Title: Evidence that a part of cellular uridine of a tomato (Lycopersicon esculentum) cell suspension culture is located in the vacuoles
Abstract: Cells, protoplasts and isolated vacuoles of a tomato (Lycopersicon esculentum) cell suspension culture were analyzed by high pressure liquid chromatography (HPLC) for the presence of uridine. It was found that the uridine content in 108 cells or protoplasts varied between 70 and 150 nmol for different growth stages. The vacuolar location of a part of cellular uridine was evidenced by its co-migration (i) with α-mannosidase, a soluble vacuolar marker, in the gradient used for the purification of vacuoles and (ii) with α-mannosidase and vacuoles (counted microscopically) during repeated centrifugation of isolated vacuoles. Quantitatively, vacuoles sequestered about 13–35% of the amount of uridine present in protoplasts of different culture age. The possible origin of uridine in the vacuoles is discussed.
Publication Year: 1986
Publication Date: 1986-01-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 15
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