Publication Information

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2323, 2339, 2351, 2356, 2402, 2416, 2435, 2471, 2489, 2505, 2512, 2526, 2531, 2607, 2612, 2679, 2729, 2741, 2876, 2879, 2934, 2940, 2959, 2962, 2978, 2984, 3003, 3007, 3016, 3044, 3047, 3057, 3084, 3115, 3164, 3170, 3191, 3196, 3218, 3223, 3230, 3242, 3267, 3282, 3303, 3319, 3324, 3336, 3355, 3360, 3367, 3413, 3425, 3436, 3445, 3449, 3476, 3523, 3545, 3550, 3554, 3565, 3576, 3579, 3588, 3593, 3601, 3624, 3628, 3631, 3646, 3653, 3685, 3688, 3692, 3699, 3721, 3739, 3763, 3809, 3820, 3832, 3839, 3906, 3925, 4041, 4060, 4080, 4087, 4090, 4100, 4162, 4183, 4199, 4217, 4226, 4289, 4304, 4315, 4354, 4375, 4395, 4451, 4469, 4527, 4530, 4536, 4596, 4615, 4643, 4650, 4739, 4760, 4870, 4884, 4901, 4910, 4918, 4921, 4943, 4952, 4961, 4968, 4978, 4991, 5007, 5019, 5030, 5040, 5060, 5072, 5088, 5092, 5100, 5116, 5148, 5151, 5178, 5197, 5204, 5212, 5290, 5295, 5299, 5309, 5350, 5357, 5360, 5364, 5369, 5374, 5391, 5395, 5399, 5408, 5422, 5445, 5468, 5473, 5477, 5484, 5516, 5523, 5529, 5532, 5538, 5551, 5563, 5579, 5586, 5607, 5617, 5671, 5674, 5686, 5689, 5703, 5711, 5721, 5752, 5756, 5762, 5767, 5780, 5783, 5790, 5795, 5835, 5838, 5849, 5856, 5861, 5877, 5886, 5903, 5914, 5918, 5927, 5949, 5966, 5977, 5981, 5995], 'highly': [17, 256, 548, 897, 1154, 4214, 4644], 'conserved': [18, 183, 257, 422, 898, 4740, 5878], 'α-sarcin/ricin': [19, 96, 258, 335, 551, 953, 1385, 3451, 4647, 4742, 5553, 5768], 'loop': [20, 98, 259, 337, 552, 954, 1387, 1704, 1742, 2358, 2552, 2754, 2790, 2902, 2912, 3453, 3479, 3633, 3643, 3897, 4032, 4115, 4498, 4512, 4539, 4569, 4648, 4744, 4879, 4963, 5009, 5032, 5062, 5077, 5094, 5120, 5206, 5376, 5555, 5589, 5713, 5770], 'of': [21, 40, 54, 83, 92, 99, 122, 129, 143, 161, 195, 211, 222, 260, 279, 293, 322, 331, 338, 361, 368, 382, 400, 434, 450, 461, 557, 641, 718, 738, 745, 768, 885, 908, 926, 932, 951, 1029, 1047, 1056, 1090, 1151, 1160, 1165, 1173, 1201, 1208, 1214, 1221, 1265, 1271, 1274, 1282, 1300, 1336, 1351, 1388, 1398, 1414, 1464, 1507, 1577, 1612, 1624, 1635, 1648, 1684, 1695, 1700, 1752, 1763, 1770, 1794, 1844, 1849, 1852, 1857, 1860, 1866, 1873, 1885, 1895, 1942, 2111, 2205, 2379, 2410, 2444, 2448, 2467, 2484, 2511, 2525, 2584, 2597, 2688, 2731, 2744, 2768, 2780, 2786, 2806, 2844, 2878, 2889, 2899, 2909, 2919, 3006, 3046, 3056, 3063, 3104, 3114, 3140, 3160, 3205, 3213, 3221, 3281, 3313, 3354, 3380, 3439, 3448, 3454, 3458, 3467, 3475, 3522, 3547, 3564, 3578, 3590, 3596, 3603, 3618, 3627, 3637, 3652, 3678, 3687, 3695, 3732, 3754, 3769, 3808, 3834, 3842, 3850, 3861, 3886, 3889, 3900, 3908, 3929, 4021, 4024, 4035, 4043, 4064, 4082, 4089, 4103, 4182, 4192, 4198, 4229, 4241, 4263, 4271, 4344, 4374, 4400, 4410, 4436, 4453, 4465, 4481, 4495, 4509, 4520, 4529, 4558, 4581, 4598, 4610, 4614, 4653, 4727, 4747, 4763, 4835, 4854, 4872, 4890, 4893, 4920, 4954, 5021, 5039, 5087, 5115, 5150, 5174, 5184, 5211, 5271, 5289, 5298, 5349, 5359, 5368, 5394, 5476, 5486, 5527, 5531, 5543, 5556, 5562, 5581, 5637, 5646, 5673, 5688, 5697, 5720, 5755, 5764, 5771, 5792, 5799, 5837, 5855, 5890, 5906, 5917, 5954, 5976, 5980], 'large': [23, 262, 555, 1650, 3219, 4651], 'ribosomal': [24, 212, 263, 451, 1048, 1651, 2213, 2366, 5907], 'RNA,': [25, 213, 264, 452], 'thereby': [26, 265], 'inhibiting': [27, 266], 'synthesis': [29, 268, 643], 'at': [30, 190, 269, 429, 644, 1098, 1257, 1787, 1936, 2293, 2387, 2486, 2575, 2649, 2709, 2799, 2927, 2955, 2973, 3059, 3153, 3156, 3209, 4394, 4429, 4454, 4574, 4599, 5126, 5135, 5383, 5885], 'elongation': [32, 271, 631], 'step.': [33, 272], 'Recently,': [34, 273], 'we': [35, 57, 274, 296, 1424, 1731, 2329, 4730, 5411, 5574, 5729], 'discovered': [36, 275], 'alanine': [38, 277, 1457, 1575, 3520, 3562, 3676, 4122, 4249, 4342, 5695], 'substitutions': [39, 278, 1200, 1458, 1576, 3677, 4123, 4250, 4343], 'active': [42, 86, 114, 145, 168, 192, 281, 325, 353, 384, 407, 431, 743, 1032, 1162, 1267, 1284, 1307, 1362, 1470, 1588, 1591, 1621, 1882, 3414, 3426, 3524, 3566, 3810, 4092, 4316, 4358, 4979, 5283, 5533, 5597, 5707, 5758, 5784, 5839, 5863, 5887], 'center': [43, 87, 115, 146, 193, 282, 326, 354, 385, 432, 1163, 1268, 1285, 1363, 1592, 1622, 3415, 3525, 3567, 3811, 4093, 4317, 4359, 4980, 5284, 5534, 5598, 5708, 5759, 5785, 5840, 5888], 'cleft': [44, 88, 116, 147, 194, 283, 327, 355, 386, 433, 1097, 1164, 1269, 1286, 1364, 1593, 1623, 3416, 3526, 3568, 3812, 4094, 4318, 4360, 4981, 5535, 5599, 5709, 5760, 5786, 5841, 5889], 'residues': [45, 142, 284, 381, 1034, 1150, 1159, 1270, 1281, 1365, 1511, 1578, 3417, 3460, 3490, 3527, 3569, 3638, 4345, 5526, 5530, 5836, 5896], 'significantly': [46, 285, 1438, 3829, 4326, 5003], 'impair': [47, 286], 'depurinating': [49, 288, 1059, 1451, 3767, 3806, 3840], 'and': [50, 73, 158, 180, 185, 197, 237, 289, 312, 397, 419, 424, 436, 476, 559, 634, 793, 893, 905, 920, 942, 1038, 1060, 1104, 1142, 1157, 1210, 1297, 1357, 1412, 1435, 1484, 1509, 1515, 1590, 1680, 1767, 1790, 1862, 1880, 1897, 1939, 2040, 2056, 2122, 2173, 2185, 2280, 2298, 2305, 2385, 2415, 2425, 2470, 2515, 2519, 2611, 2635, 2652, 2678, 2697, 2712, 2750, 2770, 2822, 2904, 2933, 2945, 2958, 2977, 3015, 3027, 3079, 3233, 3255, 3278, 3298, 3323, 3335, 3345, 3377, 3420, 3502, 3529, 3539, 3582, 3630, 3649, 3659, 3680, 3691, 3715, 3727, 3734, 3777, 3815, 3826, 3844, 3858, 3864, 3912, 3927, 4047, 4062, 4113, 4173, 4210, 4285, 4301, 4310, 4322, 4357, 4402, 4504, 4551, 4655, 4848, 4903, 4935, 4956, 4985, 4999, 5026, 5082, 5099, 5252, 5336, 5479, 5514, 5540, 5602, 5624, 5657, 5666, 5706, 5745, 5852, 5875, 5880, 5892, 5932, 5938, 5952, 5957, 6000, 6021], 'ribosome': [51, 290, 722, 1061, 1737], 'inhibitory': [52, 291, 723, 1062], 'activity': [53, 292, 1063, 2546, 2557, 2567, 2793, 3833, 4278, 4501, 4845], 'PAP.': [55, 294, 3756, 3836, 4335], 'Here': [56, 295], 'employed': [58, 297, 4768, 5730], 'site-directed': [59, 298, 1027, 1959, 4127, 5731], 'mutagenesis': [60, 299, 1028, 1960, 5732, 5982], 'combined': [61, 300, 5733], 'with': [62, 70, 301, 309, 630, 1360, 1430, 1456, 1502, 1574, 1609, 1739, 2228, 2374, 2422, 2427, 2478, 2606, 2619, 2685, 2783, 2809, 2896, 3186, 3366, 3444, 3469, 3494, 3508, 3544, 3600, 3611, 3645, 3684, 3698, 3730, 3752, 3762, 3848, 4107, 4121, 4216, 4238, 4248, 4268, 4333, 4341, 4372, 4492, 4535, 4566, 4851, 4967, 5037, 5112, 5434, 5567, 5635, 5694, 5734, 5742, 5972, 5989], 'standard': [63, 302, 4256, 5735], 'filter': [64, 303, 4257, 4864, 5736], 'binding': [65, 68, 91, 121, 160, 233, 304, 307, 330, 360, 399, 472, 1299, 1396, 1415, 1496, 1514, 1596, 1629, 2536, 2620, 2724, 2743, 2767, 2810, 2846, 2882, 2920, 3197, 3589, 3666, 3711, 3741, 3750, 3860, 3887, 3917, 4022, 4052, 4097, 4101, 4228, 4258, 4287, 4329, 4399, 4521, 4544, 4865, 4871, 4899, 4905, 4922, 4937, 4953, 4969, 5005, 5020, 5058, 5068, 5079, 5090, 5134, 5153, 5168, 5186, 5209, 5269, 