Title: The regulation of the synthesis of arginine catabolizing enzymes during the cell cycle in Saccharomyces cerevisiae
Abstract: In ammonia grown cells of S. cerevisiae we observed that arginase and ornithine transaminase, two enzymes catabolizing arginine and susceptible to coordinate induction by arginine, are synthesized once per cell cycle. However, although these enzymes are members of a common metabolic pathway the synthesis of arginase occurs earlier in the cell cycle than ornithine transaminase. The internal concentration of the arginine pool does not show any significant fluctuation during the cell cycle whether S. cerevisiae is grown on arginine or ammonia. The synthesis of arginase and ornithine transaminase occurs at the same stages in the cell cycle in both media although a higher internal arginine concentration is found in cells growing in this amino acid. Therefore, although arginine regulates the amount of enzymes synthesized, the timing of arginase and ornithine transaminase synthesis appears to be independent of the internal concentration of arginine and the presence or absence of this amino acid in the medium. These results do not support the hypothesis that the regulation of enzyme synthesis during the cell cycle is controlled by oscillatory repression or induction.
Publication Year: 1971
Publication Date: 1971-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 16
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