 2328, 2411, 2419, 2448, 2453, 2497, 2533, 2636, 2652, 2847, 2907, 3022, 3058, 3077, 3104, 3116, 3310, 3396, 3419, 3430, 3441, 3553, 3576, 3591, 3633, 3675, 3725, 3736, 3829, 3840, 3859, 3872, 3890, 3921, 3958, 3985, 3993, 4002], 'response': [51, 250, 2778, 2817, 3221], 'damage,': [54, 253], 'allosteric': [57, 256, 505, 2426, 3234], 'ribonucleotide': [60, 91, 99, 168, 181, 259, 290, 298, 367, 380, 398, 431, 436, 692, 837, 1046, 1101, 2095, 2313, 2988, 3377, 3414], 'by': [62, 261, 415, 476, 511, 528, 608, 885, 918, 1069, 1312, 1380, 1393, 1410, 1422, 1436, 1462, 1536, 1674, 1800, 2040, 2227, 2515, 3091, 3144, 3261, 3493, 3513, 3533, 3768, 3784, 3791, 3803], 'nucleoside': [63, 262, 493, 500], 'triphosphates.': [64, 263], 'Recently,': [65, 264, 944], 'another': [66, 265], 'mode': [67, 266, 1015], 'has': [71, 270, 3393], 'been': [72, 271, 3394, 3680], 'hypothesized': [73, 272], 'yeast.': [75, 274, 935, 1122], 'novel': [77, 276, 970, 1014], 'protein,': [78, 277, 655, 1208, 3021, 3501, 3610, 3900, 3919], 'Sml1,': [79, 278], 'was': [80, 95, 279, 294, 995, 1019, 1209, 1310, 1350, 1359, 1378, 1386, 1445, 1477, 1488, 1622, 1641, 1672, 1686, 1702, 1798, 1883, 2000, 2068, 2083, 2097, 2332, 2555, 2584, 2594, 2792, 2801, 2976, 3110, 3126, 3137, 3141, 3200, 3259, 3598, 3644, 3749, 3782, 3836], 'shown': [81, 280, 996, 1068, 2254, 2471], 'bind': [83, 282, 2569, 3759], 'Rnr1': [86, 285, 1002, 1026, 1556, 3213, 3567, 3723], 'protein': [87, 133, 286, 332, 485, 593, 659, 689, 971, 1003, 1027, 1088, 1506, 1671, 1685, 1720, 2036, 2067, 2120, 2232, 2554, 2643, 2732, 2983, 3080, 3103, 3125, 3177, 3199, 3248, 3282, 3287, 3302, 3371, 3435, 3520, 3561, 3568, 3691, 3724, 3729, 3755, 3809, 3844, 3895, 3908, 3926], 'yeast': [90, 139, 147, 191, 289, 338, 346, 390, 428, 836, 872, 913, 1001, 1045, 1058, 1065, 1110, 1141, 1199, 1264, 1317, 1384, 1555, 1666, 1796, 2094, 2250, 2271, 3051, 3212, 3274, 3369, 3418, 3528, 3557, 3566, 3669, 3700, 3722, 3858, 3867], 'reductase;': [92, 291, 3378], 'interaction': [94, 293, 1661, 1792, 3618, 3626, 3666], 'proposed': [96, 295], 'inhibit': [98, 297, 691, 1029, 2224, 2413], 'activity': [101, 300, 525, 538, 2021, 2100, 2226, 3011, 3416, 3440, 3492, 3702], 'when': [102, 301, 2794, 2974], 'synthesis': [104, 303], 'not': [106, 305, 916, 2171, 2197, 2488, 2613, 2620, 2810, 2964, 3082, 3185, 3531, 3585, 3678], 'required': [107, 306], '(Zhao,': [108, 307], 'X.,': [109, 308], 'Muller,': [110, 309], 'E.G.D.,': [111, 310], 'Rothstein,': [113, 312], 'R.': [114, 313, 666, 668, 702, 802, 952, 1215, 1251, 1294, 1560, 1602, 1726, 1813, 1862, 2368, 3351, 3470, 3652], '(1998)': [115, 314], 'Mol.': [116, 315, 768, 785, 809, 824, 953, 1295, 3352, 3471], 'Cell': [117, 316], '2,': [118, 317], '329–340).': [119, 318], 'Here,': [120, 319], 'we': [121, 320, 1037, 2237, 2416, 2429, 2639, 3093, 3206, 3215, 3242, 3277, 3676], 'use': [122, 321], 'highly': [123, 322, 1033], 'purified': [124, 323, 1034, 1272], 'recombinant': [125, 324, 1035, 1140, 1160, 1196, 1205, 2065, 2986], 'proteins': [126, 325, 1142, 1161, 1268, 1744, 3399, 3552], 'directly': [128, 327, 1039, 2947, 3339], 'demonstrate': [129, 328, 1040], 'that': [130, 329, 502, 972, 1049, 2339, 2549, 2565, 2730, 3149, 3191, 3233, 3367, 3517, 3612, 3752, 3771, 3788, 3805, 3814, 3913], 'Sml1': [132, 331, 1505, 1684, 1920, 2066, 2119, 2553, 2642, 2982, 3370, 3434, 3519, 3560, 3690, 3754, 3918], 'a': [135, 150, 154, 196, 334, 349, 353, 395, 409, 448, 452, 520, 595, 609, 695, 969, 990, 1013, 1053, 1130, 1274, 1395, 1498, 1696, 1776, 1783, 1839, 1949, 1966, 1987, 2102, 2109, 2193, 2244, 2321, 2359, 2435, 2550, 2597, 2602, 2630, 2649, 2655, 2674, 2691, 2700, 2750, 2767, 2773, 2777, 2804, 2848, 2863, 2908, 2935, 2993, 3002, 3016, 3029, 3067, 3113, 3117, 3132, 3145, 3228, 3255, 3262, 3373, 3381, 3545, 3570, 3578, 3686, 3785, 3810], 'strong': [136, 335], 'inhibitor': [137, 336, 1056, 3375], 'RNR.': [140, 339, 1059, 1138], 'Sml1p': [142, 161, 341, 360, 994, 1022, 1050, 1080, 1385, 1526, 1639, 1663, 1794, 1804, 2135, 2178, 2204, 2248, 2298, 2400, 2420, 2486, 2539, 2567, 2600, 2618, 2637, 2672, 3076, 3109, 3709, 3839], 'specifically': [143, 163, 342, 362, 1061, 1082, 3562, 3758], 'binds': [144, 164, 343, 363, 1062, 1083, 3563], 'Rnr1p': [148, 347, 1066, 1667, 1797, 1811, 1880, 2129, 2157, 2404, 2408, 2418, 2479, 2493, 2562, 2572, 2582, 2635, 2663, 2742, 2764, 2872, 2885, 2953, 2975, 3625], '1:1': [151, 350, 2127, 3579], 'ratio': [152, 351, 2125, 3580], 'with': [153, 352, 999, 1075, 1089, 1352, 1366, 1412, 1497, 1633, 1747, 1795, 1807, 1938, 2106, 2279, 2302, 2407, 2527, 2712, 2954, 2957, 3049, 3227, 3249, 3272, 3285, 3509, 3569, 3594, 3623, 3627, 4008], 'dissociation': [155, 354, 2611, 2838, 2909, 2928, 3147, 3571, 3638, 3787], 'constant': [156, 355, 1777, 1784, 2839, 2902, 2911, 2929, 3572, 3639], '0.4': [158, 357, 3574], 'μm.': [159, 358, 2309], 'Interestingly,': [160, 359, 1078], 'also': [162, 361, 757, 1081, 3178, 3757, 3998], 'mouse': [167, 180, 366, 379, 1086, 1100, 1159, 1197, 1206, 1669, 1718, 2337, 2987, 3089, 3101, 3123, 3175, 3197, 3246, 3280, 3323, 3632, 3727, 3762, 3773, 3826, 3842, 3860, 3885], 'R1': [170, 369, 478, 484, 645, 654, 1087, 1162, 1200, 1553, 1670, 2231, 2327, 2352, 3020, 3062, 3102, 3124, 3157, 3198, 3240, 3247, 3281, 3308, 3324, 3588, 3609, 3728, 3763, 3774, 3808, 3818, 3849, 3899, 3925], 'protein.': [171, 370, 1921, 3043, 3764, 3850], 'However,': [172, 371, 1092, 3299, 3765], 'inhibition': [174, 189, 373, 388, 1094, 1115, 2195, 2301, 3054, 3086, 3712, 3853, 3864, 3882], 'observed': [175, 374, 2585, 2793, 3074, 3171, 3216, 3837], 'an': [177, 376, 537, 1042, 1097, 1114, 1401, 1644, 2280, 2300, 2303, 2680, 2836, 2899, 3152, 3160, 3293, 3494], 'vitro': [179, 378, 1044, 1099, 2270, 3337], 'assay': [183, 382, 1048, 1103, 2018, 2273, 2995, 3828], 'less': [185, 384, 1105, 3056], 'pronounced': [186, 385, 1106, 3057], 'than': [187, 386, 1107, 3703], 'probably': [193, 392], 'occurs': [194, 393], 'via': [195, 394], 'different': [197, 396, 1117, 2467, 3411], 'mechanism.': [198, 397], 'resonance': [400, 1826], 'unit': [401, 1827], 'polyacrylamide': [402], 'gel': [403], 'electrophoresis': [404], '(RNR)1': [407], 'plays': [408], 'crucial': [410], 'role': [411], 'synthesis,': [414], 'catalyzing': [416], 'direct': [418, 3546], 'all': [421], 'four': [422], 'deoxyribonucleotides.': [425], 'Both': [426], 'mammalian': [430, 467, 910, 3391, 3420, 3488, 3541], 'reductases': [432, 2314], 'belong': [433], 'class': [438, 2317], 'Ia;': [439], 'class,': [442], 'active': [444, 490, 3880], 'enzymes': [445], 'consist': [446], 'large': [449], 'subunit': [450, 3665], 'small': [453], 'one': [454, 841, 1120, 2153, 2470, 2523, 2534, 2884], '(1Reichard': [455], 'P.': [456, 579, 706, 1225, 1227, 1570, 1572], 'Science.': [457], '1993;': [458], '260:': [459], '1773-1777Crossref': [460], 'PubMed': [461, 586, 677, 750, 774, 791, 815, 830, 864, 903, 963, 1188, 1241, 1258, 1305, 1586, 1609, 1737, 1820, 2031, 2051, 2375, 2394, 3362, 3460, 3481, 3659], 'Scopus': [462, 587, 678, 724, 751, 775, 792, 816, 831, 865, 904, 964, 1189, 1259, 1306, 1610, 1738, 1821, 2032, 2052, 2376, 3363, 3461, 3482, 3660], '(502)': [463], 'Google': [464, 589, 680, 726, 753, 777, 794, 818, 833, 867, 906, 966, 1191, 1242, 1261, 1308, 1587, 1612, 1740, 1823, 1878, 2034, 2054, 2378, 2395, 3365, 3463, 3484, 3662], 'Scholar).': [465, 558, 590, 631, 681, 727, 834, 868, 907, 1192, 1262, 1613, 1741, 1824, 1879, 2396, 3485, 3978], 'In': [466, 550, 623, 728, 2288, 2756, 2812, 3007, 3044, 3066, 3422, 3486, 3970], 'cells,': [468, 3542], 'these': [469, 2931], 'two': [470, 733, 758, 2519], 'non-identical': [471], 'homodimeric': [472, 2326], 'subunits': [473], 'encoded': [475], 'R2': [480, 592, 638, 647, 658, 688, 1164, 1207, 1592, 1719, 2329, 2365, 3042, 3064, 3079, 3176, 3286, 3301, 3500, 3843, 3894, 3907], 'genes,': [481], 'respectively.': [482, 2287], 'contains': [486], 'redox-active': [487], 'dithiols,': [488], 'site': [491, 539, 2347], 'binding': [492, 497, 512, 529, 651, 1023, 2184, 2228, 2340, 2362, 2401, 2616, 2737, 2850, 3098, 3325, 3605, 3775, 3781, 3815, 3846, 3896], 'diphosphate': [494], 'substrates': [495], 'sites': [498], 'triphosphates': [501], 'act': [503], 'as': [504, 1067, 1211, 1273, 1483, 1713, 1948, 1976, 2038, 2108, 2973, 3001, 3170, 3222, 3766, 3906], 'effectors.': [506], 'Substrate': [507], 'specificity': [508, 521, 2346], 'controlled': [510, 527], 'ATP,': [514], 'dATP,': [515], 'dTTP,': [516, 2476], 'or': [517, 533, 575, 882, 1525, 1589, 1668, 1914, 2056, 2215, 2265, 2798, 2862, 2868, 2958, 3026, 3611, 3813], 'dGTP': [518], 'site,': [522, 3881], 'whereas': [523], 'overall': [524], 'ATP': [531, 2107, 3000], '(active)': [532], 'dATP': [534, 3524], '(inactive)': [535], '(2Thelander': [540, 613, 3960], 'L.': [541, 614, 672, 806, 939, 1231, 1253, 1281, 1576, 1604, 1732, 1815, 1856, 2370, 2380, 3654, 3961], 'Gräslund': [542, 615, 1228, 1246, 1573, 1597, 1729, 3962], 'A.': [543, 547, 616, 620, 670, 800, 1229, 1247, 1278, 1574, 1598, 1730, 2924, 3963, 3967], 'Sigel': [544, 546, 617, 619, 3964, 3966], 'H.': [545, 618, 1870, 3965], 'Metal': [548, 621, 3968], 'Ions': [549, 622, 3969], 'Biological': [551, 624, 3971], 'Systems.': [552, 625, 3972], 'Marcel': [553, 626, 3973], 'Dekker,': [554, 627, 3974], 'New': [555, 628, 3975], 'York1994:': [556, 629, 3976], '109-129Google': [557, 630, 3977], 'Failure': [559], 'control': [561, 2757, 3412, 3490], 'size': [563], 'dNTP': [565, 888, 932, 975], 'pools': [566, 889, 976, 3443, 3555], 'and/or': [567], 'their': [568], 'relative': [569], 'amounts': [570, 2117, 2144], 'leads': [571], 'death': [574], 'genetic': [576], 'abnormalities': [577], '(3Reichard': [578], 'Annu.': [580], 'Rev.': [581], 'Biochem.': [582, 2026, 2047], '1988;': [583], '57:': [584], '349-374Crossref': [585], '(637)': [588], 'contributes': [594], 'tyrosyl-free': [596], 'radical,': [597], 'activity;': [603], 'radical': [605, 3954], 'generated': [607], 'binuclear': [610], 'iron': [611, 3956], 'center': [612], 'flexible': [633, 657], 'C-terminal': [634, 690, 2200, 2210, 2245, 3705, 3909], 'tail': [635, 660], 'polypeptide': [639, 3745], 'chain': [640, 1314, 1347], 'interaction,': [648, 2236, 2638], 'upon': [650], 'becomes': [661], 'rigid': [662], '(4Lycksell': [663], 'P.O.': [664], 'Ingemarson': [665, 1250, 1601, 1725], 'Davis': [667, 743, 766], 'Graslund': [669], 'Thelander': [671, 805, 1216, 1230, 1252, 1561, 1575, 1603, 1731, 1814, 2369, 3653], 'Biochemistry.': [673, 1254, 1605, 1733, 1816, 2371, 3655], '1994;': [674, 721, 1235, 1580], '33:': [675], '2838-2842Crossref': [676], '(44)': [679], 'Peptides': [682], 'peptidomimetics': [684, 3959], 'corresponding': [685, 1512, 1615, 2292], 'species': [696], 'specific': [697, 3307, 3496, 3940], 'way': [698, 3905], '(5Liuzzi': [699], 'M.': [700, 714, 821, 1217, 1562, 2384], 'Deziel': [701], 'Moss': [703], 'N.': [704], 'Beaulieu': [705, 1224, 1569], 'Bonneau': [707], 'A.M.': [708], 'Bousquet': [709], 'C.': [710], 'Chafouleas': [711], 'J.G.': [712], 'Garneau': [713], 'Jaramillo': [715], 'J.': [716, 1232, 1577, 2027, 2385], 'Krogsrud': [717], 'R.L.': [718], 'et': [719, 1873], 'al.Nature.': [720], '15:': [722], '695-698Crossref': [723], '(126)': [725], 'Saccharomyces': [729], 'cerevisiae,': [730], 'there': [731, 755, 3543], 'encoding': [735, 760], 'R1-like': [736], 'proteins,': [737, 762, 1036, 1201, 3241], 'RNR1': [738], 'RNR3': [740], '(6Elledge': [741], 'S.J.': [742, 765, 823, 897, 3454], 'R.W.': [744, 767], 'Genes': [745, 898, 3455], 'Dev.': [746, 899, 3456], '1990;': [747, 1185], '4:': [748], '740-751Crossref': [749], '(249)': [752], 'Scholar);': [754, 3663], 'R2-like': [761], 'RNR2': [763], '(7Elledge': [764], 'Cell.': [769, 786, 810, 825, 855, 954, 1296, 3353, 3472], 'Biol.': [770, 787, 811, 826, 1233, 1578, 2386], '1987;': [771, 788], '7:': [772, 789], '2783-2793Crossref': [773], '(165)': [776], 'Scholar,': [778, 819, 1243, 3464], '8Hurd': [779], 'H.K.': [780], 'Roberts': [781, 783], 'C.W.': [782], 'J.W.': [784, 1182], '3673-3677Crossref': [790], '(54)': [793], 'Scholar)': [795, 967, 1309, 1588, 3366], 'andRNR4': [796], '(9Wang': [797], 'P.J.': [798], 'Chabes': [799, 937], 'Casagrande': [801], 'Tian': [803], 'X.C.': [804], 'Huffaker': [807], 'T.C.': [808], '1997;': [812, 827], '17:': [813, 828], '6114-6121Crossref': [814], '(95)': [817], '10Huang': [820], 'Elledge': [822, 896, 3453], '6105-6113Crossref': [829], '(159)': [832], 'targets': [844], 'Mec1-Rad53-dependent': [847], 'damage/cell': [849], 'checkpoint': [851], 'pathway': [852], '(11Weinert': [853], 'T.': [854], '1998;': [856, 900, 955, 1297, 3354, 3457, 