Membrane-embedded Synaptotagmin Penetrates cis ortrans Target Membranes and Clusters via a Novel Mechanism

Name: Work Video:
Duration: 3 min 30 s
Jihong Bai; Cynthia A. Earles; Jessica L. Lewis; Edwin R. Chapman
{'id': 'https://openalex.org/W1982257516', 'doi': 'https://doi.org/10.1074/jbc.m906729199', 'title': 'Membrane-embedded Synaptotagmin Penetrates cis ortrans Target Membranes and Clusters via a Novel Mechanism', 'display_name': 'Membrane-embedded Synaptotagmin Penetrates cis ortrans Target Membranes and Clusters via a Novel Mechanism', 'publication_year': 2000, 'publication_date': '2000-08-01', 'ids': {'openalex': 'https://openalex.org/W1982257516', 'doi': 'https://doi.org/10.1074/jbc.m906729199', 'mag': '1982257516', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/10840045'}, 'language': 'en', 'primary_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m906729199', 'pdf_url': 'http://www.jbc.org/article/S0021925819621046/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': True, 'oa_status': 'hybrid', 'oa_url': 'http://www.jbc.org/article/S0021925819621046/pdf', 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5002374231', 'display_name': 'Jihong Bai', 'orcid': 'https://orcid.org/0000-0001-6773-2175'}, 'institutions': [{'id': 'https://openalex.org/I135310074', 'display_name': 'University of Wisconsin–Madison', 'ror': 'https://ror.org/01y2jtd41', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I135310074']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Jihong Bai', 'raw_affiliation_strings': ['Department of Physiology, University of Wisconsin School of Medicine, Madison 53706 USA.'], 'affiliations': [{'raw_affiliation_string': 'Department of Physiology, University of Wisconsin School of Medicine, Madison 53706 USA.', 'institution_ids': ['https://openalex.org/I135310074']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5060704340', 'display_name': 'Cynthia A. Earles', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I135310074', 'display_name': 'University of Wisconsin–Madison', 'ror': 'https://ror.org/01y2jtd41', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I135310074']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Cynthia A. Earles', 'raw_affiliation_strings': ['From the Department of Physiology, University of Wisconsin School of Medicine, Madison, Wisconsin 53706'], 'affiliations': [{'raw_affiliation_string': 'From the Department of Physiology, University of Wisconsin School of Medicine, Madison, Wisconsin 53706', 'institution_ids': ['https://openalex.org/I135310074']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5040581517', 'display_name': 'Jessica L. Lewis', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I135310074', 'display_name': 'University of Wisconsin–Madison', 'ror': 'https://ror.org/01y2jtd41', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I135310074']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Jessica L. Lewis', 'raw_affiliation_strings': ['From the Department of Physiology, University of Wisconsin School of Medicine, Madison, Wisconsin 53706'], 'affiliations': [{'raw_affiliation_string': 'From the Department of Physiology, University of Wisconsin School of Medicine, Madison, Wisconsin 53706', 'institution_ids': ['https://openalex.org/I135310074']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5075074631', 'display_name': 'Edwin R. Chapman', 'orcid': 'https://orcid.org/0000-0001-9787-8140'}, 'institutions': [{'id': 'https://openalex.org/I135310074', 'display_name': 'University of Wisconsin–Madison', 'ror': 'https://ror.org/01y2jtd41', 'country_code': 'US', 'type': 'education', 'lineage': ['https://openalex.org/I135310074']}], 'countries': ['US'], 'is_corresponding': False, 'raw_author_name': 'Edwin R. Chapman', 'raw_affiliation_strings': ['From the Department of Physiology, University of Wisconsin School of Medicine, Madison, Wisconsin 53706'], 'affiliations': [{'raw_affiliation_string': 'From the Department of Physiology, University of Wisconsin School of Medicine, Madison, Wisconsin 53706', 'institution_ids': ['https://openalex.org/I135310074']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'apc_paid': {'value': 2500, 'currency': 'USD', 'value_usd': 2500, 'provenance': 'doaj'}, 'fwci': 4.033, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 89, 'citation_normalized_percentile': {'value': 0.905128, 'is_in_top_1_percent': False, 'is_in_top_10_percent': True}, 'cited_by_percentile_year': {'min': 95, 'max': 96}, 'biblio': {'volume': '275', 'issue': '33', 'first_page': '25427', 'last_page': '25435'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T10617', 'display_name': 'Cellular transport and secretion', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T10617', 'display_name': 'Cellular transport and secretion', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10407', 'display_name': 'Lipid Membrane Structure and Behavior', 'score': 0.9997, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10077', 'display_name': 'Neuroscience and Neuropharmacology Research', 'score': 0.9981, 'subfield': {'id': 'https://openalex.org/subfields/2804', 'display_name': 'Cellular and Molecular Neuroscience'}, 'field': {'id': 'https://openalex.org/fields/28', 'display_name': 'Neuroscience'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/synaptotagmin-1', 'display_name': 'Synaptotagmin 1', 'score': 0.9536481}, {'id': 'https://openalex.org/keywords/synaptotagmin-i', 'display_name': 'Synaptotagmin I', 'score': 0.67583835}, {'id': 'https://openalex.org/keywords/vesicle-fusion', 'display_name': 'Vesicle fusion', 