Publication Information

{'id': 'https://openalex.org/W1989744744', 'doi': 'https://doi.org/10.1271/bbb.62.2035', 'title': 'P1-Specificity of Aqualysin I (a Subtilisin-type Serine Protease) from<i>Thermus aquaticus</i>YT-1, using P1-Substituted Derivatives of<i>Streptomyces</i>Subtilisin Inhibitor', 'display_name': 'P1-Specificity of Aqualysin I (a Subtilisin-type Serine Protease) from<i>Thermus aquaticus</i>YT-1, using P1-Substituted Derivatives of<i>Streptomyces</i>Subtilisin Inhibitor', 'publication_year': 1998, 'publication_date': '1998-01-01', 'ids': {'openalex': 'https://openalex.org/W1989744744', 'doi': 'https://doi.org/10.1271/bbb.62.2035', 'mag': '1989744744', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/9882104'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1271/bbb.62.2035', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S94095188', 'display_name': 'Bioscience Biotechnology and Biochemistry', 'issn_l': '0916-8451', 'issn': ['0916-8451', '1347-6947'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310311648', 'host_organization_name': 'Oxford University Press', 'host_organization_lineage': ['https://openalex.org/P4310311647', 'https://openalex.org/P4310311648'], 'host_organization_lineage_names': ['University of Oxford', 'Oxford University Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5109099822', 'display_name': 'Terumichi TANAKA', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I74801974', 'display_name': 'The University of Tokyo', 'ror': 'https://ror.org/057zh3y96', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I74801974']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Terumichi TANAKA', 'raw_affiliation_strings': ['Dept. of Biotechnology, The Univ. of Tokyo'], 'affiliations': [{'raw_affiliation_string': 'Dept. of Biotechnology, The Univ. of Tokyo', 'institution_ids': ['https://openalex.org/I74801974']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5090867811', 'display_name': 'Hiroshi Matsuzawa', 'orcid': 'https://orcid.org/0000-0002-2078-1073'}, 'institutions': [{'id': 'https://openalex.org/I74801974', 'display_name': 'The University of Tokyo', 'ror': 'https://ror.org/057zh3y96', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I74801974']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Hiroshi MATSUZAWA', 'raw_affiliation_strings': ['Dept. of Biotechnology, The Univ. of Tokyo'], 'affiliations': [{'raw_affiliation_string': 'Dept. of Biotechnology, The Univ. of Tokyo', 'institution_ids': ['https://openalex.org/I74801974']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5102140423', 'display_name': 'Shuichi Kojima', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I74801974', 'display_name': 'The University of Tokyo', 'ror': 'https://ror.org/057zh3y96', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I74801974']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Shuichi KOJIMA', 'raw_affiliation_strings': ['Department of Industrial Chemistry, Faculty of Engineering, the University of Tokyo'], 'affiliations': [{'raw_affiliation_string': 'Department of Industrial Chemistry, Faculty of Engineering, the University of Tokyo', 'institution_ids': ['https://openalex.org/I74801974']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5109517829', 'display_name': 'Izumi Kumagai', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I74801974', 'display_name': 'The University of Tokyo', 'ror': 'https://ror.org/057zh3y96', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I74801974']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Izumi KUMAGAI', 'raw_affiliation_strings': ['Department of Industrial Chemistry, Faculty of Engineering, the University of Tokyo'], 'affiliations': [{'raw_affiliation_string': 'Department of Industrial Chemistry, Faculty of Engineering, the University of Tokyo', 'institution_ids': ['https://openalex.org/I74801974']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5109243641', 'display_name': 'Kin‐ichiro Miura', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I74801974', 'display_name': 'The University of Tokyo', 'ror': 'https://ror.org/057zh3y96', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I74801974']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Kin-ichiro MIURA', 'raw_affiliation_strings': ['Department of Industrial Chemistry, Faculty of Engineering, the University of Tokyo'], 'affiliations': [{'raw_affiliation_string': 'Department of Industrial Chemistry, Faculty of Engineering, the University of Tokyo', 'institution_ids': ['https://openalex.org/I74801974']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5011681054', 'display_name': 'Takahisa Ohta', 'orcid': 'https://orcid.org/0000-0003-0092-6543'}, 'institutions': [{'id': 'https://openalex.org/I74801974', 'display_name': 'The University of Tokyo', 'ror': 'https://ror.org/057zh3y96', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I74801974']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Takahisa OHTA', 'raw_affiliation_strings': ['Dept. of Biotechnology, The Univ. of Tokyo'], 'affiliations': [{'raw_affiliation_string': 'Dept. of Biotechnology, The Univ. of Tokyo', 'institution_ids': ['https://openalex.org/I74801974']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 4705, 'currency': 'USD', 'value_usd': 4705, 'provenance': 'doaj'}, 'apc_paid': None, 'fwci': 0.777, 'has_fulltext': False, 'cited_by_count': 8, 'citation_normalized_percentile': {'value': 0.601045, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 76, 'max': 77}, 'biblio': {'volume': '62', 'issue': '10', 'first_page': '2035', 'last_page': '2038'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10252', 'display_name': 'Microbial Natural Products and Biosynthesis', 'score': 0.9974, 'subfield': {'id': 'https://openalex.org/subfields/2736', 'display_name': 'Pharmacology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10252', 'display_name': 'Microbial Natural Products and Biosynthesis', 'score': 0.9974, 'subfield': {'id': 'https://openalex.org/subfields/2736', 'display_name': 'Pharmacology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T11617', 'display_name': 'Enzyme Production and Characterization', 'score': 0.9965, 'subfield': {'id': 'https://openalex.org/subfields/1305', 'display_name': 'Biotechnology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T12505', 'display_name': 'Peptidase Inhibition and Analysis', 'score': 0.9939, 'subfield': {'id': 'https://openalex.org/subfields/2730', 'display_name': 'Oncology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/subtilisin', 'display_name': 'Subtilisin', 'score': 0.9694549}, {'id': 'https://openalex.org/keywords/thermus', 'display_name': 'Thermus', 'score': 0.5984573}], 'concepts': [{'id': 'https://openalex.org/C2779811187', 'wikidata': 'https://www.wikidata.org/wiki/Q423655', 'display_name': 'Subtilisin', 'level': 3, 'score': 0.9694549}, {'id': 'https://openalex.org/C2781440490', 'wikidata': 'https://www.wikidata.org/wiki/Q137165', 'display_name': 'Thermus aquaticus', 'level': 4, 'score': 0.9627168}, {'id': 'https://openalex.org/C2777807008', 'wikidata': 'https://www.wikidata.org/wiki/Q87324999', 'display_name': 'Serine protease', 'level': 4, 'score': 0.8113408}, {'id': 'https://openalex.org/C2776027139', 'wikidata': 'https://www.wikidata.org/wiki/Q7783265', 'display_name': 'Thermus', 'level': 4, 'score': 0.5984573}, {'id': 'https://openalex.org/C2779920670', 'wikidata': 'https://www.wikidata.org/wiki/Q1144013', 'display_name': 'Streptomyces', 'level': 3, 'score': 0.58553356}, {'id': 'https://openalex.org/C2776414213', 'wikidata': 'https://www.wikidata.org/wiki/Q183290', 'display_name': 'Serine', 'level': 3, 'score': 0.5257809}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.5106593}, {'id': 'https://openalex.org/C2776714187', 'wikidata': 'https://www.wikidata.org/wiki/Q212410', 'display_name': 'Protease', 'level': 3, 'score': 0.49421385}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.47070667}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.43712977}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.35094106}, {'id': 'https://openalex.org/C71240020', 'wikidata': 'https://www.wikidata.org/wiki/Q186011', 'display_name': 'Stereochemistry', 'level': 1, 'score': 0.34866852}, {'id': 'https://openalex.org/C27911776', 'wikidata': 'https://www.wikidata.org/wiki/Q834023', 'display_name': 'Thermophile', 'level': 3, 'score': 0.34443873}, {'id': 'https://openalex.org/C523546767', 'wikidata': 'https://www.wikidata.org/wiki/Q10876', 'display_name': 'Bacteria', 'level': 2, 'score': 0.1842399}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.06613445}], 'mesh': [{'descriptor_ui': 'D012697', 'descriptor_name': 'Serine Endopeptidases', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D013824', 'descriptor_name': 'Thermus', 'qualifier_ui': 'Q000201', 'qualifier_name': 'enzymology', 'is_major_topic': True}, {'descriptor_ui': 'D001426', 'descriptor_name': 'Bacterial Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D001426', 'descriptor_name': 'Bacterial Proteins', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D001665', 'descriptor_name': 'Binding Sites', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007700', 'descriptor_name': 'Kinetics', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009842', 'descriptor_name': 'Oligopeptides', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009842', 'descriptor_name': 'Oligopeptides', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D012697', 'descriptor_name': 'Serine Endopeptidases', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015842', 'descriptor_name': 'Serine Proteinase Inhibitors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D015842', 'descriptor_name': 'Serine Proteinase Inhibitors', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D013379', 'descriptor_name': 'Substrate Specificity', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013824', 'descriptor_name': 'Thermus', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1271/bbb.62.2035', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S94095188', 'display_name': 'Bioscience Biotechnology and Biochemistry', 'issn_l': '0916-8451', 'issn': ['0916-8451', '1347-6947'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310311648', 'host_organization_name': 'Oxford University Press', 'host_organization_lineage': ['https://openalex.org/P4310311647', 'https://openalex.org/P4310311648'], 'host_organization_lineage_names': ['University of Oxford', 'Oxford University Press'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/9882104', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [{'score': 0.45, 'display_name': 'Life below water', 'id': 'https://metadata.un.org/sdg/14'}], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 15, 'referenced_works': ['https://openalex.org/W1522104226', 'https://openalex.org/W1765698972', 'https://openalex.org/W1800889405', 'https://openalex.org/W1872778842', 'https://openalex.org/W1900253885', 'https://openalex.org/W1967170873', 'https://openalex.org/W1968416749', 'https://openalex.org/W1999404604', 'https://openalex.org/W2042929474', 'https://openalex.org/W2043855562', 'https://openalex.org/W2053174968', 'https://openalex.org/W2063868681', 'https://openalex.org/W2094275533', 'https://openalex.org/W2169701014', 'https://openalex.org/W2588409883'], 'related_works': ['https://openalex.org/W4298151492', 'https://openalex.org/W30085709', 'https://openalex.org/W2812445', 'https://openalex.org/W2400219580', 'https://openalex.org/W2257843274', 'https://openalex.org/W2082553909', 'https://openalex.org/W2053174968', 'https://openalex.org/W1989744744', 'https://openalex.org/W1788981747', 'https://openalex.org/W1666244608'], 'abstract_inverted_index': {'Aqualysin': [0], 'I': [1], 'is': [2], 'an': [3, 12], 'alkaline': [4], 'serine': [5], 'protease': [6], 'isolated': [7], 'from': [8], 'Thermus': [9], 'aquaticus': [10], 'YT-1,': [11], 'extreme': [13], 'thermophile.': [14], 'We': [15], 'have': [16], 'measured': [17], 'the': [18, 37, 53, 63], 'P1-specificity': [19, 64], 'of': [20, 29, 39, 49, 52, 65], 'aqualysin': [21, 40], 'I,': [22, 41], 'using': [23], 'wild-type': [24], 'and': [25, 47], 'five': [26], 'P1-substituted': [27], 'derivatives': [28], 'Streptomyces': [30], 'subtilisin': [31], 'inhibitor': [32], '(SSI).': [33], 'SSIs': [34], 'efficiently': [35], 'inhibited': [36], 'activity': [38], 'with': [42], 'low': [43], 'substrate': [44], 'specificity.': [45], 'Charge': [46], 'hydrophobicity': [48], 'side': [50], 'chain': [51], 'P1': [54], 'amino': [55], 'acid': [56], 'residue': [57], 'showed': [58], 'no': [59], 'significant': [60], 'effect': [61], 'to': [62], 'this': [66], 'enzyme.': [67]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1989744744', 'counts_by_year': [], 'updated_date': '2024-12-11T00:16:55.122883', 'created_date': '2016-06-24'}