Title: The initiation cascade for chromosome replication in wild-type and Dam methyltransferase deficient Escherichia coli cells.
Abstract: Research Article15 April 1994free access The initiation cascade for chromosome replication in wild-type and Dam methyltransferase deficient Escherichia coli cells. A. Løbner-Olesen A. Løbner-Olesen University of Colorado at Boulder 80309-0347. Search for more papers by this author F.G. Hansen F.G. Hansen University of Colorado at Boulder 80309-0347. Search for more papers by this author K.V. Rasmussen K.V. Rasmussen University of Colorado at Boulder 80309-0347. Search for more papers by this author B. Martin B. Martin University of Colorado at Boulder 80309-0347. Search for more papers by this author P.L. Kuempel P.L. Kuempel University of Colorado at Boulder 80309-0347. Search for more papers by this author A. Løbner-Olesen A. Løbner-Olesen University of Colorado at Boulder 80309-0347. Search for more papers by this author F.G. Hansen F.G. Hansen University of Colorado at Boulder 80309-0347. Search for more papers by this author K.V. Rasmussen K.V. Rasmussen University of Colorado at Boulder 80309-0347. Search for more papers by this author B. Martin B. Martin University of Colorado at Boulder 80309-0347. Search for more papers by this author P.L. Kuempel P.L. Kuempel University of Colorado at Boulder 80309-0347. Search for more papers by this author Author Information A. Løbner-Olesen1, F.G. Hansen1, K.V. Rasmussen1, B. Martin1 and P.L. Kuempel1 1University of Colorado at Boulder 80309-0347. The EMBO Journal (1994)13:1856-1862https://doi.org/10.1002/j.1460-2075.1994.tb06454.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info 'Newborn' Escherichia coli B/r cells, obtained by membrane elution, were used to study the cell cycles of wild-type and Dam methyltransferase mutants. In wild-type cells, initiation of chromosome replication was synchronous and tightly controlled. In dam mutants, initiation was altered, but not random. We propose that this is due to the absence of an initiation cascade caused by liberated DnaA molecules, and that this cascade normally synchronizes initiation. The dam- cells contained mainly two, three or four replication origins, and this affected nucleoid partitioning as well as cell division. In cultures growing with a 50 min doubling time, a variety of cell cycles were present and half the origins were used every 25 min. Some cells had a 25 min interdivision time, whereas others had an interdivision time longer than the generation time. Partitioning of nucleoids containing unequal numbers of replication origins could also be readily observed by fluorescence microscopy in the dam mutant. Based upon these observations we propose that the dam mutant is also an initiation cascade mutant. Previous ArticleNext Article Volume 13Issue 81 April 1994In this issue RelatedDetailsLoading ...