5366, 5397, 5401, 5409, 5432, 5442, 5481, 5549, 5572, 5640, 5737, 5740, 5763, 5791, 5854, 5928, 5943], 'assays,': [66, 305, 2537, 5573, 5738], 'equilibrium': [67, 306, 2845, 3381, 5067, 5089, 5152, 5739], 'assays': [69, 308, 5433, 5482, 5741], 'Scatchard': [71, 310, 2827, 5157, 5301, 5743], 'analyses,': [72, 311, 5744], 'surface': [74, 313, 1417, 3045, 3097, 3167, 3179, 5593, 5746], 'plasmon': [75, 314, 1418, 5594, 5747], 'resonance': [76, 315, 1419, 3085, 5748], 'technology': [77, 316, 5749], 'to': [78, 94, 124, 153, 317, 333, 363, 392, 637, 734, 1292, 1343, 1366, 1383, 1460, 1598, 1630, 1802, 1889, 2322, 2475, 2616, 2756, 3001, 3135, 3217, 3250, 3270, 3302, 3308, 3463, 3486, 3553, 3585, 3587, 3592, 3622, 3663, 3667, 3707, 3713, 3745, 3914, 4049, 4076, 4196, 4288, 4363, 4444, 4589, 4738, 4769, 4876, 4900, 4960, 5006, 5029, 5059, 5070, 5091, 5203, 5373, 5380, 5398, 5405, 5443, 5463, 5547, 5550, 5577, 5718, 5750, 5766, 5794, 5847, 6002], 'elucidate': [79, 318, 5751], 'putative': [81, 320, 5753], 'role': [82, 217, 321, 456, 925, 4088, 5754, 5912], 'PAP': [85, 93, 123, 167, 196, 236, 324, 332, 362, 406, 435, 475, 541, 719, 740, 769, 786, 1031, 1091, 1166, 1353, 1401, 1572, 1625, 1772, 1886, 1898, 1921, 1932, 1954, 1998, 2010, 2043, 2602, 2772, 2891, 3012, 3489, 3512, 3591, 3723, 3852, 3866, 4091, 4105, 4119, 4185, 4202, 4246, 4264, 4282, 4339, 4377, 4405, 4414, 4472, 4484, 4550, 4562, 4618, 4636, 4859, 4873, 4896, 4958, 5023, 5042, 5103, 5106, 5130, 5146, 5190, 5213, 5272, 5352, 5371, 5429, 5449, 5459, 5544, 5583, 5620, 5676, 5691, 5723, 5757, 5765, 5793, 5862, 5891, 5931, 5946], 'in': [89, 119, 166, 207, 218, 328, 358, 405, 446, 457, 553, 730, 934, 947, 1043, 1051, 1058, 1305, 1468, 1512, 1607, 1744, 1760, 1809, 1827, 1907, 2047, 2434, 2572, 2581, 2728, 2802, 2916, 2961, 3066, 3108, 3119, 3237, 3435, 3465, 3483, 3505, 3760, 4069, 4095, 4161, 4251, 4298, 4369, 4432, 4517, 4577, 4649, 4723, 4883, 4913, 5155, 5385, 5390, 5571, 5761, 5789, 5860, 5902, 5913], 'stem': [97, 336, 1386, 1703, 1741, 3452, 3478, 4743, 5008, 5093, 5119, 5375, 5554, 5588, 5712, 5769], 'rRNA.': [100, 130, 238, 339, 369, 477, 1631, 3455, 3470, 3509, 3668, 5560, 5772, 5800, 5933], 'Our': [101, 340, 1137, 1253, 3383, 3471, 5773], 'findings': [102, 341, 1603, 5265, 5774, 5968], 'presented': [103, 342, 1604, 5474, 5967], 'herein': [104, 343, 1605], 'provide': [105, 344, 5775], 'experimental': [106, 345, 1602, 3268, 5776], 'evidence': [107, 346, 1145, 1427, 5520, 5777], 'besides': [109, 348, 1147, 5522, 5779], 'catalytic': [111, 199, 209, 220, 350, 438, 448, 459, 949, 1045, 1149, 1219, 3437, 3670, 3821, 4305, 4355, 4992, 5280, 5524, 5608, 5704, 5781, 5894, 5904, 5915], 'site,': [112, 351], 'also': [117, 177, 356, 416, 1093, 1168, 1492, 2739, 5412, 5545, 5630, 5787, 5872], 'participates': [118, 357, 5788], 'target': [126, 227, 365, 466, 1226, 3450, 4108, 4741, 5469, 5552, 5796, 5922], 'tetraloop': [127, 366, 1879, 2269, 2341, 3594, 5425, 5797], 'structure': [128, 367, 767, 1647, 1683, 1738, 1743, 1903, 3595, 5798], 'These': [131, 370, 3757, 4156, 5264, 5454, 5801], 'results': [132, 371, 4970, 5475, 5802], 'extend': [133, 372, 5803], 'our': [134, 373, 1613, 1696, 1745, 4083, 4724, 5487, 5804], 'recent': [135, 374, 731, 1138, 1614, 5805], 'modeling': [136, 375, 1254, 1615, 3385, 4084, 5488, 5806], 'studies,': [137, 376, 1616], 'which': [138, 377, 1617, 1992, 2264, 2758, 3169, 3181, 3430, 3531, 3572, 3635, 5048, 5832, 5984], 'predicted': [139, 378, 3462, 3584, 3662, 3706, 3744, 5833], 'could,': [148, 387, 1287, 5842], 'via': [149, 388, 1288, 3541, 5467, 5843], 'electrostatic': [150, 389, 1289, 5844], 'interactions,': [151, 390, 1290, 4729, 5845], 'contribute': [152, 391, 1291, 3586, 5546, 5846], 'both': [154, 393, 1293, 1433, 3657, 5848], 'correct': [156, 395, 1295, 5850], 'orientation': [157, 396, 1296, 1463, 5851], 'stable': [159, 398, 1298, 5853], 'substrate': [163, 402, 929, 1110, 1302, 1466, 2268, 2340, 3859, 4290, 5075, 5137, 5424, 5857], 'RNA': [164, 403, 1049, 1303, 1442, 1477, 1499, 1599, 2214, 2367, 2499, 2550, 2752, 2788, 3018, 4291, 4328, 4735, 4756, 5010, 5033, 5095, 5121, 5207, 5268, 5377, 5465, 5590, 5639, 5714, 5858, 5908], 'molecules': [165, 404, 1112, 1354, 5859], 'site': [169, 200, 408, 439, 1308, 1471, 1480, 1589, 1883, 3427, 3671, 3742, 3822, 4306, 4356, 4993, 5281, 5525, 5609, 5705, 5782, 5864, 5895], 'pocket.': [170, 409, 5865], 'The': [171, 410, 715, 763, 880, 1334, 1395, 1601, 1632, 1750, 1776, 1798, 1824, 1901, 2084, 2163, 2189, 2203, 2286, 2360, 2429, 2508, 2641, 2660, 2705, 2722, 2812, 2870, 2922, 2951, 3021, 3033, 3094, 3276, 3311, 3329, 3343, 3510, 3641, 3669, 3804, 3982, 4018, 4117, 4206, 4542, 4833, 5194, 5286, 5596, 5644, 5866], 'insights': [172, 411, 5867], 'gained': [173, 412, 727, 5868], 'this': [175, 414, 1345, 1828, 3090, 3487, 4836, 4904, 5435, 5870], 'study': [176, 415, 1341, 4726, 5871], 'explain': [178, 417, 5873], 'why': [179, 418, 5874], 'how': [181, 420, 5876], 'charged': [184, 423, 5879], 'polar': [186, 425, 1158, 5881], 'side': [187, 426, 1033, 5882], 'chains': [188, 427, 5883], 'located': [189, 428, 3533, 5536, 5884], 'certain': [198, 437, 5893], 'residues,': [201, 440, 889, 1203], 'do': [203, 442, 5898], 'not': [204, 443, 2337, 3432, 4386, 4939, 5420, 5439, 5899], 'directly': [205, 444, 945, 1041, 3433, 4078, 5900], 'participate': [206, 445, 1042, 3504, 5901], 'deadenylation': [210, 449, 950, 1046, 5905], 'play': [214, 453, 5909], 'critical': [216, 455, 1169, 5911], 'removal': [221, 460, 1220, 5916], 'rRNA': [228, 467, 556, 933, 1111, 1174, 1227, 1275, 1371, 1391, 1513, 1753, 1896, 2417, 2430, 2516, 2542, 2749, 3548, 3714, 3740, 3749, 3766, 3805, 3894, 4029, 4096, 4112, 4234, 4276, 4368, 4392, 4422, 4652, 4751, 4764, 5185, 5396, 5923, 5942], 'substrates': [229, 468, 1228, 5466, 5570, 5924], 'by': [230, 469, 1026, 1347, 1781, 1926, 2089, 2282, 2311, 2372, 2406, 2420, 2494, 2726, 2776, 2885, 2943, 3029, 3052, 3100, 3201, 3317, 3351, 3519, 3561, 4098, 4175, 4463, 4608, 4909, 4942, 5276, 5387, 5592, 5925], 'affecting': [231, 470, 5926], 'interactions': [234, 473, 3443, 3468, 3610, 4102, 4772, 5929], 'between': [235, 474, 1101, 3009, 3295, 5537, 5930], 'pokeweed': [478], 'BSA,': [483, 2634, 2757, 4487, 4929, 5047], 'bovine': [484, 2102], 'serum': [485, 2103, 2170, 4219], 'albumin': [486, 2104], 'high': [487], 'pressure': [488], 'liquid': [489], 'chromatograph': [490], 'oligonucleotide': [491], 'streptavidin': [492, 3048], '(PAP)1': [496], '30-kDa': [499], '(RIP)': [502], 'isolated': [503], 'leaves': [506], 'ofPhytolacca': [507], 'americana': [508], '(pokeweed': [509], 'plant)': [510], '(1Irvin': [511], 'J.D.': [512, 525, 529, 583, 599, 651, 665, 682, 750, 806, 808, 825, 962, 964, 993, 1524, 1526, 1545, 4679, 4695], 'Arch.': [513, 530, 668], 'Biochem.': [514, 531, 669, 705, 854], 'Biophys.': [515, 532, 670], '1975;': [516, 707], '169:': [517], '522-528Crossref': [518], 'PubMed': [519, 536, 577, 591, 605, 623, 657, 674, 692, 710, 758, 815, 833, 858, 875, 971, 1003, 1019, 1083, 1132, 1194, 1248, 1329, 1533, 1552, 1564, 1675, 1725, 1984, 2034, 2079, 2158, 2255, 2867, 3406, 3799, 4011, 4151, 4673, 4687, 4701, 4719, 4787, 4806, 4828, 5509, 5827], 'Scopus': [520, 537, 578, 592, 606, 658, 675, 711, 759, 816, 834, 859, 876, 972, 1020, 1084, 1133, 1195, 1249, 1330, 1534, 1553, 1565, 1676, 1726, 1985, 2035, 2080, 2159, 2256, 2839, 3407, 3800, 4012, 4152, 4674, 4688, 4702, 4788, 4807, 4829, 5510, 5828], '(163)': [521], 'Google': [522, 539, 580, 594, 608, 624, 660, 677, 693, 713, 761, 818, 836, 861, 878, 974, 1004, 1022, 1086, 1135, 1197, 1251, 1332, 1536, 1555, 1567, 1678, 1728, 1917, 1987, 2037, 2082, 2161, 2258, 2841, 2868, 3409, 3802, 4014, 4154, 4676, 4690, 4704, 4720, 4790, 4809, 4831, 5512, 5830], 'Scholar,': [523, 581, 595, 609, 661, 678, 694, 819, 837, 862, 975, 1005, 1537, 4677, 4691, 4705, 4791, 4810], '2Irvin': [524], 'Kelly': [526], 'T.': [527, 4812], 'Robertus': [528, 807, 963, 992, 1525, 1544], '1980;': [533, 686], '200:': [534], '418-425Crossref': [535], '(105)': [538], 'Scholar).': [540, 625, 714, 762, 879, 1023, 1087, 1136, 1198, 1252, 1333, 1568, 1729, 1823, 1918, 1988, 2038, 2083, 2162, 2259, 2869, 3803, 4155, 4721, 4832], 'removes': [543, 4638], 'an': [544, 638, 935, 1863, 2398, 3535, 4188, 5388], 'conserved,': [549, 1155, 4645], 'surface-exposed,': [550, 4646], 'prokaryotic': [558, 4654], 'eukaryotic': [560, 1393, 4656, 4748, 5557], 'ribosomes': [561, 627, 4657], '(3Hartley': [562, 4658], 'M.R.': [563, 4659], 'Legname': [564, 865, 4660], 'G.': [565, 702, 864, 866, 2830, 4661], 'Osborn': [566, 4662], 'R.': [567, 991, 4663, 4816], 'Chen': [568, 4664], 'Z.': [569, 4665], 'Lord': [570, 4666], 'J.M.': [571, 4667], 'FEBS': [572, 600, 652, 4668, 4696], 'Lett.': [573, 601, 653, 4669, 4697], '1991;': [574, 4670, 4803], '290:': [575, 4671], '65-68Crossref': [576, 4672], '(125)': [579, 4675], '4Irvin': [582, 4678], 'Uckun': [584, 751, 826, 1012, 1070, 1119, 1181, 1235, 1316, 1712, 1971, 2021, 2071, 2150, 2242, 3393, 3786, 3998, 4138, 4680, 5496, 5814], 'F.M.': [585, 752, 827, 1013, 1071, 1120, 1182, 1236, 1317, 1713, 1972, 2022, 2072, 2151, 2243, 2854, 3394, 3787, 3999, 4139, 4681, 5497, 5815], 'Pharmacol.': [586, 753, 4682], 'Ther.': [587, 754, 4683], '1992;': [588, 755, 4684], '55:': [589, 756, 4685], '279-302Crossref': [590, 757, 4686], '(104)': [593, 760, 4689], '5Dallal': [596, 4692], 'J.A.': [597, 649, 4693], 'Irvin': [598, 650, 664, 681, 805, 824, 961, 1523, 4694], '1978;': [602, 654, 4698], '89:': [603, 655, 4699], '257-259Crossref': [604, 656, 4700], '(33)': [607, 659, 4703], '6Endo': [610, 4706], 'Y.': [611, 2833, 4707], 'Tsurugi': [612, 4708], 'K.': [613, 4709], 'J.': [614, 683, 706, 809, 841, 853, 869, 965, 994, 1072, 1121, 1183, 1237, 1318, 1527, 1543, 1666, 1714, 1911, 1973, 2023, 2244, 3395, 3788, 4000, 4140, 4710, 4817, 5498, 5816, 6008], 'Biol.': [615, 684, 811, 967, 995, 1073, 1122, 1184, 1238, 1319, 1529, 1548, 1715, 1974, 2024, 2245, 3396, 3789, 4001, 4141, 4711, 4818, 5499, 5817], 'Chem.': [616, 685, 996, 1074, 1123, 1185, 1239, 1320, 1716, 1975, 2025, 2246, 3397, 3790, 4002, 4142, 4712, 4819, 5500, 5818], '1988;': [617, 1914, 4713], '263:': [618, 4714], '8735-8739Abstract': [619, 4715], 'Full': [620, 689, 1000, 1078, 1080, 1127, 1129, 1189, 1191, 1243, 1245, 1324, 1326, 1720, 1722, 1979, 1981, 2029, 2031, 2250, 2252, 3401, 3403, 3794, 3796, 4006, 4008, 4146, 4148, 4716, 4823, 4825, 5504, 5506, 5822, 5824], 'Text': [621, 690, 1001, 1079, 1081, 1128, 1130, 1190, 1192, 1244, 1246, 1325, 1327, 1721, 1723, 1980, 1982, 2030, 2032, 2251, 2253, 3402, 3404, 3795, 3797, 4007, 4009, 4147, 4149, 4717, 4824, 4826, 5505, 5507, 5823, 5825], 'PDF': [622, 691, 1002, 1082, 1131, 1193, 1247, 1328, 1724, 1983, 2033, 2254, 3405, 3798, 4010, 4150, 4718, 4827, 5508, 5826], 'PAP-depurinated': [626], 'cannot': [628], 'interact': [629, 3540], 'factors': [632], '1': [633, 2627, 2686, 2931, 3130], '2,': [635], 'leading': [636], 'irreversible': [639], 'inhibition': [640], 'translocation': [646], 'step': [647], '(5Dallal': [648], '7Obrig': [662], 'T.G.': [663, 2856], 'Hardesty': [666], 'B.': [667, 985], '1973;': [671], '155:': [672], '278-289Crossref': [673], '(85)': [676], '8Gessner': [679], 'S.L.': [680], '255:': [687], '3251-3253Abstract': [688], '9Montanaro': [695], 'L.': [696, 6014], 'Sperti': [697], 'S.': [698, 4782, 4801, 6017], 'Mattioli': [699], 'A.': [700, 1539, 2862, 4774, 4783, 4802, 4814], 'Testoni': [701], 'Stirpe': [703], 'F.': [704, 1009, 1065, 1114, 1176, 1230, 1311, 1707, 1966, 2016, 2062, 2141, 2237, 3388, 3781, 3993, 4133, 5491, 5809], '146:': [708], '127-131Crossref': [709], '(122)': [712], 'therapeutic': [716], 'potential': [717], 'as': [720, 741, 1375, 1377, 1490, 1962, 2051, 2058, 2106, 2137, 2182, 2196, 2233, 2761, 2824, 2850, 2914, 2966, 3111, 3190, 3422, 3424, 3817, 3819, 4129, 4167, 4312, 4314, 4514, 4526, 4886, 4888, 4988, 4990, 5051, 5292, 5604, 5606, 5944], 'anticancer': [724], 'agent': [725], 'has': [726, 912, 1993, 3172, 3516, 3558, 3606, 3675], 'considerable': [728], 'interest': [729], 'years': [732], 'due': [733, 5973], 'clinical': [736], 'use': [737], 'native': [739, 1990, 2009], 'moiety': [744], 'immunoconjugates': [746], 'against': [747], 'cancer': [748], '(4Irvin': [749], 'resolved': [764, 2116], 'three-dimensional': [765], 'x-ray': [766], 'revealed': [770, 1094], 'could': [774, 1108], 'be': [775, 1890, 5381, 5970, 6003], 'divided': [776], 'into': [777, 2530], 'three': [778, 2683, 3930, 4065, 4252, 4456, 4601, 5627], 'distinct': [779], 'domains:': [780], 'N-terminal': [782], 'domain': [783, 790, 796, 882, 1106], '(residues': [784, 791, 797], '1–69,': [785], 'numbering),': [787], 'central': [789, 881, 1103, 5539], '70–179),': [792], 'C-terminal': [795, 1105, 3577, 5541], '180–262)': [798], '(10Monzingo': [799, 955, 1517], 'A.F.': [800, 956, 981, 1518], 'Collins': [801, 957, 1519], 'E.J.': [802, 958, 1520], 'Ernst': [803, 959, 982, 1521], 'S.R.': [804, 960, 983, 1522], 'Mol.': [810, 870, 966, 1528, 1546, 1912], '1993;': [812, 968, 1530], '233:': [813, 969, 1531], '705-715Crossref': [814, 970, 1532], '(113)': [817, 973, 1535], '11Kurinov': [820], 'I.V.': [821, 1007, 1069, 1118, 1180, 1234, 1315, 1711, 1970, 2020, 2241, 3392, 3785, 3997, 4137, 5495, 5813], 'Myers': [822, 2859], 'D.E.': [823, 2860, 4795], 'Protein': [828, 1014, 2073, 2094, 2152], 'Sci.': [829, 1015, 2835, 4780, 4799], '1999;': [830, 1016, 2076, 2155], '8:': [831, 1017], '1765-1772Crossref': [832], '(53)': [835], '12Ago': [838], 'H.': [839, 843, 1734], 'Kataoka': [840], 'Tsuge': [842], 'Habuka': [844], 'N.': [845, 1662, 2832], 'Inagaki': [846], 'E.': [847, 987, 1944, 2048, 2208, 2361, 4163, 4424], 'Noma': [848], 'M.': [849, 851, 6011], 'Miyano': [850], 