Title: Comparison of ninhydrin, fluorescamine, and o-phthaldialdehyde for the detection of amino acids and peptides and their effects on the recovery and composition of peptides from thin-layer fingerprints
Abstract: Amino acids and peptides separated by chromatography and electrophoresis on thin layers of cellulose or silica gel were located by spraying them with either ninhydrin, fluorescamine, or o-phthaldialdehyde. o-Phthaldialdehyde and ninhydrin permitted the detection of amino acids in the 50–200-pmol range and were superior to fluorescamine. Ninhydrin and fluorescamine, on the other hand, were better for the detection of peptides. Proline and peptides with amino-terminal proline were best detected as black spots when sprayed with fluorescamine, whereas peptides with amino-terminal proline which also contained ϵ-amino groups were more easily visualized with ninhydrin or o-phthaldialdehyde. A method is described for the elution of peptides from thin layers with 6 n HCl. Most small peptides were recovered in yields of at least 80%, ninhydrin-reacted peptides in somewhat lower yields. Only fluorescamine-treated peptides could be recovered from thin layers without destruction of any amino acid.
Publication Year: 1977
Publication Date: 1977-05-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 52
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