Title: A Novel Assay for Erythrocyte Carbonic Anhydrase and Certain Sulfonamide Drugs Employing Spin-Labels
Abstract: A novel assay procedure has been developed for the determination of sulfonamide drugs with the general formula ArSO2NH2 where Ar = aryl (either homocyclic or heterocyclic). The assay depends on the displacement of a spin-labeled sulfonamide from the active site of bovine carbonic anhydrase B and can detect concentrations of sulfonamide as low as 0.3 µg/ml. This technique can also be used to assay low concentrations of carbonic anhydrase in hemolysates of red blood cells without prior removal of the hemoglobin or isolation of the enzyme.
Publication Year: 1972
Publication Date: 1972-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 6
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot