Conservation of Critical Functional Domains in Murine Plasminogen Activator Inhibitor-1

Name: Work Video:
Duration: 3 min 30 s
Zhi Xu; Rashna D. Balsara; Natalia Gorlatova; Daniel A. Lawrence; Francis Castellino; Victoria A. Ploplis
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479, 499, 552], 'is': [3, 266, 278, 541, 662, 673, 714, 768, 828, 1001, 1273, 1599, 1620, 3321, 3975, 4082, 4117], 'the': [4, 46, 107, 116, 127, 185, 193, 199, 215, 228, 254, 263, 279, 321, 382, 391, 402, 460, 468, 474, 490, 503, 529, 538, 666, 674, 722, 775, 832, 883, 891, 921, 1002, 1018, 1133, 1138, 1145, 1209, 1240, 1296, 1302, 1423, 1434, 1471, 1578, 1582, 1602, 1616, 1732, 1873, 1890, 1900, 1936, 1953, 1965, 1968, 2026, 2063, 2087, 2137, 2140, 2268, 2283, 2412, 2425, 2452, 2466, 2505, 2509, 2526, 2535, 2539, 2549, 2553, 2566, 2603, 2631, 2707, 2714, 2729, 2849, 2887, 2897, 2905, 2926, 2930, 2948, 2952, 2960, 2967, 2990, 2995, 3004, 3062, 3093, 3112, 3115, 3131, 3178, 3236, 3258, 3276, 3287, 3291, 3310, 3314, 3324, 3331, 3391, 3437, 3440, 3454, 3494, 3526, 3540, 3552, 3599, 3656, 3659, 3670, 3714, 3718, 3731, 3786, 3792, 3803, 3823, 3838, 3853, 3875, 3891, 3902, 3924, 3945, 3966, 3978, 3985, 3989, 4048, 4053, 4071, 4077, 4104, 4107, 4122, 4189, 4193], 'main': [5, 280], 'physiological': [6, 33, 281, 308, 676], 'regulator': [7, 282, 677], 'of': [8, 41, 106, 201, 235, 244, 283, 316, 381, 476, 510, 519, 665, 678, 708, 724, 835, 886, 896, 920, 960, 996, 1004, 1021, 1050, 1056, 1061, 1068, 1075, 1135, 1144, 1184, 1203, 1208, 1235, 1242, 1255, 1437, 1464, 1514, 1535, 1573, 1586, 1596, 1604, 1608, 1628, 1632, 1731, 1843, 1851, 1943, 2033, 2048, 2073, 2136, 2394, 2441, 2459, 2478, 2511, 2519, 2543, 2559, 2582, 2607, 2683, 2693, 2716, 2728, 2765, 2775, 2823, 2856, 2880, 2915, 2929, 2941, 2978, 2989, 3003, 3077, 3089, 3101, 3111, 3148, 3190, 3195, 3217, 3220, 3235, 3243, 3275, 3282, 3342, 3404, 3417, 3431, 3456, 3472, 3481, 3496, 3506, 3511, 3543, 3608, 3618, 3630, 3639, 3650, 3658, 3664, 3683, 3697, 3702, 3713, 3733, 3744, 3749, 3770, 3795, 3822, 3852, 3868, 3874, 3923, 3969, 3980, 3988, 3996, 4006, 4080, 4125, 4161], 'tissue-type': [9, 284], 'plasminogen': [10, 23, 43, 87, 194, 202, 222, 285, 298, 318, 362, 469, 477, 497, 559, 570, 613, 624, 683, 1447, 3139, 3191], 'in': [12, 21, 29, 31, 51, 81, 262, 287, 296, 304, 306, 326, 356, 537, 721, 783, 831, 926, 1011, 1216, 1228, 1239, 1250, 1301, 1422, 1520, 1560, 1577, 1589, 1625, 1964, 2091, 2145, 2482, 2487, 2508, 2538, 2585, 2640, 2829, 2863, 3012, 3168, 3210, 3250, 3385, 3445, 3675, 3856, 3896, 3949, 4029, 4052, 4142], 'normal': [13, 288], 'plasma.': [14, 289], 'In': [15, 290, 1409, 1462], 'addition': [16, 291, 2705, 2727], 'to': [17, 78, 90, 115, 227, 292, 353, 365, 390, 502, 820, 841, 880, 1017, 1047, 1080, 1377, 1383, 1428, 1600, 1898, 2028, 2099, 2158, 2162, 2190, 2255, 2262, 2266, 2270, 2484, 2502, 2525, 2534, 2579, 2611, 2704, 2706, 2779, 2838, 2886, 3009, 3071, 3074, 3137, 3185, 3229, 3306, 3309, 3323, 3411, 3492, 3524, 3550, 3688, 3729, 3781, 3801, 3815, 3984, 4065, 4098, 4131, 4150], 'its': [18, 82, 91, 293, 357, 366, 1252, 3347, 3952, 4001, 4010, 4092, 4114], 'critical': [19, 294, 1256, 1420], 'function': [20, 295, 845, 3491, 3510, 3728, 3748], 'fibrinolysis,': [22, 297], 'has': [26, 301, 757, 1261, 1374], 'been': [27, 302, 1262, 1307, 1375], 'implicated': [28, 303], 'roles': [30, 305], 'other': [32, 307, 1544, 3292, 3315], 'and': [34, 54, 65, 97, 141, 160, 241, 309, 329, 340, 372, 416, 435, 516, 672, 681, 741, 745, 763, 824, 838, 882, 1193, 1266, 1284, 1584, 1645, 1672, 1688, 1736, 1739, 1976, 1985, 1994, 2003, 2046, 2115, 2161, 2253, 2265, 2327, 2378, 2386, 2392, 2415, 2618, 2649, 2666, 2671, 2758, 2969, 3061, 3069, 3107, 3114, 3122, 3145, 3151, 3154, 3161, 3180, 3198, 3201, 3223, 3253, 3279, 3290, 3297, 3313, 3330, 3346, 3373, 3387, 3408, 3439, 3547, 3568, 3610, 3669, 3798, 3811, 3832, 3913, 3991, 4000, 4009, 4043, 4138, 4192], 'pathophysiological': [35, 310], 'processes.': [36, 311], 'To': [37, 312, 2601], 'investigate': [38, 313], 'structure-function': [39, 314], 'aspects': [40, 315], 'mouse': [42, 317, 1853, 2054, 2278, 2395], 'inhibitor-1,': [45, 320], 'recombinant': [47, 58, 322, 333, 1590, 1711, 2074, 2138, 2319, 3083, 3225], 'protein': [48, 118, 180, 323, 393, 455, 771, 889, 1053, 1154, 1522, 2039, 2367, 3289, 3312, 3326, 3765], 'was': [49, 76, 182, 189, 324, 351, 457, 464, 1474, 1568, 1706, 1715, 1724, 1864, 1907, 1912, 2011, 2023, 2041, 2076, 2156, 2170, 2204, 2310, 2434, 2463, 2480, 2499, 2522, 2545, 2562, 2577, 2609, 2633, 2724, 2747, 2753, 2781, 2836, 2956, 3007, 3042, 3067, 3205, 3238, 3352, 3466, 3500, 3522, 3537, 3620, 3707, 3738, 3789, 3894, 3927, 4012, 4027, 4056, 4173, 4198], 'expressed': [50, 325, 2900, 3239, 3256, 3389], 'Escherichia': [52, 327, 1874], 'coli': [53, 328, 1875, 1922, 2065], 'purified.': [55, 330], 'Five': [56, 331], 'variant': [57, 74, 109, 129, 169, 332, 349, 384, 404, 444, 1430, 2057, 3117, 3357], 'murine': [59, 229, 334, 504, 1579, 1591, 1617, 2058, 3146, 3152, 3160, 3181, 3197, 3222, 3344, 3457, 3580, 3685, 3691, 3704, 3771, 3783, 3817, 3857, 3897, 3970, 3998, 4030, 4085, 4126], 'proteins': [60, 335, 898, 2075, 2227, 2264, 2320, 2348, 2443, 3048, 3134, 4066, 4178], '(R76E,': [61, 336, 3118], 'Q123K,': [62, 337, 3119, 3149, 3277], 'R346A,': [63, 338], 'R101A,': [64, 339, 3120, 3278], 'Q123K/R101A)': [66, 341], 'were': [67, 342, 1648, 1658, 1677, 1692, 1742, 1811, 1820, 1916, 1929, 1950, 2060, 2106, 2129, 2143, 2188, 2228, 2251, 2286, 2294, 2321, 2349, 2362, 2374, 2398, 