Title: Macrolide Resistance–associated 23S rRNA Mutation in<i>Mycoplasma genitalium</i>, Japan
Abstract: because we included samples from children hospitalized with or without respiratory disease.BLAST search (www.ncbi.nlm.nih.gov/blast/Blast.cgi)and phylogenetic analysis of amplicons from PCR and nested PCR indicated that samples were positive for HKU1 genotype B (samples BRA169, BRA90, and BRA99) or HKU1 genotype A (samples BRA09, BRA37, and BRA104).The sequences obtained in this study have been deposited in GenBank under accession nos.FJ931534.1 (BRA169), GU904424 (BRA37), GU904427 (BRA104), GU904423 (BRA09), GU904425 (BRA90), and GU904426 (BRA99).This may be the oldest collection of human samples in which HKU1 has been detected.To our knowledge, the oldest previous sample positive for HCoV-HKU1 was detected in children in Finland during 1996-1998, without an exact date specifi ed (7).Retrospective studies also have been conducted in the United States and Greece that showed the HKU1 virus in different countries in Europe and North America before its discovery (8,9).We have confi rmed the circulation of HKU1 coronaviruses in children in Brazil in 1995.