Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W1991829808', 'doi': 'https://doi.org/10.1371/journal.pone.0040467', 'title': 'Testing the Limits of 454 Pyrotag Sequencing: Reproducibility, Quantitative Assessment and Comparison to T-RFLP Fingerprinting of Aquifer Microbes', 'display_name': 'Testing the Limits of 454 Pyrotag Sequencing: Reproducibility, Quantitative Assessment and Comparison to T-RFLP Fingerprinting of Aquifer Microbes', 'publication_year': 2012, 'publication_date': '2012-07-12', 'ids': {'openalex': 'https://openalex.org/W1991829808', 'doi': 'https://doi.org/10.1371/journal.pone.0040467', 'mag': '1991829808', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/22808168', 'pmcid': 'https://www.ncbi.nlm.nih.gov/pmc/articles/3395703'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1371/journal.pone.0040467', 'pdf_url': 'https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0040467&type=printable', 'source': {'id': 'https://openalex.org/S202381698', 'display_name': 'PLoS ONE', 'issn_l': '1932-6203', 'issn': ['1932-6203'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310315706', 'host_organization_name': 'Public Library of Science', 'host_organization_lineage': ['https://openalex.org/P4310315706'], 'host_organization_lineage_names': ['Public Library of Science'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'doaj', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'gold', 'oa_url': 'https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0040467&type=printable', 'any_repository_has_fulltext': True}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5042366928', 'display_name': 'Giovanni Pilloni', 'orcid': 'https://orcid.org/0000-0002-2125-0987'}, 'institutions': [{'id': 'https://openalex.org/I4210127477', 'display_name': 'Institute of Groundwater Ecology', 'ror': 'https://ror.org/03fp59e94', 'country_code': 'DE', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I2802076133', 'https://openalex.org/I4210127477']}, {'id': 'https://openalex.org/I3018134672', 'display_name': 'Helmholtz Zentrum München', 'ror': 'https://ror.org/00cfam450', 'country_code': 'DE', 'type': 'facility', 'lineage': ['https://openalex.org/I1305996414', 'https://openalex.org/I3018134672']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Giovanni Pilloni', 'raw_affiliation_strings': ['Institute of Groundwater Ecology, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany.'], 'affiliations': [{'raw_affiliation_string': 'Institute of Groundwater Ecology, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany.', 'institution_ids': ['https://openalex.org/I4210127477', 'https://openalex.org/I3018134672']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5081690324', 'display_name': 'Michael S. Granitsiotis', 'orcid': 'https://orcid.org/0000-0002-8634-6103'}, 'institutions': [{'id': 'https://openalex.org/I4210127477', 'display_name': 'Institute of Groundwater Ecology', 'ror': 'https://ror.org/03fp59e94', 'country_code': 'DE', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I2802076133', 'https://openalex.org/I4210127477']}, {'id': 'https://openalex.org/I3018134672', 'display_name': 'Helmholtz Zentrum München', 'ror': 'https://ror.org/00cfam450', 'country_code': 'DE', 'type': 'facility', 'lineage': ['https://openalex.org/I1305996414', 'https://openalex.org/I3018134672']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Michael S. Granitsiotis', 'raw_affiliation_strings': ['Institute of Groundwater Ecology, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany.'], 'affiliations': [{'raw_affiliation_string': 'Institute of Groundwater Ecology, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany.', 'institution_ids': ['https://openalex.org/I4210127477', 'https://openalex.org/I3018134672']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5103022016', 'display_name': 'Marion Engel', 'orcid': 'https://orcid.org/0000-0002-0741-8931'}, 'institutions': [{'id': 'https://openalex.org/I3018134672', 'display_name': 'Helmholtz Zentrum München', 'ror': 'https://ror.org/00cfam450', 'country_code': 'DE', 'type': 'facility', 'lineage': ['https://openalex.org/I1305996414', 'https://openalex.org/I3018134672']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Marion Engel', 'raw_affiliation_strings': ['Research