'score': 0.589583}, {'id': 'https://openalex.org/keywords/linker', 'display_name': 'Linker', 'score': 0.4511461}], 'concepts': [{'id': 'https://openalex.org/C86473631', 'wikidata': 'https://www.wikidata.org/wiki/Q18031809', 'display_name': 'Synaptotagmin 1', 'level': 5, 'score': 0.9536481}, {'id': 'https://openalex.org/C175969161', 'wikidata': 'https://www.wikidata.org/wiki/Q323426', 'display_name': 'Exocytosis', 'level': 3, 'score': 0.74050856}, {'id': 'https://openalex.org/C130316041', 'wikidata': 'https://www.wikidata.org/wiki/Q189206', 'display_name': 'Vesicle', 'level': 3, 'score': 0.6827971}, {'id': 'https://openalex.org/C2909695770', 'wikidata': 'https://www.wikidata.org/wiki/Q426764', 'display_name': 'Synaptotagmin I', 'level': 5, 'score': 0.67583835}, {'id': 'https://openalex.org/C148785051', 'wikidata': 'https://www.wikidata.org/wiki/Q671136', 'display_name': 'Synaptic vesicle', 'level': 4, 'score': 0.6349099}, {'id': 'https://openalex.org/C196330066', 'wikidata': 'https://www.wikidata.org/wiki/Q7923216', 'display_name': 'Vesicle fusion', 'level': 5, 'score': 0.589583}, {'id': 'https://openalex.org/C12554922', 'wikidata': 'https://www.wikidata.org/wiki/Q7100', 'display_name': 'Biophysics', 'level': 1, 'score': 0.58180207}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.52941334}, {'id': 'https://openalex.org/C41625074', 'wikidata': 'https://www.wikidata.org/wiki/Q176088', 'display_name': 'Membrane', 'level': 2, 'score': 0.46878898}, {'id': 'https://openalex.org/C2780557392', 'wikidata': 'https://www.wikidata.org/wiki/Q523796', 'display_name': 'Linker', 'level': 2, 'score': 0.4511461}, {'id': 'https://openalex.org/C39944091', 'wikidata': 'https://www.wikidata.org/wiki/Q423279', 'display_name': 'Lipid bilayer', 'level': 3, 'score': 0.44328246}, {'id': 'https://openalex.org/C95444343', 'wikidata': 'https://www.wikidata.org/wiki/Q7141', 'display_name': 'Cell biology', 'level': 1, 'score': 0.40525383}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.2867554}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.27139425}, {'id': 'https://openalex.org/C41008148', 'wikidata': 'https://www.wikidata.org/wiki/Q21198', 'display_name': 'Computer science', 'level': 0, 'score': 0.0}, {'id': 'https://openalex.org/C111919701', 'wikidata': 'https://www.wikidata.org/wiki/Q9135', 'display_name': 'Operating system', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D002135', 'descriptor_name': 'Calcium-Binding Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D002462', 'descriptor_name': 'Cell Membrane', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D008562', 'descriptor_name': 'Membrane Glycoproteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D008562', 'descriptor_name': 'Membrane Glycoproteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D009419', 'descriptor_name': 'Nerve Tissue Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': True}, {'descriptor_ui': 'D009419', 'descriptor_name': 'Nerve Tissue Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': True}, {'descriptor_ui': 'D033921', 'descriptor_name': 'Vesicular Transport Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': True}, {'descriptor_ui': 'D020106', 'descriptor_name': 'Acrylamide', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D020106', 'descriptor_name': 'Acrylamide', 'qualifier_ui': 'Q000494', 'qualifier_name': 'pharmacology', 'is_major_topic': False}, {'descriptor_ui': 'D000818', 'descriptor_name': 'Animals', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D000911', 'descriptor_name': 'Antibodies, Monoclonal', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D000911', 'descriptor_name': 'Antibodies, Monoclonal', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D002118', 'descriptor_name': 'Calcium', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002118', 'descriptor_name': 'Calcium', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D002462', 'descriptor_name': 'Cell Membrane', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003593', 'descriptor_name': 'Cytoplasm', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003593', 'descriptor_name': 'Cytoplasm', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D004305', 'descriptor_name': 'Dose-Response Relationship, Drug', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D004926', 'descriptor_name': 'Escherichia coli', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D005982', 'descriptor_name': 'Glutathione Transferase', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005982', 'descriptor_name': 'Glutathione Transferase', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D007700', 'descriptor_name': 'Kinetics', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008051', 'descriptor_name': 'Lipid Bilayers', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008051', 'descriptor_name': 'Lipid Bilayers', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D008081', 'descriptor_name': 'Liposomes', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008081', 'descriptor_name': 'Liposomes', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D008562', 'descriptor_name': 'Membrane Glycoproteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008565', 'descriptor_name': 'Membrane Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D008565', 'descriptor_name': 'Membrane Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D008954', 'descriptor_name': 'Models, Biological', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D016297', 'descriptor_name': 'Mutagenesis, Site-Directed', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009419', 