Publication Information

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936, 944, 992, 1087, 1133, 1267, 1494, 1533, 1691, 1717, 1750, 1851, 1934, 2004, 2019, 2043, 2068, 2263, 2266, 2317, 2345, 2376, 2381, 2396, 2416, 2626, 2639, 2698, 2937, 3018, 3142, 3145, 3174, 3231, 3340, 3349, 3851, 4015, 4018, 4022, 4025, 4072, 4081, 4163, 4213, 4286, 4394, 4460, 4463, 4469, 4485], 'regulating': [18, 263], 'B': [19, 264, 517, 619, 624, 693, 739, 757, 766, 820, 929, 1134, 1672], 'cell': [20, 265, 522, 620, 625, 694, 740, 758, 767, 821, 930, 1135, 1529, 1575, 1721], 'proliferation.': [21, 266], 'To': [22, 267, 1877, 2078, 3654, 3748, 4333], 'better': [23, 268, 2925, 3923, 4059, 4304], 'understand': [24, 269, 1327, 3924, 4305], 'enzymatic': [26, 271, 1144, 1268, 2537, 2650, 2946, 3135, 3670, 3708], 'mechanism': [27, 101, 120, 221, 272, 346, 365, 466, 1141, 1276, 1359, 1376, 2545, 2651, 2782, 3927, 3979, 4046, 4342, 4433], 'kinases,': [33, 278, 1016], 'kinetics': [35, 280], 'BTK': [37, 53, 63, 78, 90, 115, 171, 203, 212, 233, 282, 298, 308, 323, 335, 360, 416, 448, 457, 478, 598, 607, 682, 737, 745, 873, 890, 895, 914, 954, 993, 1005, 1143, 1154, 1279, 1305, 1318, 1331, 1339, 1352, 1361, 1368, 1412, 1465, 1732, 1758, 1766, 1884, 2104, 2142, 2165, 2179, 2181, 2248, 2257, 2259, 2357, 2684, 2691, 2938, 2945, 2991, 3077, 3125, 3132, 3152, 3161, 3168, 3170, 3206, 3208, 3222, 3227, 3252, 3258, 3265, 3300, 3354, 3383, 3395, 3414, 3426, 3432, 3549, 3558, 3572, 3622, 3624, 3647, 3661, 3664, 3679, 3699, 3712, 3719, 3739, 3768, 3772, 3791, 3819, 3843, 3872, 3889, 3891, 3903, 3913, 4089, 4315, 4522], 'substrate': [38, 145, 197, 224, 283, 390, 442, 469, 1147, 1280, 1291, 1306, 1387, 1780, 1872, 2231, 2512, 2588, 2715, 2956, 4004, 4155, 4258, 4265, 4329, 4350], 'phosphorylation': [39, 82, 284, 327, 842, 1148, 1160, 1259, 1281, 1307, 3435, 3590, 3606, 3640, 3685], 'were': [40, 137, 152, 285, 382, 397, 1457, 1482, 1501, 1512, 1531, 1578, 1630, 1680, 1713, 1859, 1943, 1966, 2040, 2112, 2155, 2199, 2306, 2338, 2372, 2414, 2435, 2463, 2483, 2494, 2688, 2720, 2744, 2792, 2810, 3380, 3956, 4337, 4406], 'characterized': [41, 286, 776], 'using': [42, 287, 1475, 1606, 1736, 1790, 2136, 2201, 2238, 2400, 2439, 2795, 2972, 3153, 3555, 4362], 'radioactive': [44, 289], 'enzyme': [45, 290, 1989, 2081, 2132, 2850, 3083, 3110, 3146, 3366, 3405, 3840], 'assay.': [46, 291], 'We': [47, 92, 292, 337, 1365, 3854], 'first': [48, 236, 293, 481, 835, 1299, 1314, 1395, 2315, 2969, 4326, 4354], 'examined': [49, 294, 2751, 2774, 3073, 4340], 'whether': [50, 295, 2976, 3074, 3976, 4317, 4328], 'autophosphorylation': [51, 296, 1332, 1350, 3023, 3130, 3138, 3421, 3550, 3740], 'regulates': [52, 297], 'activity.': [54, 91, 299, 336, 1269, 3147], 'Western': [55, 300, 2249, 2252, 3149, 3194, 3556], 'blotting': [56, 301], 'with': [57, 121, 158, 162, 177, 302, 366, 403, 407, 422, 827, 880, 916, 1459, 1503, 1581, 1632, 1657, 1660, 1670, 1675, 1845, 1947, 1953, 1957, 1963, 2116, 2145, 2158, 2331, 2347, 2683, 2690, 2724, 2927, 2981, 2990, 3002, 3078, 3093, 3365, 3403, 3430, 3563, 3567, 3652, 3704, 3769, 3884], 'phosphospecific': [59, 304, 3156], 'antibody': [60, 305, 3157, 3162, 3615, 3631], 'revealed': [61, 113, 306, 358, 2992, 4050, 4513], 'rapidly': [64, 309, 1340], 'autophosphorylates': [65, 310, 1341], 'at': [66, 204, 311, 449, 1342, 1497, 1506, 1519, 1567, 1592, 1602, 1729, 1898, 2130, 2188, 2283, 2342, 2419, 2465, 2561, 2695, 2716, 2746, 2768, 3139, 3382, 3551, 3611, 3672, 3944, 4246, 4256, 4376, 4380], 'Tyr551': [67, 84, 312, 329, 1162, 1343, 3140, 3155, 3412, 3452, 3552, 3605, 3642, 3687, 3735], 'within': [68, 313, 1163, 1169, 1344, 3447, 3453], 'its': [69, 314, 881, 950, 1131, 3731], 'activation': [70, 315, 839, 912, 994, 1145, 1165, 1346, 3455], 'loop': [71, 316, 1166, 1347, 3456], 'vitro.': [73, 318], 'Examination': [74, 319], 'Y551F': [77, 322, 1733, 2260, 3228, 3259, 3266, 3662, 3665, 3700, 3713, 3720, 3755, 3773, 3820, 3844, 3873, 3892, 3914], 'mutant': [79, 324], 'indicated': [80, 325, 4110], 'causes': [85, 330, 615], '10-fold': [87, 332, 3675, 3910], 'increase': [88, 333, 3134, 3144, 3401, 3730], 'then': [93, 338, 871, 1579, 1600, 1668, 1907, 1951, 1962, 2031, 2339, 2495, 2522, 2721, 2773], 'proceeded': [94, 339], 'to': [95, 140, 155, 170, 340, 385, 400, 415, 770, 863, 874, 887, 897, 949, 1083, 1151, 1265, 1316, 1326, 1484, 1489, 1619, 1684, 1722, 1764, 1842, 1887, 1915, 1968, 2070, 2148, 2193, 2206, 2308, 2424, 2429, 2444, 2485, 2497, 2503, 2525, 2599, 2662, 2702, 2706, 2728, 2754, 2775, 2799, 2813, 2847, 2865, 2903, 2943, 3081, 3113, 3193, 3278, 3400, 3418, 3441, 3629, 3668, 3690, 3724, 3848, 3886, 3962, 3974, 3995, 4034, 4103, 4138, 4187, 4303, 4314, 4324, 4391, 4408, 4430, 4445, 4520], 'characterize': [96, 341, 3750], 'steady': [98, 343, 1356], 'state': [99, 344, 1357, 3696], 'kinetic': [100, 220, 238, 345, 465, 483, 1285, 1301, 1358, 1375, 1397, 2816, 2941, 3935, 4266], 'BTK.': [103, 348, 1463, 1757, 2578, 2614, 3698], 'Varying': [104, 349, 4509], 'concentrations': [106, 205, 351, 450, 1858, 2563, 2719, 2771, 3384, 3946, 4247, 4378], 'ATP': [108, 159, 167, 178, 190, 194, 353, 404, 412, 423, 435, 439, 1385, 2067, 2120, 2161, 2177, 2184, 2277, 2411, 2469, 2611, 2886, 2890, 3185, 3764, 3782, 3809, 3852, 3887, 3989, 4085, 4123, 4199, 4311, 4319, 4359, 4371, 4450, 4481, 4504], 'and': [109, 128, 150, 160, 168, 189, 354, 373, 395, 405, 413, 434, 