Title: ISOENZYME COMPLEMENTS OF DIANTHUS CALLUS CULTURES: INFLUENCE OF LIGHT AND TEMPERATURE
Abstract: Callus cultures isolated from the stems of two clones of Dianthus were extracted for water‐soluble proteins and the extracts were subsequently analyzed by acrylamide gel electrophoresis. Peroxidase, acid phosphatase, esterase, and 6‐P‐gluconate dehydrogenase (6‐P‐G DH) were studied under each of four combinations of light and dark, cold (0‐5 C) and warm (25 C) environments. Marked differences in the isoenzyme patterns were observed under the four conditions with all the enzymes except 6‐P‐G DH. Acid phosphatase generally showed fewer isoenzymes than the usual stem complement, whereas the other enzymes showed the same or greater number of isoenzymes. Many of the isoenzymes from the callus tissue correlated well with those found in the stem; however, all but 6‐P‐G DH showed some different components. Of the four enzymes studied only peroxidase was recovered from the medium; these isoenzymes matched those found in the callus and in the stem. The isoenzymes in the medium were not influenced qualitatively by the growth conditions. The changes in the zymograms of the callus under the four environments were compared to the changes observed in the stem under stress environments.
Publication Year: 1970
Publication Date: 1970-02-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 14
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