Title: Effects of ELF electric field on proliferation of mouse osteoblastic cells
Abstract: MTT method and flow cytometry were used to detect the proliferation of osteoblastic cells after the cells were exposed to the low frequency, low amplitude electric field. The results showed that the cells exposed to the electric field of 10 Hz, 200 mV cm−1 for 20 min grew 59% more rapidly than the control cells, and also 52.94% more cells were in the S-phase. A fluorescence technique was used to measure the membrane fluidity and the change of the Ca2+ concentration inside the cells ([Ca2+]i), in order to study mechanism of the influence on cell proliferation after the cells were exposed to electric field. Our results show that the R value increased 27.38% compared to the control after the cells were treated with electric field of 10 Hz, 200 mV cm−1. The cell membrane viscosity increased 19.57% compared to the control after the treatment.
Publication Year: 1998
Publication Date: 1998-12-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 8
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