2444, 2659, 2669, 2695, 2795, 2872, 2893, 2908, 2937, 2964, 2992, 3049, 3056, 3086, 3095, 3128, 3227, 3248, 3284, 3381, 3419, 3433, 3443, 3485, 3596, 3641, 3652, 3673, 3722, 3879, 3905, 4035, 4179, 4186], 'also': [68, 190, 343, 465, 829, 878, 3956], 'generated': [69, 344, 1743, 1951, 2012, 2973], 'using': [70, 345, 1882, 1967, 2014, 2195, 2258, 2451, 2785, 2983, 3207, 3425, 3644], 'site-directed': [71, 346, 1267, 1955], 'mutagenesis.': [72, 347], 'The': [73, 167, 348, 442, 706, 874, 1630, 1666, 1683, 1818, 1904, 1909, 1914, 1927, 2007, 2031, 2103, 2127, 2166, 2184, 2202, 2225, 2317, 2347, 2428, 2438, 2517, 2541, 2557, 2657, 2742, 2751, 2869, 2933, 3033, 3047, 3143, 3214, 3232, 3349, 3477, 3498, 3535, 3735, 3767, 4003, 4177], '(R346A)': [75, 350], 'found': [77, 352], 'be': [79, 259, 354, 534, 818, 1899, 3230, 4148], 'defective': [80, 355], 'inhibitory': [83, 358, 2457, 3034, 3065, 3141, 3157, 3186, 3193, 3218, 3233, 4165, 4196], 'activity': [84, 359, 686, 1526, 2458, 2529, 2537, 2551, 3035, 3066, 3169, 3194, 3204, 3234, 3926, 4166, 4197], 'against': [85, 360, 2333, 2760, 3036, 3909, 4039, 4167], 'tissue': [86, 361, 569, 623, 682], 'relative': [89, 114, 364, 389, 3073, 3215, 3308, 3780, 3814, 4064, 4097, 4130], 'wild-type': [92, 117, 367, 392], 'counterpart.': [93, 368], 'Enzyme-linked': [94, 369], 'immunosorbent': [95, 370, 588, 642], 'assay': [96, 371, 2368, 2469], 'surface': [98, 373, 598, 652, 3657], 'plasmon': [99, 374, 599, 653], 'resonance': [100, 375], 'experiments': [101, 376, 2794], 'demonstrated': [102, 170, 191, 377, 445, 466, 3164, 3302, 3335, 3358, 3375, 3559, 3693, 3774, 3808, 3828, 3885, 3939, 3957, 4019], 'reduced': [103, 378], 'vitronectin-binding': [104, 174, 379, 449], 'affinity': [105, 380, 718, 929, 1386, 1529, 2192], '(Q123K)': [108, 128, 383, 403], '(KD': [110, 119, 385, 394, 3462, 3529], '=': [111, 120, 135, 148, 155, 162, 386, 395, 410, 423, 430, 437, 2101, 3040, 3370, 3463, 3530, 3563, 3572, 3583, 3589, 4171], '1800': [112, 387, 3531], 'nm)': [113, 388, 3465, 3532], '5.4': [121, 396, 3464], 'nm).': [122, 397], 'Kinetic': [123, 398, 2962], 'analyses': [124, 399, 2682, 3110, 3189, 3607], 'indicated': [125, 400, 4190], 'that': [126, 192, 214, 253, 401, 467, 489, 528, 964, 1214, 1245, 1276, 1309, 1431, 1539, 1895, 2954, 3890, 4024, 4067], 'had': [130, 405], 'a': [131, 142, 233, 267, 406, 417, 508, 542, 663, 764, 769, 821, 1057, 1066, 1182, 1221, 1247, 1365, 1419, 1429, 1467, 1521, 1725, 2017, 2304, 2313, 2404, 2475, 2489, 2512, 2580, 2612, 2786, 2820, 2854, 2942, 2984, 3165, 3173, 3211, 3241, 3422, 3488, 3509, 3623, 3628, 3662, 3694, 3726, 3747, 3777, 3829, 3833, 3921, 3940, 4089], 'slower': [132, 407, 3560, 3809, 3830], 'association': [133, 408, 2968, 3478, 3541, 3561, 3719, 3753, 3793, 3810, 3831, 3950, 3959], '(kon': [134, 409, 3562, 3582], '2.92': [136, 411, 3564], '×': [137, 150, 157, 164, 412, 425, 432, 439, 2122, 2176, 3565, 3574, 3585, 3591], '104m-1': [138, 413, 3566], 's-1)': [139, 414, 3567, 3576], 'to,': [140, 415], 'faster': [143, 418, 3569, 3812, 3834], 'dissociation': [144, 419, 2970, 3570, 3813, 3835], 'from,': [145, 420], 'vitronectin': [146, 421, 778], '(koff': [147, 422, 3571], '5.3': [149, 424, 3573], '10-2': [151, 426, 3575], 's-1),': [152, 427], '(wild-type': [153, 428], 'kon': [154, 429, 3499, 3536, 3736, 3787], '1.03': [156, 431, 3584], '106m-1': [158, 433, 3586], 's-1': [159, 434], 'koff': [161, 436, 3553, 3588, 3804], '5.27': [163, 438, 3590], '10-3': [165, 440, 3592], 's-1).': [166, 441, 3593], 'Q123K/R101A': [168, 443, 2008, 3280], 'an': [171, 446, 761, 784, 927, 1411, 1571, 1868, 2191, 2416, 2493, 2843, 3426, 3645], 'even': [172, 447, 3336, 3376], 'lower': [173, 448, 3337, 3377], 'ability.': [175, 450], 'Low': [176, 451], 'density': [177, 207, 452, 482, 579, 633, 1151], 'lipoprotein': [178, 208, 453, 483, 580, 634, 1152], 'receptor-related': [179, 209, 454, 484, 581, 635, 1153], 'binding': [181, 456, 892, 1006, 1074, 2774, 2870, 2927, 2986, 3304, 3441, 3474, 3484, 3518, 3545, 3671, 3687, 3706, 3762, 3773, 3797], 'decreased': [183, 198, 458, 473, 3965], 'for': [184, 270, 459, 545, 894, 930, 1008, 1387, 1530, 1555, 1624, 2038, 2111, 2124, 2178, 2282, 2376, 2436, 2651, 2676, 2697, 2749, 2859, 3082, 3092, 3453, 3598, 3633], '(R76E)': [186, 461], 'variant.': [187, 462], 'It': [188, 463], 'inhibitor-1/vitronectin': [196, 471], 'complex': [197, 216, 472, 491, 998, 1044, 1141, 1146, 2495, 2687, 4090], 'interaction': [200, 475, 1134, 1148, 1287, 1312, 3455, 4055], 'with': [205, 220, 272, 480, 495, 547, 712, 774, 999, 1073, 1137, 1149, 1288, 1433, 1523, 1612, 1867, 1952, 2095, 2208, 2230, 2296, 2303, 2323, 2388, 2496, 2552, 2627, 2635, 2663, 2689, 2883, 2912, 2951, 3135, 3183, 3286, 3363, 3460, 3578, 3837, 3846, 3951, 3960, 4091], 'low': [206, 481, 578, 632, 1150], 'protein.': [210, 246, 485, 521, 3840], 'These': [211, 486, 1532, 3806], 'results': [212, 487, 925, 1519, 1631], 'indicate': [213, 488, 1538], 'interactions': [217, 492, 2688, 3612], 'traditionally': [218, 493], 'associated': [219, 494], 'different': [221, 239, 496, 514, 2976, 3757], 'functions': [225, 237, 500, 512, 1607], 'apply': [226, 501], 'system,': [230, 505, 1618], 'thus': [231, 506], 'showing': [232, 507], 'commonality': [234, 509, 1603], 'subtle': [236, 511, 1606], 'among': [238, 513], 'species': [240, 515], 'evolutionary': [242, 517], 'conservation': [243, 518, 3851], 'this': [245, 248, 520, 523, 887, 997, 1062, 1311, 1517, 1597, 1633, 3930, 4059, 4145], 'Further,': [247, 522, 1364], 'study': [249, 524, 1567], 'provides': [250, 525], 