'descriptor_name': 'Nerve Tissue Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011233', 'descriptor_name': 'Precipitin Tests', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011485', 'descriptor_name': 'Protein Binding', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017433', 'descriptor_name': 'Protein Structure, Secondary', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D017434', 'descriptor_name': 'Protein Structure, Tertiary', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D051381', 'descriptor_name': 'Rats', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000737', 'qualifier_name': 'chemistry', 'is_major_topic': False}, {'descriptor_ui': 'D011993', 'descriptor_name': 'Recombinant Fusion Proteins', 'qualifier_ui': 'Q000378', 'qualifier_name': 'metabolism', 'is_major_topic': False}, {'descriptor_ui': 'D050600', 'descriptor_name': 'SNARE Proteins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013050', 'descriptor_name': 'Spectrometry, Fluorescence', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D050863', 'descriptor_name': 'Synaptotagmin I', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D050857', 'descriptor_name': 'Synaptotagmins', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D013997', 'descriptor_name': 'Time Factors', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m906729199', 'pdf_url': 'http://www.jbc.org/article/S0021925819621046/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/10840045', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': {'is_oa': True, 'landing_page_url': 'https://doi.org/10.1074/jbc.m906729199', 'pdf_url': 'http://www.jbc.org/article/S0021925819621046/pdf', 'source': {'id': 'https://openalex.org/S140251998', 'display_name': 'Journal of Biological Chemistry', 'issn_l': '0021-9258', 'issn': ['0021-9258', '1067-8816', '1083-351X'], 'is_oa': True, 'is_in_doaj': True, 'is_core': True, 'host_organization': 'https://openalex.org/P4310320990', 'host_organization_name': 'Elsevier BV', 'host_organization_lineage': ['https://openalex.org/P4310320990'], 'host_organization_lineage_names': ['Elsevier BV'], 'type': 'journal'}, 'license': 'cc-by', 'license_id': 'https://openalex.org/licenses/cc-by', 'version': 'publishedVersion', 'is_accepted': True, 'is_published': True}, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 46, 'referenced_works': ['https://openalex.org/W135922689', 'https://openalex.org/W1529696685', 'https://openalex.org/W1530469978', 'https://openalex.org/W1555714096', 'https://openalex.org/W1568294354', 'https://openalex.org/W1584365695', 'https://openalex.org/W1591133722', 'https://openalex.org/W185955435', 'https://openalex.org/W1967817609', 'https://openalex.org/W1969359430', 'https://openalex.org/W1970930136', 'https://openalex.org/W1976283234', 'https://openalex.org/W1982630258', 'https://openalex.org/W1990220309', 'https://openalex.org/W1996862455', 'https://openalex.org/W2002162526', 'https://openalex.org/W2005593816', 'https://openalex.org/W2007982098', 'https://openalex.org/W2014181207', 'https://openalex.org/W2018757497', 'https://openalex.org/W2020593055', 'https://openalex.org/W2027775552', 'https://openalex.org/W2028231353', 'https://openalex.org/W2040585305', 'https://openalex.org/W2048015603', 'https://openalex.org/W2049308967', 'https://openalex.org/W2050367367', 'https://openalex.org/W2052373139', 'https://openalex.org/W2061491052', 'https://openalex.org/W2065359945', 'https://openalex.org/W2071740908', 'https://openalex.org/W2075183645', 'https://openalex.org/W2076924373', 'https://openalex.org/W2078230520', 'https://openalex.org/W2080528351', 'https://openalex.org/W2083258595', 'https://openalex.org/W2085680244', 'https://openalex.org/W2086036400', 'https://openalex.org/W2093687422', 'https://openalex.org/W2099559490', 'https://openalex.org/W2110533122', 'https://openalex.org/W2125384218', 'https://openalex.org/W2154472809', 'https://openalex.org/W2165302477', 'https://openalex.org/W2171279562', 'https://openalex.org/W2314093943'], 'related_works': ['https://openalex.org/W2394680308', 'https://openalex.org/W2265219157', 'https://openalex.org/W2134354906', 'https://openalex.org/W2082158539', 'https://openalex.org/W2046505029', 'https://openalex.org/W2046363062', 'https://openalex.org/W2044512158', 'https://openalex.org/W2039464724', 'https://openalex.org/W2038870054', 'https://openalex.org/W1506047834'], 'abstract_inverted_index': {'The': [0, 189, 444, 661, 853, 1362, 1564, 2367, 2728, 2769, 2790, 2839, 2862, 3485, 3948, 4233], 'synaptic': [1, 167, 190, 356, 431, 515, 1076, 1318, 4189], 'vesicle': [2, 21, 78, 191, 210, 267, 516, 605, 1077, 1319, 4190], 'protein': [3, 182, 192, 371, 408, 418, 517, 1078, 1359, 1877, 2358, 2630, 2638, 2656, 3053, 3976, 4223], 'synaptotagmin': [4, 58, 89, 159, 193, 247, 278, 348, 382, 387, 396, 400, 602, 863, 959, 1315, 1347, 1417, 1471, 1479, 1568, 1748, 2244, 2308, 2414, 2441, 2454, 2464, 3038, 3366, 3418, 3468, 3528, 3614, 3683, 3916, 3936, 3965, 3995, 4128, 4184, 4200], 'I': [5, 194, 506, 1480, 1749, 1930, 2028, 2465], 'has': [6, 195, 519, 609, 1133, 3938], 'been': [7, 196, 520, 671, 1134, 3939], 'proposed': [8, 197, 521, 1135], 'to': [9, 198, 509, 522, 533, 657, 699, 780, 952, 960, 1136, 1270, 1288, 1305, 1367, 1381, 1399, 1468, 1777, 2099, 2216, 2226, 2457, 2584, 2732, 2785, 2797, 2868, 2942, 3031, 3136, 3367, 3469, 3685, 3729, 3735, 3784, 3798, 3889, 3903, 3926, 3931, 3954, 4005, 4076, 4106, 4118, 4281, 4316, 4333], 'serve': [10, 199, 3173], 'as': [11, 143, 200, 332, 510, 524, 1138, 1304, 1588, 1601, 1649, 1675, 1715, 1811, 1827, 1847, 1893, 1968, 2120, 2273, 2767, 3174, 3852, 3854, 3863, 3893, 4122, 4170, 4194, 4214, 4220, 4228], 'a': [12, 26, 86, 144, 152, 201, 215, 275, 333, 341, 513, 525, 610, 616, 678, 916, 1069, 1142, 1275, 1369, 1374, 1394, 1413, 1795, 1842, 1875, 1880, 1953, 1960, 2383, 2445, 2460, 2610, 2621, 2710, 2721, 2842, 2849, 3690, 3986, 4023, 4053, 4058, 4067, 4090, 4103, 4195, 4203, 4221, 4270, 4304, 4310, 4329], 'Ca2+': [13, 202, 526, 534, 701, 782, 1268, 1799, 2145, 2163, 2169, 2335, 2760, 3142, 3176, 3241, 3315, 3343, 3622, 3641, 3793, 4133], 'sensor': [14, 203, 527], 'for': [15, 176, 204, 365, 1832, 2190, 2207, 2336, 2351, 2364, 2385, 2424, 2543, 2774, 2828, 2848, 2948, 3148, 3358, 3531, 3654, 3869, 4140, 4165], 'rapid': [16, 107, 177, 205, 296, 366, 2682], 'exocytosis.': [17, 206], 'Synaptotagmin': [18, 207, 505, 1200], 'spans': [19, 208, 603], 'the': [20, 44, 51, 68, 77, 92, 112, 118, 133, 136, 164, 181, 184, 209, 233, 240, 257, 266, 281, 301, 307, 322, 325, 353, 370, 373, 392, 438, 452, 604, 654, 658, 667, 692, 695, 859, 912, 924, 929, 961, 1075, 1130, 1139, 1203, 1279, 1282, 1290, 1296, 1302, 1308, 1311, 1317, 1329, 1350, 1358, 1383, 1406, 1421, 1425, 1430, 1437, 1442, 1462, 1779, 1883, 2038, 2100, 2130, 2202, 2220, 2326, 2344, 2399, 2408, 2411, 2419, 2580, 2673, 2678, 2748, 2787, 2798, 2811, 2815, 2825, 2834, 2837, 2915, 2918, 2922, 2936, 2939, 2943, 2952, 2956, 2960, 2970, 2975, 2986, 2989, 2992, 3005, 3008, 3034, 3042, 3047, 3052, 3138, 3141, 3155, 3208, 3239, 3259, 3272, 3312, 3318, 3362, 3410, 3413, 3455, 3464, 3472, 3475, 3621, 3625, 3633, 3638, 3644, 3664, 3686, 3724, 3730, 3740, 3749, 3764, 3785, 3792, 3799, 3804, 3808, 3837, 3847, 3855, 3875, 3890, 3910, 3920, 3960, 3991, 4006, 4013, 4094, 4098, 4119, 4123, 4132, 4179, 4188, 4208, 4260, 4283, 4300, 4317, 4334, 4339, 4346, 4354], 'membrane': [22, 62, 137, 185, 211, 251, 326, 374, 436, 606, 963, 1144, 1320, 1332, 3962, 4181, 4206], 'once': [23, 212, 607], 'and': [24, 37, 54, 94, 139, 213, 226, 243, 283, 328, 391, 608, 615, 628, 663, 764, 772, 1016, 1066, 1207, 1264, 1295, 1354, 1393, 1402, 1453, 1529, 1559, 1578, 1593, 1673, 1707, 1750, 1760, 1773, 1800, 1807, 1825, 1835, 1938, 1945, 2006, 2023, 2147, 2156, 2205, 2213, 2222, 2228, 2243, 2256, 2354, 2373, 2377, 2527, 2586, 2614, 2631, 2641, 2664, 2704, 2741, 2777, 2780, 2808, 2817, 2831, 2966, 2978, 2983, 3135, 3146, 3154, 3171, 3399, 3460, 3483, 3629, 3631, 3680, 3780, 3858, 3867, 3877, 3996, 4009, 4027, 4041, 4046, 4089, 4114, 4138, 4162, 4224], 'possesses': [25, 214, 3039], 'cytoplasmic': [27, 216, 397, 619, 913, 1565, 3049, 3261, 3363, 3465, 3848], 'domain': [28, 96, 141, 156, 217, 285, 330, 345, 380, 385, 390, 394, 398, 614, 669, 676, 693, 856, 914, 1284, 1313, 1356, 1364, 1464, 1566, 1575, 1580, 3036, 3050, 3210, 3262, 3275, 3320, 3364, 3416, 3466, 3627, 3646, 3849, 3988, 4020, 4126], 'largely': [29, 218], 'comprised': [30, 219], 'of': [31, 47, 88, 121, 127, 166, 172, 180, 220, 236, 277, 310, 316, 355, 361, 369, 381, 386, 395, 399, 419, 430, 440, 666, 691, 697, 862, 928, 958, 1141, 1244, 1281, 1310, 1314, 1346, 1357, 1385, 1408, 1416, 1424, 1432, 1439, 1441, 1445, 1461, 1567, 1632, 1699, 1702, 1710, 1747, 1765, 1927, 2044, 2167, 2219, 2266, 2328, 2357, 2388, 2410, 2421, 2440, 2463, 2519, 2549, 2579, 2672, 2726, 2750, 2792, 2819, 2836, 2855, 2917, 2955, 2974, 2991, 3007, 3037, 3041, 3046, 3051, 3140, 3236, 3238, 3258, 3263, 3271, 3309, 3311, 3361, 3365, 3412, 3417, 3425, 3457, 3463, 3467, 3474, 3480, 3527, 3637, 3666, 3697, 3739, 3767, 3787, 3791, 3836, 3842, 3846, 3879, 3909, 3915, 3950, 3994, 4018, 4097, 4127, 4135, 4235, 4242, 4259, 4266, 4299, 4303, 4336], 'two': [32, 221, 623, 698, 1752, 2993], 'C2': [33, 222, 379, 384, 389, 624, 655, 855, 949, 3525], 'domains': [34, 223, 656, 950, 3526], 'designated': [35, 224, 626, 1129, 4192, 4212], 'C2A': [36, 74, 95, 140, 225, 263, 284, 329, 627, 668, 1283, 1312, 1355, 1363, 1386, 1463, 1574, 3035, 3145, 3179, 3274, 3415, 3626, 3805, 3833, 3857, 3886, 3921, 3957, 3973, 3987, 4014, 4125, 4176], 'C2B.': [38, 227], 'We': [39, 228, 3448, 3983], 'have': [40, 229, 670, 1341, 3449, 3825, 4064], 'determined': [41, 230, 2141], 'how': [42, 231], 'deep': [43, 232, 2979], 'Ca2+-binding': [45, 234, 514, 769, 1443], 'loops': [46, 235, 685, 770, 1444], 'Ca2+·C2A': [48, 237, 1326], 'penetrate': [49, 238, 774, 1208], 'into': [50, 76, 98, 149, 239, 265, 287, 338, 775, 915, 1209, 1360, 1387, 1817, 3941, 4071, 4226, 4291], 'lipid': [52, 241, 776, 1297, 2634, 2961, 4072], 'bilayer': [53, 242, 2923, 2940], 'report': [55, 244, 3349], 'mutations': [56, 245, 1776, 3619], 'in': [57, 111, 246, 300, 531, 673, 778, 1277, 1389, 1585, 1798, 1874, 2025, 2193, 2201, 2309, 2325, 2418, 2427, 2453, 2513, 2546, 2553, 2567, 2738, 2743, 2747, 2814, 2988, 3144, 3207, 3232, 3317, 3517, 3620, 3632, 3663, 3672, 3689, 3733, 3774, 3874, 3883, 3901, 3963, 4031, 4066, 4074, 4083, 4086, 4093, 4111, 4167, 4182, 4250, 4254, 4322, 4338], 'that': [59, 90, 103, 132, 158, 248, 279, 292, 321, 347, 518, 528, 621, 686, 768, 921, 1348, 1450, 1791, 1878, 3161, 3335, 3351, 3394, 3409, 3795, 3822, 3832, 3907, 3937, 3989, 4157, 4286, 4328], 'can': [60, 249, 1201, 1456, 3402, 3811], 'uncouple': [61, 250, 3812], 'penetration': [63, 252, 1384, 1440, 4081], 'from': [64, 253, 442, 688, 2011, 2107, 2133, 2782, 2921, 2938, 3816], 'Ca2+-triggered': [65, 178, 254, 367, 428, 1451, 2458, 3150, 3253, 3326, 3336, 3359, 3395, 3813, 3817, 4108], 'interactions': [66, 105, 255, 294, 1239, 1434, 1455, 1472, 3151, 3396, 3534, 3699, 3815, 3824, 3934, 4110], 'with': [67, 256, 433, 1074, 1285, 1316, 1328, 1771, 1951, 2306, 2346, 2379, 