519, 622, 911, 921, 981, 1023, 1146, 1167, 1287, 1321, 1348, 1377, 1386, 1404, 1454, 1511, 1611, 1682, 1715, 1727, 1745, 1748, 1867, 1961, 1970, 2049, 2061, 2097, 2121, 2124, 2162, 2180, 2227, 2352, 2398, 2412, 2427, 2437, 2490, 2507, 2551, 2558, 2568, 2633, 2643, 2733, 2750, 2838, 2858, 2887, 2921, 2965, 3009, 3128, 3159, 3377, 3389, 3451, 3579, 3787, 3796, 3817, 3825, 3871, 3890, 3952, 3988, 3993, 4021, 4084, 4168, 4179, 4204, 4218, 4229, 4312, 4451, 4466, 4482, 4491], 'S1': [110, 169, 188, 355, 414, 433, 1870, 2122, 2413, 2557, 2575, 2955, 2964, 3216, 3861, 3950, 3968, 4083, 4115, 4202, 4243, 4313, 4321, 4373, 4483, 4511], 'peptide': [111, 356, 1319, 1388, 1783, 1871, 2957, 3217, 3862, 3951], '(biotin-Aca-AAAEEIY-GEI-NH2)': [112, 357], 'employs': [116, 361], 'ternary': [118, 363, 1373, 2530, 2543, 2628, 3983, 4036, 4053, 4105], 'complex': [119, 364, 2531, 2544, 2629, 2856, 3984, 4037, 4054, 4106, 4532], 'KmATP': [122, 367], '=': [123, 130, 368, 375, 3256, 3263, 3269, 4524, 4534], '84': [124, 369, 4126, 4132], '±': [125, 132, 370, 377, 3307, 3320, 3323, 3325, 3794, 3798, 3823, 3827, 3905, 3916, 4118, 4127, 4133, 4190, 4268, 4270, 4272, 4274, 4276, 4278, 4280, 4282, 4500, 4526, 4536], '20': [126, 371, 1549, 1652, 1665, 1826, 4128], 'μm': [127, 372, 1824, 1865, 1869, 2160, 2276, 2426, 3184, 3243, 4251, 4502], 'KmS1': [129, 374, 2550], '37': [131, 376, 4117], '8': [133, 378, 1819, 4119], 'μm.': [134, 379, 2195, 4129], 'Inhibition': [135, 380, 2680], 'studies': [136, 381, 4336], 'also': [138, 184, 383, 429, 1018, 1363, 3855], 'performed': [139, 384, 1458, 2018, 2237, 3932], 'examine': [141, 386, 3996], 'order': [143, 388, 1289, 4307], 'binding.': [146, 391, 1292, 3853], 'The': [147, 392, 595, 733, 760, 986, 1355, 1464, 1782, 1793, 1894, 1904, 1979, 1988, 1999, 2128, 2196, 2286, 2312, 2433, 2514, 2539, 2645, 2657, 2761, 2907, 2968, 3245, 3392, 3614, 3896, 4007, 4099, 4144, 4353], 'inhibitors': [148, 199, 393, 444, 1410, 2687], 'ADP': [149, 394, 2888], 'staurosporine': [151, 396, 2884, 4366, 4386, 4442, 4497, 4515], 'both': [153, 398, 618, 3375, 3948], 'found': [154, 185, 399, 430, 1366, 2894, 3017, 3112, 3399, 3667, 3779, 4186, 4444], 'be': [156, 401, 1152, 1406, 2486, 2617, 2637, 3114, 3442, 4188, 4240], 'competitive': [157, 402, 2819, 2897, 2916, 4412, 4447], 'non-competitive': [161, 406], 'S1,': [163, 408, 2471, 3987], 'indicating': [164, 409, 3602], 'binding': [165, 179, 191, 410, 424, 436, 1389, 2846, 4005, 4200, 4203, 4309, 4330, 4360], 'either': [173, 418, 2267, 2353, 2385, 2528, 2814, 2848, 3175, 3187, 3230, 4318, 4370], 'random': [174, 419], 'or': [175, 420, 2258, 2271, 2280, 2363, 2387, 2461, 2470, 2534, 2826, 2853, 2917, 3179, 3189, 3207, 3226, 3237, 4320, 4327, 4347, 4372, 4421], 'ordered': [176, 421], 'first.': [180, 425], 'Negative': [181, 426], 'cooperativity': [182, 427], 'was': [183, 428, 1313, 1324, 1336, 1362, 1467, 1599, 1614, 1617, 1734, 1746, 1784, 1797, 1840, 1885, 1896, 1906, 1977, 1981, 1991, 2007, 2030, 2085, 2107, 2134, 2143, 2186, 2236, 2261, 2288, 2314, 2480, 2521, 2667, 2772, 2868, 2893, 2901, 2948, 2975, 2979, 2996, 3072, 3097, 3111, 3172, 3347, 3356, 3398, 3541, 3553, 3592, 3666, 3702, 3778, 3863, 3909, 3942, 4065, 4116, 4125, 4131, 4185, 4244, 4254, 4322, 4331, 4374, 4387, 4443], 'between': [186, 431, 1383, 2587, 4001, 4154], 'sites.': [192, 437, 1390, 4006], 'Unlike': [193, 438], 'site': [195, 440, 3741, 4361], 'inhibitors,': [196, 441, 2685], 'analog': [198, 443, 4351], 'did': [200, 445, 2911, 3573, 3626], 'not': [201, 446, 996, 2912, 2997, 3574, 3609, 3627, 3722, 4087], 'inhibit': [202, 447], 'less': [206, 451], 'than': [207, 228, 452, 473, 1664, 3594, 3677, 3912, 4249, 4529], '1': [208, 453, 1544, 1560, 1649, 1829, 1860, 2279, 2335, 2379, 3188, 3561, 3578, 3612], 'mm,': [209, 454], 'suggesting': [210, 455, 3733], 'may': [213, 458, 1405], 'employ': [214, 459], '"substrate': [216, 461], 'clamping"': [217, 462], 'type': [218, 463, 1756, 3767, 3790, 3902], 'whereby': [222, 467], 'Kd': [225, 470], 'weaker': [227, 472], 'Km.': [229, 474, 3810], 'This': [230, 475, 838, 2028, 4192, 4473, 4541], 'investigation': [231, 476], 'provides': [234, 479], 'detailed': [237, 482, 1396], 'characterization': [239, 484, 1302, 3352], 'kinase.': [244, 489], '2The': [494], 'abbreviations': [495], 'used': [496], 'are:': [497], 'BTK,': [498, 1526, 3758, 3868, 3870, 3929, 3986], 'kinase;': [501], 'SH3,': [502], 'Src': [503, 823, 971, 977], 'homology': [504, 883, 952, 968, 972, 978], '3.': [505], 'plays': [512], 'an': [513, 1718, 1762, 2016, 2051, 2730, 2777, 3143, 3357], 'essential': [514], 'role': [515, 987, 1086, 1132], 'cells': [518, 1481], 'other': [520, 851, 922, 957, 990, 2566], 'hematopoietic': [521], 'types': [523], '(1Desiderio': [524], 'S.': [525, 642, 660, 705, 1202, 3490], 'Curr.': [526, 1033, 1420, 1441], 'Opin.': [527, 1034], 'Immunol.': [528, 544, 557, 585, 791, 1035, 1121], '1997;': [529, 1180, 3063, 3468], '9:': [530], '534-540Crossref': [531], 'PubMed': [532, 548, 562, 590, 638, 676, 728, 795, 811, 1039, 1060, 1108, 1125, 1188, 1215, 1247, 1426, 1447, 3043, 3066, 3476, 3503, 3535], 'Scopus': [533, 549, 591, 677, 729, 796, 812, 1040, 1061, 1126, 1189, 1216, 1248, 1427, 1448, 3044, 3067, 3477, 3504, 3536], '(69)': [534], 'Google': [535, 551, 563, 593, 639, 679, 731, 798, 814, 1042, 1063, 1109, 1128, 1191, 1218, 1250, 1429, 1450, 3046, 3069, 3479, 3506, 3538], 'Scholar,': [536, 552, 