Unit Environmental Genomics, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany'], 'affiliations': [{'raw_affiliation_string': 'Research Unit Environmental Genomics, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany', 'institution_ids': ['https://openalex.org/I3018134672']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5065999566', 'display_name': 'Tillmann Lueders', 'orcid': 'https://orcid.org/0000-0002-9361-5009'}, 'institutions': [{'id': 'https://openalex.org/I4210127477', 'display_name': 'Institute of Groundwater Ecology', 'ror': 'https://ror.org/03fp59e94', 'country_code': 'DE', 'type': 'nonprofit', 'lineage': ['https://openalex.org/I2802076133', 'https://openalex.org/I4210127477']}, {'id': 'https://openalex.org/I3018134672', 'display_name': 'Helmholtz Zentrum München', 'ror': 'https://ror.org/00cfam450', 'country_code': 'DE', 'type': 'facility', 'lineage': ['https://openalex.org/I1305996414', 'https://openalex.org/I3018134672']}], 'countries': ['DE'], 'is_corresponding': False, 'raw_author_name': 'Tillmann Lueders', 'raw_affiliation_strings': ['Institute of Groundwater Ecology, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany.'], 'affiliations': [{'raw_affiliation_string': 'Institute of Groundwater Ecology, Helmholtz Zentrum München - German Research Centre for Environmental Health, Neuherberg, Germany.', 'institution_ids': ['https://openalex.org/I4210127477', 'https://openalex.org/I3018134672']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 2, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 1805, 'currency': 'USD', 'value_usd': 1805, 'provenance': 'doaj'}, 'apc_paid': {'value': 1805, 'currency': 'USD', 'value_usd': 1805, 'provenance': 'doaj'}, 'fwci': 17.175, 'has_fulltext': True, 'fulltext_origin': 'pdf', 'cited_by_count': 146, 'citation_normalized_percentile': {'value': 0.999952, 'is_in_top_1_percent': True, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 98, 'max': 99}, 'biblio': {'volume': '7', 'issue': '7', 'first_page': 'e40467', 'last_page': 'e40467'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T12640', 'display_name': 'Environmental DNA in Biodiversity Studies', 'score': 0.9991, 'subfield': {'id': 'https://openalex.org/subfields/2303', 'display_name': 'Ecology'}, 'field': {'id': 'https://openalex.org/fields/23', 'display_name': 'Environmental Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, 'topics': [{'id': 'https://openalex.org/T12640', 'display_name': 'Environmental DNA in Biodiversity Studies', 'score': 0.9991, 'subfield': {'id': 'https://openalex.org/subfields/2303', 'display_name': 'Ecology'}, 'field': {'id': 'https://openalex.org/fields/23', 'display_name': 'Environmental Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T11791', 'display_name': 'Microbial Community Ecology and Physiology', 'score': 0.9989, 'subfield': {'id': 'https://openalex.org/subfields/2303', 'display_name': 'Ecology'}, 'field': {'id': 'https://openalex.org/fields/23', 'display_name': 'Environmental Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T10015', 'display_name': 'Genomics and Phylogenetic Studies', 'score': 0.9941, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/amplicon', 'display_name': 'Amplicon', 'score': 0.67024034}, {'id': 'https://openalex.org/keywords/pyrosequencing', 'display_name': 'Pyrosequencing', 'score': 0.57755303}, {'id': 'https://openalex.org/keywords/barcode', 'display_name': 'Barcode', 'score': 0.5028388}, {'id': 'https://openalex.org/keywords/dna-profiling', 'display_name': 'DNA profiling', 'score': 0.49028042}, {'id': 'https://openalex.org/keywords/environmental-dna', 'display_name': 'Environmental DNA', 'score': 0.4447998}, {'id': 'https://openalex.org/keywords/library', 'display_name': 'Library', 'score': 0.41946656}], 'concepts': [{'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.71902657}, {'id': 'https://openalex.org/C8185291', 'wikidata': 'https://www.wikidata.org/wiki/Q2844457', 'display_name': 'Amplicon', 'level': 4, 'score': 0.67024034}, {'id': 'https://openalex.org/C171636592', 'wikidata': 'https://www.wikidata.org/wiki/Q3395646', 'display_name': 'Terminal restriction fragment length polymorphism', 'level': 5, 'score': 0.6227038}, {'id': 'https://openalex.org/C125009961', 'wikidata': 'https://www.wikidata.org/wiki/Q2342248', 