Title: Determination of carbendazim in water by high-performance immunoaffinity chromatography on-line with high-performance liquid chromatography with diode-array or mass spectrometric detection
Abstract: An automated method for the determination of carbendazim in water that combines high-performance immunoaffinity chromatography (HPIAC), high-performance liquid chromatography (HPLC) in the reversed-phase mode, and detection by either UV-Vis diode array detector (DAD0 spectroscopy or mass spectrometry (MS) is presented here. This method allows for the on-line extraction, preconcentration, and positive confirmation of carbendazim with a throughput of one sample analyzed every 10 min. The method requires minimal manual sample pretreatment, yet it is free from coextracted interferences that often occur in solid-phase extractions based on nonspecific sorbents. The linear range of the calibration curve for 200-μ1 injections of carbendazin is 0.025 to 100 μ/1 for HPIAC-HPLC-MS and 0.075 to 100 μg/1 for HPIAC-HPLC-DAD. Because the calibration curve is mass-dependent, lower detection limits could be achieved by applying larger sample volumes to the HPIAC column. The within-day precision is ±4.5% for HPIAC-HPLC-MS and ±16% for HPIAC-HPLC-DAD for samples containing 0.1 μg/l carbendazim. The results from this method correlate well with results from an ELISA.
Publication Year: 1996
Publication Date: 1996-02-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 25
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