564, 640, 799, 1043, 1110, 1192, 1219, 1430, 3047, 3480, 3507, 4172, 4222], '2Satterthwaite': [537], 'A.B.': [538, 554], 'Li': [539], 'Z.': [540], 'Witte': [541, 555, 667, 722, 1207, 1236, 3495, 3524], 'O.N.': [542, 556, 668, 723, 1208, 1237, 3496, 3525], 'Semin.': [543], '1998;': [545], '10:': [546, 1424], '309-316Crossref': [547], '(159)': [550, 3045], '3Satterthwaite': [553], 'Rev.': [558, 586, 1120], '2000;': [559, 635], '175:': [560], '120-127Crossref': [561], '4Lindvall': [565], 'J.M.': [566, 779, 804], 'Blomberg': [567], 'K.E.': [568], 'Valiaho': [569], 'J.': [570, 789, 1102, 1177, 3059, 3465], 'Vargas': [571], 'L.': [572, 711], 'Heinonen': [573], 'J.E.': [574], 'Berglof': [575], 'A.': [576, 1097], 'Mohamed': [577, 1050], 'A.J.': [578, 1051], 'Nore': [579, 1052], 'B.F.': [580, 1053], 'Vihinen': [581, 1054], 'M.': [582, 628, 717, 1055, 1176, 3051, 3055, 3464], 'Smith': [583, 631], 'C.I.': [584, 632, 1045], '2005;': [587, 1122], '203:': [588], '200-215Crossref': [589], '(161)': [592], 'Scholar).': [594, 680, 732, 815, 1064, 1129, 1251, 1451, 3539, 4234], 'importance': [596], 'evident': [600], 'from': [601, 1775, 1787, 2015, 2024, 2191, 2422, 2619, 4262, 4389], 'fact': [603], 'mutations': [605], 'cause': [608], 'X-linked': [609, 698], 'agammaglobulinemia,': [610], 'human': [612, 1462], 'disease': [613], 'defects': [616], 'maturation': [621], 'mature': [623], 'function': [626, 741], '(5Vihinen': [627], 'Mattsson': [629, 1048], 'P.T.': [630, 1049], 'Front.': [633], 'Biosci.': [634], '5:': [636], 'D917-D928Crossref': [637], '6Tsukada': [641], 'Saffran': [643, 702], 'D.C.': [644, 703], 'Rawlings': [645, 1228, 3516], 'D.J.': [646, 701, 1194, 1229, 3482, 3517], 'Parolini': [647], 'O.': [648], 'Allen': [649], 'R.C.': [650], 'Klisak': [651], 'I.': [652, 3049], 'Sparkes': [653], 'R.S.': [654], 'Kubagawa': [655], 'H.': [656, 1198, 1221, 3486, 3509], 'Mohandas': [657], 'T.': [658, 1174, 3462], 'Quan': [659], 'Belmont': [661], 'J.W.': [662], 'Cooper': [663], 'M.D.': [664], 'Conley': [665], 'M.E.': [666], 'Cell.': [669], '1993;': [670, 725], '72:': [671], '279-290Abstract': [672], 'Full': [673, 1183, 1185, 1242, 1244, 3471, 3473, 3530, 3532], 'Text': [674, 1184, 1186, 1243, 1245, 3472, 3474, 3531, 3533], 'PDF': [675, 1187, 1246, 3475, 3534], '(1168)': [678], 'A': [681, 1309, 1638, 1659, 1674, 2935, 3581, 4078], 'point': [683, 1996], 'mutation': [684, 3756], 'mice': [686], 'results': [687, 840, 3141, 4008], 'similar,': [690], 'albeit': [691], 'milder,': [692], 'deficiency': [695], 'known': [696, 1082, 1157, 1273], 'as': [697, 831, 1092, 1754, 1798, 1997, 2113, 2602, 3164, 3250, 3710, 3969, 3971, 4041, 4043], 'immunodeficiency': [699], '(7Rawlings': [700], 'Tsukada': [704], 'Largaespada': [706], 'D.A.': [707], 'Grimaldi': [708], 'J.C.': [709, 806], 'Cohen': [710], 'Mohr': [712], 'R.N.': [713], 'Bazan': [714], 'J.F.': [715], 'Howard': [716], 'Copeland': [718], 'N.G.': [719], 'Jenkins': [720], 'N.A.': [721], 'Science.': [724, 807, 1211, 3499], '261:': [726], '358-361Crossref': [727], '(782)': [730], 'indispensable': [734], 'requirement': [735], 'for': [738, 749, 850, 1333, 1408, 1413, 1473, 1901, 1993, 2168, 2176, 2278, 2334, 2510, 2541, 2576, 2612, 2647, 2692, 2752, 2805, 2844, 3024, 3186, 3203, 3221, 3424, 3560, 3618, 3644, 3743, 3763, 3785, 3815, 3860, 3866, 3958, 3967, 4114, 4496], 'humans': [743], 'makes': [744], 'promising': [747], 'target': [748], 'treatment': [750], 'inflammatory': [752], 'diseases': [753], 'involve': [755], 'inappropriate': [756], 'activation.': [759], 'BTK-mediated': [761], 'signaling': [762, 772, 1088, 1136], 'events': [763], 'link': [765], 'receptor': [768, 931], 'ligation': [769, 817, 932], 'downstream': [771, 900], 'have': [773, 3438, 3669, 4092], 'been': [774, 3439], 'well': [775, 998, 1138, 1802, 2006, 3970, 4042], '(8Dal': [777], 'Porto': [778, 803], 'Gauld': [780], 'S.B.': [781, 801], 'Merrell': [782], 'K.T.': [783], 'Mills': [784], 'D.': [785, 1436, 3032], 'Pugh-Bernard': [786], 'A.E.': [787], 'Cambier': [788, 805], 'Mol.': [790], '2004;': [792, 1423], '41:': [793], '599-613Crossref': [794], '(420)': [797], '9Gauld': [800], 'Dal': [802], '2002;': [808, 1105], '296:': [809], '1641-1642Crossref': [810], '(197)': [813], 'Upon': [816, 885], 'receptor,': [822, 829], 'kinases': [825, 852, 3020], 'associated': [826], 'such': [830, 1854], 'Lyn,': [832, 917], 'are': [833, 1081, 2553, 2840, 3249, 3283, 3792, 3821, 4013, 4458], 'enzymes': [836], 'activated.': [837], 'residues': [845, 1262, 3727], 'become': [847], 'docking': [848], 'sites': [849, 1158, 3436, 4156], 'including': [853, 902, 1161, 1283, 3445], 'phosphatidylinositol': [854, 858, 861, 865, 869, 919], '3-kinase.': [855], 'Once': [856], 'recruited,': [857], '3-kinase': [859], 'phosphorylates': [860], '4,5-bisphosphate': [862], 'form': [864, 4017, 4024, 4462, 4468], '3,4,5-bisphosphate.': [866], 'Formation': [867], '3,4,5-bisphosphate': [870], 'recruits': [872], 'plasma': [876], 'membrane': [877], 'via': [878, 893, 3981], 'interaction': [879, 4153], 'pleckstrin': [882, 951], 'domain.': [884, 985], 'recruitment': [886, 910], 'membrane,': [889], 'activated': [892], 'phosphorylation.': [894], 'proceeds': [896], 'phosphorylate': [898, 3725], 'several': [899, 956, 1156, 1633, 3434, 3959], 'substrates,': [901, 3949], 'phospholipase': [903], 'Cγ2,': [904], 'causing': [905], 'further': [906, 933, 3749], 'signal': [907, 927], 'propagation.': [908], 'Hence,': [909, 3684, 3718, 3802], '(together': [915], 'Syk,': [918], '3-kinase,': [920], 'molecules)': [923], 'transduces': [924], 'initial': [926, 2778, 2787, 3351, 3939], 'downstream,': [934], 'resulting': [935], 'elevation': [937], 'intracellular': [939], 'Ca2+': [940, 1091], 'followed': [941], 'by': [942, 1517, 1909, 1983, 2009, 2033, 2047, 2063, 2087, 2290, 2592, 2654, 2669, 2870], 'alterations': [943, 1266], 'gene': [945, 1077, 1466], 'expression.': [946], 'In': [947, 1293, 1800, 2834, 4235], 'addition': [948, 2703], 'domain,': [953, 969, 980], 'has': [955, 1149, 1155, 3433, 3804], 'domains': [958, 991], 'can': [960, 2616, 2635, 3126], 'mediate': [961], 'protein-protein': [962], 'interactions.': [963], 'These': [964, 1391, 3121, 3834, 3954], 'include': [965], '3': [973, 1514, 1945, 2192, 2374, 2394, 2621, 4390], 'domain': [974, 1002, 1172, 3450, 3460], '(SH3),': [975], '2': [979, 1706, 1837, 1948, 1954, 3008, 4264], 'C-terminal': [983], 'these': [989, 1261, 2478, 2835, 4236], 'yet': [997, 1150], 'understood.': [999], 'However,': [1000], 'structure': [1003], 'conserved': [1007], 'among': [1008], 'members': [1009], 'which': [1017, 4064, 4082, 4136], 'includes': [1019], 'Tec,': [1020], 'Itk,': [1021], 'Bmx,': [1022], 'Txk': [1024], '(10Lewis': [1025], 'C.M.': [1026], 'Broussard': [1027], 'C.': [1028, 1231, 3519], 'Czar': [1029], 'M.J.': [1030], 'Schwartzberg': [1031, 1100, 1117], 'P.L.': [1032, 1101, 1118], '2001;': [1036, 1057], '13:': [1037], '317-325Crossref': [1038], '(84)': [1041], '11Smith': [1044], 'Islam': [1046], 'T.C.': [1047], 'Bioessays.': [1056], '23:': [1058, 1123], '436-446Crossref': [1059], '(232)': [1062], 'Each': [1065], 'demonstrates': [1072], 'different': [1074, 2717], 'pattern': [1075, 2762, 4071], 'expression,': [1078, 1479], 'but': [1079, 2999], 'all': [1080], 'play': [1084], 'mechanisms': [1089], 'involving': [1090, 2631], 'second': [1094], 'messenger': [1095], '(12Takesono': [1096], 'Finkelstein': [1098, 1113], 'L.D.': [1099, 1114], 'Cell': [1103], 'Sci.': [1104], '115:': [1106], '3039-3048Crossref': [1107], '13Berg': [1111], 'L.J.': [1112], 'Lucas': [1115], 'J.A.': [1116], 'Annu.': [1119], '549-600Crossref': [1124], '(267)': [1127], 'Although': [1130], 'established,': [1139], 'described.': [1153, 1998], 'Tyr223': [1168, 3446], 'SH3': [1171, 3449], '(14Kurosaki': [1173, 3461], 'Kurosaki': [1175, 3463], 'Biol.': [1178, 3466], 'Chem.': [1179, 3467], '272:': [1181, 3469], '15595-15598Abstract': [1182, 3470], '(118)': [1190, 3478], '15Rawlings': [1193, 3481], 'Scharenberg': [1195, 1232, 3483, 3520], 'A.M.': [1196, 1233, 3484, 3521], 'Park': [1197, 3485], 'Wahl': [1199, 1222, 3487, 3510], 'M.I.': [1200, 1223, 3488, 3511], 'Lin': [1201, 3489], 'Kato': [1203, 3491], 'R.M.': [1204, 3492], 'Fluckiger': [1205, 3493], 'A.C.': [1206, 3494], 'Kinet': [1209, 1234, 3497, 3522], 'J.P.': [1210, 1235, 3498, 3523], '1996;': [1212, 1239, 3500, 3527], '271:': [1213, 3501], '822-825Crossref': [1214, 3502], '(381)': [1217, 3505], '16Park': [1220, 3508], 'Afar': [1224, 3512], 'D.E.': [1225, 3513], 'Turck': [1226, 3514], 'C.W.': [1227, 3515], 'Tam': [1230, 3518], 'Immunity.': [1238, 3526], '4:': [1240, 3528], '515-525Abstract': [1241, 3529], '(256)': [1249, 3537], 'Little': [1252], 'quantitative': [1253], 'information': [1254], 'available': [1256], 'regarding': [1257, 2780], 'how': [1258, 3360], 'linked': [1264], 'Furthermore,': [1270, 3105, 3811], 'little': [1271, 3805], 'about': [1274], 'reaction,': [1282, 1880], 'parameters': [1286, 2804, 4102], 'this': [1294, 1322, 2083, 2786, 2973, 3350, 3656, 4437], 'study,': [1295], 'we': [1296, 3658, 3759, 3878, 3930], 'report': [1297], 'quantitative,': [1300], 'mechanism.': [1308, 2538], '33P-based': [1310], 'filtration': [1311], 'assay': [1312, 1323, 1763, 1812, 1852, 2156, 2264, 2699, 2942, 2974, 3084], 'developed': [1315, 1761, 2399, 2949], 'monitor': [1317, 1765, 2944], 'phosphorylation,': [1320], 'utilized': [1325], 'significance': [1329], 'catalysis.': [1334], 'It': [1335, 2615, 3777], 'ascertained': [1337, 4492], 'increases': [1351], 'activity': [1353, 1990, 2947, 3026, 3107, 3355, 3427, 3648, 3671, 3695, 3746, 3841], '5–10-fold.': [1354], 'characterized.': [1364], 'displays': [1369], 'sequential': [1371, 3991], '(i.e.': [1372, 2532, 3990, 4316], 'complex)': [1374], 'modest': [1379], 'negative': [1380, 4196], 'communication': [1381, 2586, 4000], 'occurs': [1382], 'findings': [1392, 3122, 3835], 'provide': [1393, 2776], 'description': [1398, 4060], 'useful': [1407], 'characterizing': [1409], 'therapeutic': [1414], 'benefit': [1415], '(17Vassilev': [1416], 'A.O.': [1417, 1432], 'Uckun': [1418, 1439], 'F.M.': [1419, 1440], 'Pharm.': [1421], 'Des.': [1422], '1757-1766Crossref': [1425], '(22)': [1428], '18Vassilev': [1431], 'Tibbles': [1433], 'H.E.': [1434], 'DuMez': [1435], 'Venkatachalam': [1437], 'T.K.': [1438], 'Drug': [1442, 4164, 4214], 'Targets.': [1443], '2006;': [1444], '7:': [1445], '327-343Crossref': [1446], '(12)': [1449], 'Protein': [1452], 'Expression': [1453], 'Purification—All': [1455], 'experiments': [1456, 2682, 3955, 4355], 'His-tagged,': [1460], 'full-length': [1461], 'cloned': [1468], 'into': [1469, 1777], 'vector': [1470], 'PVL': [1471], '1392': [1472], 'expression': [1474], 'baculovirus.': [1476], 'For': [1477, 1523, 2171, 2882], 'protein': [1478, 1726, 3019], 'Sf9': [1480, 1528], 'grown': [1483], 'density': [1486], '1.0': [1488], '1.5': [1490], '×': [1491, 1521, 1604], '106': [1492, 3793], 'cells/ml': [1493], 'SF900': [1495], 'medium': [1496], '27': [1498], '°C.': [1499, 1731], 'Cells': [1500, 1577], 'infected': [1502, 1527], 'recombinant': [1504], 'virus': [1505], '0.5': [1507, 1552], 'multiplicity': [1508], 'infection': [1510], 'harvested': [1513], 'days': [1515], 'post-infection': [1516], 'centrifugation': [1518], '1000': [1520, 4392], 'g.': [1522], 'purification': [1524], 'pellets': [1530], 'resuspended': [1532], 'lysis': [1534, 1571], 'buffer': [1535, 1694, 1813, 1853, 2157, 2265, 2319, 2700], '(50': [1536, 1639, 1695], 'mm': [1537, 1542, 1545, 1550, 1640, 1645, 1650, 1653, 1662, 1677, 1696, 1701, 1704, 1707, 1815, 1820, 1827, 1830, 1838, 1939, 2300, 2321, 2326], 'Hepes': [1538, 1641, 1697], 'pH': [1539, 1642, 1698, 1817, 2323], '8.0,': [1540, 1643, 2324], '300': [1541, 1644], 'NaCl,': [1543, 1646, 1702, 1950, 2327], 'dithiothreitol,': [1546, 1651, 1708], '10%': [1547, 1647, 1928], 'glycerol,': [1548, 1648], 'imidazole,': [1551, 1667, 1816], 'μl/ml': [1553], 'Benzonase': [1554], 'nuclease': [1555], '(Novagen,': [1556], 'San': [1557, 2245, 2360], 'Diego,': [1558, 2246, 2361], 'CA),': [1559], 'complete': [1561, 3610], 'protease': [1562], 'tablet/50': [1563], 'ml': [1564, 1569], 'buffer)': [1566], '400': [1568, 1676], 'buffer/5': [1572], 'liters': [1573], 'pellet.': [1576], 'lysed': [1580], 'single': [1583], 'pass': [1584], 'through': [1585, 3100], 'microfluidizer': [1587], '(Microfluidics': [1588], 'Corporation,': [1589], 'Newton,': [1590], 'MA)': [1591, 1926, 1976], '<11,000': [1593], 'psi': [1594], 'on': [1595, 1770, 2057, 2166, 2958, 3543, 3757, 3807, 4201, 4357], 'ice.': [1596], 'Lysed': [1597], 'material': [1598], 'centrifuged': [1601], '25,000': [1603], 'g': [1605], 'Beckman': [1608], 'JA14': [1609], 'rotor,': [1610], 'supernatant': [1613], 'retained.': [1615], 'Material': [1616], 'applied': [1618, 1683], 'pre-equilibrated': [1621, 1690], 'His': [1622], 'trap': [1623], 'FF': [1624], 'crude': [1625], 'column': [1626, 1634, 1689], '(GE': [1627, 1931, 2404], 'Healthcare).': [1628, 2405], 'Columns': [1629], 'washed': [1631, 1655, 1944, 2373, 2393], 'volumes': [1635], 'Buffer': [1637, 1658, 1671], 'imidazole),': [1654], 'again': [1656], '40': [1661, 1843], '(rather': [1663], 'mm)': [1666], 'eluted': [1669], '(Buffer': [1673], 'imidazole).': [1678], 'Fractions': [1679], 'pooled': [1681], 'Superdex': [1686, 1692], '200': [1687, 1693, 1823, 4250], '26/60': [1688], '7.6,': [1699], '250': [1700, 2255], '10': [1703, 1881, 1892, 2428, 2693, 3204, 3376, 3390], 'MgCl2,': [1705, 1828], '5%': [1709, 2332, 2348], 'glycerol).': [1710], 'Pure': [1711], 'fractions': [1712], 'retained': [1714], 'concentrated': [1716], 'Amicon': [1719], 'stirred': [1720], '2.2': [1723], 'mg/ml': [1724, 1833], 'final': [1725, 1857, 1889, 2624], 'stored': [1728], '–80': [1730], 'constructured': [1735], 'QuikChange': [1738], 'Mutagenesis': [1739], 'Kit': [1740], '(Stratagene,': [1741], 'La': [1742], 'Jolla,': [1743], 'CA)': [1744, 2362], 'expressed': [1747, 2638], 'purified': [1749], 'same': [1752], 'manner': [1753], 'wild': [1755, 3766, 3789, 3901], 'Enzyme': [1759, 4161, 4175, 4211, 4225], 'Assay—We': [1760], 'product': [1767, 2076, 2105, 2977, 2994, 3095, 3201, 3301, 3396], 'formation': [1768, 2106, 2978, 2995, 3096, 3202, 3397, 3691], 'based': [1769], 'incorporation': [1772], '33PO4': [1774], '[33P]ATP': [1776, 1789], 'biotin-tagged': [1779], 'peptide.': [1781], 'subsequently': [1785], 'isolated': [1786], 'unreacted': [1788], 'streptavidin-coated': [1791, 2959], 'beads.': [1792], 'standard': [1794], 'experimental': [1795, 2110], 'protocol': [1796], 'follows.': [1799], 'each': [1801, 1994, 2005, 2131, 2511, 2663, 2736, 2747, 2806, 2866], '96-well': [1805, 1917], 'V': [1806], 'bottom': [1807], 'plate': [1808, 1923], '(Greiner,': [1809], 'Monroe,': [1810], 'NC),': [1811], '(8': [1814], '7.2,': [1818], 'glycerol': [1821], '2-phosphate,': [1822], 'EGTA,': [1825], 'MnCl2,': [1831], '0.1': [1832], 'bovine': [1834, 2349], 'serum': [1835, 2350], 'albumin,': [1836], 'dithiothreitol)': [1839], 'combined': [1841], 'μl': [1844, 1882, 1912], 'mixture': [1847], 'substrates': [1849, 2705], 'dissolved': [1850, 1933], 'μCi': [1861], '[33P]ATP,': [1863], '3–540': [1864], 'ATP,': [1866, 2559, 3972], '1–180': [1868], '(biotin-Aca-AAAEEIYGEI-NH2)': [1873], '(see': [1874, 2961, 4400, 4439], 'Fig.': [1875, 2963, 3369, 3408, 3775, 3832, 3875, 3894, 4019, 4027, 4464, 4471], '1).': [1876, 3120, 3683, 3717, 4368], 'initiate': [1878, 2707], 'added': [1886], 'concentration': [1890, 2065, 2073, 2118, 2133, 2185, 2749, 3213, 3367, 3406, 4512], 'nm.': [1893, 3391], 'reaction': [1895, 1905, 2089, 2215, 2287, 2987, 3362, 3597, 3607, 3940, 3978, 4404], 'incubated': [1897, 2262, 2340, 2378, 3173, 3559], '30': [1899, 2423, 3378], '°C': [1900, 2344], '15': [1902, 2169, 3799], 'min.': [1903, 2170, 3613], 'stopped': [1908], 'transferring': [1910], '25': [1911, 3101], 'sample': [1914, 2056], '1.2-μm': [1918], 'hydrophilic': [1919], 'polyvinylidene': [1920, 2309], 'difluoride': [1921, 2310], 'filter': [1922, 2059], '(Millipore,': [1924], 'Billerica,': [1925], 'containing': [1927], 'streptavidin-Sepharose': [1929], 'beads': [1930, 2960], 'Healthcare)': [1932], 'phosphate-buffered': [1935], 'saline': [1936], 'plus': [1937, 2298, 2383], '50': [1938, 2125, 2163, 2299], 'EDTA.': [1940, 2301], 'Filter': [1941], 'plates': [1942], 'times': [1946, 2375, 2395], 'm': [1949, 1955], 'twice': [1952], 'NaCl': [1956], '1%': [1958], 'phosphoric': [1959], 'acid,': [1960], 'H2O.': [1964], 'Plates': [1965], 'allowed': [1967], 'dry': [1969], 'microscint-20': [1971], '(PerkinElmer': [1972], 'Life': [1973], 'Sciences,': [1974], 'Boston,': [1975], 'added.': [1978], '[33P]phosphoproduct': [1980], 'detected': [1982], 'top-count': [1985], 'scintillation': [1986], 'counter.': [1987], 'calculated': [1992, 3879], 'data': [1995, 2198, 2234, 2434, 2448, 2482, 2493, 2516, 2661, 2767, 2790, 2909, 3288, 4032, 4457], 'corrected': [2000], 'number': [2001, 2036], 'counts': [2003, 2013, 2038], 'determined': [2008, 2135, 3856, 3943, 4484], 'subtracting': [2010], 'background': [2012], '(obtained': [2014], 'experiment': [2017, 3345, 4012], 'absence': [2021, 2272, 3177, 3233], 'S1)': [2023], 'measured': [2026, 2214], 'counts.': [2027], 'value': [2029, 2084, 2672, 3966, 4193], 'divided': [2032, 2086], 'total': [2035], 'originally': [2041], 'present': [2042], 'solution': [2045, 2069, 2294], '(determined': [2046], 'spotting': [2048], 'counting': [2050], 'equivalent': [2052], 'volume': [2053], 'unwashed': [2055], 'plate)': [2060], 'multiplied': [2062], 'give': [2071], 'phosphorylated': [2075, 2954, 3215], 'formed.': [2077], 'obtain': [2079], 'velocity,': [2082, 2216, 2221], 'time': [2090, 2101, 2149, 2988, 3089, 3198, 3220, 3315, 3334, 3372], 'experiment.': [2093, 2807], 'Time': [2094], 'Course': [2095], 'Experiments': [2096], 'Km': [2098, 2175, 2222, 2508, 2732, 3762, 3783, 3813, 3859, 3965, 4113, 4124, 4383, 4478], 'Determination—When': [2099], 'course': [2102, 2150, 2989, 3199, 3316, 3335], 'determined,': [2108], 'conditions': [2111, 2154], 'described': [2114], 'above': [2115], '100': [2123, 2159, 2275, 3183, 3242], 'μm,': [2126, 2431, 3800, 3829, 4120], 'respectively.': [2127, 2432, 2472, 3801], 'velocity': [2129, 2197, 2519, 2713, 3312, 3331, 3941], 'linear': [2137, 2980, 2998, 3088, 3099, 3275], 'least': [2138, 2203], 'squares': [2139, 2204, 2876], 'methods.': [2140], 'If': [2141, 2473], 'preincubated': [2144, 2689, 3229, 3703], 'Mg2+/ATP': [2146, 3079, 3564, 3584, 3633, 3705], 'prior': [2147, 2701, 3080, 3192], 'experiments,': [2151, 2891, 4237], 'preincubation': [2153, 3075, 3092, 3118, 3429, 3651], 'nm': [2164, 2256, 3205, 4393], 'ice': [2167], 'determination': [2172], 'Y551F,': [2182, 3625], 'varied': [2187, 2415, 3364, 4375, 4388, 4507], 'quarter-log': [2189, 2420], 'scale': [2190], '540': [2194, 2425], 'fit': [2200, 2496, 2524, 2794, 2803, 2812, 2913, 2926, 3277, 4101, 4407], 'nonlinear': [2202], 'methods': [2205, 2798], 'Michaelis-Menten': [2208, 2452, 2499, 2726, 4023], 'equation,v=Vmax[S]Km+[S](Eq.1)': [2209], 'where': [2210, 2549, 2604, 3937], 'v': [2211], 'Vmax': [2217, 2506, 2734, 4130], 'maximal': [2220, 3025], 'Michaelis': [2225, 2554, 2597], 'constant,': [2226], '[S]': [2228], 'concentration.': [2232, 2513, 2863], 'All': [2233], 'analysis': [2235, 3936, 4399, 4438, 4474, 4488], 'GraphPad': [2239], 'Prism': [2240], '4.0': [2241], '(GraphPad': [2242], 'Software,': [2243], 'Inc.,': [2244, 4181, 4231], 'CA).': [2247], 'Blot': [2250], 'Analysis—For': [2251, 2407], 'blot': [2253, 2313, 3150, 3195], 'analysis,': [2254, 2410, 2788], 'presence': [2269, 3180, 3238], '(+)': [2270, 3181], '(–)': [2273, 3178], '5': [2281, 3190, 4465], 'min': [2282, 2694, 3102, 3191, 3379, 3562], 'room': [2284, 2696], 'temperature.': [2285], 'quenched': [2289], 'adding': [2291], '1:1': [2293], 'loading': [2296, 3166], 'dye': [2297], 'After': [2302], 'gel': [2303], 'electrophoresis,': [2304], 'samples': [2305], 'transferred': [2307], 'membranes.': [2311], 'blocked': [2316], 'TBST': [2318, 2346, 2382], '(25': [2320], 'Tris': [2322], '125': [2325], '0.1%': [2328], 'Tween': [2329], '20)': [2330], 'Blotto': [2333], 'h.': [2336], 'Samples': [2337], 'overnight': [2341], '4': [2343, 4020], 'albumin': [2351], '1:500': [2354], 'monoclonal': [2355], 'anti-phospho-Tyr551': [2356], '(BD': [2358], 'Pharmingen,': [2359], '1:1000': [2364], 'polyclonal': [2365], 'anti-BTK': [2366], '(Cell': [2367], 'Signaling,': [2368], 'Beverly,': [2369], 'MA).': [2370], 'Blots': [2371], 'TBST,': [2377, 2397], 'h': [2380], '1:8000': [2384], 'anti-mouse': [2386], 'anti-rabbit': [2388], 'horseradish': [2389], 'peroxidase-conjugated': [2390], 'secondary': [2391], 'antibodies,': [2392], 'ECL': [2401], 'Plus': [2402], 'reagents': [2403], 'Two-substrate': [2406, 3920], 'two-substrate': [2409, 3934, 4487], 'matrix': [2418], 'intervals': [2421], '180': [2430], 'evaluated': [2436, 2668, 2869], 'analyzed': [2438, 2722], 'following': [2441, 3117, 3428, 3650], 'procedure.': [2442], 'First,': [2443], 'ascertain': [2445, 2504, 2729, 2800, 3419, 4431], 'if': [2446, 3420], 'displayed': [2449], 'expected': [2451, 2475, 4066], 'behavior,': [2453], 'double': [2454], 'reciprocal': [2455, 2765], 'plots': [2456, 2479, 2739, 4075], '1/v': [2458, 2741], 'versus': [2459, 2714, 2742, 2885, 2889, 3219, 4449, 4454], '1/[S1]': [2460], '1/[ATP]': [2462], 'generated': [2464, 2745], 'constant': [2466, 2573, 2598, 2601, 2609], 'values': [2467, 2509, 2520, 3784, 3814, 4479], 'linearity': [2476, 2753], 'observed,': [2481], 'assumed': [2484], 'high': [2488, 3694, 3745], 'quality': [2489, 2757], 'untransformed': [2492], 'equation': [2500, 2540, 2630, 2646, 2727], '(Equation': [2501, 2899, 2933, 4039, 4048, 4414, 