2535, 2637, 2646, 3152, 3247, 3269, 3397, 3400, 3419, 3535, 3656, 3694, 3959, 3975, 4039, 4052, 4263], 'SNARE': [69, 258, 1131, 1205, 1696, 2268, 2302, 2432, 3156, 3477, 3489, 3658, 3687, 3731, 3757, 3800], 'complex.': [70, 259, 3157], 'To': [71, 260, 1336, 2017, 2450, 3829, 4173], 'determine': [72, 261, 3955, 4174], 'whether': [73, 262, 1306, 1325, 3033, 3956, 3972, 4175, 4199], 'penetrates': [75, 264, 4070], '("cis")': [79, 268], 'or': [80, 269, 918, 926, 1324, 1959, 2332, 2558, 2574, 2757, 2999, 3249, 3668, 3971, 4198, 4276, 4309], 'plasma': [81, 270, 435, 962, 1331, 4210], '("trans")': [82, 271], 'membrane,': [83, 272], 'we': [84, 273, 1340, 1411, 1428, 1448, 3611, 3923, 4063, 4352], 'reconstituted': [85, 274, 1342, 3940, 4225], 'fragment': [87, 276, 1345], 'includes': [91, 280, 3990], 'membrane-spanning': [93, 282], '(C2A-TMR)': [97, 286], 'proteoliposomes.': [99, 288, 1361, 4264, 4292], 'Kinetics': [100, 289], 'experiments': [101, 290, 1410, 2258, 2425, 2684, 3952], 'revealed': [102, 291], 'cis': [104, 293, 4196], 'are': [106, 295, 2810, 2969, 3204, 3267, 3529, 3772], '(≤500': [108, 297], 'μs).': [109, 298], 'Binding': [110, 299], 'trans': [113, 122, 302, 311, 1403], 'mode': [114, 171, 303, 360, 1415], 'was': [115, 304, 1365, 1397, 1646, 1671, 1769, 1788, 1839, 1888, 1922, 2105, 2140, 2270, 2340, 2442, 2511, 2533, 2668, 2707, 2730, 2794, 2865, 3355, 3421, 3652, 3742, 4050, 4218, 4238, 4288], 'distinguished': [116, 305], 'by': [117, 306, 1435, 1553, 1815, 2036, 2185, 2249, 2342, 2375, 2670, 2677, 2929, 2959, 3245, 3342, 3423, 3453, 3744, 3919, 4240], 'slow': [119, 308], 'diffusion': [120, 309], 'target': [123, 312, 3978, 4205], 'vesicles.': [124, 168, 313, 357], 'Both': [125, 314, 948], 'modes': [126, 315], 'binding': [128, 317, 696, 781, 957, 2115, 2412, 2568, 3044, 3256, 3338, 3360, 3759, 3768, 3786, 3797, 3840, 3872, 3913, 4077, 4141], 'were': [129, 318, 1550, 1583, 1641, 1713, 1809, 1823, 1966, 2009, 2021, 2034, 2090, 2117, 2174, 2183, 2199, 2224, 2246, 2259, 2304, 2369, 2391, 2563, 2582, 2596, 2604, 2618, 2635, 2685, 2699, 2717, 2772, 3030, 3647, 3861, 4021, 4037, 4143], 'observed,': [130, 319], 'indicating': [131, 320, 3821, 4285], 'linker': [134, 323], 'between': [135, 324, 1293], 'anchor': [138, 327, 4008], 'functions': [142, 331], 'flexible': [145, 334, 684], 'tether.': [146, 335], 'C2A-TMR': [147, 336, 4113], 'assembled': [148, 337, 1204, 3487, 3657], 'oligomers': [150, 339], 'via': [151, 183, 340, 372, 1420, 1459, 1473, 3943], 'novel': [153, 170, 342, 359, 1414], 'N-terminal': [154, 343, 378, 613, 1344, 1422, 3945], 'oligomerization': [155, 179, 344, 368, 861, 1418], 'suggesting': [157, 346, 3393], 'may': [160, 174, 349, 363, 922, 1266], 'form': [161, 350, 1068, 4012, 4029], 'clusters': [162, 351], 'on': [163, 352, 451, 2531, 3325], 'surface': [165, 354], 'This': [169, 358, 675, 1127, 1299, 1886], 'clustering': [173, 362, 911], 'allow': [175, 364], 'distal': [186, 375], 'C2B': [187, 376, 1579, 3147, 3209, 3319, 3645, 3859, 3896], 'domain.': [188, 377, 660, 3806, 4015], 'C-terminal': [383, 618], 'first': [388, 3028], 'transmembrane': [393, 659, 3946, 3992, 4007], 'glutathioneS-transferase': [401], 'polyacrylamide': [402], 'gel': [403], 'electrophoresis': [404], 'soluble': [405, 1949, 2538], 'NSF': [406], 'attachment': [407], 'receptor': [409], 'phosphatidylserine': [410, 1997], 'phosphatidylcholine': [411, 1999], 'dithiothreitol': [412], 'fluorescence': [413, 763, 1833, 2060, 2602, 2650, 2676, 2788, 2812, 2972, 4087], 'resonance': [414, 2651], 'energy': [415, 2652], 'transfer': [416, 2653], 'synaptosomal-associated': [417], '25': [420], 'kDa': [421], 'synthetic': [422, 2001], 'dansyl-phosphatidylethanolamine': [423, 2002], '7-Br2-PC,': [424], '1-palmitoyl-2-stearoyl-(6,7)-dibromo-sn-glycero-3-phosphocholine': [425, 2004], '12-Br2-PC,': [426], '1-palmitoyl-2-stearoyl-(11,12)-dibromo-sn-glycero-3-phosphocholine': [427, 2007], 'fusion': [429, 930, 1145, 1590, 1813, 1876, 3865], 'vesicles': [432, 3979], 'presynaptic': [434, 1330, 4209], 'mediates': [437, 858, 3834], 'release': [439, 445, 530], 'neurotransmitters': [441], 'neurons.': [443], 'process': [446, 4331], 'is': [447, 512, 2824, 2833, 2927, 2951, 2985, 3011, 3340, 3515, 3917, 3953, 3966, 4159, 4163, 4185], 'extremely': [448], 'fast,': [449], 'occurring': [450, 1754, 1781], 'sub-millisecond': [453], 'time': [454], 'scale': [455], '(1Llinas': [456], 'R.': [457, 476, 637, 871, 999, 1248, 1488, 1519, 1560, 1610, 1654, 1684, 1856, 1902, 2250, 2473, 3057, 3093, 3218, 3376, 3570, 3707], 'Steinberg': [458], 'I.Z.': [459], 'Walton': [460], 'K.': [461, 2074], 'Biophys.': [462, 2080], 'J.': [463, 732, 743, 751, 753, 787, 806, 837, 872, 894, 969, 1000, 1049, 1118, 1215, 1502, 1538, 1611, 1655, 1686, 1720, 1857, 1903, 1973, 2278, 2488, 2898, 3058, 3074, 3094, 3280, 3295, 3377, 3506, 3540, 3571, 3595, 3708], '1981;': [464], '33:': [465], '323-352Abstract': [466], 'Full': [467, 554, 593, 717, 793, 795, 819, 821, 843, 845, 878, 880, 900, 902, 982, 984, 1006, 1008, 1055, 1057, 1190, 1192, 1228, 1230, 1544, 1617, 1619, 1661, 1733, 1735, 1863, 1865, 1909, 1911, 1986, 1988, 2291, 2293, 2501, 2503, 3064, 3080, 3100, 3102, 3194, 3286, 3383, 3385, 3553, 3555, 3577, 3579, 3601, 3603, 3714, 3716], 'Text': [468, 555, 594, 718, 794, 796, 820, 822, 844, 846, 879, 881, 901, 903, 983, 985, 1007, 1009, 1056, 1058, 1191, 1193, 1229, 1231, 1545, 1618, 1620, 1662, 1734, 1736, 1864, 1866, 1910, 1912, 1987, 1989, 2292, 2294, 2502, 2504, 3065, 3081, 3101, 3103, 3195, 3287, 3384, 3386, 3554, 3556, 3578, 3580, 3602, 3604, 3715, 3717], 'PDF': [469, 556, 595, 719, 797, 823, 847, 882, 904, 986, 1010, 1059, 1194, 1232, 1546, 1621, 1663, 1737, 1867, 1913, 1990, 2295, 2505, 3066, 3082, 3104, 3196, 3288, 3387, 3557, 3581, 3605, 3718], 'PubMed': [470, 487, 500, 557, 580, 596, 644, 720, 737, 757, 798, 824, 848, 883, 905, 943, 987, 1011, 1036, 1060, 1098, 1122, 1165, 1195, 1233, 1259, 1495, 1524, 1547, 1622, 1664, 1690, 1738, 1868, 1914, 1991, 2085, 2296, 2480, 2506, 2879, 2892, 2909, 3022, 3067, 3083, 3105, 3130, 3197, 3225, 3289, 3388, 3443, 3510, 3558, 3582, 3606, 3719], 'Scopus': [471, 488, 501, 558, 581, 597, 645, 721, 738, 758, 799, 825, 849, 884, 906, 944, 988, 1012, 1037, 1061, 1099, 1123, 1166, 1196, 1234, 1260, 1496, 1525, 1623, 1691, 1739, 1869, 1915, 1992, 2086, 2297, 2481, 2507, 2880, 2893, 2910, 3023, 3106, 3131, 3198, 3226, 3302, 3389, 3444, 3511, 3559, 3583, 3607, 3720], '(504)': [472], 'Google': [473, 490, 503, 560, 583, 599, 647, 723, 740, 760, 801, 827, 851, 886, 908, 946, 990, 1014, 1039, 1063, 1101, 1125, 1168, 1198, 1236, 1262, 1498, 1527, 1548, 1625, 1665, 1693, 1741, 1871, 1917, 1994, 2088, 2299, 2483, 2509, 2882, 2895, 2912, 3025, 3068, 3084, 3108, 3133, 3200, 3228, 3290, 3304, 3391, 3446, 3513, 3561, 3585, 3609, 3722], 'Scholar,': [474, 491, 561, 584, 724, 741, 802, 828, 887, 991, 1040, 1102, 1169, 2484, 2883, 2896, 3069, 3085, 3109, 3291, 3562, 3586], '2Heidelberger': [475], 'Heinemann': [477, 1249], 'C.': [478, 543, 1250, 1555, 3428], 'Neher': [479, 1251], 'E.': [480, 1252, 1506, 2874, 2887, 2904], 'Matthews': [481, 1253], 'G.': [482, 1019, 1021, 1254, 3111], 'Nature.': [483, 496, 640, 1094, 1161, 1255, 1491, 1520, 2476, 3221, 3439], '1994;': [484, 551, 577, 590, 1119, 1256, 1658, 3061, 3507], '371:': [485, 1257], '513-515Crossref': [486, 1258], '(627)': [489, 1261], '3Sabatini': [492], 'B.L.': [493], 'Regehr': [494], 'W.G.': [495], '1996;': [497, 875, 897, 1003, 1614, 1860, 1906, 3127, 3574], '384:': [498], '170-172Crossref': [499], '(323)': [502, 3107, 3390, 3721], 'Scholar).': [504, 600, 648, 761, 852, 947, 1064, 1126, 1199, 1237, 1626, 1666, 1694, 1742, 1918, 1995, 2300, 2861, 2913, 3026, 3201, 3229, 3305, 3447], '(hereafter': [507], 'referred': [508], 'synaptotagmin)': [511], 'function': [523, 1137], 'triggers': [529], 'response': [532, 779, 2779, 3734, 3902, 4075], 'influx': [535, 1269], '(4Geppert': [536], 'M.': [537, 565, 567, 631, 933, 1085, 1152, 1179, 1482, 2467, 3212], 'Goda': [538], 'Y.': [539, 891, 1112, 3500], 'Hammer': [540], 'R.E.': [541], 'Li': [542], 'Rosahl': [544], 'T.W.': [545], 'Stevens': [546], 'C.F.': [547], 'Südhof': [548, 638, 709, 729, 748, 892, 1113, 1489, 1514, 1556, 2474, 3072, 3186, 3219, 3278, 3296, 3437, 3501], 'T.C.': [549, 639, 710, 730, 749, 893, 1114, 1490, 1515, 2475, 3073, 3187, 3220, 3279, 3297, 3438, 3502], 'Cell.': [550, 713, 1186, 3190], '79:': [552], '717-727Abstract': [553], '(1239)': [559], '5Littleton': [562], 'J.T.': [563], 'Stern': [564], 'Perin': [566], 'Bellen': [568], 'H.J.': [569], 'Proc.': [570, 1026, 3120], 'Natl.': [571, 1027, 3121], 'Acad.': [572, 1028, 3122], 'Sci.': [573, 1029, 3123], 'U.': [574, 1030, 3124], 'S.': [575, 867, 889, 995, 1031, 1089, 1108, 1156, 1510, 1606, 1852, 1898, 2900, 3091, 3125, 3374, 3496, 3566, 3705], 'A.': [576, 586, 1032, 2885, 3126], '91:': [578], '10888-10892Crossref': [579], '(251)': [582], '6DiAntonio': [585], 'Schwarz': [587], 'T.L.': [588], 'Neuron.': [589, 815, 978, 1224, 1729, 1982, 2287, 2497, 3549], '12:': [591], '909-920Abstract': [592], '(214)': [598], 'Structurally,': [601], 'short': [611], 'intravesicular': [612], 'large': [617, 2062], 'region': [620, 1423, 3010], 'contains': [622, 1751, 1794, 1879], 'domains,': [625, 3860], 'C2B1': [629], '(7Perin': [630, 1481, 2466, 3211], 'Fried': [632, 1483, 2468, 3213], 'V.A.': [633, 1484, 2469, 3214], 'Mignery': [634, 1485, 2470, 3215], 'G.A.': [635, 1486, 2471, 3216], 'Jahn': [636, 870, 998, 1487, 1518, 1609, 1653, 1683, 1855, 1901, 2251, 2472, 3056, 3092, 3217, 3375, 3569, 3706], '1990;': [641, 1492, 2477, 3222], '345:': [642, 1493, 2478, 3223], '260-263Crossref': [643, 1494, 2479, 3224], '(666)': [646, 1497, 2482, 3227], 'A': [649, 1762, 1784, 1844, 3347], 'charged': [650], '"linker"': [651], 'segment': [652, 3993], 'connects': [653], 'structure': [662, 920], 'biochemical': [664], 'properties': [665], 'studied': [672], 'detail.': [674], 'forms': [677, 3462], 'compact': [679], 'eight-stranded': [680], 'β-sandwich': [681], 'structure.': [682], 'Three': [683], 'protrude': [687], 'one': [689], 'end': [690], 'mediate': [694, 953], 'three': [700, 2371, 2565, 3613], 'ions': [702], '(8Sutton': [703, 3180], 'R.B.': [704, 3181], 'Davletov': [705, 3182], 'B.A.': [706, 3071, 3183, 3277], 'Berghuis': [707, 3184], 'A.M.': [708, 3185], 'Sprang': [711, 3188], 'S.R.': [712, 3189], '1995;': [714, 940, 1687, 3097, 3191, 3380, 3440, 3711], '80:': [715, 3192], '929-938Abstract': [716, 3193], '(618)': [722, 3199], '9Shao': [725], 'X.': [726, 3293], 'Fernandez': [727], 'I.': [728], 'Rizo': [731, 750, 3294], 'Biochemistry.': [733, 2875, 2888, 2905, 3018, 3298], '1998;': [734, 754, 790, 840, 1187, 3299], '37:': [735], '16106-16115Crossref': [736], '(206)': [739], '10Ubach': [742], 'Zhang': [744, 3431], 'X.Y.': [745], 'Shao': [746], 'X.G.': [747], 'EMBO': [752, 1117, 1685, 3505], '17:': [755], '3921-3930Crossref': [756], '(254)': [759], 'Recent': [762], 'NMR': [765], 'studies': [766, 1834, 3159, 3270], 'demonstrated': [767], '1': [771, 1955, 2333, 2365, 2536, 2559, 2575, 2740, 2755, 2758, 2804, 2965, 3330, 3674, 4267], '3': [773, 1803, 1962, 4252], 'bilayers': [777, 4073], '(11Chapman': [783], 'E.R.': [784, 814, 834, 865, 977, 993, 1223, 