Title: Foreign Gene Expression in the Mouse Testis by Localizedin VivoGene Transfer
Abstract: In order to attain foreign gene expressionin vivoin the testis of living mice, chloramphenicol acetyltransferase (CAT), firefly luciferase and bacterial lacZ reporter genes were transfected by microparticle bombardment and electroporation. The results showed that CAT reporter gene was expressed in a dose-dependent fashion. The X-gal staining showed that in some spermatogenic-like cells, the bacterial lacZ gene was also expressed byin vivoelectroporation, but not byin vivomicroparticle bombardment. The possibility ofin vivogene transfer to the spermatogenic cells of the mouse testis was further confirmed by the fact that the CAT reporter gene expression was testis-specific when driven by the mouse-protamin 1 promoter. It was concluded, therefore, thatin vivomicroparticle bombardment and, especially, electroporation provide convenient and efficient means of gene transfer to the testis of living mice.
Publication Year: 1997
Publication Date: 1997-04-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 119
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