'display_name': 'Pyrosequencing', 'level': 3, 'score': 0.57755303}, {'id': 'https://openalex.org/C70721500', 'wikidata': 'https://www.wikidata.org/wiki/Q177005', 'display_name': 'Computational biology', 'level': 1, 'score': 0.5159536}, {'id': 'https://openalex.org/C2776841711', 'wikidata': 'https://www.wikidata.org/wiki/Q856', 'display_name': 'Barcode', 'level': 2, 'score': 0.5028388}, {'id': 'https://openalex.org/C79386861', 'wikidata': 'https://www.wikidata.org/wiki/Q476697', 'display_name': 'DNA profiling', 'level': 3, 'score': 0.49028042}, {'id': 'https://openalex.org/C15151743', 'wikidata': 'https://www.wikidata.org/wiki/Q903778', 'display_name': 'Metagenomics', 'level': 3, 'score': 0.48443773}, {'id': 'https://openalex.org/C101985253', 'wikidata': 'https://www.wikidata.org/wiki/Q1073526', 'display_name': 'Shotgun sequencing', 'level': 4, 'score': 0.46386877}, {'id': 'https://openalex.org/C168007829', 'wikidata': 'https://www.wikidata.org/wiki/Q857299', 'display_name': 'Restriction fragment length polymorphism', 'level': 4, 'score': 0.44783664}, {'id': 'https://openalex.org/C2779969263', 'wikidata': 'https://www.wikidata.org/wiki/Q25098939', 'display_name': 'Environmental DNA', 'level': 3, 'score': 0.4447998}, {'id': 'https://openalex.org/C51679486', 'wikidata': 'https://www.wikidata.org/wiki/Q380546', 'display_name': 'DNA sequencing', 'level': 3, 'score': 0.4250614}, {'id': 'https://openalex.org/C1436000', 'wikidata': 'https://www.wikidata.org/wiki/Q526826', 'display_name': 'Library', 'level': 4, 'score': 0.41946656}, {'id': 'https://openalex.org/C143121216', 'wikidata': 'https://www.wikidata.org/wiki/Q1330402', 'display_name': 'Microbiome', 'level': 2, 'score': 0.41725364}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.36391398}, {'id': 'https://openalex.org/C42062724', 'wikidata': 'https://www.wikidata.org/wiki/Q1209205', 'display_name': '16S ribosomal RNA', 'level': 3, 'score': 0.32025844}, {'id': 'https://openalex.org/C49105822', 'wikidata': 'https://www.wikidata.org/wiki/Q176996', 'display_name': 'Polymerase chain reaction', 'level': 3, 'score': 0.2935174}, {'id': 'https://openalex.org/C18903297', 'wikidata': 'https://www.wikidata.org/wiki/Q7150', 'display_name': 'Ecology', 'level': 1, 'score': 0.25043666}, {'id': 'https://openalex.org/C552990157', 'wikidata': 'https://www.wikidata.org/wiki/Q7430', 'display_name': 'DNA', 'level': 2, 'score': 0.18501642}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.14528379}, {'id': 'https://openalex.org/C130217890', 'wikidata': 'https://www.wikidata.org/wiki/Q47041', 'display_name': 'Biodiversity', 'level': 2, 'score': 0.0}, {'id': 'https://openalex.org/C41008148', 'wikidata': 'https://www.wikidata.org/wiki/Q21198', 'display_name': 'Computer science', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C111919701', 'wikidata': 'https://www.wikidata.org/wiki/Q9135', 'display_name': 'Operating system', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D001419', 'descriptor_name': 'Bacteria', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D016172', 'descriptor_name': 'DNA Fingerprinting', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D060587', 'descriptor_name': 'Groundwater', 'qualifier_ui': 'Q000382', 'qualifier_name': 'microbiology', 'is_major_topic': True}, {'descriptor_ui': 'D012150', 'descriptor_name': 'Polymorphism, Restriction Fragment Length', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': True}, {'descriptor_ui': 'D017422', 'descriptor_name': 'Sequence Analysis, DNA', 'qualifier_ui': 'Q000379', 'qualifier_name': 'methods', 'is_major_topic': True}, {'descriptor_ui': 'D001419', 'descriptor_name': 'Bacteria', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016172', 'descriptor_name': 'DNA Fingerprinting', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004269', 'descriptor_name': 'DNA, Bacterial', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D015723', 'descriptor_name': 'Gene Library', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005798', 'descriptor_name': 'Genes, Bacterial', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005798', 'descriptor_name': 'Genes, Bacterial', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D014644', 'descriptor_name': 'Genetic Variation', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D060587', 