'Eur.': [852], '1994;': [855], '225:': [856], '369-374Crossref': [857], '(28)': [860, 1085, 1134, 1196, 1250, 1331, 1727, 1986, 2036, 2257, 3408, 3801, 4013, 4153, 5511, 5829], '13Bravi': [863], 'Chan': [867], 'A.W.': [868], 'Graph.': [871], '1995;': [872, 4820], '13:': [873], '83-88Crossref': [874], '(6)': [877], 'accommodates': [883], 'all': [884, 3253, 4468, 4613], 'enzyme-active': [887], '(catalytic)': [888], 'Tyr72,': [890, 1035], 'Tyr123,': [891, 1036], 'Glu176,': [892, 1037], 'Arg179,': [894], 'are': [896, 944, 1167, 1584, 1606, 2006, 3431, 3532, 3573, 3583, 3736, 3759, 4351, 5700, 5998], 'among': [899], 'RIPs.': [901], 'Based': [902], 'on': [903, 2117, 2276, 2395, 2655, 3043, 3168, 3180, 3229, 3534, 3575, 5196], 'structural': [904], 'biochemical': [906, 1144, 5519], 'evaluation': [907], 'several': [909], 'RIPs,': [910], 'it': [911], 'been': [913, 3173, 3184, 3517, 3559, 4767], 'proposed': [914], 'amino': [917, 1933, 1995, 2002], 'acids': [918, 1934], 'Tyr72': [919], 'Tyr123(PAP': [921], 'numbering)': [922], 'have': [923, 1092, 2000, 3664, 3708, 3746, 4765], '"sandwiching"': [927], 'ring': [931, 3694], 'energetically': [936], 'favorable': [937], 'stacking': [938], 'conformation,': [939, 5992], 'while': [940], 'Arg179': [941, 1039, 1508], 'Glu176': [943], 'engaged': [946], '14Montfort': [976], 'W.': [977], 'Villafranca': [978], 'J.E.': [979], 'Monzingo': [980], 'Katzin': [984], 'Rutenber': [986], 'Xuong': [988], 'N.H.': [989], 'Hamlin': [990], '1987;': [997], '262:': [998], '5398-5403Abstract': [999], '15Kurinov': [1006], 'Rajamohan': [1008, 3877, 3989], 'Venkatachalam': [1010], 'T.K.': [1011], '2399-2405Crossref': [1018], '(20)': [1021], 'Alanine': [1024, 1199], 'substitution': [1025, 3521, 3563, 5696], 'resulted': [1050], 'greater': [1052], 'than': [1053, 2008, 3831, 5356, 5616, 5670], '3': [1054, 2630, 3076, 4236, 4753, 4839, 5247], 'logs': [1055], 'loss': [1057, 1207, 1213], '(16Rajamohan': [1064, 1113, 1175, 1229, 1310, 1706, 1965, 2015, 2236, 3387, 3780, 3992, 4132, 5490, 5808], 'Pugmire': [1066, 1115, 1177, 1231, 1312, 1708, 1967, 2017, 2238, 3389, 3782, 3994, 4134, 5492, 5810], 'M.J.': [1067, 1116, 1178, 1232, 1313, 1709, 1968, 2018, 2239, 3390, 3783, 3995, 4135, 5493, 5811], 'Kurinov': [1068, 1117, 1179, 1233, 1314, 1710, 1969, 2019, 2240, 3391, 3784, 3996, 4136, 5494, 5812], '2000;': [1075, 1124, 1186, 1240, 1321, 1561, 1672, 1717, 1976, 2026, 2247, 3398, 3791, 4003, 4143, 5501, 5819], '275:': [1076, 1125, 1187, 1241, 1322, 1718, 1977, 2027, 2248, 3399, 3792, 4004, 4144, 5502, 5820], '3382-3390Abstract': [1077, 1126, 1188, 1242, 1323, 1719, 1978, 2028, 2249, 3400, 3793, 4005, 4145, 5503, 5821], 'Structural': [1088], 'studies': [1089, 1139, 1255, 3386, 4085, 5069, 5489, 5807], 'prominent': [1096], 'interface': [1100], 'accommodate': [1109], 'provided': [1140, 5515], 'unprecedented': [1141], 'direct': [1143, 1426, 5518], 'that,': [1146, 1428, 5521], 'PAP,': [1152, 1432, 1991, 5223, 5528], 'charged,': [1156], 'for': [1170, 1262, 1403, 1441, 1448, 1476, 1498, 1628, 1832, 1839, 2200, 2355, 2390, 2397, 2578, 2645, 2657, 2715, 2796, 2930, 3244, 3252, 3293, 3306, 3881, 4016, 4426, 4548, 4554, 4571, 5097, 5123, 5167, 5189, 5220, 5228, 5235, 5242, 5249, 5257, 5308, 5316, 5322, 5328, 5334, 5341, 5428, 5585, 5710, 6024], 'PAP-mediated': [1171, 1272], 'depurination': [1172, 1273, 1516, 3438], 'these': [1202, 3459], 'especially': [1204, 5993], '69NN70': [1205], '(191-fold': [1206], 'activity)': [1209], '90FND92': [1211], '(352-fold': [1212], 'activity),': [1215], 'substantially': [1216], 'impaired': [1217, 1439, 3665, 4327], 'aimed': [1256], 'understanding': [1258], 'molecular': [1260, 1633, 3473, 4190], 'basis': [1261], 'contribution': [1264], 'prompted': [1276], 'hypothesis': [1278, 1346], 'molecule': [1304, 1773], 'pocket': [1309], 'purpose': [1335], 'present': [1338, 1425, 4725], 'structure-function': [1339], 'relationship': [1340], 'was': [1342, 1641, 1754, 1778, 1800, 1904, 1924, 2115, 2165, 2191, 2218, 2274, 2291, 2309, 2348, 2370, 2404, 2418, 2432, 2473, 2492, 2528, 2614, 2643, 2663, 2681, 2707, 2713, 2733, 2759, 2774, 2815, 2848, 2873, 2883, 2925, 2937, 2953, 2964, 2971, 2999, 3024, 3039, 3092, 3098, 3225, 3315, 3705, 4442, 4473, 4545, 4587, 4619, 4841, 4861, 4906, 4938, 5049, 5716], 'test': [1344], 'evaluating': [1348], 'ability': [1350, 4330], 'recombinant': [1352, 1571, 1997, 2042, 2598, 3862, 4104, 4118, 4200, 4208, 4242, 4338, 4404, 4483, 4858, 5022, 5102, 5145, 5221, 5582, 5619, 5690], 'FLP-4': [1355, 1434, 3514, 3658, 3814, 4320, 4983, 5229, 5601, 5647], '(69AA70)': [1356, 3515, 4321, 4984], 'FLP-7': [1358, 1436, 3556, 3660, 3816, 4323, 4986, 5236, 5603, 5652], '(90AAA92)': [1359, 3557, 4324], 'mutated': [1361, 5400], 'bind': [1367, 4364, 4387, 5462], '23': [1368, 2210, 2363, 2539, 2746, 3891, 4026, 4231, 4273, 4365, 4389, 4419, 4974], 'S/16': [1369, 2211, 2364, 2540, 2747, 3892, 4027, 4232, 4274, 4293, 4366, 4390, 4420, 4975], 'S': [1370, 1390, 2212, 2365, 2541, 2748, 3893, 4028, 4111, 4233, 4275, 4294, 4367, 4391, 4421, 4750, 4976, 5559], 'Escherichia': [1373, 1761], 'coli': [1374, 1945, 2049, 2209, 2362, 4164, 4425], 'well': [1376, 3423, 3818, 4313, 4887, 4989, 5605], 'synthetic': [1379, 1405], 'model': [1380, 1634, 1691, 1699, 1799, 3292, 3474, 4734], 'oligoribonucleotide': [1381, 1406, 2262, 2287, 2514, 4837, 5138], 'corresponding': [1382, 4737], '28': [1389, 4749, 5558], 'ribosomes.': [1394], 'affinities': [1397, 5645], 'mutant': [1400, 2041, 2601, 2771, 3011, 3511, 3672, 3865, 4157, 4184, 4211, 4245, 4403, 4413, 4561, 4857, 4957, 5101, 5351, 5370, 5448, 5610, 5628, 5692, 5935, 5958, 5987, 5996], 'proteins': [1402, 1999, 2044, 2086, 2603, 2773, 2895, 3013, 3116, 4106, 4158, 4186, 4212, 4247, 4265, 4415, 4563, 4860, 4959, 5107, 5131, 5214, 5353, 5372, 5450, 5460, 5584, 5629, 5936, 5959, 5988, 5997], 'were': [1407, 1488, 1887, 1956, 2045, 2087, 2096, 2517, 2570, 2604, 2981, 3117, 3151, 3199, 3235, 3248, 3259, 3263, 3284, 3332, 3338, 3349, 3372, 3461, 3661, 3743, 3828, 3846, 3874, 3902, 3986, 4037, 4124, 4159, 4213, 4266, 4361, 4416, 4490, 4564, 5110, 5215, 5303, 5354, 5663, 5960], 'calculated': [1408, 2874, 3350, 3373], 'rate': [1410, 3062, 3159, 3212, 3347, 3375], 'constants': [1411, 3348, 3376], 'analysis': [1413, 2843, 3379], 'using': [1416, 1806, 1958, 2101, 2132, 2167, 2193, 2220, 2296, 2350, 2717, 2983, 3188, 3359, 4126, 4255, 4863, 4972, 5568], '(SPR)': [1420], 'biosensor': [1421, 2994], 'technology.': [1422], 'Here,': [1423], 'compared': [1429, 4332, 4951], 'wild-type': [1431, 1920, 2599, 2769, 2890, 3755, 3835, 3851, 3863, 4201, 4209, 4243, 4281, 4334, 4376, 4401, 4411, 4482, 4549, 4559, 4855, 4955, 5041, 5098, 5222, 5310, 5361, 5446, 5618, 5675, 5722, 5945, 5956], 'exhibit': [1437, 5440], 'affinity': [1440, 1475, 1497, 1597, 3712, 5580, 5672, 5687], 'substrates,': [1443, 1500], 'providing': [1444], 'cogent': [1446], 'explanation': [1447], 'their': [1449, 5464, 5638], 'reduced': [1450, 1474, 1495, 5004, 5275, 5365], 'activity.': [1452, 2202, 3718], 'Similarly,': [1453], 'FLP-9': [1454, 3673, 3704, 4995, 5243, 5611, 5658], '(122AA123)': [1455, 3674], 'expected': [1459], 'affect': [1461, 3623], 'showed': [1472, 1493, 1594, 4283, 5056], 'markedly': [1473, 1494, 5274], 'targets.': [1478], 'Catalytic': [1479], 'mutants': [1481, 1573, 1955, 3724, 3813, 4120, 4307, 4319, 4340, 4994, 5024, 5600], 'FLP-12': [1482, 3775, 4997, 5250], '(Ala179)': [1483], 'FLP-13': [1485, 3778, 5000, 5258, 5342], '(Ala208),': [1486, 5001], 'included': [1489, 1830, 2760, 5050], 'controls,': [1491], 'consistent': [1501, 5634], 'previously': [1504, 1964, 2060, 2139, 2235, 2852, 4130], 'established': [1505], 'importance': [1506], 'Trp208': [1510], '17Frankel': [1538], 'Welsh': [1540], 'P.': [1541, 1664], 'Richardson': [1542], 'Cell.': [1547], '1990;': [1549], '10:': [1550], '6257-6263Crossref': [1551], '(97)': [1554], 'Scholar,18Gu': [1556], 'Y-J.': [1557], 'Xia': [1558], 'Z-X.': [1559], 'Proteins.': [1560], '39:': [1562], '37-46Crossref': [1563], '(21)': [1566], 'By': [1569, 3719, 3837, 4336, 5017, 5684], 'comparison,': [1570, 3838, 4337, 5018, 5685], '28KD29': [1579, 4346], '(FLP-1)': [1580, 4347], 'and111SR112': [1581, 4348], '(FLP-8)': [1582, 4349], 'distant': [1585, 3737, 4352, 5701], 'normal': [1595, 3748], 'substrates.': [1600], 'accordance': [1608, 4966], 'predictions': [1611, 3758, 4081, 5485], 'suggested': [1618], 'important': [1627], 'PAP-ribosome': [1637], '(large': [1638], 'subunit)': [1639], 'complex': [1640, 1686, 1705, 1748, 3480], 'derived': [1642, 3903, 4038], '2.4-Å': [1645], 'crystal': [1646, 1682], 'subunit': [1652, 1736], 'Haloarcula': [1654], 'marismortui(Protein': [1655], 'Data': [1656], 'Bank': [1657], 'access': [1658], 'code': [1659, 1688], '1FFK)': [1660], '(19Ban': [1661], 'Nissen': [1663], 'Hansen': [1665], 'Moore': [1667], 'P.B.': [1668], 'Steitz': [1669], 'T.A.': [1670], 'Science.': [1671], '289:': [1673], '905-920Crossref': [1674], '(2799)': [1677], 'Scholar)': [1679, 2842, 3410, 4015, 5513], 'PAP-nucleotide': [1685], '(access': [1687], '1pag).': [1689], 'This': [1690, 2344], 'represents': [1692], 'refinement': [1694], 'recently': [1697, 3764], 'published': [1698], 'PAP-rRNA': [1702], 'First,': [1730], 'superimposed': [1732], 'marismortui': [1735], 'previous': [1746, 3384], 'PAP-RNA': [1747, 3477], 'model.': [1749], 'conformation': [1751, 3626], 'adjusted': [1755, 1780, 2474, 2615, 3249], 'around': [1756], 'A2697(A2660': [1759], 'coli)': [1762], 'GAGA': [1765, 2271], 'tetraloop,': [1766], 'rest': [1769, 1894], 'remains': [1774], 'unchanged.': [1775], 'adenosine': [1777], 'manually': [1779], '38°': [1783], 'degree': [1784], 'out-flipping': [1785], 'rotation': [1786, 1856], 'C5′-position': [1789], '5.4-Å': [1792], 'translation': [1793, 1848], 'ring.': [1797], 'used': [1801, 1826, 2330, 3000, 4731, 5413], 'perform': [1803], 'fixed': [1804], 'docking': [1805, 1829, 1875], 'Dockingmodule': [1808], 'InsightII': [1810, 1815], 'employing': [1811], 'CVFF': [1813], '(29MSI,': [1814], 'User': [1816], 'Guide,': [1817], '1991,': [1818], 'MSI,': [1819], 'San': [1820], 'Diego,': [1821], 'CA.Google': [1822], 'parameters': [1825, 3005], 'searching': [1831], 'five': [1833], 'unique': [1834], 'structures:': [1835], '1000': [1836], 'minimization': [1837, 1871], 'steps': [1838, 1872], 'each': [1840, 2703, 2736, 4476, 4622, 5144], 'structure,': [1841], 'energy': [1842, 1864], 'range': [1843, 5173], '10.0': [1845], 'kcal/mol,': [1846], 'maximum': [1847, 1855, 3884, 4019, 5073, 5205], 'ligand': [1851, 1859], '3.0': [1853], 'Å,': [1854], '10°,': [1861], 'tolerance': [1865], '1500': [1867], 'kcal/mol.': [1868], 'During': [1869], 'procedure,': [1876], 'only': [1877], 'residue': [1884, 3428], 'allowed': [1888], 'flexible,': [1891], 'whereas': [1892], 'remained': [1899], 'fixed.': [1900], 'refined': [1902, 3472], 'then': [1905, 2653, 3264], 'analyzed': [1906, 2088, 4174], 'CHAIN': [1908], '(20Sack': [1909], 'J.S.': [1910], 'Graphics.': [1913], '6:': [1915], '244-249Crossref': [1916], 'Recombinant': [1919, 5105], 'construct': [1922], '(pBS-PAP)': [1923], 'obtained': [1925, 4971], 'subcloning': [1927], 'PAP-I': [1929], 'gene': [1930], 'encoding': [1931], '22–313': [1935], 'BamHI': [1938], 'HindIII': [1940], 'sites': [1941, 5187], 'expression': [1946], 'vector,': [1947], 'pBluescript': [1948], 'SK−': [1949], '(Stratagene,': [1950], 'La': [1951], 'Jolla,': [1952], 'CA).': [1953], 'constructed': [1957, 4125], 'techniques': [1961], 'described': [1963, 2059, 2138, 2234, 2353, 2825, 2851, 2967, 4131], 'Unlike': [1989], '262': [1994], 'acids,': [1996], '292': [2001], 'acids.': [2003], 'Therefore,': [2004, 3456, 3656, 3703, 4722, 5363], 'they': [2005], 'larger': [2007], '(33': [2011, 4874], 'versus': [2012], '29': [2013], 'kDa)': [2014], 'Wild-type': [2039], 'expressed': [2046, 4160, 4525], 'MV1190': [2050], 'inclusion': [2052, 4168], 'bodies,': [2053, 4169], 'isolated,': [2054], 'solubilized,': [2055, 4171], 'refolded,': [2057, 4172], '(21Rajamohan': [2061, 2140], 'Engstrom': [2063, 2142], 'C.R.': [2064, 2143], 'Denton': [2065, 2144], 'T.J.': [2066, 2145], 'Engen': [2067, 2146], 'L.A.': [2068, 2147], 'Kourinov': [2069, 2148], 'I.': [2070, 2149], 'Expression': [2074, 2153], 'Purif.': [2075, 2154], '16:': [2077, 2156], '359-368Crossref': [2078, 2157], '(36)': [2081, 2160], 'refolded': [2085, 4207, 5955], 'SDS-12%': [2090, 2119], 'polyacrylamide': [2091, 2120], 'gel': [2092, 2100, 2121, 4177], 'electrophoresis.': [2093], 'concentrations': [2095, 2785, 2906, 4240, 4409, 4506, 4557, 4892, 5114, 5139], 'determined': [2097, 2725, 2775, 4462, 4607, 5293], '(BSA)': [2105], 'standard.': [2108], 'One': [2109], 'μg': [2110], 'samples': [2114, 2782, 3148, 3234, 4466, 4611], 'transferred': [2123], 'onto': [2124, 3163, 3176], 'polyvinylidene': [2126], 'difluoride': [2127], 'membrane': [2128, 2164, 2668, 2680, 2706, 2952, 2963], '(Roche': [2129, 2215, 2302, 2318, 2501], 'Molecular': [2130, 2216, 2303, 2319, 2502], 'Biochemicals)': [2131, 2217, 2320, 2503], 'Bio-Rad': [2134], 'transblot': [2135], 'apparatus,': [2136], 'immunoblotted': [2166], 'rabbit': [2168], 'anti-PAP': [2169, 4218], '(1:2000': [2171], 'dilution)': [2172, 2181], 'horseradish': [2174], 'peroxidase-conjugated': [2175], 'goat': [2176], 'anti-rabbit': [2177], 'IgG': [2178], '(Sigma;': [2179], '1:1000': [2180], 'primary': [2184, 3356], 'secondary': [2186], 'antibodies,': [2187], 'respectively.': [2188, 2522], 'blot': [2190], 'developed': [2192], '3,3′-diaminobenzidine': [2194], '(Sigma)': [2195], 'colorimetric': [2198], 'indicator': [2199], 'peroxidase': [2201], 'release': [2204], 'measured': [2219, 2965, 5169], 'HPLC': [2221], '(Hewlett': [2222], 'Packard,': [2223], 'Palo': [2224], 'Alto,': [2225], 'CA)': [2226], 'equipped': [2227], 'diode': [2230], 'array': [2231], 'detector,': [2232], 'A': [2260, 2990, 3911, 4046, 4300, 4458, 4603, 5623], '27-mer': [2261, 4733], '(5′-CCUGCTCAGUACGAGAGGAACCGCAGG-3′),': [2263], 'contains': [2265], 'required': [2267, 5423], 'motif,': [2270, 5426], '(α-sarcin/ricin': [2272], 'loop),': [2273], 'synthesized': [2275], '1-μmol': [2278], 'scale': [2279], 'HPLC-purified': [2281, 3028], 'CyberSyn': [2283, 3030], '(Lenni,': [2284], 'PA).': [2285, 3032], '(oligo)': [2288], '(5': [2289], 'μg)': [2290, 2369], 'labeled': [2292, 2513, 2532, 4537], '5′-end': [2295, 3034, 4834], '[γ-32P]ATP': [2297, 2527], 'T4': [2299], 'polynucleotide': [2300, 2450, 2468], 'kinase': [2301, 2451], 'Biochemicals),': [2304], 'unincorporated': [2307, 2490], 'label': [2308, 2491], 'removed': [2310, 2493], 'Sephadex': [2312, 2495], 'G-25': [2313], 'quick': [2314, 2497], 'spin': [2315, 2498], 'oligo': [2316, 2333, 2347, 2553, 2563, 2755, 2791, 2903, 2913, 2936, 2942, 3019, 3022, 3036, 3058, 3171, 3898, 4033, 4499, 4513, 4540, 4570, 4880, 4912, 4945, 5416, 5437], 'columns': [2317, 2500], 'according': [2321], "manufacturer's": [2324], 'instructions.': [2325, 2507], 'As': [2326, 4296], 'control,': [2328], '32P-labeled': [2332, 2787, 2900, 3890, 4025, 4272, 4496, 4567, 4877, 5117], '(5′-CGCGCUUUUGCGCG-3′)': [2334, 5417], 'does': [2336, 5419], 'contain': [2338, 5421], 'motif': [2342], 'GAGA.': [2343], 'radiolabeled': [2345, 2538, 2549, 2561, 2609, 2745, 2751, 4230, 4842, 5569], 'control': [2346, 2562, 2763, 3178, 3722, 4488, 5054, 5415, 5436], 'prepared': [2349], 'procedures': [2352], 'above': [2354], 'SR': [2357, 2551, 2753, 2789, 2901, 2911, 3896, 4031, 4114, 4497, 4511, 4538, 4568, 4878, 4962, 5031, 5061, 5118], 'oligo.': [2359, 4895, 4964, 5063], '(50': [2368, 4881], 'alkaline-hydrolyzed': [2371], 'treating': [2373, 2777], '500': [2375], 'μl': [2376, 2409, 2441, 2447, 2477, 2583, 2618, 2808, 2918, 3055, 3103, 4519], '(final': [2377], 'volume)': [2378], '100': [2380], 'mm': [2381, 2586, 2591, 2623, 2628, 2631, 2637, 2692, 2699, 3077, 3123, 3128, 3131], 'NaHCO3,': [2382], 'pH': [2383, 2463, 2593, 2639, 2701, 3072, 3125], '9.5,': [2384], 'incubated': [2386, 2644, 2926, 4417, 4491, 4565, 5111], '95': [2388], '°C': [2389, 2577, 2651, 3155], '15': [2391, 2647, 5332], 'min.': [2392, 2659], 'Following': [2393], 'incubation': [2394, 2485, 4534], 'ice': [2396, 2656], 'additional': [2399], '10': [2400, 2446, 2636, 2658, 2698, 3214, 5326], 'min,': [2401], 'reaction': [2403, 2437, 2661, 2813, 2923, 3291, 4440, 4585], 'terminated': [2405], 'adding': [2407], '50': [2408, 2456, 2582, 2897, 3127, 4493], '7.5': [2411], 'm': [2412, 2454, 2461, 3070, 3207], 'ammonium': [2413], 'acetate,': [2414], 'recovered': [2419], 'extraction': [2421], 'phenol/chloroform': [2423], '(1:1)': [2424], 'precipitated': [2426], 'ethanol.': [2428], 'pellet': [2431], 'dissolved': [2433, 3118], 'labeling': [2436], 'mixture': [2438, 2642, 2662, 2814, 2924, 4441, 4586], 'containing': [2439, 4467, 4612], '2.0': [2440], '(100': [2442], 'mCi/ml)': [2443], '[γ-32P]ATP,': [2445], '10×': [2449], 'buffer': [2452, 2621, 2690, 3068, 3110, 3121], '(0.1': [2453, 3069], 'MgCl2,': [2455, 2632], 'mmdithiothreitol,': [2457], '50%': [2458], 'glycerol,': [2459, 2626], '0.5': [2460], 'Tris,': [2462], '9.5),': [2464], '5': [2465, 2579, 3064, 3133, 3141, 5141, 5192, 5344, 5452], 'units': [2466], 'kinase,': [2469], 'volume': [2472, 2805, 4435, 4580], '40': [2476, 4546], 'RNase-free': [2479], 'water.': [2480], 'After': [2481], '30': [2482, 2590, 2650, 2797, 3054, 3102, 4427, 4572, 5124], 'min': [2483, 2580, 2648, 2798, 4428, 4573, 5125], '37': [2487], '°C,': [2488], 'G-50': [2496], 'per': [2504], "supplier's": [2506], 'activities': [2510, 3768, 3807, 3841], '0.73': [2518], '0.9': [2520], 'μCi/μg,': [2521], 'Typically,': [2523, 3083], '45–55%': [2524], 'incorporated': [2529], 'oligoribonucleotide.': [2533], 'For': [2534, 5143], 'saturation': [2535, 4227, 5478], '(3.5': [2543], 'pmol;': [2544, 2555, 2565], '460': [2547, 4279], 'cpm/pmol),': [2548, 2559, 4280, 4847], '(75': [2554, 2564], '140': [2558, 2794, 4502, 4846], 'or': [2560, 2600, 2892, 3175, 4244, 4412, 4486, 4560, 4856, 5282, 5447], '172': [2568], 'cpm/pmol)': [2569, 2795, 4503], 'preincubated': [2571, 2608], 'separate': [2573], 'tubes': [2574], '65': [2576], '350': [2585], 'KCl,': [2587], '20': [2588], 'mmMgCl2,': [2589], 'Tris-HCl,': [2592], '7.5.': [2594], 'Increasing': [2595], 'amounts': [2596, 4262, 4370, 4853], 'mixed': [2605], 'probes,': [2610], 'solution': [2613], '150': [2617, 2807, 2917, 4437, 4518, 4582], '(1': [2622, 2691], 'EDTA,': [2624, 2693, 3078, 3132], '5%': [2625], 'dithiothreitol,': [2629], '0.01%': [2633], 'HEPES,': [2638, 2700, 3071, 3124], '8.0).': [2640], 'another': [2646], 'placed': [2654], 'filtered': [2664, 2816], 'through': [2665, 2817, 2947], 'nitrocellulose': [2667, 2819, 2949, 4446, 4591], '(Millipore': [2669], 'Corp.;': [2670], 'type': [2671], 'HA,': [2672], '0.45-μm': [2673], 'pore': [2674], 'size,': [2675], '25-mm': [2676], 'diameter),': [2677], 'washed': [2682], 'times': [2684, 3258], 'ml': [2687], 'wash': [2689], '0.1%': [2694], 'Tween': [2695], '20,': [2696], '8.0)': [2702], 'time.': [2704], 'dried': [2708, 2954], 'room': [2710, 2800, 2928, 2956, 4430, 4575, 5127], 'temperature': [2711, 2801, 2929, 4431, 4576], 'counted': [2714, 2823], '32P': [2716], 'Beckman': [2719], 'LS6000SC': [2720], 'counter.': [2721], 'background': [2723, 4459, 4604], 'filtration': [2727, 2944, 4464, 4609], 'absence': [2730, 4885], 'subtracted': [2734, 3265, 4474, 4620], 'assay.': [2737, 4477], 'We': [2738, 4073, 4223, 4867, 4949, 5064, 6005], 'examined': [2740, 4869], 'nonspecific': [2742], 'protein.': [2764, 5362, 5947], 'Substrate': [2765], '25': [2778, 3105, 3154], 'pmol': [2779, 2888, 2898, 4270, 4480, 4494, 4547, 4553], 'increasing': [2784, 2905, 4239, 4261, 4408, 4505, 4556, 4852, 4891, 5113, 5136], '(specific': [2792, 4277, 4500, 4844], 'final': [2804, 3138, 4434, 4579], 'buffer.': [2811, 2921, 3193, 4522], 'membrane,': [2820], 'washed,': [2821], 'above.': [2826, 3112], 'plot': [2828], '(22Scatchard': [2829], 'Ann.': [2831], 'Acad.': [2834, 4779, 4798], '1949;': [2836], '51:': [2837], '660-672Crossref': [2838], '(17806)': [2840], 'data': [2847, 3243, 3358, 3907, 4042, 5154], 'performed': [2849, 2884, 5066], '(23Uckun': [2853], 'Gessner': [2855], 'Song': [2857], 'C.W.': [2858], 'Mufson': [2861], 'Blood.': [2863], '1989;': [2864], '73:': [2865], '533-542Crossref': [2866], 'association': [2871, 3277, 3344, 5084, 5392], 'constantKa': [2872], 'slope': [2877, 5297], 'line.': [2880], 'Competition': [2881], 'incubating': [2886], '33': [2887, 4193, 4479], 'BSA': [2893, 4384], '(control)': [2894], '(0–1500': [2907, 4507], 'pmol)': [2908, 4508, 4875, 4882, 5109], 'unlabeled': [2910, 4894, 4944], 'competitor': [2915, 4516], 'h,': [2932], 'unbound': [2935], 'separated': [2938], 'bound': [2941, 4532], 'washing': [2946], 'membrane.': [2950], 'temperature,': [2957], 'radioactivity': [2960], 'before.': [2968], 'Each': [2969, 3922, 4057, 4181, 4439, 4448, 4584, 4593], 'experiment': [2970, 3224], 'repeated': [2972], 'least': [2974, 4455, 4600, 5384], 'two': [2975], 'times,': [2976], 'average': [2979], 'values': [2980, 3331, 3873, 3985, 5288, 5348], 'plotted': [2982], 'CA-CRICKET': [2985], 'Graph': [2986], 'III': [2987], 'application': [2988], 'program.': [2989], 'BIAcore': [2991], '2000': [2992], 'SPR-based': [2993], 'system': [2995, 5159], '(Amersham': [2996], 'Pharmacia': [2997], 'Biotech)': [2998], 'measure': [3002], 'kinetic': [3004, 3145], 'interaction': [3008, 3294, 3507, 4850, 4923], 'soluble': [3010, 3296], '(analytes)': [3014], 'immobilized': [3017, 3040, 3174, 3299], '(ligand).': [3020], '(27-mer)': [3023], 'synthesized,': [3025], '5′-end-biotinylated,': [3026], '(Lennin,': [3031], 'biotinylated': [3035], '(40': [3037], 'μg/ml)': [3038], '(streptavidin-biotin': [3041], 'coupling)': [3042], '(SA)': [3049], 'sensor': [3050, 3165], 'chips': [3051], 'injecting': [3053, 3101], 'flow': [3061, 3158, 3211], 'μl/min': [3065, 3162], 'HBS-EP': [3067, 3109, 3189], '7.4,': [3073], '0.15m': [3074], 'NaCl,': [3075, 3129], '0.005%': [3080], 'polysorbate': [3081], '20).': [3082], 'unit': [3086], 'value': [3087, 3321, 3923, 4058, 4449, 4594], 'increase': [3088], 'following': [3089, 3632], 'procedure': [3091], '70–100.': [3093], 'unoccupied': [3095], 'SA': [3096, 3182], 'blocked': [3099, 3185, 4908, 4941], 'μg/ml': [3106], 'biotin': [3107], 'All': [3113, 5129, 5626], 'HSEM': [3120], '(10': [3122], '8.0,': [3126], 'mmMgCl2)': [3134], 'yield': [3136, 3271, 5953, 5986], 'concentration': [3139, 5172], 'μg/ml.': [3142], 'In': [3143, 4965, 5403, 5727], 'study,': [3146], '30-μl': [3147], '(150': [3149], 'nm)': [3150, 5662, 5680], 'injected': [3152, 3236], '8': [3161], 'chip': [3166], 'had': [3183, 4187, 5612, 5631], 'biotin,': [3187], 'running': [3192], 'Between': [3194], 'samples,': [3195, 3222], 'surfaces': [3198], 'regenerated': [3200], '3-min': [3203], 'injection': [3204, 3256], '2': [3206, 4204, 4221, 5226, 5233, 5240, 5255], 'NaCl': [3208], 'μl/min.': [3215], 'Due': [3216], 'number': [3220], 'carried': [3226], 'out': [3227], 'twice': [3228], 'same': [3231], 'chip,': [3232], 'random': [3238], 'order.': [3239], 'To': [3240], 'prepare': [3241], 'analysis,': [3245], 'lines': [3247], 'zero': [3251], 'curves,': [3254], 'start': [3257], 'aligned.': [3260], 'Background': [3261], 'sensorgrams': [3262, 3269, 3283], 'curves': [3272], 'representing': [3273], 'binding.': [3275, 3382, 5430], 'dissociation': [3279, 3346], 'phases': [3280], 'fit': [3285, 3314], 'simultaneously,': [3286], 'assuming': [3287], 'simple': [3289], 'bimolecular': [3290], 'analyte': [3297], 'ligand,': [3300], 'equivalent': [3301], 'Langmuir': [3304], 'isotherm': [3305], 'adsorption': [3307], 'surface.': [3310], 'goodness': [3312], 'assessed': [3316], 'inspecting': [3318], 'statistical': [3320], 'χ2': [3322, 3330], 'residuals': [3325, 3337], '(observed': [3326], '−': [3327], 'calculated).': [3328], 'low': [3333], '(<2),': [3334], 'randomly': [3339], 'distributed': [3340], 'about': [3341], 'zero.': [3342], 'nonlinear': [3352], 'fitting': [3353, 3905, 4040], 'sensorgram': [3357], 'BIAevaluation': [3361], 'software': [3362], '(version': [3363], '3.0),': [3364], 'supplied': [3365], 'instrument': [3368], '(BIAcore': [3369], 'Inc.).': [3370], 'Affinities': [3371], 'indicated': [3411], 'Asn69,': [3418], 'Asn70,': [3419, 3530], 'Asp92': [3421, 3598], 'Arg122,': [3429], 'involved': [3434], 'rRNA,': [3440, 4977], 'promote': [3441], 'phosphate': [3446, 3650, 3686], 'backbone': [3447, 3546], 'mutations': [3457, 3731, 5277], 'result': [3464], 'destabilization': [3466], 'depicted': [3482], 'Fig.1.': [3484], 'According': [3485], 'model,': [3488], '43,': [3491], '67': [3492], '(paired': [3493], '97),': [3495], '69–70,': [3496], '92,': [3497], '206–210,': [3498], '212–213,': [3499], '217,': [3500], '224–225,': [3501], '253–255': [3503], 'its': [3506, 4849, 4936, 5279, 5548], 'engineered': [3518, 3560], 'Asn69': [3528], 'antiparallel': [3536], 'β': [3537], 'turn': [3538], 'hydrogen': [3542], 'bonds': [3543], 'A+1': [3551], 'position': [3552], 'A+3position.': [3555], '90–92': [3570], '(90FND92),': [3571], 'positioned': [3574], 'β7': [3580], 'strand': [3581], 'RNA.': [3597], 'interacts': [3599, 3644, 3683], 'G+1.': [3604], 'Phe90': [3605, 3619], 'van': [3607], 'der': [3608], 'Waals': [3609], 'four': [3612], 'nearby': [3613], 'hydrophobic': [3614], 'residues.': [3615], 'Thus,': [3616, 5346], 'mutation': [3617], 'anticipated': [3621], 'local': [3625, 4761], 'β8strand': [3629], 'region,': [3634], 'consists': [3636], '121–123': [3639, 3642], '(121SRY123).': [3640], 'sugar,': [3647], 'base,': [3648], 'groups': [3651], 'targeted': [3654, 3689, 3700], 'adenosine.': [3655], 'Arg122': [3679, 3682], 'Tyr123.': [3681], 'adenosine,': [3690], 'Tyr123': [3696], 'stacks': [3697], 'base.': [3702], 'poor': [3710, 3716], 'enzymatic': [3717], 'contrast,': [3720], 'FLP-1': [3725, 3843, 5937], '(28AA29)': [3726], 'FLP-8': [3728, 3845, 5027, 5693, 5939], '(111AA112)': [3729, 5028], 'residues28KD29': [3733], '111SR112that': [3735], 'comparable': [3751, 3847, 4371, 5036, 5961], 'accord': [3761], 'reported': [3765], 'FLP-1,': [3770], 'FLP-4,': [3771, 5317], 'FLP-7,': [3772, 5323], 'FLP-8,': [3773], 'FLP-9,': [3774, 3824, 5329], '(Ala179),': [3776, 4998, 5251], '(Ala208)': [3779, 5259], 'mutants,': [3823, 4982], 'FLP-12,': [3825, 5335], 'FLP-13,': [3827], 'lower': [3830], '(TableI).Table': [3853], 'ISequence': [3854], 'identity,': [3855], 'release,': [3857], 'proteinsMutantOriginal': [3867], 'residuesSubstituted': [3868], 'residuesAdenine': [3869], 'released1-aThe': [3870], 'released': [3872, 3984], 'adopted': [3875, 3987], 'et': [3878, 3990], 'al.': [3879, 3991], '(16)': [3880], 'comparison.Maximum': [3882], 'bound1-bThe': [3883], 'level': [3885, 4020], '(Bmax)': [3888, 4023, 5081, 5200], 'and32P-labeled': [3895, 4030], '(pmol/pmol': [3899, 4034], 'protein)': [3901, 4036], 'Fig.': [3909, 4044, 5014, 5963], '4,': [3910, 4045], 'C,': [3913, 4048, 5045], 'hyperbolic': [3916, 4051], 'function': [3918, 4053], '(Graphpad': [3919, 4054], 'Prism': [3920, 4055], '2).': [3921, 4056, 5964], 'mean': [3926, 4061, 4452, 4597], 'S.D.': [3928, 4063], 'experiments.rRNAOligopmol/μg': [3931], 'RNApmolWild': [3932], 'type396': [3933], '±': [3934, 3936, 3938, 3940, 3942, 3944, 3946, 3948, 3950, 3952, 3954, 3956, 3958, 3960, 3962, 3964, 3966, 3968, 3970, 3972, 3974, 3976, 3978, 3980, 5217, 5225, 5232, 5239, 5246, 5254, 5305, 5313, 5319, 5325, 5331, 5338], '150.79': [3935], '0.031.2': [3937, 3961], '0.2FLP-128KD2928AA29319': [3939], '140.81': [3941], '0.031.3': [3943], '0.2FLP-469NN7069AA70132': [3945], '120.27': [3947], '0.020.53': [3949], '0.1FLP-790FND9290AAA9219': [3951], '50.29': [3953], '0.020.38': [3955], '0.1FLP-8111SR112111AA112376': [3957], '110.75': [3959], '0.1FLP-9122RY123122AA12312': [3963], '30.29': [3965], '0.020.47': [3967], '0.1FLP-12Arg179Ala17914': [3969], '70.28': [3971], '0.020.42': [3973], '0.1FLP-13Trp208Ala2084': [3975], '20.27': [3977], '0.020.48': [3979], '0.11-a': [3981], 'comparison.1-b': [4017], 'experiments.': [4066, 4457, 4602], 'Open': [4067], 'table': [4068], 'new': [4071], 'tab': [4072], 'next': [4074, 4950, 5065, 5575], 'sought': [4075, 5576], 'more': [4077], 'evaluate': [4079], 'regarding': [4086], 'studying': [4099], 'RNAs': [4109], '(23': [4110, 4292], 'oligoribonucleotide).': [4116], 'mutagenesis,': [4128], 'strain,': [4165], 'MV1190,': [4166], 'purified,': [4170], 'SDS-polyacrylamide': [4176], 'electrophoresis': [4178], '(Fig.2': [4179], 'A).': [4180, 4205, 5142], 'apparent': [4189, 5083], 'mass': [4191], 'kDa,': [4194], 'similar': [4195, 5941], '(Fig.': [4203, 4220, 4235, 4752, 4838, 4924, 4946, 5043, 5140, 5191, 5260, 5343, 5451, 5641, 5724], 'immunoreactive': [4215], 'B).': [4222, 4754, 4926, 4948, 5193, 5625, 5643, 5683, 5726], 'first': [4224, 4868, 5517], 'evaluated': [4225], 'A)': [4237], 'independent': [4253], 'experiments': [4254, 5455], 'assays.': [4259, 4866], 'When': [4260], 'reacted': [4267], '3.5': [4269], 'concentration-dependent': [4284, 4915], 'saturable': [4286, 5133], 'rRNA).': [4295], 'evidenced': [4297], 'Fig.4': [4299], 'Table': [4302, 4380], 'I,': [4303, 5013], 'FLP-9(122AA123),': [4308], 'FLP-12(A179),': [4309], 'FLP-13(A208)': [4311], 'exhibited': [4325, 4897, 4931, 5002, 5132, 5940], 'when': [4331, 5994], 'able': [4362], '(Fig.4': [4378], 'A,': [4379, 4407], 'I).': [4381], 'Control': [4382, 4927, 5934], 'did': [4385, 5438], 'to32P-labeled': [4388], 'even': [4393], 'highest': [4396], 'concentration.Figure': [4397], '4RNA': [4398], 'proteins.': [4406, 5104], 'with32P-labeled': [4418], 'μl.': [4438, 4583], 'applied': [4443, 4588], 'filter.': [4447, 4592], '(50–70': [4460, 4605], 'cpm)': [4461, 4606], 'reagents': [4470, 4616], 'except': [4471, 4617], 'B,': [4478], 'protein,': [4489, 5055, 5147, 5311], 'nonlabeled': [4510], 'Binding': [4523], 'percentage': [4528], 'amount': [4531], 'upon': [4533], 'alone.': [4541], '0.2': [4552, 5679], 'BSA.C,': [4555], 'assay.View': [4623], 'Large': [4624], 'Image': [4625], 'Figure': [4626], 'ViewerDownload': [4627], 'Hi-res': [4628], 'image': [4629], 'Download': [4630], '(PPT)': [4631], 'It': [4632], 'known': [4634], 'RNA-PAP': [4728], 'fragment': [4736, 5011, 5034, 5096, 5122, 5208, 5378, 5591, 5715], '(SR': [4745, 5076], 'loop)': [4746], 'Synthetic': [4755], 'fragments': [4757], 'mimic': [4759], 'structures': [4762], 'widely': [4766], 'investigate': [4770], 'RNA-protein': [4771], '(24Munishkin': [4773], 'Wool': [4775], 'I.G.': [4776], 'Proc.': [4777, 4796], 'Natl.': [4778, 4797], 'U.': [4781, 4800], '1997;': [4784], '94:': [4785], '12280-12284Crossref': [4786], '(79)': [4789], '25Ryan': [4792], 'P.C.': [4793], 'Draper': [4794], '88:': [4804], '6308-6312Crossref': [4805], '(35)': [4808], '26Uchiumi': [4811], 'Wada': [4813], 'Kominami': [4815], '270:': [4821], '29889-29893Abstract': [4822], '(23)': [4830], 'C)': [4840], 'with32P': [4843], 'investigated': [4862], 'presence': [4889], 'significant': [4898, 5441], 'oligo,': [4902], 'competitively': [4907, 4940], 'cold': [4911], 'fashion,': [4916], 'confirming': [4917], 'specificity': [4919], '4': [4925, 4947, 5015, 5044, 5218], 'however,': [4930], 'very': [4932], 'little': [4933], 'binding,': [4934], 'naked': [4973], '(90AAA92),': [4987, 5237], '(122AA123),': [4996, 5244], '(Table': [5012], 'C).': [5016, 5345], 'FLP-1(28AA29)': [5025], 'TableI).': [5046], 'non-RNA-binding': [5053], 'no': [5057], 'determine': [5071], 'oligo)': [5078], 'capacity': [5080, 5210, 5270, 5367], 'constant': [5085], '(Ka)': [5086], '(25': [5108], 'temperature.': [5128], 'display': [5149], 'coordinate': [5158], 'yielded': [5160], 'straight': [5162], 'line': [5163], '(r': [5164], '=': [5165, 5649, 5654, 5660, 5678], '−0.99)': [5166], 'over': [5170], '0–22.5': [5175], 'pmol,': [5176], 'supporting': [5177], 'notion': [5179], 'single': [5182], 'class': [5183], 'exists': [5188], 'intercepts': [5195], 'x': [5198], 'axis': [5199], 'correspond': [5202], '21': [5216], 'pmol/μg': [5219, 5227, 5234, 5241, 5248, 5256], '13': [5224, 5231], '(69AA70),': [5230], '14': [5238, 5245, 5320], '12': [5253, 5306], '5,': [5261], 'B': [5262], 'andC).': [5263], 'demonstrate': [5266], 'involving': [5278], 'cleft.': [5285], 'estimated': [5287], 'apparentKa': [5291], 'negative': [5296], 'linear': [5300], 'plots': [5302], '61': [5304], 'pm': [5307, 5315, 5321, 5327, 5333, 5340], '158': [5312], '11': [5314], '148': [5318], '138': [5324], '142': [5330], '132': [5337], '9': [5339], 'theKa': [5347], 'higher': [5355], 'Ka': [5358], 'appears': [5379], 'caused': [5382], 'part': [5386], 'impairment': [5389], 'phase': [5393], 'region.': [5402], 'order': [5404], 'further': [5406, 5456], 'assess': [5407], 'specificity,': [5410], 'a32P-labeled': [5414], 'GAGA,': [5427], 'Filter': [5431], 'either': [5444], 'D).': [5453], 'confirm': [5457], 'specifically': [5461], 'tetraloop.': [5470], 'Taken': [5471], 'together,': [5472], 'competition': [5480], 'confirmed': [5483], 'domains': [5542], 'Because': [5561], 'inherent': [5564, 5978], 'uncertainties': [5565], 'associated': [5566], 'compare': [5578], 'nonradiolabeled': [5587], 'resonance.': [5595], 'much': [5613], 'slower': [5614], 'on-rates': [5615], '(Fig.6,': [5622], 'faster': [5632], 'off-rates,': [5633], 'instability': [5636], '6': [5642, 5725], '(KD': [5648, 5653, 5659, 5677], '1.0': [5650], 'nm),': [5651, 5656], '1.4': [5655], '3.3': [5661], '5-,': [5664], '7-,': [5665], '16-fold': [5667], 'lower,': [5668], 'respectively,': [5669], '(Fig.6': [5682], 'residues111SR112': [5698], 'identical': [5717], 'summary,': [5728], 'Scholar),': [5831], 'Furthermore,': [5948], 'expression,': [5950], 'solubility,': [5951], '(see': [5962], 'Nevertheless,': [5965], 'should': [5969], 'interpreted': [5971], 'caution': [5974], 'because': [5975], 'shortcomings': [5979], 'techniques,': [5983], 'may': [5985], 'nonnative': [5991], 'insoluble': [5999], 'need': [6001], 'renatured.': [6004], 'thank': [6006], 'Tammy': [6007], 'Denton,': [6009], 'Dawn': [6010], 'Dahlke,': [6012], 'Michelle': [6013], 'Henning,': [6015], 'Rebecca': [6016], 'Larue,': [6018], 'Dina': [6019], 'Clementson,': [6020], 'Brad': [6022], 'Nodland': [6023], 'technical': [6025], 'assistance.': [6026]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1995674484', 'counts_by_year': [{'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 1}, {'year': 2019, 'cited_by_count': 1}, {'year': 2018, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 4}, {'year': 2014, 'cited_by_count': 1}], 'updated_date': '2024-12-12T22:17:41.595061', 'created_date': '2016-06-24'}