'additional': [251, 526, 1809, 2712], 'evidence': [252, 527], 'human': [255, 530, 1236, 1277, 1292, 1536, 1737, 3144, 3162, 3179, 3199, 3224, 3325, 3601, 3848], 'hemostasis': [256, 531], 'system': [257, 532], 'can': [258, 533, 1045, 1279], 'studied': [260, 535], 'effectively': [261, 536], 'mouse,': [264, 539], 'which': [265, 540, 780, 1013, 1619, 2488, 2922, 3320], 'great': [268, 543], 'asset': [269, 544], 'investigations': [271, 546], 'gene-altered': [273, 548], 'mice.': [274, 549], '(PAI-1)': [553], '1The': [554, 608], 'abbreviations': [555, 609], 'used': [556, 610, 2435, 2748, 3523, 3549, 3800], 'are:': [557, 611], 'PAI-1,': [558, 612, 1237, 1293, 3948], 'inhibitor-1;': [561, 615], 'SERPIN,': [562, 616], 'serine': [563, 617, 667], 'protease': [564, 618, 668, 3953, 4093], 'inhibitor;': [565, 619], 'uPA,': [566, 620], 'urokinase;': [567, 621], 'tPA,': [568, 622, 3955], 'activator;': [571, 625], 'VN,': [572, 626, 881, 1012, 1701], 'vitronectin;': [573, 627], 'uPAR,': [574, 628], 'urokinase': [575, 629, 679, 875], 'receptor;': [576, 630], 'LRP,': [577, 631, 2576, 2690], 'protein;': [582, 636], 'sctPA,': [583, 637, 3037], 'single-chain': [584, 638, 1712, 3208], 'tPA;': [585, 639], 'ELISA,': [586, 640], 'enzyme-linked': [587, 641], 'assay;': [589, 643], 'WT,': [590, 644], 'wild-type;': [591, 645], 'NTA,': [592, 646], 'nickel-nitrilotriacetic': [593, 647, 2196], 'acid;': [594, 648], 'pNA,': [595, 649], 'p-nitroaniline;': [596, 650], 'SPR,': [597, 651], 'resonance;': [600, 654], 'RU,': [601, 655], 'response': [602, 656, 2865], 'units;': [603, 657], 'RCL,': [604, 658], 'reactive': [605, 659, 1424], 'center': [606, 660, 1425], 'loop.': [607, 661], 'member': [664], 'inhibitor': [669, 1525], '(SERPIN)': [670], 'superfamily': [671], 'primary': [675, 1860, 2413, 2730], '(uPA)': [680], '(tPA)': [685], '(1Von': [687], 'Mourik': [688], 'J.A.': [689], 'Lawrence': [690, 1104, 1451, 1486, 1749, 1790], 'D.A.': [691, 935, 1105, 1314, 1390, 1452, 1487, 1750, 1761, 1791], 'Loskutoff': [692, 907, 1090], 'D.J.': [693, 908, 1091, 1116], 'J.': [694, 735, 748, 803, 862, 909, 944, 979, 1034, 1092, 1321, 1344, 1354, 1397, 1488, 1768, 1792], 'Biol.': [695, 804, 863, 911, 945, 980, 1094, 1161, 1322, 1398, 1489, 1769, 1793], 'Chem.': [696, 805, 864, 946, 981, 1323, 1399, 1490, 1770, 1794], '1984;': [697], '259:': [698], '14914-14921Abstract': [699], 'Full': [700, 809, 868, 950, 985, 987, 1327, 1403, 1494, 1496, 1774, 1798, 1800], 'Text': [701, 810, 869, 951, 986, 988, 1328, 1404, 1495, 1497, 1775, 1799, 1801], 'PDF': [702, 811, 870, 952, 989, 1329, 1405, 1498, 1776, 1802], 'PubMed': [703, 812, 871, 953, 990, 1038, 1110, 1127, 1165, 1330, 1359, 1406, 1457, 1499, 1755, 1777, 1803], 'Google': [704, 813, 872, 917, 954, 993, 1041, 1100, 1113, 1130, 1168, 1331, 1362, 1407, 1460, 1502, 1758, 1778, 1806], 'Scholar).': [705, 755, 814, 873, 918, 994, 1042, 1131, 1169, 1363, 1408, 1461, 1503, 1807], 'inactivation': [707], 'these': [709, 897, 1225, 1574, 1605, 3842], 'latter': [710, 1198], 'proteases': [711], 'PAI-1': [713, 756, 767, 782, 816, 844, 1070, 1136, 1217, 1278, 1379, 1515, 1537, 1580, 1609, 1738, 1740, 1845, 1854, 1948, 2051, 2059, 2250, 2279, 2396, 2460, 2468, 2536, 2667, 2717, 2767, 2777, 2881, 3078, 3085, 3226, 3245, 3283, 3294, 3365, 3459, 3483, 3512, 3581, 3602, 3692, 3750, 3758, 3870, 3877, 3898, 3903, 3938, 3971, 3999, 4008, 4033, 4081, 4086, 4099, 4127, 4132], 'mediated': [715], 'through': [716, 1054, 1206, 1264, 1286, 1581, 2848], 'high': [717], 'interactions,': [719, 1466], 'resulting': [720, 1200, 1238, 2862, 2898], 'formation': [723], 'stable': [725], 'serpin-protease': [726], 'complexes': [727], '(2Francis': [728], 'C.W.': [729], 'Marder': [730, 736], 'V.J.': [731, 737], 'Colman': [732], 'R.W.': [733], 'Hirsch': [734], 'Salzman': [738], 'E.W.': [739], 'Hemostasis': [740], 'Thrombosis:': [742], 'Basic': [743], 'Principles': [744], 'Clinical': [746], 'Practice.': [747], 'B.': [749, 1342], 'Lippincott': [750], 'Company,': [751], 'Philadelphia,': [752], 'PA1987:': [753], '358Google': [754], 'two': [758, 3293, 3316], 'major': [759], 'conformations,': [760], 'active': [762, 785, 1069, 1435], 'latent': [765, 822, 3946], 'form.': [766, 3947], 'labile': [770], 'unless': [772, 1654], 'complexed': [773], 'plasma': [776], 'protein,': [777], '(VN),': [779], 'stabilizes': [781], 'form': [786], '(3Declerck': [787, 846], 'P.J.': [788, 847], 'DeMol': [789, 848], 'M.': [790, 849, 1350, 1938], 'Alessi': [791, 850], 'M.C.': [792, 851], 'Baudner': [793, 852], 'S.': [794, 853, 904, 906, 941, 1024, 1087, 1089, 1103, 1318, 1346, 1394, 1450, 1477, 1479, 1748, 1765, 1781, 1783], 'Paques': [795, 854], 'E.P.': [796, 855], 'Preissner': [797, 856], 'K.T.': [798, 857], 'Muller-Berghaus': [799, 858], 'G.': [800, 859, 900, 1083, 1120, 1122], 'Collen': [801, 860], 'D.': [802, 861, 1320, 1396, 1767], '1988;': [806, 865], '263:': [807, 866], '15454-15461Abstract': [808, 867], 'Otherwise,': [815], 'would': [817, 1212], 'converted': [819, 3070], 'conformation': [823], 'become': [825], 'inactivated.': [826], 'VN': [827, 931, 1022, 1081, 1388, 1441, 2574, 2721, 2780, 2826, 2955, 3307, 3412, 3418, 3461, 3619, 3640, 3964, 3990, 4120], 'present': [830, 3895], 'extracellular': [833, 1051], 'matrix': [834, 888, 1052], 'many': [836], 'tissues': [837], 'may': [839, 4147], 'serve': [840], 'selectively': [842], 'localize': [843], 'receptor': [876, 922], '(uPAR)': [877], 'binds': [879, 1440], 'N-terminal': [884, 1019, 1869], 'region': [885, 1297, 1850, 1857], 'contains': [890], 'domain': [893, 1020, 1473, 1518, 3176, 3855, 3893, 4026], 'both': [895], '(4Deng': [899, 1082], 'Curriden': [901, 1084, 1117], 'S.A.': [902, 1085, 1118], 'Wang': [903, 1086], 'Rosenberg': [905, 940, 1088], 'Cell': [910, 1093, 1160], '1996;': [912, 1095, 1107, 1454, 1752], '13:': [913, 1096], '1563-1571Crossref': [914, 1097], 'Scopus': [915, 991, 1039, 1098, 1111, 1128, 1166, 1360, 1458, 1500, 1756, 1804], '(430)': [916, 1099], 'Occupancy': [919], 'by': [923, 1660, 1822, 1931, 1935, 2013, 2043, 2078, 2118, 2172, 2289, 2312, 2330, 2352, 2364, 2380, 2400, 2446, 2465, 2504, 2547, 2565, 2719, 2726, 2735, 2769, 2783, 2876, 2895, 2910, 2947, 2974, 3271, 3299, 3354, 3413, 3468, 3487, 3503, 3613, 3643, 3678, 3709, 3725, 3741, 3881, 3907, 3916, 3929, 4014, 4037, 4046, 4058], 'uPA': [924], 'increased': [928, 4083], '(5Wei': [932], 'Y.': [933], 'Waltz': [934], 'Rao': [936], 'N.': [937, 1336], 'Drummond': [938], 'R.J.': [939], 'Chapman': [942], 'H.A.': [943, 1157], '1994;': [947, 1324, 1400, 1771], '269:': [948, 1325, 1357, 1401, 1772], '32380-32388Abstract': [949], 'Scholar),': [955], 'as': [956, 958, 1065, 1744, 1962, 2016, 2411, 2424, 2816, 2901, 3044, 3240, 3257, 3390, 3508, 3746, 4175], 'well': [957], 'activation': [959, 1055, 1448], 'intracellular': [961], 'signaling': [962, 1174], 'reactions': [963, 2871], 'regulate': [965], 'focal': [966], 'adhesion': [967, 1283], 'turnover': [968], '(6Tang': [969], 'H.': [970], 'Kerins': [971], 'D.M.': [972], 'Hao': [973], 'Q.': [974], 'Inagami': [975], 'T.': [976], 'Vaughan': [977], 'D.E.': [978], '1998;': [982, 1491, 1795], '273:': [983, 1492, 1796], '18268-18272Abstract': [984], '(152)': [992], 'Colocalization': [995], 'integrins': [1000, 1010], 'result': [1003, 1067, 3979], 'RGD-containing': [1005], 'sites': [1007, 1543, 4140], 'αv': [1009], 'are': [1014, 1549, 1553, 1635, 1896, 3255, 3388, 3844, 4141], 'located': [1015], 'adjacent': [1016], '(7Suzuki': [1023], 'Oldberg': [1025], 'A.': [1026], 'Hayman': [1027], 'E.G.': [1028], 'Pierschbacher': [1029], 'M.D.': [1030], 'Ruoslahti': [1031], 'E.': [1032, 1921, 2064], 'EMBO': [1033], '1985;': [1035], '4:': [1036], '2519-2524Crossref': [1037], '(277)': [1040], 'This': [1043, 1170, 1566, 1885, 3974], 'lead': [1046], 'local': [1048], 'dissolution': [1049, 1207], 'proteolytic': [1058], 'cascade.': [1059], 'Regulation': [1060], 'process': [1063, 1171, 3932, 4061, 4073], 'occurs': [1064], 'sterically': [1071], 'interfering': [1072], 'either': [1076, 1823], 'uPAR': [1077], 'or': [1078, 1831, 2056, 2575, 2685, 2722], 'integrin': [1079], 'Scholar,': [1101, 1114, 1332, 1759, 1779], '8Stefansson': [1102], 'Nature.': [1106, 1453, 1751], '383:': [1108, 1455, 1753], '441-443Crossref': [1109, 1456, 1754], '(605)': [1112, 1459, 1757], '9Loskutoff': [1115], 'Hu': [1119], 'Deng': [1121], 'APMIS.': [1123], '1999;': [1124], '107:': [1125], '54-61Crossref': [1126], '(144)': [1129], 'Additionally,': [1132, 3328], 'surface-associated': [1139], 'uPA-uPAR': [1140], 'induces': [1142], 'internalization': [1143], 'via': [1147, 1175], '(LRP)': [1155], '(10Chapman': [1156], 'Curr.': [1158], 'Opin.': [1159], '1997;': [1162], '9:': [1163], '714-724Crossref': [1164], '(421)': [1167], 'alters': [1172], 'cytoplasmic': [1173], 'uPA-uPAR.': [1176], 'Because': [1177, 4136], 'cell': [1178, 1185, 1204, 1282, 2088, 2141, 2168, 2852, 2945], 'proliferation': [1179], 'relies': [1180], 'on': [1181, 1615, 2959, 3130], 'cycle': [1183], 'attachment,': [1186], 'spreading,': [1187], 'flattening': [1188], '(to': [1189], 'initiate': [1190], 'DNA': [1191, 1641, 1932, 2044], 'synthesis),': [1192], 'then': [1194, 2116, 2205, 2328, 2500, 2563, 2660, 2873, 3050, 3434, 3501, 3548, 3653, 3739, 3799, 3914, 4044, 4180], 'rounding': [1195], 'up': [1196], '(the': [1197], 'property': [1199], 'from': [1201, 1650, 1679, 1694, 1708, 1717, 1727, 1813, 2762, 2935, 2939, 2966, 3539, 3791], 'disengagement': [1202], 'contacts': [1205], 'matrix),': [1210], 'it': [1211, 1272], 'seem': [1213], 'alterations': [1215], 'expression': [1218, 1583, 1876, 2040, 2066], 'could': [1219], 'have': [1220, 1231, 1306], 'profound': [1222], 'effect': [1223, 3696, 4146], 'upon': [1224], 'cellular': [1226], 'events.Previous': [1227], 'vitro': [1229], 'studies': [1230, 1627, 2680], 'elaborated': [1232], 'structure/function': [1233], 'relationships': [1234], 'delineation': [1241], 'functional': [1243, 1540, 1558, 1575, 3175], 'domains': [1244, 1260, 1559, 1576], 'play': [1246], 'significant': [1248, 3166], 'role': [1249], 'regulating': [1251], 'pathophysiology.': [1253], 'Identification': [1254], 'sequences': [1257, 1928], 'within': [1258, 1291, 1470, 1516], 'specific': [1259, 1557], 'achieved': [1263], 'structural': [1265], 'mutagenesis': [1268, 1956], 'studies.': [1269, 3327], 'For': [1270, 2134, 2679, 4103], 'example,': [1271], 'now': [1274], 'known': [1275], 'inhibit': [1280, 1446], 'VN-mediated': [1281], 'migration': [1285], 'VN.': [1289, 3689], 'Sequences': [1290], 'specifically': [1294, 1547], 'encompassing': [1295], '110-147,': [1298], 'primarily': [1299], 'localized': [1300], 'α': [1303], 'helix': [1304], 'E,': [1305, 3785], 'identified': [1308, 1475], 'mediate': [1310], '(11Lawrence': [1313, 1389], 'Berkenpas': [1315, 1391, 1762], 'M.B.': [1316, 1392, 1763], 'Palanniappan': [1317, 1393, 1764], 'Ginsburg': [1319, 1395, 1766], '15223-15228Abstract': [1326, 1402, 1773], '12Arroyo': [1333], 'De': [1334], 'Prada': [1335], 'Schroeck': [1337], 'F.': [1338], 'Sinner': [1339], 'E.K.': [1340], 'Muehlenweg': [1341], 'Twellmeyer': [1343], 'Speri': [1345], 'Wilhelm': [1347], 'O.G.': [1348], 'Schmitt': [1349], 'Magdolen': [1351], 'V.': [1352], 'Eur.': [1353], 'Biochem.': [1355], '2002;': [1356], '184-192Crossref': [1358], '(38)': [1361], 'mutation': [1366, 1415, 1508, 1972, 1981, 1990, 1999, 2010, 3333], 'at': [1367, 1416, 1509, 1542, 1570, 2108, 2120, 2131, 2174, 2181, 2568, 2621, 2654, 2673, 2700, 2755, 2797, 2819, 2853, 3038, 3052, 3058, 3098, 3383, 3519, 3627, 3661, 3665, 3756, 3763, 4169, 4182, 4188], 'amino': [1368, 1510, 1893], 'acid': [1369, 1511, 1856, 2197], '123': [1370], '(Q': [1371], '→': [1372, 1413, 1506], 'K)': [1373], 'shown': [1376], 'maintain': [1378], 'serpin': [1380], 'activity,': [1381, 3158], 'but': [1382, 1443, 1527], 'significantly': [1384], 'reduce': [1385, 2159], 'contrast,': [1410], 'R': [1412, 1505], 'A': [1414, 2019, 3450], 'position': [1417, 1512], '346,': [1418], 'residue': [1421], 'loop,': [1426], 'leads': [1427], 'interacts': [1432], 'site': [1436, 1469, 2022], 'target': [1438], 'proteases,': [1439], 'normally,': [1442], 'does': [1444], 'not': [1445, 2924, 2957, 4069, 4111], '(8Stefansson': [1449, 1747], 'terms': [1463], 'LRP': [1465, 1723, 2723, 3961], 'cryptic': [1468], 'heparin-binding': [1472], '(13Stefansson': [1476], 'Muhammad': [1478, 1782], 'Cheng': [1480, 1784], 'X.F.': [1481, 1785], 'Battey': [1482, 1786], 'F.D.': [1483, 1787], 'Strickland': [1484, 1730, 1788], 'D.K.': [1485, 1789], '6358-6366Abstract': [1493, 1797], '(114)': [1501, 1805], 'An': [1504, 4050], 'E': [1507], '76': [1513], 'preserved': [1524], 'diminished': [1528], 'LRP.': [1531], 'targeted': [1533, 1548], 'mutations': [1534, 1588], 'changes': [1541], 'than': [1545, 3776, 3944], 'those': [1546, 2940], 'minor': [1550], 'and,': [1551, 2530], 'therefore,': [1552], 'ideal': [1554], 'delineating': [1556], 'select': [1561], 'biological': [1562], 'processes': [1563], 'involving': [1564, 2681], 'PAI-1.': [1565, 1592, 1629, 2497, 3784, 4031], 'aimed': [1569], 'exploration': [1572], 'characterization': [1585, 3682], 'site-specific': [1587], 'One': [1593], 'practical': [1594], 'value': [1595, 3452, 3495, 3528, 3554, 3701, 3732, 3769, 3788], 'work': [1598], 'evaluate': [1601], 'across': [1610], 'species,': [1611], 'special': [1613], 'emphasis': [1614], 'so': [1621], 'widely': [1622], 'employed': [1623], 'vivo': [1626], 'investigation': [1634], 'described': [1636, 1745, 1963], 'herein.EXPERIMENTAL': [1637], 'PROCEDURESMaterials—Restriction': [1638], 'enzymes,': [1639], 'T4': [1640], 'ligase,': [1642], 'reverse': [1643, 1910, 1977, 1986, 1995, 2004], 'transcriptase,': [1644], 'TaqDNA': [1646], 'polymerase': [1647], 'purchased': [1649, 1678, 1693, 1812], 'Promega': [1651], '(Madison,': [1652], 'WI),': [1653], 'otherwise': [1655], 'specified.': [1656], 'Oligonucleotides': [1657], 'synthesized': [1659], 'MWG': [1661], 'Biotech': [1662], '(High': [1663], 'Point,': [1664], 'NC).': [1665], 'gel': [1667], 'purification,': [1668, 1671], 'PCR': [1669], 'product': [1670], 'plasmid': [1673], 'mini/midi': [1674], 'preparation': [1675], 'kits': [1676], 'Qiagen': [1680], '(Valencia,': [1681], 'CA).': [1682, 1721, 1926], 'chromogenic': [1684, 2554, 3212], 'substrates': [1685, 4094], 'H-d-Ile-Pro-Arg-pNA': [1686], '(S-2288)': [1687], 'pyro': [1689], 'Glu-Phe-Lys-pNA': [1690], '(S-2403)': [1691], 'Chromogenix': [1695], 'AB': [1696], '(West': [1697], 'Chester,': [1698, 2472], 'OH).': [1699, 2473], 'Human': [1700, 1710, 1722], 'purified': [1702, 2288, 2311, 2318, 2442, 3132], 'under': [1703, 2383], 'non-denaturing': [1704], 'conditions,': [1705], 'obtained': [1707, 1716, 3597], 'Promega.': [1709], 'tPA': [1713, 2462, 2479, 2507, 2528, 3209], '(sctPA)': [1714], 'Genentech': [1718], '(San': [1719], 'Francisco,': [1720], 'gift': [1726], 'Dr.': [1728], 'Dudley': [1729], 'American': [1733], 'Red': [1734], 'Cross,': [1735], 'mutants': [1741], 'earlier': [1746], '11Lawrence': [1760], '13Stefansson': [1780], 'All': [1808, 2793, 2988], 'reagents': [1810], 'Fisher': [1814], 'Scientific': [1815], '(Pittsburgh,': [1816], 'PA).': [1817], 'data': [1819, 2934], 'analyzed': [1821, 2782, 2993], 'Origin': [1824], 'version': [1825, 1834, 2999], '7.0': [1826], '(Origin': [1827], 'Lab,': [1828], 'Northampton,': [1829], 'MA)': [1830], 'GraphPad': [1832], 'Prism': [1833], '3.0': [1835, 3000], '(GraphPad': [1836], 'Software': [1837], 'Inc.,': [1838], 'San': [1839], 'Diego,': [1840], 'CA)': [1841, 1961, 2423, 2450], 'software.Construction': [1842], 'Mouse': [1844, 1947, 2766], 'Variant': [1846], 'Expression': [1847, 2072], 'Vectors—The': [1848], 'coding': [1849], 'WT': [1852, 2055, 3084, 3113, 3196, 3221, 3244, 3288, 3311, 3343, 3364, 3407, 3458, 3482, 3579, 3609, 3684, 3690, 3703, 3782, 3816, 3839, 3997, 4007], '(amino': [1855], '24-402,': [1858], 'Swiss-Prot': [1859], 'accession': [1861], 'no.': [1862], 'P22777)': [1863], 'cloned': [1865], 'in-frame': [1866], 'His6': [1870], 'tag': [1871], 'into': [1872, 1918, 2025, 2062, 2086], 'vector': [1877], 'pET15-b': [1878], '(Novagen,': [1879, 2069], 'Madison,': [1880, 2070], 'WI)': [1881], 'Nde-I/BamH-I': [1883], 'sites.': [1884], 'construct': [1886], 'lacks': [1887], 'nucleotides': [1888], 'encoding': [1889], 'first': [1891], '23': [1892], 'acids': [1894], 'thought': [1897], 'secretory': [1901], 'signal': [1902, 2899], 'region.': [1903], 'forward': [1905, 1973, 1982, 1991, 2000], 'primer': [1906, 1911], '5′-GGAATTCCATCATATGTTTACCCCTCCGAGAA-3′.': [1908], '5′-CGCGGATCCTCAAGGCTCCATCAC-3′.': [1913], 'plasmids': [1915], 'transformed': [1917, 2061], 'DH5α': [1919], 'competent': [1920], 'cells': [1923, 2105], '(Invitrogen,': [1924], 'Carlsbad,': [1925], 'verified': [1930], 'sequencing': [1933], 'performed': [1934, 2796, 3129, 3249, 3382], 'W.': [1937], 'Keck': [1939], 'Biotechnology': [1940], 'Center': [1941], '(University': [1942], 'Illinois,': [1944], 'Urbana-Champaign,': [1945], 'IL).': [1946, 2372], 'variants': [1949, 2397, 2668, 2778, 3094, 3237, 3281, 3295, 3317, 3603, 3904, 4011, 4034], 'QuikChange': [1954], 'kit': [1957, 2369, 2845], '(Stratagene,': [1958], 'La': [1959], 'Jolla,': [1960], 'manual,': [1966], 'following': [1969], 'oligonucleotides:': [1970], 'R76E': [1971, 2284], 'primer,': [1974, 1978, 1983, 1987, 1992, 1996, 2001, 2005], '5′-GGCACAGCTCATGCCCTCGAGCAGCTCTCCAAGGAG-3′,': [1975], '5′-GCTCCTTGGAGAGCTGCTCGAGGGCATGAGCTGTGC-3′;': [1979], 'Q123K': [1980, 2015, 3409], '5′-TTCCAGACTATGGTGAAAAAGGTGGACTTCTCAGAG-3′,': [1984], '5′-CCTCTGAGAAGTCCACCTTTTTCACCATAGTCTGGA-3;': [1988], 'R346A': [1989, 4105], '5′-GCCTTTGTCATCTCAGCCGCCATGGCCCCCACGGAG-3′,': [1993], '5′-CTCCGTGGGGGCCATGGCTGAGATGACAAAGG-3′;': [1997], 'R101A': [1998, 3153], '5′-CTTTGTCCAGGCCGATCTAGAGCTGGTCCAGGGCTT-3′,': [2002], '5′-GAAGCCCTGGACCAGCTCTAGATCGGCCTGGACAAA-3′.': [2006], 'double': [2009, 3332], 'template.': [2018], 'new': [2020], 'restriction': [2021], 'incorporated': [2024], 'primers': [2027], 'facilitate': [2029], 'screening.': [2030], 'integrity': [2032], 'all': [2034], 'mutant': [2035], 'clones': [2036], 'selected': [2037], 'confirmed': [2042, 2399], 'sequencing.Expression': [2045], 'Purification': [2047], 'Recombinant': [2049], 'Murine': [2050, 3405, 3516, 3557, 3826, 3869, 4162], 'Proteins—Vectors': [2052], 'containing': [2053, 2249, 3014], 'strain': [2067], 'BL21/DE3': [2068], 'WI).': [2071], 'induced': [2077, 2104], 'adding': [2079], 'isopropyl': [2080], 'thio-b-d-galactoside': [2081], '(final': [2082], 'concentration,': [2083], '1': [2084, 2583, 3021, 3104], 'mm),': [2085], 'suspension': [2089], 'pregrown': [2090], 'Luria-Bertani': [2092], 'medium': [2093], 'supplemented': [2094], '100': [2096, 2605, 3015, 3018], 'μg/ml': [2097, 2584, 2828, 3011, 3617], 'ampicillin': [2098], 'A600': [2100], '0.4-0.5.': [2102], 'grown': [2107], '37': [2109, 3053, 4183], '°C': [2110, 2799], 'another': [2112], '3': [2113, 2808], 'h': [2114, 2653], 'harvested': [2117], 'centrifugation': [2119, 2173], '10,000': [2121], 'g': [2123, 2177], '10': [2125, 2220, 2271, 2297, 2595, 2830], 'min.': [2126, 3635], 'pellets': [2128, 2142], 'stored': [2130], '-70': [2132], '°C.': [2133, 2183, 2623, 3054, 4184], 'purification': [2135, 2393], 'proteins,': [2139], 're-suspended': [2144], 'BugBuster™': [2146], 'reagent': [2147], '(5': [2148], 'ml/gram': [2149], 'pellet': [2150], 'weight)': [2151], '(Novagen).': [2152], 'Benzonase': [2153], '(1': [2154], 'μl/ml)': [2155], 'added': [2157, 2481, 2610, 2670], 'viscosity': [2160], 'remove': [2163], 'nucleic': [2164], 'acids.': [2165], 'insoluble': [2167], 'debris': [2169], 'removed': [2171, 3057, 4187], '16,000': [2175], '20': [2179, 2691, 2824, 3010, 3616, 3667], 'min': [2180, 2699], '4': [2182, 2324, 2622, 3910, 4040], 'soluble': [2185], 'PAI-1-containing': [2186], 'supernatants': [2187], 'applied': [2189], 'chromatography': [2193, 2257], 'column': [2194, 2203], '(NTA)': [2198], 'agarose': [2199], '(Qiagen)': [2200], 'resin.': [2201], 'extensively': [2206], 'washed': [2207, 2661], '0.5': [2209, 2231, 2334], 'm': [2210, 2232, 2325, 2335, 2806, 3911, 4041], 'NaCl,': [2211, 2233, 2336, 2591, 2807, 3017], '50': [2212, 2234, 2337], 'mm': [2213, 2221, 2235, 2243, 2272, 2298, 2338, 2590, 2593, 2596, 2803, 2809, 2831, 2917, 3016, 3019, 3022], 'sodium': [2214, 2236, 2299, 2339], 'phosphate,': [2215, 2237, 2300, 2340], '0.1%': [2216, 2238, 2341, 2645, 3024], 'Tween': [2217, 2239, 2342, 2646], '80': [2218, 2240, 2343, 2647], '(v/v),': [2219, 2241, 2344], 'imidazole,': [2222], 'pH': [2223, 2246, 2275, 2301, 2345, 2599, 2814, 2834, 2919, 3031], '7.0.': [2224, 2247, 2276, 2346], 'adsorbed': [2226], 'eluted': [2229, 2295], '10-150': [2242], 'imidazole': [2244], 'gradient,': [2245], 'Fractions': [2248], 'pooled': [2252], 'subjected': [2254], 'gel-filtration': [2256], 'Sephadex': [2259], 'G-25': [2260], '(Sigma)': [2261, 2291], 'desalt': [2263], 'change': [2267], 'buffer': [2269, 2588, 2642, 2818, 3013], 'phosphate': [2273, 2745, 2832], 'buffer,': [2274, 2630, 2665, 2833], 'Pooled': [2277], 'preparations': [2280], '(except': [2281], 'variant)': [2285], 'further': [2287], 'heparin-agarose': [2290], 'chromatography.': [2292, 2316], 'Proteins': [2293, 2373], '7.0,': [2302], '0-1': [2305], 'M': [2306], 'NaCl': [2307], 'gradient.': [2308], '(R76E)-PAI-1': [2309, 3884], 'second': [2314], 'NTA': [2315], 'denatured': [2322, 3906, 4036], 'guanidinium-HCl': [2326, 3912, 4042], 'refolded': [2329, 3915, 4045], 'extensive': [2331], 'dialysis': [2332, 3908, 4038], 'subsequently': [2350], 'concentrated': [2351], 'Centricon': [2353], 'centrifugal': [2354], 'filter': [2355], 'devices': [2356], '(Millipore,': [2357], 'Bedford,': [2358], 'MA).': [2359], 'Protein': [2360], 'concentrations': [2361, 2879, 2977, 3513], 'determined': [2363, 2445, 2464, 2546, 2564, 2894, 3068, 3206, 3467, 3502, 3538, 3708, 3740, 3790, 3880, 4013, 4199], 'Micro': [2365], 'BCA™': [2366], '(Pierce,': [2370], 'Rockford,': [2371], 'assessed': [2375], 'purity': [2377], 'size': [2379], 'SDS-10%': [2381], 'PAGE': [2382], 'non-reducing': [2384], 'conditions': [2385], 'stained': [2387], 'Coomassie': [2389], 'Brilliant': [2390], 'Blue.Expression': [2391], 'Western': [2401, 3108], 'blotting': [2402, 3109], 'employing': [2403, 2800], 'polyclonal': [2405, 2732], 