4419, 4428], '1)': [2502, 3961], 'apparent': [2505, 2731], 'entire': [2515, 2789, 2879, 4031], 'set': [2517], 'globally': [2523, 2793, 2811], 'equations': [2526], 'describing': [2527], 'sequential)': [2533], 'ping-pong': [2536, 2649, 4045], 'given': [2547, 2591, 2653, 4067], 'by,v=Vmax[ATP][S1][ATP][S1]+[ATP]Km,S1+[S1]Km,ATP+Km,ATPKa,S1(Eq.2)': [2548], 'Km,ATP': [2552, 2632], 'constants': [2555, 2843, 4261], 'respectively,': [2560], 'saturating': [2562], 'substrate,': [2567], 'Ka,S1': [2569, 2634], 'association': [2572], 'free': [2577, 2613, 4521], 'Here': [2579], 'α': [2581, 4145], 'factor,': [2582, 4146], 'measure': [2584, 4148], 'sites,': [2589], 'ratio': [2594], 'dissociation': [2600, 2608], 'follows,α=Km,S1Ka,S1=Km,ATPKa,ATP(Eq.3)': [2603], 'Ka,ATP': [2605], 'observed': [2618, 3649, 4255], 'Equation': [2620, 2655], 'term': [2625], 'alternatively': [2636], 'terms': [2640], 'Km,S1': [2642], 'Ka,ATP.': [2644], '4.v=Vmax[ATP][S1][ATP][S1]+[ATP]Km,S1+[S1]Km,ATP(Eq.4)': [2656], 'goodness-of-fit': [2658], 'models': [2666, 4409], 'reduced': [2671, 2873], 'absolute': [2675], 'sum': [2676, 2874], 'squares.': [2678], 'Substrate': [2679, 4298], 'Analysis—In': [2681], 'temperature': [2697], 'reaction.': [2709], 'Data': [2710, 2808], 'sets': [2711, 2791, 2809, 2910, 4033], 'inhibitor': [2718, 2748, 2770, 2845, 2862, 4365], 'individually': [2723], 'under': [2735], 'condition.': [2737], 'Reciprocal': [2738], '1/S': [2743], 'confirm': [2755], 'data.': [2760, 2906, 3280], 'plot': [2766], 'multiple': [2769, 3287], 'idea': [2779], 'inhibition.': [2784, 4435], 'Following': [2785, 3091, 4397], 'nonlinear,': [2796], 'least-squares': [2797], 'best': [2802, 3276, 4100], 'model': [2817, 2822, 2829, 2867, 2898, 2920, 2932, 4038, 4047, 4055, 4107, 4424], 'inhibition,v=Vmax[S]Km(1+[1]/Ki1)+[S](Eq.5)': [2820], 'uncompetitive': [2824, 2919, 4417], 'inhibition,v=Vmax[S]Km+[S](1+[1]/Ki2)(Eq.6)': [2825], 'general': [2828, 4423], 'mixed': [2831], '(noncompetitive)': [2832], 'inhibition.v=Vmax[S]Km(1+[1]/Ki1)+[S](1+[1]/Ki2)([Eq.7]+)': [2833], 'equations,': [2836], 'Ki1': [2837], 'Ki2': [2839], 'inhibition': [2842, 2931, 4253, 4344, 4413, 4418, 4427, 4448, 4453], 'alone': [2851], '(Ki1)': [2852], 'enzyme-substrate': [2855], '(Ki2)': [2857], '[I]': [2859], 'Goodness-of-fit': [2864], 'examining': [2871], 'global': [2880], 'fit.': [2881], 'it': [2892, 3071, 4238], '5)': [2900], 'sufficient': [2902], 'describe': [2904], 'remaining': [2908], 'simple': [2915, 2918], 'demonstrated': [2922, 3706], 'significantly': [2924, 3729], 'mixed,': [2929], 'noncompetitive': [2930, 4426, 4452], '7).': [2934], 'Lag-phase': [2936], 'Product': [2939], 'Formation—A': [2940], 'capture': [2952], 'supplemental': [2962, 4026, 4470], '"Experimental': [2966, 4401, 4440], 'Procedures").': [2967], 'question': [2970], 'addressed': [2971], 'time.': [2982], 'An': [2983], 'examination': [2984, 3358], 'rather': [3000], 'occurred': [3001, 3980], 'distinct': [3004], 'lag': [3005, 3013], 'phase': [3006, 3014], '(Figs.': [3007], '3).': [3010, 3638, 4540], 'Because': [3011], 'frequently': [3016], 'require': [3022], '(19Barker': [3027], 'S.C.': [3028], 'Kassel': [3029], 'D.B.': [3030], 'Weigl': [3031], 'Huang': [3033], 'X.': [3034], 'Luther': [3035], 'M.A.': [3036], 'Knight': [3037], 'W.B.': [3038], 'Biochemistry.': [3039], '1995;': [3040], '34:': [3041], '14843-14851Crossref': [3042], '20Moarefi': [3048], 'LaFevre-Bernt': [3050], 'Sicheri': [3052], 'F.': [3053], 'Huse': [3054], 'Lee': [3056], 'C.H.': [3057], 'Kuriyan': [3058], 'Miller': [3060], 'W.T.': [3061], 'Nature.': [3062], '385:': [3064], '650-653Crossref': [3065], '(541)': [3068], 'Scholar),': [3070, 4184], 'would': [3085, 4086, 4091], 'produce': [3086], 'course.': [3090], 'Mg2+/ATP,': [3094], 'indeed': [3098], '(Fig.': [3103, 3575, 3598, 3635, 3680, 3714, 4076, 4367], '2).': [3104, 3580], '3-fold': [3115, 4517], 'greater': [3116, 3593, 3911], '(Table': [3119, 4108, 4489, 4539], 'suggest': [3123], 'autophosphorylate': [3127], 'might': [3133], 'activity.FIGURE': [3136], '3BTK': [3137], 'A,': [3148], '(top)': [3158], '(bottom)': [3163], 'control.': [3167], '(or': [3169], 'Y551F)': [3171], 'analysis.': [3196], 'B,': [3197], 'Y551F.': [3209], 'Shown': [3210], '(μm)': [3218], '(BTK': [3223], 'No': [3224, 3235, 3253, 3260], 'preinc)': [3225, 3236], '(Y551F': [3234, 3239], 'Preinc)': [3240], 'ATP.': [3244, 3953], 'correlation': [3246], 'coefficients': [3247], '(r)': [3248], 'follows:': [3251], 'Preinc:': [3254, 3267], 'r': [3255, 3262, 3268], '0.980;': [3257], 'preinc:': [3261], '0.985;': [3264], '0.999.': [3270], 'Solid': [3271], 'lines': [3272], 'represent': [3273], 'Error': [3281], 'bars': [3282], 'S.D.': [3285], 'points.View': [3289], 'Large': [3290], 'Image': [3291], 'Figure': [3292], 'ViewerDownload': [3293], 'Hi-res': [3294], 'image': [3295], 'Download': [3296], '(PPT)TABLE': [3297], '1Velocity': [3298], 'formationBTK': [3302], 'formPreincubation': [3303], 'statevnm/minWild': [3304], 'typeNo': [3305], 'preincubation120': [3306], '5aEstimate': [3308], 'average': [3311, 3330], 'since': [3313, 3332], 'nonlinear.Wild': [3318], 'typePreincubation350': [3319], '9Y551FNo': [3321], 'preincubation24': [3322], '1Y551FPreincubation25': [3324], '1a': [3326], 'Estimate': [3327], 'nonlinear.': [3337], 'Open': [3338, 4284], 'table': [3339, 4285], 'new': [3342, 4288], 'tab': [3343, 4289], 'Another': [3344], 'conducted': [3348], 'rate': [3363, 