'descriptor_name': 'Groundwater', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012150', 'descriptor_name': 'Polymorphism, Restriction Fragment Length', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012336', 'descriptor_name': 'RNA, Ribosomal, 16S', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D012336', 'descriptor_name': 'RNA, Ribosomal, 16S', 'qualifier_ui': 'Q000235', 'qualifier_name': 'genetics', 'is_major_topic': False}, {'descriptor_ui': 'D015203', 'descriptor_name': 'Reproducibility of Results', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017422', 'descriptor_name': 'Sequence Analysis, DNA', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 5, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1371/journal.pone.0040467', 'pdf_url': 'https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0040467&type=printable', 'source': {'id': 'https://openalex.org/S202381698', 'display_name': 'PLoS ONE', 'issn_l': '1932-6203', 'issn': ['1932-6203'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310315706', 'host_organization_name': 'Public Library of Science', 'host_organization_lineage': ['https://openalex.org/P4310315706'], 'host_organization_lineage_names': ['Public Library of Science'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://doaj.org/article/a8cfcaa1f8554b468b71793dcbb4dcb8', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306401280', 'display_name': 'DOAJ (DOAJ: Directory of Open Access Journals)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': None, 'host_organization_name': None, 'host_organization_lineage': [], 'host_organization_lineage_names': [], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': True, 'landing_page_url': 'https://europepmc.org/articles/pmc3395703', 'pdf_url': 'https://europepmc.org/articles/pmc3395703?pdf=render', 'source': {'id': 'https://openalex.org/S4306400806', 'display_name': 'Europe PMC (PubMed Central)', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1303153112', 'host_organization_name': 'European Bioinformatics Institute', 'host_organization_lineage': ['https://openalex.org/I1303153112'], 'host_organization_lineage_names': ['European Bioinformatics Institute'], 'type': 'repository'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': True, 'landing_page_url': 'https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3395703', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2764455111', 'display_name': 'PubMed Central', 'issn_l': None, 'issn': None, 'is_oa': True, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/22808168', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1371/journal.pone.0040467', 'pdf_url': 'https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0040467&type=printable', 'source': {'id': 'https://openalex.org/S202381698', 'display_name': 'PLoS ONE', 'issn_l': '1932-6203', 'issn': ['1932-6203'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310315706', 'host_organization_name': 'Public Library of Science', 'host_organization_lineage': ['https://openalex.org/P4310315706'], 'host_organization_lineage_names': ['Public Library of Science'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [{'id': 'https://metadata.un.org/sdg/14', 'display_name': 'Life below water', 'score': 0.44}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 24, 'referenced_works': ['https://openalex.org/W1578737958', 'https://openalex.org/W1794545718', 'https://openalex.org/W1922909364', 'https://openalex.org/W1977819146', 'https://openalex.org/W1988925586', 'https://openalex.org/W2007235589', 'https://openalex.org/W2007335873', 'https://openalex.org/W2020541351', 'https://openalex.org/W2031274779', 'https://openalex.org/W2035324108', 'https://openalex.org/W2056857250', 'https://openalex.org/W2098923735', 'https://openalex.org/W2102718644', 'https://openalex.org/W2103412200', 'https://openalex.org/W2114859967', 'https://openalex.org/W2122983968', 'https://openalex.org/W2147717504', 'https://openalex.org/W2152885278', 'https://openalex.org/W2155066423', 'https://openalex.org/W2158877985', 'https://openalex.org/W2163236435', 'https://openalex.org/W2164869506', 'https://openalex.org/W2168104108', 'https://openalex.org/W4254928341'], 'related_works': ['https://openalex.org/W2440089543', 'https://openalex.org/W2380788101', 'https://openalex.org/W2162759181', 'https://openalex.org/W2161558410', 'https://openalex.org/W2088497239', 'https://openalex.org/W2082526065', 'https://openalex.org/W2070790774', 'https://openalex.org/W1980254967', 'https://openalex.org/W1977248415', 'https://openalex.org/W106590866'], 'abstract_inverted_index': {'The': [0, 24, 127], 'characterization': [1], 'of': [2, 21, 26, 54, 62, 72, 82, 92, 109, 129, 140, 158, 168, 191, 210, 218, 227, 248], 'microbial': [3, 259], 'community': [4, 42, 63, 195, 255], 'structure': [5, 256], 'via': [6, 97], '16S': [7, 219], 'rRNA': [8, 220], 'gene': [9, 221], 'profiling': [10], 'has': [11], 'been': [12], 'greatly': [13], 'advanced': [14], 'in': [15, 114, 124, 202], 'recent': [16], 'years': [17], 'by': [18], 'the': [19, 29, 49, 59, 70, 90, 156], 'introduction': [20], 'amplicon': [22, 203], 'pyrosequencing.': [23], 'possibility': [25], 'barcoding': [27], 'gives': [28], 'opportunity': [30], 'to': [31, 58, 102, 166, 216, 231], 'massively': [32], 'screen': [33], 'multiple': [34], 'samples': [35, 112], 'from': [36, 223], 'environmental': [37, 103], 'or': [38], 'clinical': [39], 'sources': [40], 'for': [41, 155], 'details.': [43], 'However,': [44], 'an': [45], 'on-going': [46], 'debate': [47], 'questions': [48], 'reproducibility': [50, 71], 'and': [51, 79, 146, 186, 244, 253], 'semi-quantitative': [52], 'rigour': [53], 'pyrotag': [55, 75, 107, 164, 187, 239], 'sequencing,': [56], 'similar': [57], 'early': [60], 'days': [61], 'fingerprinting.': [64], 'In': [65], 'this': [66], 'study': [67, 235], 'we': [68, 88, 175], 'demonstrate': [69, 176], 'bacterial': [73, 85], '454': [74, 238], 'sequencing': [76, 188, 240], 'over': [77], 'biological': [78], 'technical': [80, 149], 'replicates': [81], 'aquifer': [83, 123], 'sediment': [84, 111, 232], 'communities.': [86, 260], 'Moreover,': [87], 'explore': [89], 'potential': [91], 'recovering': [93, 192, 212, 250], 'specific': [94, 169], 'template': [95, 100, 251], 'ratios': [96, 214], 'quantitatively': [98], 'defined': [99, 162], 'spiking': [101], 'DNA.': [104, 233], 'We': [105], 'sequenced': [106], 'libraries': [108, 206], 'triplicate': [110], 'taken': [113], 'annual': [115], 'sampling': [116], 'campaigns': [117], 'at': [118], 'a': [119, 136, 224, 242], 'tar': [120], 'oil': [121], 'contaminated': [122], 'Düsseldorf,': [125], 'Germany.': [126], 'abundance': [128, 143], 'dominating': [130], 'lineages': [131], 'was': [132, 199], 'highly': [133, 193], 'reproducible': [134, 245], 'with': [135], 'maximal': [137], 'standard': [138], 'deviation': [139], '∼4%': [141], 'read': [142, 159], 'across': [144, 148], 'biological,': [145], '∼2%': [147], 'replicates.': [150], 'Our': [151, 234], 'workflow': [152], 'also': [153, 208], 'allows': [154], 'linking': [157], 'abundances': [160, 252], 'within': [161, 257], 'assembled': [163], 'contigs': [165], 'that': [167, 177, 237], '‘in': [170], 'vivo’': [171], 'fingerprinting': [172], 'signatures.': [173], 'Thus': [174], 'both': [178], 'terminal': [179], 'restriction': [180], 'fragment': [181], 'length': [182], 'polymorphism': [183], '(T-RFLP)': [184], 'analysis': [185], 'are': [189], 'capable': [190, 209, 247], 'comparable': [194], 'structure.': [196], 'Overall': [197], 'diversity': [198], 'roughly': [200], 'double': [201], 'sequencing.': [204], 'Pyrotag': [205], 'were': [207], 'linearly': [211], 'increasing': [213], '(up': [215], '20%)': [217], 'amendments': [222], 'pure': [225], 'culture': [226], 'Aliivibrio': [228], 'fisheri': [229], 'spiked': [230], 'demonstrates': [236], 'is': [241], 'robust': [243], 'method,': [246], 'reliably': [249], 'overall': [254], 'natural': [258]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1991829808', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2023, 'cited_by_count': 1}, {'year': 2022, 'cited_by_count': 2}, {'year': 2021, 'cited_by_count': 2}, {'year': 2020, 'cited_by_count': 7}, {'year': 2019, 'cited_by_count': 9}, {'year': 2018, 'cited_by_count': 18}, {'year': 2017, 'cited_by_count': 11}, {'year': 2016, 'cited_by_count': 22}, {'year': 2015, 'cited_by_count': 23}, {'year': 2014, 'cited_by_count': 28}, {'year': 2013, 'cited_by_count': 16}, {'year': 2012, 'cited_by_count': 5}], 'updated_date': '2024-12-15T13:43:50.391032', 'created_date': '2016-06-24'}