'rabbit-anti-rat-PAI-1-IgG': [2406], '(American': [2407], 'Diagnostica,': [2408], 'Greenwich,': [2409], 'CT)': [2410], 'antibody': [2414], 'alkaline': [2417, 2736], 'phosphatase-conjugated': [2418, 2737], 'goat-anti-rabbit-IgG': [2419, 2738], '(H+L)': [2420, 2739], '(Bio-Rad,': [2421], 'Hercules,': [2422], 'secondary': [2426, 2740], 'antibody.': [2427, 2741], 'chromogen,': [2429, 2743], '5-bromo-4-chloro-3-indolyl': [2430], 'phosphate/nitro': [2431], 'blue': [2432], 'tetrazolium': [2433], 'detection.': [2437, 2750], 'molecular': [2439], 'weights': [2440], 'ProteinChip': [2447], '(Ciphergen,': [2448], 'Fremont,': [2449], 'Au': [2453], 'chip.Inhibitory': [2454], 'Activity': [2455], 'Assay—The': [2456], 'toward': [2461], 'Coatest™': [2467], '(Chromogenix,': [2470], 'West': [2471], 'Briefly,': [2474], 'fixed': [2476], 'amount': [2477, 2518, 2542, 2715], 'excess': [2483], 'undiluted': [2485], 'plasma,': [2486], 'portion': [2490], 'rapidly': [2491], 'forms': [2492, 3878, 4088], 'inactive': [2494], 'activated': [2501], 'plasmin': [2503, 2520, 2544], 'residual': [2506, 2527], 'presence': [2510], 'stimulator': [2513], '(fibrinogen': [2514], 'degradation': [2515], 'products).': [2516], 'formed': [2521], 'directly': [2523], 'proportional': [2524, 2533], 'hence,': [2531], 'inversely': [2532], 'sample.': [2540], 'measuring': [2548], 'amidolytic': [2550], 'substrate,': [2555, 3954], 'S-2403.': [2556], 'release': [2558], 'p-nitroaniline': [2560], '(pNA)': [2561], 'absorbancy': [2567, 2752], '405': [2569, 2756], 'nm.ELISA': [2570], 'Assays—The': [2571], 'capturing': [2572], 'ligand,': [2573], 'diluted': [2578, 3008], 'concentration': [2581], 'phosphate-buffered': [2586, 2628], 'saline': [2587, 2629], '(137': [2589], '2.7': [2592], 'KCl,': [2594], 'Na2HPO4,2mm': [2597], 'KH2PO4,': [2598], '7.4).': [2600], 'coat': [2602], 'plates,': [2604], 'μl/well': [2606], 'ligand': [2608], 'NuncMaxiSorp': [2613], '96-well': [2614], 'microtiter': [2615], 'plate': [2616, 2632], '(Fisher)': [2617], 'incubated': [2619, 2650, 2672, 2696, 3051, 4181], 'overnight': [2620], 'After': [2624, 2710], 'three': [2625, 2711], 'washes': [2626], 'blocked': [2634], '5%': [2636], 'dried': [2637], 'milk': [2638], 'dissolved': [2639], 'washing': [2641, 2664, 2911], '(phosphate-buffered': [2643], 'saline,': [2644], '(v/v))': [2648], '2': [2652, 2677], 'room': [2655, 2674, 2701], 'temperature.': [2656], 'plates': [2658], '3×': [2662], 'temperature': [2675, 2702], 'h.': [2678], 'PAI-1/tPA': [2684], 'PAI-1/VN': [2686, 3705, 3824], 'nm': [2692, 2757], 'each': [2694], '15': [2698], 'prior': [2703], 'LRP-coated': [2708], 'plates.': [2709], 'washings,': [2713], 'captured': [2718], 'immobilized': [2720, 2888, 2958, 3420, 3642], 'visualized': [2725], 'antibody,': [2731], 'rabbit-anti-rat-PAI-1-IgG,': [2733], 'followed': [2734], 'p-nitrophenyl': [2744], '(Sigma),': [2746], 'measured': [2754], 'normalized': [2759], 'readings': [2761], 'noncoated': [2763], 'wells.Characterization': [2764], 'Variants': [2768], 'Surface': [2770], 'Plasmon': [2771], 'Resonance': [2772], '(SPR)—The': [2773], 'label-free': [2776], 'SPR': [2784, 3614], 'BIAcore™': [2787], '3000': [2788, 3415, 3637], '(BIAcore': [2789, 2846, 3001], 'AB,': [2790], 'Uppsala,': [2791], 'Sweden).': [2792], '25': [2798], 'HBS-EP': [2801], '(10': [2802], 'Hepes,': [2804, 3020], '0.15': [2805], 'EDTA,': [2810, 3023], '0.005%': [2811], 'polysorbate': [2812], '20,': [2813], '7.4)': [2815], 'running': [2817, 2884], 'flow': [2821, 2851, 2944], 'rate': [2822, 2855, 2971], 'μl/min.': [2825], '(20': [2827], '4.0)': [2835], 'coupled': [2837], 'sensor': [2839, 3625], 'chip': [2840, 2889, 2906, 3424, 3626, 3660], 'CM-5': [2841], 'utilizing': [2842, 2994], 'amine-coupling': [2844, 3427, 3646], 'AB)': [2847], 'BIAcore': [2850], '5': [2857, 3631], 'ml/min': [2858], '6': [2860, 3634], 'min,': [2861], '∼3,000': [2864], 'units': [2866], '(RUs)': [2867], 'immobilized.': [2868], 'carried': [2874], 'out': [2875], 'injecting': [2877], 'various': [2878, 3059, 3764], '(diluted': [2882], 'buffer)': [2885], 'surface.': [2890], 'Binding': [2891, 3270, 3410], 'levels': [2892, 3216, 3442, 3672], 'monitoring': [2896], 'RUs.': [2902], 'Between': [2903], 'experiments,': [2904], 'surfaces': [2907], 'regenerated': [2909], '30': [2913], 'μl': [2914], '0.1': [2916, 3026], 'HCl,': [2918], '<': [2920, 3367], '2.0,': [2921], 'did': [2923, 4068, 4110], 'alter': [2925], 'properties': [2928], 'VN-immobilized': [2931], 'chips.': [2932], 'sensorgrams': [2936, 2991], 'subtracted': [2938], 'reference': [2943], 'prepared': [2946], 'same': [2949], 'method,': [2950], 'exception': [2953], 'chip.': [2961], 'constants': [2963, 3821], 'calculated': [2965], 'curves': [2972, 3480, 3546, 3721, 3755], 'applying': [2975], 'analytes': [2979], 'onto': [2980, 3421, 3436, 3622, 3655], 'ligand-immobilized': [2981], 'surfaces,': [2982], '1:1': [2985], 'model.': [2987], 'BioEvaluation': [2996], 'software': [2997], 'package': [2998], 'AB).Measurement': [3002], 'Functional': [3005], 'Half-life—PAI-1': [3006], 'PEG-8000,': [3025], 'mg/ml': [3027], 'bovine': [3028], 'serum': [3029], 'albumin,': [3030], '7.4.': [3032], 't': [3039, 4170], '0,': [3041], 'designated': [3043, 4174], '100%': [3045], 'activity.': [3046, 3142, 3246], 'Aliquots': [3055, 4185], 'times': [3060], 'remaining': [3063, 4194], 'sctPA': [3064, 4168, 4195], 'percent': [3072], 'time': [3075, 3447, 3677], 'zero.RESULTSPurification': [3076], 'Proteins—Typical': [3079], 'final': [3080], 'yields': [3081], '3.8': [3087], 'mg/liter': [3088, 3100], 'culture.': [3090, 3102], 'Yields': [3091], 'slightly': [3096], 'better': [3097], '4.0-4.8': [3099], 'Fig.': [3103], 'represents': [3105], 'SDS-PAGE': [3106], 'five': [3116], 'Q123K/R101A,': [3121], 'R346A)': [3123, 3298], 'proteins.Plasminogen': [3124], 'Activator': [3125], 'Inhibitor': [3126], 'Function—Analyses': [3127], 'rPAI-1': [3133, 3200, 3345, 3406, 3432, 3651, 3686, 4163], 'respect': [3136, 3184], 'their': [3138, 3847], 'homologues': [3147], 'R76E,': [3150], 'R101A/Q123K': [3155], 'retain': [3156], 'whereas': [3159], '(R346A)-PAI-1': [3163], 'reduction': [3167], '(Fig.': [3170, 3318, 3448, 3475, 3514, 3533, 3555, 3604, 3899, 3933, 3962, 3972], '2),': [3171], 'confirming': [3172], 'conserved': [3174, 4028], 'between': [3177], 'molecules': [3182], 'activity.Fig.': [3187], '2Comparative': [3188], 'variants.': [3202, 3348, 4002], 'Inhibitory': [3203], 'assay.': [3213], 'activities': [3219], 'considered': [3228], '100%.': [3231, 4176], 'percentage': [3242], 'Assays': [3247, 3380], 'four': [3251], 'replicates,': [3252], 'values': [3254, 3595, 3807, 3843], 'means': [3259], '±': [3260, 3393], 'S.E.View': [3261, 3394], 'Large': [3262, 3395, 3859, 4153], 'Image': [3263, 3396, 3860, 4154], 'Figure': [3264, 3397, 3861, 4155], 'ViewerDownload': [3265, 3398, 3862, 4156], 'Hi-res': [3266, 3399, 3863, 4157], 'image': [3267, 3400, 3864, 4158], 'Download': [3268, 3401, 3865, 4159], '(PPT)VN': [3269], 'ELISA—The': [3272], 'VN-binding': [3273, 3338, 3350, 3360, 3378, 3854], 'capacity': [3274, 3305, 4005], 'compared': [3285, 3362, 3577, 3836], '(R76E': [3296], 'ELISA.': [3300, 3355], '(Q123K)-PAI-1': [3301, 3558], 'impaired': [3303, 3359, 3886, 4020], '3),': [3319], 'similar': [3322], '(R101A)-PAI-1': [3329], '(R101A/Q123K)-PAI-1': [3334], 'abilities.Fig.': [3339], '3VN-binding': [3340], 'ability': [3341, 3351, 3361, 3943, 3968, 3995, 4079, 4109, 4116, 4124], 'examined': [3353], '(Q123K)PAI-1': [3356, 3827], '(p': [3366], '0.005,': [3368], 'n': [3369], '20).': [3371], '(R101A)PAI-1': [3372], '(Q123K/R101A)PAI-1': [3374], 'abilities.': [3379], 'least': [3384, 3666, 3920], 'triplicate': [3386], 'mean': [3392], '(PPT)Kinetic': [3402], 'Characterization': [3403], 'SPR—Approximately': [3414], 'RUs': [3416, 3638], 'CM5': [3423, 3624], 'method.': [3428, 3647], 'Various': [3429, 3648], 'amounts': [3430, 3649], 'injected': [3435, 3621, 3654], 'surface,': [3438], 'detected': [3444, 3674], 'real': [3446, 3676], '4A).': [3449], 'KD': [3451, 3527, 3700, 3768], 'nonlinear': [3469, 3489, 3710], 'regression': [3470, 3711], 'fitting': [3471, 3505, 3712, 3743], 'steady-state': [3473, 3715], '4B).': [3476], 'progress': [3479, 3720, 3754], 'fit': [3486, 3724], 'single-exponential': [3490, 3727], 'obtain': [3493, 3730], 'kobs.': [3497, 3734], 'linear': [3504, 3742], 'kobs': [3507, 3745], '4C).': [3515], '(Q123K)-PAI-1/VN': [3517, 3544], 'steady': [3520], 'state': [3521], 'determine': [3525], '4D).': [3534], 'phase': [3542, 3794], 'calculate': [3551, 3802], '4E).': [3556], 's-1;': [3587], 'Similar': [3594], 'equivalent': [3600], '4F).Fig.': [3605], '4Kinetic': [3606], '(Q123K)PAI-1/VN': [3611, 3761, 3772, 3796], 'analyses.': [3615], 'speed': [3629, 3663], 'μl/min': [3632], 'Approximately': [3636], 'μl/min,': [3668], 'SPR.': [3679], 'A,': [3680, 4017], 'kinetic': [3681, 3820], 'dose-dependent': [3695], 'binding.': [3698, 3716], 'B,': [3699, 4076], 'C,': [3717, 4119], 'nonlinearly': [3723], 'constant': [3737], 'concentrations.': [3751, 3759, 3766], 'Insert,': [3752], 'D,': [3760], 'more': [3775], '300-fold': [3778], 'difference': [3779], 'value.': [3805], 'rPAI-1.': [3818], 'F,': [3819], 'interaction.': [3825], 'Furthermore,': [3841], 'comparable': [3845], 'counterparts,': [3849], 'indicating': [3850, 3889, 4023], 'PAI-1.View': [3858], '(PPT)LRP-binding': [3866], 'Abilities': [3867], 'Variants—The': [3871, 4164], 'LRP-binding': [3872, 3887, 3892, 3942, 3967, 3992, 4004, 4021, 4025, 4078, 4108, 4123, 4139], 'capacities': [3873], 'altered': [3876], 'ELISA': [3882, 4015], 'experiments.': [3883, 4016], 'ability,': [3888, 4022], '5A).': [3900], 'Also,': [3901, 4032], 'removing': [3917, 4047], 'denaturant.': [3918, 4049], 'At': [3919], 'fraction': [3922], 'PAI-1-binding': [3925], 'enhanced': [3928, 3958], 'unfolding/refolding': [3931, 4060, 4072], '5A,': [3934], 'gray': [3935], 'columns).': [3936, 4075, 4102, 4135], 'Active': [3937], 'higher': [3941], '5B).': [3963], '5C).': [3973], 'most': [3976], 'likely': [3977], 'steric': [3981, 4151], 'hindrance': [3982], 'due': [3983, 4149], 'close': [3986, 4143], 'proximity': [3987], 'sites.Fig.': [3993], '5LRP-binding': [3994], '(R76E)PAI-1': [4018], 'increase': [4051, 4112], 'PAI-1/LRP': [4054], 'observed': [4057], '(gray': [4062, 4095, 4128], 'columns)': [4063, 4096, 4129], 'undergo': [4070], '(black': [4074, 4101, 4134], 'if': [4084], 'initially': [4087], 'alone': [4100, 4133], 'mutant,': [4106], 'because': [4113], 'substrate-binding': [4115], 'impaired.': [4118], 'decreases': [4121], 'VN-': [4137], 'proximity,': [4144], 'hindrance.View': [4152], '(PPT)Stability': [4160], '0': [4172], 'times,': [4191]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1990971755', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2023, 'cited_by_count': 1}, {'year': 2021, 'cited_by_count': 1}, {'year': 2018, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 1}, {'year': 2014, 'cited_by_count': 3}, {'year': 2013, 'cited_by_count': 1}, {'year': 2012, 'cited_by_count': 3}], 'updated_date': '2024-12-10T02:13:47.190683', 'created_date': '2016-06-24'}
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