3393, 4141], '(supplemental': [3368, 3407, 3774, 3831, 3874, 3893], 'S2).': [3370, 3409], 'Reaction': [3371], 'courses': [3373], 'collected': [3381], '1.25,': [3386], '2.5,': [3387], '5,': [3388], 'linearly': [3402], 'increasing': [3404], 'Phosphorylation': [3410], 'Enhances': [3413], 'Activity—We': [3415], 'next': [3416, 3931, 4301], 'sought': [3417, 4302], 'responsible': [3423, 3643, 3742], 'enhancing': [3425], 'Mg2+/ATP.': [3431], 'proposed': [3440], 'functionally': [3443], 'important': [3444, 3689], 'Attention': [3540], 'focused': [3542, 4356], 'Tyr551.': [3544], 'Using': [3545, 4297], 'phospho-Tyr551-specific': [3547], 'antibody,': [3548, 3569], 'probed': [3554], 'blotting.': [3557], 'reacted': [3565], 'strongly': [3566], 'whereas': [3570, 4121, 4385], 'untreated': [3571, 3711, 3786, 3816, 3867], '3A,': [3576, 3599, 3636], 'lanes': [3577], 'longer,': [3582], '5-min': [3583], 'incubation': [3585, 3634], 'produced': [3586, 4094], 'level': [3588, 3674], '1-min': [3596], 'lane': [3600, 3637], '4),': [3601], 'specific': [3617], 'phospho-Tyr551': [3619], 'because': [3620, 4252], 'mutant,': [3623], 'react': [3628], 'after': [3632], 'Is': [3639], 'enhanced': [3646], 'Mg2+/ATP?': [3653], 'address': [3655], 'question,': [3657], 'studied': [3659, 4245], 'mutant.': [3663], 'lower': [3676, 4248], 'preactivated': [3678, 3788, 3818, 3869, 3900], '3B,': [3681, 3715], 'Table': [3682, 3716], 'identical': [3707, 3865], 'behavior': [3709], 'does': [3721], 'appear': [3723], 'any': [3726], 'activity,': [3732], 'sole': [3738], 'state.': [3747], 'effect': [3752, 3806, 4197], 'compared': [3760], 'S3A).': [3776, 3833, 3895], '23': [3795], '90': [3797], 'preactivation': [3803], '176': [3822], '36': [3824], '124': [3826], '17': [3828], 'respectively': [3830], 'indicate': [3836], 'decreased': [3839], 'unlikely': [3846], 'attributable': [3847], 'defect': [3850], 'essentially': [3864], '3B).': [3876], 'Finally,': [3877], 'catalytic': [3881, 3897, 3926], 'efficiency': [3882, 3898], '(kcat/Km)': [3883], 'respect': [3885], '(3.7': [3904], '0.2': [3906], 'mm–1': [3907, 3918], 's–1)': [3908], '(0.47': [3915], '0.03': [3917], 's–1).': [3919], 'Kinetic': [3921, 4295], 'Analysis—To': [3922], 'varying': [3945], 'undertaken': [3957, 4338], 'reasons:': [3960], 'determine': [3963], '2)': [3973, 4040, 4109], 'establish': [3975], 'mechanism),': [3992], '3)': [3994, 4049, 4490], 'degree': [3998, 4151], 'two': [4003], 'representative': [4011], 'shown': [4014, 4459], 'double-reciprocal': [4016, 4074, 4461], 'S4.': [4028], 'Fitting': [4029], 'provided': [4056], 'much': [4058], 'data,': [4063], 'intersecting': [4069], 'line': [4070, 4097], '4).': [4077], 'pingpong': [4079], 'mechanism,': [4080], 'bind': [4088, 4325], 'simultaneously,': [4090], 'instead': [4093], 'parallel': [4096], 'pattern.': [4098], '7': [4134], 'nm/min,': [4135], 'corresponds': [4137], 'turnover': [4140], '8.4/min.': [4143], '(21Copeland': [4157, 4207], 'R.A.': [4158, 4208], 'Evaluation': [4159, 4209], 'Inhibitors': [4162, 4212], 'Discovery.': [4165, 4215], 'John': [4166, 4177, 4216, 4227], 'Wiley': [4167, 4178, 4217, 4228], 'Sons,': [4169, 4219], 'Hoboken,': [4170, 4220], 'NJ2005Google': [4171, 4221], '22Segel': [4173, 4223], 'I.H.': [4174, 4224], 'Kinetics.': [4176, 4226], 'Sons': [4180, 4230], 'New': [4182, 4232], 'York1975Google': [4183, 4233], '3.1': [4189], '1.6.': [4191], 'indicates': [4194], 'vice': [4205], 'versa': [4206], 'should': [4239], 'noted': [4241], 'higher': [4257], 'concentrations.TABLE': [4259], '2Kinetic': [4260], 'analysisSubstrateKmKaVmaxαμmnm/minATP84': [4267], '2026': [4269], '2384': [4271], '73.1': [4273, 4281], '1.6S137': [4275], '812': [4277], '1184': [4279], '1.6': [4283], 'Investigation': [4290], 'Steady': [4293], 'State': [4294], 'Mechanism': [4296], 'Analog': [4299], 'Inhibitors—We': [4300], 'required': [4323], 'random).': [4332], 'accomplish': [4334], 'this,': [4335], '(competitive,': [4345], 'noncompetitive,': [4346], 'uncompetitive)': [4348], 'inhibitors.': [4352], 'Here,': [4369], 'six': [4377], 'centered': [4379], 'their': [4381], 'respective': [4382], 'values,': [4384], 'half-log': [4395], 'intervals.': [4396], 'preliminary': [4398], 'Procedures"),': [4402, 4441], 'rates': [4405], 'pure': [4411, 4416], '5),': [4415], '6),': [4420], '7)': [4429], 'From': [4436], 'show': [4446], 'S1.': [4455], 'Representative': [4456], 'Michealis-Menton': [4467], 'S5.': [4472], 'accurately': [4475], 'reproduced': [4476], 'Ki': [4495], '0.036': [4499], '0.009': [4501], 'when': [4503], 'substrate.': [4508], 'binds': [4516], 'more': [4518], 'potently': [4519], '(ki1': [4523], '0.015': [4525], '0.005': [4527], 'μm)': [4528, 4538], 'BTK-S1': [4531], '(ki2': [4533], '0.042': [4535], '0.011': [4537], 'finding': [4542], 'co': [4544]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1985441886', 'counts_by_year': [{'year': 2024, 'cited_by_count': 3}, {'year': 2023, 'cited_by_count': 5}, {'year': 2022, 'cited_by_count': 3}, {'year': 2021, 'cited_by_count': 3}, {'year': 2020, 'cited_by_count': 2}, {'year': 2019, 'cited_by_count': 3}, {'year': 2018, 'cited_by_count': 1}, {'year': 2017, 'cited_by_count': 5}, {'year': 2016, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 3}, {'year': 2014, 'cited_by_count': 7}, {'year': 2013, 'cited_by_count': 1}, {'year': 2012, 'cited_by_count': 1}], 'updated_date': '2025-01-05T22:05:37.760955', 'created_date': '2016-06-24'}