3473], '94:': [857], '555-558Abstract': [858], 'Full': [859, 861, 958, 960, 1238, 1300, 1302, 1583, 2391, 3357, 3359, 3476, 3478], 'Text': [860, 862, 959, 961, 1239, 1301, 1303, 1584, 2392, 3358, 3360, 3477, 3479], 'PDF': [863, 962, 1240, 1304, 1585, 2393, 3361, 3480], '(237)': [866], 'Overexpression': [869], 'suppresses': [875], 'lethality': [877, 988], 'strains': [879], 'lacking': [880], 'Mec1': [881], 'Rad53,': [883], 'supposedly': [884], 'increasing': [886, 1808, 2116, 2660, 2745, 3038], '(12Desany': [890, 3447], 'B.A.': [891, 3448], 'Alcasabas': [892, 3449], 'A.A.': [893, 3450], 'Bachant': [894, 3451], 'J.B.': [895, 3452], '12:': [901, 3458], '2956-2970Crossref': [902, 3459], '(384)': [905, 3462], 'Unlike': [908], 'enzyme,': [911], 'inhibited': [917, 2267, 3532], 'physiological': [919, 3374, 3534], 'concentrations': [920, 1533, 1809, 2661, 2740, 3039, 3535], 'dATP;': [922], 'observation': [924], 'explains': [925], 'positive': [927, 2110, 3003], 'correlation': [928, 3547], 'between': [929, 1662, 2128, 2177, 3075, 3099, 3548, 3838], 'enzyme': [930], 'levels': [933, 3549], '2A.': [936], 'Thelander,': [940, 1282], 'submitted': [941], 'publication.': [943], 'Zhaoet': [945, 3344], 'al.': [946, 3345], '(13Zhao': [947, 1289, 3346], 'X.': [948, 1290, 3347, 3466], 'Muller': [949, 1291, 3348, 3467], 'E.G.': [950, 1292, 3349, 3468], 'Rothstein': [951, 1293, 3350, 3469, 3987], '2:': [956, 1298, 3355, 3474], '329-340Abstract': [957, 1299, 3356, 3475], '(603)': [965, 1307, 3364, 3483], 'identified': [968], 'negatively': [973], 'affects': [974], 'yeast,': [978, 3423], 'they': [980], 'called': [981], 'it': [982, 1358, 1701, 2576, 2728, 3748], 'Sml1p.': [983, 1077, 2892, 3105, 3298], 'Deletion': [984], 'ofSML1': [985], 'rescued': [986], 'mec1': [991], 'orrad53': [992], 'strain.': [993], 'interact': [998, 1806], 'using': [1004, 1072, 1154, 1171, 1320, 1544, 1643, 1677, 2101], 'two-hybrid': [1006], 'system': [1007, 3868, 3886], 'co-immunoprecipitations.': [1009], 'For': [1010, 2310, 3631], 'reason,': [1012], 'suggested,': [1020], 'where': [1021, 2477, 2759], 'would': [1028, 3756], 'enzyme.': [1031], 'Using': [1032, 2828, 2893], 'now': [1038], 'indeed': [1052], 'very': [1054, 3133, 3870], 'potent': [1055], 'It': [1060, 2331], 'biosensor': [1070, 1675, 3832], 'technique': [1071], 'sensor': [1073, 1711, 2650, 2667, 2768, 3114, 3162], 'chips': [1074], 'immobilized': [1076, 1704, 1803, 2640, 2771, 2880, 2891, 3111, 3204], 'high': [1090], 'affinity.': [1091], 'seen': [1095, 2060, 2459], 'system,': [1111], 'indicates': [1113, 2564], 'mechanism': [1116, 3854, 3930], 'from': [1118, 1316, 1530, 1624, 1631, 1655, 2259, 2468, 2500, 2917, 2930, 3332], 'These': [1123, 2856, 3600], 'findings': [1124], 'may': [1125, 3409, 3521, 3602, 3798, 3855, 3873, 3891], 'be': [1126, 2358, 2458, 2505, 2710, 3401, 3647, 3936], 'used': [1127], 'develop': [1129], 'new': [1131, 3382, 3946], 'generation': [1132], 'antiproliferative': [1134, 3941], 'drugs': [1135], 'targeted': [1136], 'Rnr1,': [1143], 'Rnr2,': [1144], 'Rnr4': [1146, 1267, 2216], 'were': [1147, 1165, 1269, 1420, 1460, 1528, 1534, 1745, 1773, 1932, 1960, 2008, 2014, 2148, 2854, 2915, 2971, 2990, 3318], 'expressed': [1148, 1166, 1387], 'Escherichia': [1150], 'coli': [1151, 1390, 3635], 'BL21(DE3)': [1152], 'bacteria': [1153, 1170, 1392, 1419], 'pET3a': [1156, 1363], 'expression': [1157], 'vector;': [1158], 'E.': [1168, 1249, 1389, 1600, 3634], 'coliBL21(DE3)pLysS': [1169], 'same': [1173, 1368, 1481, 1974, 2277, 2509, 2666, 2761, 2785, 2969, 3166, 3218, 3904], 'vector': [1174, 1364], '(14Studier': [1175], 'F.W.': [1176], 'Rosenberg': [1177], 'A.H.': [1178], 'Dunn': [1179], 'J.J.': [1180], 'Dubendorff': [1181], 'Methods': [1183], 'Enzymol.': [1184], '185:': [1186], '60-89Crossref': [1187], '(6006)': [1190], 'Purification': [1193], 'made': [1210, 3319], 'described': [1212, 1714], 'earlier': [1213, 1546], '(15Davis': [1214], 'Mann': [1218, 1563], 'G.J.': [1219, 1245, 1564, 1596], 'Behravan': [1220, 1565, 1727], 'G.': [1221, 1566, 1728], 'Soucy': [1222, 1567], 'F.': [1223, 1568], 'Lavallee': [1226, 1571], 'Chem.': [1234, 1579, 2387], '269:': [1236, 1581], '23171-23176Abstract': [1237, 1582], '16Mann': [1244, 1595], 'Ochiai': [1248, 1599], '1991;': [1255, 1606, 1875], '30:': [1256, 1607], '1939-1947Crossref': [1257, 1608], '(144)': [1260, 1611], 'Rnr2': [1265, 2214], 'coexpressed': [1270], 'heterodimer.': [1275, 3630, 3720], '3V.': [1276], 'Domkin,': [1277], 'Chabes,': [1279], 'manuscript': [1283, 3992], 'preparation.': [1285], 'TheSML1': [1286], 'coding': [1287], 'sequence': [1288, 1373, 2201, 3734, 3738], 'amplified': [1311], 'polymerase': [1313, 1346], 'reaction': [1315, 1348], 'genomic': [1318], 'following': [1322, 2588], 'oligonucleotides:': [1323], '5′-CAA': [1324], 'TAA': [1325], 'TTT': [1326], 'CCC': [1327], 'CAT': [1328, 1340], 'ATG': [1329], 'CAA': [1330], 'AAT': [1331], 'TCC-3′': [1332], '5′-AAA': [1334], 'GGA': [1335], 'TCC': [1336], 'TTA': [1337], 'GAA': [1338], 'GTC': [1339], 'TTC': [1341], 'CTC': [1342], 'GAC-3′.': [1343], 'After': [1344, 1415, 1441, 1922, 3084], 'product': [1349], 'cleaved': [1351], 'NdeI': [1353], 'BamHI': [1355], 'restriction': [1356, 1369], 'endonucleases,': [1357], 'cloned': [1360], 'into': [1361, 2577, 3505], 'digested': [1365], 'enzymes.': [1370, 3862], 'SML1': [1372], 'resulting': [1376], 'plasmid': [1377], 'checked': [1379], 'sequencing.': [1382], 'Recombinant': [1383, 2093], 'BL21(DE3)pLysS': [1391], 'growing': [1394], 'culture': [1396], 'at': [1397, 1406, 1447, 1470, 1540, 1695, 1775, 1927, 1983, 1992, 2152, 2541, 2601, 2673, 2803, 2818, 3581], '37': [1398], '°C': [1399, 1452, 1472, 1781], 'optical': [1402], 'density': [1403], '0.6': [1405], '600': [1407], 'nm,': [1408, 1542], 'followed': [1409, 3143, 3260, 3783], 'induction': [1411], '0.5': [1413], 'mmisopropyl-1-thio-β-d-galactopyranoside.': [1414], '3': [1416], 'h,': [1417], 'harvested': [1421], 'centrifugation,': [1423], 'resuspended': [1424], '50': [1426], 'mm': [1427, 1432, 1690, 1751, 1756, 1760, 1763, 1890, 1895, 1899, 1903, 1907], 'Tris-HCl,': [1428], 'pH': [1429, 1693, 1753, 1892], '7.4,': [1430, 1754, 1893], '1': [1431, 1454, 1759, 1835, 1843, 2085], 'EDTA,': [1433, 1761], 'lysed': [1435], 'freezing': [1437], 'liquid': [1439], 'nitrogen.': [1440], 'thawing,': [1442], 'lysate': [1444], 'centrifuged': [1446, 1489, 1979], '150,000': [1448], '×': [1449], 'g,': [1450], '4': [1451, 1993], 'h.': [1455], 'Proteins': [1456], 'supernatant': [1459], 'precipitated': [1461], 'adding': [1463], 'solid': [1464], 'ammonium': [1465, 2076], 'sulfate': [1466, 2077], '25%': [1468], 'saturation': [1469], '0': [1471], '(0.136': [1473], 'g/ml).': [1474], 'pellet': [1476], 'dissolved': [1478], 'buffer': [1482, 1749, 1975], 'above;': [1484], 'finally,': [1485], 'solution': [1487, 3118, 3158], 'through': [1490, 2596], 'Ultrafree-MC': [1491], 'Millipore': [1492], '30,000': [1493], 'NMWL': [1494], 'filter': [1495], 'units': [1496], '30-kDa': [1499], 'cut-off': [1500], 'value': [1501, 1616, 2752, 2787, 3169], 'obtain': [1503, 2629], 'pure': [1504, 2064, 2088, 2985], 'filtrate.': [1509], 'N-acetylated': [1510], 'peptides': [1511, 2211, 2220], 'last': [1515], '9': [1516], 'amino': [1517, 1626, 1657, 3732], 'acids': [1518], 'either': [1520, 1665], 'Rnr2p': [1521, 2264], '(GAFTFNEDF),': [1522], 'Rnr4p': [1523, 2266], '(KEINFDDDF),': [1524], '(QGKVEEMDF)': [1527], 'ordered': [1529], 'Genosys.': [1531], 'Protein': [1532], 'determined': [1535, 1642, 1799, 2039], 'reading': [1537, 3990], 'absorbance': [1539], '280': [1541], 'published': [1547], 'E1%1': [1548], 'cm': [1549, 1647], '12': [1551], '(mouse': [1552, 1591], 'proteins;': [1557], 'Ref.': [1558, 1594], '15Davis': [1559], '13.7': [1590], 'protein;': [1593, 3214], 'Rnr2p/Rnr4p': [1619, 2146, 2181, 2799, 2955], 'heterodimer': [1620, 2147, 2182, 2800, 3597], '(12.9)': [1621], 'obtained': [1623, 2069, 2916, 2972, 3271], 'quantitative': [1625, 2631], 'acid': [1627, 1658, 3733], 'hydrolysis': [1628], 'aliquots': [1630], 'solutions': [1632, 1640, 2658, 2765], 'known': [1634, 2222], 'absorbance.': [1635], 'concentration': [1637, 1697, 1841, 2037, 2603, 2675, 2805, 2824, 3716], 'E': [1645], '1%1': [1646], '7.1': [1649], 'based': [1650], 'on': [1651, 1705, 1934, 2249, 2434, 2984, 3060, 3112, 3607, 3714], 'calculations': [1652], '(DNAstar,': [1653], 'Inc.)': [1654], 'composition.': [1659], 'analysis': [1676, 2511], 'BIAcore': [1679], 'method': [1680, 2043], '(Biacore': [1681, 2897], 'AB).': [1682], 'prepared': [1687], '10': [1689, 1898], 'sodium': [1691], 'acetate,': [1692, 1758, 1765, 1897, 1901], '5.2,': [1694], '0.44': [1699, 2677], 'mg/ml;': [1700], 'then': [1703, 2430, 2653], 'dextran': [1707, 1851, 2646], 'layer': [1708, 1852, 2647], 'chip': [1712, 2651, 2769, 3163], 'previously': [1715, 2333], '(17Rova': [1721], 'U.': [1722, 1854], 'Goodtzova': [1723], 'K.': [1724, 1864], '1995;': [1734], '34:': [1735], '4267-4275Crossref': [1736], '(108)': [1739], 'ligand': [1743], 'equilibrated': [1746], 'running': [1748], '(10': [1750], 'Hepes-KOH,': [1752, 1891], '200': [1755, 3694], 'potassium': [1757, 1896], '5': [1762, 1787, 1902, 2754, 2814, 2923, 3225], 'magnesium': [1764, 1900], '0.05%': [1767], 'Surfactant': [1768], 'P20': [1769], '(BIAcore).': [1770], 'interactions': [1772], 'temperature': [1778], '22': [1780], 'flow': [1785], 'μl/min.': [1788], 'Kinetics': [1789], 'allowing': [1801], '(18Ingemarson': [1812, 2367, 3651], '1996;': [1817, 2372, 3656], '35:': [1818, 2373, 3657], '8603-8609Crossref': [1819, 2374, 3658], '(41)': [1822, 2377, 3661], '(RU)': [1828], 'proportional': [1830], 'mass,': [1833], 'RU': [1836, 2697, 2791, 2853], 'corresponds': [1837, 2698], 'surface': [1840, 2692], 'pg': [1844, 2704, 2867], 'protein/mm2': [1846], '100-nm': [1849], 'thick': [1850], '(19Jönsson': [1853], 'Fägerstam': [1855], 'Ivarsson': [1857], 'B.': [1858, 1860, 1868, 2023], 'Johnsson': [1859], 'Karlsson': [1861], 'Lundh': [1863], 'Lofas': [1865], 'S.': [1866, 2382], 'Persson': [1867], 'Roos': [1869], 'Ronnberg': [1871], 'I.': [1872], 'al.BioTechniques.': [1874], '11:': [1876], '620-627PubMed': [1877], '(500': [1881], 'μg)': [1882, 2151, 2159], 'incubated': [1884], '0.1': [1886, 1906, 2006, 2807, 2845, 3120, 3208, 3649], 'ml': [1887, 1964, 2007], '20': [1889], '100': [1894], 'dithiothreitol,': [1904], 'dTTP': [1908, 2443, 2960, 3250, 3284, 3804], 'if': [1909, 2399], 'indicated,': [1910], 'presence': [1913, 2114, 2423, 2998, 3014, 3025], 'absence': [1915, 2474, 3027], '90': [1917], 'μg': [1918, 1940, 2165], '30': [1923, 2285], 'min': [1924], 'incubation': [1926, 2592], '25': [1928], '°C,': [1929], 'samples': [1931, 1959], 'cooled': [1933], 'ice': [1935], 'mixed': [1937, 3244, 3278], '0.75': [1939], 'bovine': [1942, 2795], 'liver': [1943], 'catalase': [1944, 1946, 2020], '(Sigma);': [1945], 'served': [1947], 'marker': [1950], 'determination': [1953], 'sedimentation': [1955], 'coefficients.': [1956], '0.1-ml': [1958], 'layered': [1961], 'onto': [1962], '4.0': [1963], '5–20%': [1967], 'linear': [1968], 'gradient': [1969, 2437, 2598], 'sucrose': [1971, 2436], 'above,': [1977], '14': [1981], 'h': [1982], '40,000': [1984], 'rpm': [1985], 'Beckman': [1988], 'SW': [1989], '60': [1990, 2782, 2790], 'rotor': [1991], '°C.': [1994], 'bottom': [1996], 'each': [1998, 2012], 'tube': [1999], 'punctured,': [2001], 'fractions': [2003, 2514, 2559], 'approximately': [2005], 'collected.': [2009], 'Aliquots': [2010], 'fraction': [2013], 'removed': [2015], 'spectrophotometric': [2017], '(20Chance': [2022], 'Herbert': [2024], 'D.': [2025], '1950;': [2028], '46:': [2029], '402-414Crossref': [2030], '(91)': [2033], 'Scholar),': [2035, 2055], 'Bradford': [2042], '(21Bradford': [2044], 'M.M.': [2045], 'Anal.': [2046], '1976;': [2048], '72:': [2049], '248-254Crossref': [2050], '(217544)': [2053], 'SDS-PAGE': [2057, 2516], 'analysis.': [2058], 'As': [2059, 2253, 3106], 'Fig.': [2062, 2256, 2813, 2922, 3224], '1,': [2063], 'after': [2070, 3444], 'only': [2071, 2306, 2780, 3181], 'three': [2072], 'purification': [2073], 'steps:': [2074], 'ultracentrifugation,': [2075], 'fractionation,': [2078], 'ultrafiltration.': [2080], 'yield': [2082], 'about': [2084, 2140, 2275, 2307, 2781, 2789, 2876, 2904, 2939, 3693], 'mg': [2086], 'protein/liter': [2089], 'bacterial': [2091], 'culture.': [2092], 'assayed': [2098], 'CDP': [2103, 2994], 'assay,': [2105], 'effector': [2111, 2427, 3004], '(Fig.': [2121, 2438, 2482, 2753, 3005, 3128], '2).': [2122], 'molar': [2124, 3031, 3687], 'monomer': [2130, 2455, 2873, 2886], '(M': [2131, 2136], 'r': [2132, 2137], '100,000)': [2133], '11,800)': [2138], 'gave': [2139, 2679, 2776, 2934, 3164], '50%': [2141], 'inhibition.': [2142, 3932], 'varied': [2149], '(1–32': [2150], 'fixed': [2154, 3017], 'amount': [2155, 2716, 3018], '(2': [2158], '0,': [2161], '0.2,': [2162], '2': [2164], 'Sml1p;': [2167], 'experiment': [2169], 'did': [2170, 2487, 2619, 2963, 3584], 'give': [2172], 'any': [2173], 'evidence': [2174], 'competition': [2176, 3072, 3593, 3835], 'Rnr1p.': [2186, 2627, 2827, 3275], 'Instead': [2187], 'data': [2189, 3338, 3405], 'fitted': [2190], 'noncompetitive': [2194], '(data': [2196, 2612, 2809, 3081, 3184], 'shown).': [2198, 2614, 2811, 3083, 3186], 'shows': [2205, 3772], 'some': [2206], 'homology': [2207], 'proteins.': [2217, 2330], 'Because': [2218], 'such': [2219, 3945], 'preventing': [2234], 'R1/R2': [2235], 'wanted': [2238, 3094], 'study': [2240], 'influence': [2242, 2489, 2621, 3586], 'nonapeptide': [2246, 2291], 'activity.': [2252], '3,': [2257], 'nonapeptides': [2258], 'C': [2261, 2295], 'terminus': [2262, 2296], 'extent': [2278], 'IC50': [2281, 2304], '44': [2283], 'μm,': [2286, 2941], 'contrast,': [2289, 3487], 'showed': [2299], '300': [2308], 'enzymatic': [2311], 'activity,': [2312, 2415], 'Ia-type': [2318], 'must': [2319], 'form': [2320], 'heterodimeric': [2322], 'complex': [2323, 3295, 3643], 'composed': [2324], 'demonstrated': [2334, 2518], 'effectors': [2342, 3235], 'substrate': [2345], 'promotes': [2348], 'formation': [2349, 2410, 2445], 'dimer,': [2353], 'believed': [2356], 'prerequisite': [2360], 'dimer': [2366, 2409, 2529, 2573, 3819], 'Scholar,23Thelander': [2379], 'Eriksson': [2381], 'Åkerman': [2383], '1980;': [2388], '255:': [2389], '7426-7432Abstract': [2390], 'To': [2397, 2628, 3187], 'test': [2398], 'might': [2405], 'interfere': [2406], 'thereby': [2412], 'preincubated': [2417], 'dTTP;': [2428], 'analyzed': [2431], 'mixture': [2433, 2593], '4).': [2439, 2483], 'addition': [2441, 3303, 3793], 'induced': [2444, 3802], 'dimers': [2447, 2501, 2563], 'tetramers': [2449], 'Rnr1p,': [2452, 2532], 'no': [2454, 2498, 3390, 3397, 3592], 'peak': [2456, 2525, 2530, 2536], 'could': [2457, 2504, 3400, 3948], 'gradient.': [2462, 2546], 'This': [2463, 2720, 3253, 3407, 3796, 3933], 'pattern': [2464], 'quite': [2466, 3750], 'monomers': [2480, 2503, 2583], 'dominate': [2481], 'Addition': [2484], 'distribution': [2491], 'gradient,': [2496], 'shift': [2499, 2580], 'observed.': [2506, 3599], 'At': [2507], 'time,': [2510, 3254], 'clearly': [2517], 'peaks': [2520], 'Sml1p,': [2522, 2707, 2772, 2881, 3034, 3092, 3205, 3777], 'minor': [2524], 'cosedimenting': [2526], 'major': [2535], 'representing': [2537], 'free': [2538], 'sedimenting': [2540], 'top': [2543], 'fact': [2548], 'portion': [2551], 'present': [2556], 'containing': [2560, 2599, 2659, 3119], 'can': [2568, 2709], 'without': [2574, 2770, 2959], 'dissociating': [2575], 'monomers.': [2578], 'No': [2579, 3316], 'toward': [2581], 'under': [2586], 'conditions:': [2589], 'Rnr1p/Sml1p/dTTP': [2591], 'sedimented': [2595], '0.15': [2605], 'mg/ml': [2606, 2678, 2808, 3121, 3209], 'throughout': [2607], 'minimize': [2609], 'Rnr1p-Sml1p': [2610], 'Therefore,': [2615, 3538, 3851], 'monomer/dimer': [2623, 3589], 'equilibrium': [2624, 2746, 2819, 2837, 2927, 3153, 3230, 3264], 'description': [2632], 'injected': [2654, 2715, 2802, 2826, 2977, 3127, 3207], 'series': [2656, 2762], 'chip.': [2668], 'immobilization': [2670], 'increase': [2681], '91': [2683, 2688, 2696], 'RU,': [2684], 'correspond': [2686, 2858], 'pg/mm2.': [2689], 'With': [2690], '0.8': [2694], 'mm2,': [2695], 'total': [2701, 2714, 2864], '72.8': [2703], 'bound': [2706, 2874, 2887, 3296, 3922], 'compared': [2711, 3622], '15.4': [2718], 'μg.': [2719], 'low': [2721, 3167], 'degree': [2722], 'attachment': [2724], '(only': [2725], '0.0005%)': [2726], 'makes': [2727, 3806], 'unlikely': [2729], 'attached': [2734], 'multiple': [2736], 'sites.': [2738], 'Increasing': [2739], 'resulted': [2743, 3179, 3311], 'values,': [2747], 'approached': [2749], 'maximal': [2751, 2849], 'A).': [2755], 'experiments,': [2758, 3833], 'passed': [2766], '"bulk': [2774], 'effect"': [2775], 'RU.': [2783], 'background': [2786, 3168, 3182, 3314], 'serum': [2796], 'albumin': [2797], 'B,': [2815], 'plotted': [2821, 3036], 'against': [2822, 3037], 'GraphPad': [2830], 'Prism': [2831], 'program': [2832], '(GraphPad': [2833], 'Software,': [2834], 'Inc.),': [2835], '(K': [2840], 'D)': [2841], '0.41': [2843], '±': [2844], 'μm': [2846, 3575, 3650], '691': [2852, 2860], 'obtained.': [2855], 'values': [2857, 3183], 'pg/mm2': [2861], '553': [2866], '5.5': [2869], 'fmol': [2870, 2878], '6.2': [2877], 'i.e.': [2882], 'nearly': [2883], 'per': [2888], 'molecule': [2889], 'BIAevaluation': [2895], 'software': [2896], 'AB),': [2898], 'association': [2900, 3135, 3257], 'rate': [2901, 2910, 2932], '153,000m−1': [2905], 's−1': [2906, 2914], '0.04': [2913], 'lower': [2919], 'curves': [2920], 'Calculating': [2925], 'constants': [2933], 'K': [2936], 'D': [2937, 2949], '0.25': [2940], 'close': [2944], 'determinedK': [2948], '.': [2950], 'Mixing': [2951], 'heterodimer,': [2956], 'before': [2961, 3251, 3288], 'injection,': [2962, 3131, 3289], 'affect': [2965, 3236], 'curves;': [2967], 'results': [2970], 'alone.': [2978], 'effects': [2980], 'tested': [2991], '6).': [3006], 'figure,': [3009], '300-fold': [3030], 'excess': [3032], 'contrast': [3045], 'situation': [3048, 3408], 'RNR,': [3052], 'dependent': [3059], 'ratio.': [3065], 'double': [3068], 'reciprocal': [3069], 'plot,': [3070], 'clear': [3071, 3229, 3263, 3834], 'observing': [3085], 'characterize': [3096], 'before,': [3107], 'chip,': [3115], '7).': [3129], 'On': [3130, 3685], 'rapid': [3134, 3256, 3780], 'phase': [3136, 3140, 3148, 3258], 'observed;': [3138], 'immediately': [3142], 'prolonged': [3146, 3786], 'never': [3150], 'reached': [3151], 'plateau.': [3154, 3231], 'Injecting': [3155], 'over': [3159], 'empty': [3161], 'earlier.': [3172], 'Injection': [3173], 'exclude': [3188], 'possibility': [3190], 'unexpected': [3193, 3751], 'behavior': [3194], 'due': [3201], 'improperly': [3203], 'type': [3219], 'A,': [3226], 'Knowing': [3232], 'conformation': [3238], 'next': [3243], 'injection.': [3252], 'plateau;': [3265], 'sensorgram': [3267, 3270, 3770], 'resembled': [3268], 'Finally,': [3276], 'plus': [3283], 'expecting': [3290], 'see': [3292], 'R1-R2': [3294, 3642], 'almost': [3304, 3313], 'abolished': [3305], 'binding,': [3309], 'values.': [3315], 'attempts': [3317], 'quantify': [3321, 3683], 'data,': [3326], 'because': [3327], 'sensorgrams': [3329], 'deviated': [3330], 'widely': [3331], 'standard': [3333], 'curves.': [3334], 'Our': [3335], 'prove': [3340], 'hypothesis': [3342], 'concept': [3383], 'field.': [3387], 'So': [3388], 'far,': [3389], 'homologue': [3392], 'identified,': [3395], 'homologous': [3398], 'found': [3402], 'available': [3404], 'bases.': [3406], 'reflect': [3410, 3892], 'cells.': [3421, 3558], 'activation': [3425], 'suppression': [3431], 'lead': [3436], 'increased': [3438], 'damage': [3446], '13Zhao': [3465], 'cells': [3489, 3503], 'S-phase/DNA-damage': [3495], 'stabilization': [3497], 'until': [3502], 'enter': [3504], 'mitosis,': [3506], 'combination': [3508], 'negative': [3510], 'feedback': [3511, 3525], 'dATP2.': [3514], 'We': [3515, 3911, 3979, 3997], 'suggest': [3516], 'substitute': [3522], 'regulation,': [3526], 'since': [3527], 'dATP.': [3537], 'unlike': [3539, 3708], 'saturation.': [3582], 'Binding': [3583], 'equilibrium,': [3590], 'R2-R4': [3596, 3719], 'observations': [3601], 'indicate': [3603, 3799], 'separate': [3604], 'areas': [3606], 'affinity': [3614], 'Rnr1p/Sml1p': [3617], 'much': [3620, 3672], 'higher': [3621], 'R2/R4': [3629], 'RNRs,': [3636], 'reported': [3645], 'around': [3648], 'appears': [3671], 'weaker,': [3673], 'however,': [3674], 'have': [3677], 'yet': [3679], 'able': [3681], 'it.': [3684], 'basis,': [3688], 'times': [3695], 'more': [3696], 'efficient': [3697, 3871], 'inhibiting': [3699], 'its': [3704], 'nonapeptide.': [3706], 'Furthermore,': [3707, 3822], 'inhibition,': [3710], 'peptide': [3711], 'depends': [3713], 'show': [3730], '68%': [3731], 'identity,': [3735], 'similarities': [3739], 'distributed': [3741], 'along': [3742], 'whole': [3744], 'chain.': [3746], 'Still,': [3747], 'indicated': [3767], 'initial': [3779], 'prevented': [3790], 'dTTP.': [3795], 'finding': [3797, 3934], 'conformational': [3800], 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