Title: Cytotoxic T Lymphocytes are the Prime Mediators of Suppression of the Mixed Lymphocyte Reaction by Alloactivated Cells
Abstract: An alloactivated secondary mixed lymphocyte reaction (MLR) culture (SMC), which was suppressive in the MLR with autologous responder cells, was studied in more detail. In particular, we investigated whether the suppressive activity of this SMC was mediated by cytotoxic T cells or whether bona fide suppressor cells were involved. The SMC suppressed an MLR when the stimulator cells shared Bw57, Bw60, or Dw 19 with the original stimulator. Separation of the SMC into CD4 + and CD8 + fractions demonstrated that the CD8 + fraction contained suppressive and cytotoxic activity against Bw57 or Bw60 antigens, while the CD4 + fraction contained both activities against the Dw19 specificity. The CD8 + fraction was also suppressive in the reverse MLR, while the CD4 + fraction was not. Limiting dilution analysis demonstrated that all CD8 + ‐suppressive cultures were also cytotoxic, whereas in the CD4 + fraction both cytotoxic and non‐cytotoxic suppressor cultures were found.
Publication Year: 1989
Publication Date: 1989-12-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 4
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot