Title: Seqüenciamento de peptídeos usando espectrometria de massas: um guia prático
Abstract: SPECTROMETRY: A PRACTICAL GUIDE.This paper introduces the basics of peptide mass spectra interpretation applied to proteomics and is directed to chemists, biochemists and biologists.The manuscript presents a well detailed protocol aiming to serve as a first choice guide for understanding peptide sequencing.The tutorial was elaborated based on both a thorough bibliographic revision and the author's experience.In order to prove the applicability of the proposed guide, spectra obtained on different instruments have been successfully interpreted by applying the presented rational.