Title: Inhibition of Ras‐expressing Keratinocytes by De‐epidermized Dermis
Abstract:Cancer cells secrete matrix‐digesting enzymes allowing invasion and migration to other locations. Ras cells secrete similar enzymes, but their targets may be different than basement membranes. Our pre...Cancer cells secrete matrix‐digesting enzymes allowing invasion and migration to other locations. Ras cells secrete similar enzymes, but their targets may be different than basement membranes. Our previous research showed that ras keratinocytes, when plated on a dermal layer, invade prior to basement membrane formation, and then appear to stop. Therefore, it can be determined if the basement membrane is a factor crucial in inhibiting invasion. To test this hypothesis, ras‐overexpressing keratinocytes were plated on de‐epidermized dermis (DED), consisting of a dermal compartment and a basement membrane. Plating on the dermis‐side was used as a basement membrane‐negative control. The tissues were fixed at 1, 7, 14, and 21 days for both frozen and paraffin sections. Invasion of keratinocytes was expected in the dermis‐side samples due to the absence of a basement membrane. Surprisingly, no invasion occurred in either condition until the 21‐day mark, where a correlation between invasion and the degradation of the basement membrane became evident. Epidermal differentiation, previously shown to be randomized in the earlier model, will be assessed to determine whether the environment can instruct cells. Grant Funding Source Research, Creative, and Scholarly Activities GrantRead More
Publication Year: 2009
Publication Date: 2009-04-01
Language: en
Type: article
Indexed In: ['crossref']
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