1504, 1604, 1652, 1682, 1728, 1850, 1896, 1981, 2286, 2496, 3055, 3087, 3370, 3548, 3564, 3701], 'Davis': [785], 'A.F.': [786, 804, 967, 1213, 1718, 1971, 2276, 2486, 3538], 'Biol.': [788, 838, 873, 895, 939, 1001, 1050, 1539, 1612, 1656, 1858, 1904, 3059, 3075, 3095, 3281, 3378, 3572, 3596, 3709], 'Chem.': [789, 839, 874, 896, 1002, 1051, 1540, 1613, 1657, 1859, 1905, 3060, 3076, 3096, 3282, 3379, 3573, 3597, 3710], '273:': [791, 841], '13995-14001Abstract': [792], '(221)': [800], '12Davis': [803, 2485], 'Bai': [805, 968, 1214, 1719, 1972, 2277, 2487, 3539], 'Fasshauer': [807, 970, 1216, 1721, 1974, 2279, 2489, 3541], 'D.': [808, 971, 1217, 1722, 1975, 2280, 2490, 3542], 'Wolowick': [809, 972, 1218, 1723, 1976, 2281, 2491, 3543], 'M.J.': [810, 973, 1219, 1724, 1977, 2282, 2492, 3544], 'Lewis': [811, 974, 1220, 1725, 1978, 2283, 2493, 3545], 'J.L.': [812, 836, 975, 1221, 1726, 1979, 2284, 2494, 3546], 'Chapman': [813, 833, 976, 1222, 1503, 1681, 1727, 1980, 2285, 2495, 3547], '1999;': [816, 979, 1225, 1730, 1983, 2288, 2498, 3550], '24:': [817, 980, 1226, 1731, 1984, 2289, 2499, 3551], '363-376Abstract': [818, 981, 1227, 1732, 1985, 2290, 2500, 3552], '(218)': [826, 945, 989, 1235, 1740, 1993, 2298, 2508, 3560], '13Chae': [829], 'Y.K.': [830], 'Abildgaard': [831], 'F.': [832, 1181], 'Markley': [835], '25659-25663Abstract': [842], '(40)': [850], 'second': [854], '(C2B)': [857], 'Ca2+-dependent': [860, 956, 3691], '(14Chapman': [864, 1603, 1849, 1895], 'An': [866, 994, 1605, 1851, 1897, 3090, 3373, 3565, 3704], 'Edwardson': [868, 996, 1607, 1853, 1899, 3567], 'J.M.': [869, 997, 1608, 1854, 1900, 3568], '271:': [876, 898, 1004, 1615, 1861, 1907, 3575], '5844-5849Abstract': [877, 1005, 1616, 1862, 1908, 3576], '(185)': [885, 1013, 1624, 1870, 1916, 3584], '15Sugita': [888], 'Hata': [890, 1111, 3499], '1262-1265Abstract': [899], '(133)': [907], 'Scholar),': [909, 1499, 1528, 1872, 3392, 3610, 3723], 'potentially': [910], 'collar': [917], 'ring-like': [919], 'regulate': [923, 3796], 'opening': [925], 'dilation': [927], 'pore': [931], '(16Lindau': [932], 'Almers': [934], 'W.': [935], 'Curr.': [936], 'Opin.': [937], 'Cell': [938], '7:': [941], '509-517Crossref': [942], 'cooperate': [951], 'high': [954, 1070, 3532, 4271], 'affinity': [955, 1071, 3533], 'proteins': [964, 1814, 3866], 'syntaxin': [965, 1703, 3368, 3420], '(12Davis': [966, 1212, 1717, 1970, 2275, 3537], '14Chapman': [992, 3563], 'Scholar)': [1015, 1263, 1549, 2089, 2510, 3134, 3514], 'SNAP-25': [1017, 1067], '(17Schiavo': [1018], 'Stenbeck': [1020], 'Rothman': [1022, 1092, 1159, 1184, 3118], 'J.E.': [1023, 1093, 1160, 1185, 3119], 'Söllner': [1024, 1182, 3116], 'T.H.': [1025, 1183, 3117], '1997;': [1033, 2906], '94:': [1034], '997-1001Crossref': [1035], '(262)': [1038], '18Gerona': [1041, 3587], 'R.R.L.': [1042, 3588], 'Larsen': [1043, 3589], 'E.C.': [1044, 3590], 'Kowalchyk': [1045, 3591], 'J.A.': [1046, 1175, 3592], 'Martin': [1047, 3593], 'T.F.J.': [1048, 3594], '2000;': [1052, 3598], '275:': [1053, 3599], '6328-6336Abstract': [1054, 3600], '(199)': [1062, 3608], 'Syntaxin': [1065], 'ternary': [1072, 3476], 'complex': [1073, 1206, 3478, 3490, 3688, 3732, 3758, 3801, 3819], 'synaptobrevin': [1079, 1530, 1669, 1711, 2241], '(19Söllner': [1080, 1147], 'T.': [1081, 1104, 1110, 1148, 1171, 1508, 1554, 3492, 3498], 'Whiteheart': [1082, 1149], 'S.W.': [1083, 1150], 'Brunner': [1084, 1151], 'Erdjument-Bromage': [1086, 1153], 'H.': [1087, 1106, 1116, 1154, 1517, 3494, 3504], 'Geromanos': [1088, 1155], 'Tempst': [1090, 1157], 'P.': [1091, 1158, 1513, 1680], '1993;': [1095, 1162, 1521, 3077, 3283], '362:': [1096, 1163], '318-324Crossref': [1097, 1164], '(2670)': [1100, 1167], '20Hayashi': [1103], 'McMahon': [1105, 3493], 'Yamasaki': [1107, 1509, 3495], 'Binz': [1109, 1507, 3497], 'Niemann': [1115, 1516, 3503], '13:': [1120, 3508], '5051-5061Crossref': [1121, 3509], '(672)': [1124, 3512], 'heterotrimer,': [1128], 'complex,': [1132], 'core': [1140, 3473], 'conserved': [1143, 3206, 3640], '"machine"': [1146], '21Weber': [1170], 'Zemelman': [1172], 'B.V.': [1173], 'McNew': [1174], 'Westerman': [1176], 'B.': [1177, 3430], 'Gmachl': [1178], 'Parlati': [1180], '92:': [1188], '759-772Abstract': [1189], '(2049)': [1197], 'bind': [1202, 2404, 3899], 'membranes': [1210, 1286, 1388, 3153], 'simultaneously': [1211], 'These': [1238, 1272, 3202, 3265, 3332, 3770, 4154, 4325], 'occur': [1240], 'at': [1241, 1757, 1882, 2056, 2187, 2210, 2262, 2606, 2643, 2688, 2701], 'Ca2+concentrations': [1242], 'capable': [1243], 'eliciting': [1245], 'exocytosis': [1246], '(2Heidelberger': [1247], 'therefore': [1265, 3984], 'couple': [1267], 'fusion.': [1271], 'findings': [1273], 'prompt': [1274], 'model': [1276, 1300], 'which': [1278, 1377, 3964, 4032, 4062, 4183], 'interaction': [1280, 1309, 3411], 'serves': [1287], 'alter': [1289], 'physical': [1291], 'relationship': [1292], 'SNAREs': [1294, 3401, 3536], 'bilayer.': [1298], 'prompts': [1301], 'question': [1303], 'Ca2+triggers': [1307], '(a': [1321, 1333, 3968, 3980], '"cis"': [1322, 3969], 'interaction)': [1323, 3970, 4197, 4216], 'interacts': [1327, 3958, 3974], '"trans"': [1334, 3981], 'interaction).': [1335, 3982], 'address': [1337, 1469], 'this': [1338, 1766, 1792, 3451, 3745, 4019], 'issue': [1339, 3452], 'an': [1343, 2093, 2143, 2148, 2194, 2692, 3928, 4084], 'encodes': [1349, 1744], 'luminal,': [1351], 'transmembrane,': [1352], 'linker,': [1353], 'modified': [1366], 'contain': [1368], 'single': [1370, 1796, 4059], 'fluorescent': [1371], 'reporter': [1372, 1957, 1964, 2736, 3930], 'group,': [1373], 'tryptophan': [1375, 2919, 4054], '(Trp),': [1376], 'could': [1378], 'be': [1379, 1457, 3403], 'used': [1380, 1398, 2512], 'monitor': [1382, 4107], 'real-time': [1390], '(11,': [1391, 2122], '12),': [1392], 'kinetic': [1395], 'approach': [1396], 'discern': [1400], 'betweencis': [1401], 'interactions.': [1404], 'In': [1405, 1627, 2124, 2396, 3306, 3747], 'course': [1407], 'these': [1409, 3002, 3823, 3951], 'observed': [1412, 1449], 'mediated': [1419, 3918], 'protein.': [1426, 2032], 'Furthermore,': [1427, 4131], 'refined': [1429], 'geometry': [1431], 'C2A·membrane': [1433, 3933], 'measuring': [1436], 'depth': [1438, 2863, 2990], 'C2A.': [1446, 3346], 'Finally,': [1447], 'synaptotagmin·lipid': [1452, 3814], 'synaptotagmin·SNARE': [1454, 3818], 'uncoupled': [1458], 'mutagenesis': [1460, 1645], 'making': [1465], 'it': [1466], 'possible': [1467], 'discrete': [1470], 'genetic': [1474], 'approaches.': [1475], 'cDNAs': [1476], 'encoding': [1477], 'rat': [1478, 1668], 'SNAP-25A': [1500], '(22Blasi': [1501], 'Link': [1505], 'De': [1511], 'Camilli': [1512], '365:': [1522], '160-163Crossref': [1523], '(1070)': [1526], 'II': [1531, 1670, 2047, 2551], '(23Elferink': [1532], 'L.A.': [1533], 'Trimble': [1534], 'W.S.': [1535], 'Scheller': [1536, 1561], 'R.H.': [1537], '1989;': [1541], '264:': [1542], '11061-11064Abstract': [1543], 'kindly': [1551, 2247], 'provided': [1552, 2248], '(Dallas,': [1557], 'TX)': [1558], '(Stanford,': [1562], 'CA).': [1563], '(designated': [1569, 3850], 'C2AB;': [1570, 3895], 'amino': [1571, 3164, 3998], 'acids': [1572, 2523, 2541, 3999], '96–421),': [1573], '(residues': [1576, 1581], '96–265),': [1577], '248–421)': [1582], 'expressed': [1584, 1672, 1824, 1840], 'Escherichia': [1586], 'coli': [1587], 'GST': [1589, 1812, 3864], 'proteins,': [1591], 'immobilized': [1592, 2530, 3862], 'purified': [1594, 1674, 1826, 1889], 'using': [1595, 1841, 1890, 2092, 2142, 2176, 2231, 2444, 2589, 2598, 2609, 2620, 2691, 2709, 2720, 3660, 3935], 'glutathione-Sepharose': [1596], 'beads': [1597, 2532], '(Amersham': [1598, 1819, 2361], 'Pharmacia': [1599, 1820, 2109, 2362], 'Biotech)': [1600, 2363], 'described': [1602, 1650, 1676, 1716, 1828, 1848, 1894, 1969, 2121, 2274, 4171, 4229, 4349], 'addition,': [1628], 'point': [1629], 'mutant': [1630, 1763, 3461, 3635, 3651, 3682, 3726, 3751, 4028], 'versions': [1631, 4017], 'GST-C2AB': [1633], '(D172N,': [1634], 'D178N,': [1635], 'D230S,': [1636], 'D232N,': [1637], 'D238N,': [1638], 'D230N/D232N,': [1639], 'D309N/D363N/D365N/D371N),': [1640], 'also': [1642, 2392, 2435, 3727], 'prepared.': [1643], 'All': [1644, 2254], 'carried': [1647, 2118, 2260, 2271, 2686, 2718], 'out': [1648, 2119, 2261, 2272, 2687, 2719, 4345], '(24Chapman': [1651, 3054], '269:': [1659, 3062], '5735-5741Abstract': [1660, 3063], 'Full-length': [1667], '(25Edelmann': [1677], 'L.': [1678], 'Hanson': [1679, 3088, 3371, 3702], '14:': [1688], '224-231Crossref': [1689], '(395)': [1692], 'Midi': [1695], 'complexes,': [1697], 'composed': [1698, 3479], 'residues': [1700, 1708, 1745, 1756, 3166, 4002, 4010, 4036], '180–262': [1701], '1A,': [1704], 'full-length': [1705], 'SNAP-25A,': [1706], '1–96': [1709], 'II,': [1712, 4257], 'prepared': [1714, 1770, 1967, 2091, 2393, 3985], 'C2A-TMRWT': [1743, 1806], '1–265': [1746], 'naturally': [1753, 1780], 'Trp': [1755, 1782, 1956, 1963, 2674, 4035, 4060, 4099], 'positions': [1758], '58': [1759], '259.': [1761], 'version': [1764, 2462], 'fragment,': [1767], 'C2A-TMRTrp,': [1768, 1831], 'W58F': [1772], 'W259F': [1774], 'substitution': [1775, 3246], 'remove': [1778], 'residues.': [1783], 'third': [1785, 3634], 'mutation,': [1786], 'F234W,': [1787], 'introduced': [1789], 'such': [1790], 'construct': [1793, 1887], 'Trp,': [1797], 'lipid-binding': [1801, 4047], 'loop': [1802, 1954, 1961, 2739, 2744, 4048], '(11).': [1804], 'Recombinant': [1805], 'C2A-TMRTrp': [1808, 1921, 4115, 4139, 4158, 4217, 4243, 4261, 4287, 4337], 'generated': [1810, 4219], 'subcloning': [1816], 'pGEX-2T': [1818], 'Biotech).': [1821], 'Proteins': [1822], 'above.': [1829], 'Soluble': [1830], 'liposome': [1836, 2114, 3255, 3871], 'reconstitution': [1837, 4168, 4237], 'experiments,': [1838, 1948, 2126, 2138, 2655, 4169], 'pTrcHis': [1843], 'vector': [1845], '(InVitrogen)': [1846], 'resulting': [1873], 'His6-/T7-tag': [1881], 'N': [1884], 'terminus.': [1885], 'Ni-NTA-agarose': [1891], '(Qiagen)': [1892], 'After': [1919], 'purification,': [1920], 'dialyzed': [1923], 'against': [1924, 2041, 2240], '4': [1925, 2042, 2057, 2263, 2544], 'liters': [1926, 2043], 'HEPES': [1928, 2026, 2045, 2550, 4255], 'buffer': [1929, 2027, 2046, 2552, 2569, 4256, 4273, 4320], '(50': [1931, 2048], 'mm': [1932, 2314, 2319, 2330, 2334, 2556, 2560, 2572, 2576, 2633, 2753, 2759, 2764, 4314], 'HEPES-NaOH,': [1933], 'pH': [1934, 2050, 2316, 4272], '7.4,': [1935, 2051, 2317], '0.1': [1936, 2052], 'mNaCl,': [1937], '1%': [1939], 'sodium': [1940], 'cholate)': [1941], 'overnight.': [1942], 'For': [1943, 2059, 2136, 2649, 3001], 'acrylamide': [1944], 'brominated-lipid': [1946], 'quenching': [1947, 2671, 2715, 2793], 'C2A,': [1950, 3244, 4136], 'either': [1952, 2554, 2751], '(Trp-173)': [1958], '(Trp-234)': [1965], 'Brain-derived': [1996], '(PS),': [1998], '(PC),': [2000], '(dansyl-PE),': [2003], '(6,7-Br2-PC)': [2005, 2977], '(11,12-Br2-PC)': [2008], 'obtained': [2010], 'Avanti': [2012, 2094], 'Polar': [2013, 2095], 'Lipids': [2014, 2096], '(Alabaster,': [2015], 'AL).': [2016], 'prepare': [2018], 'proteoliposomes,': [2019], 'lipids': [2020, 3004, 3398], 'dried': [2022], 'dissolved': [2024], 'containing': [2029, 2627], '2': [2030, 2177, 2329, 2337, 2352, 2555, 2571, 2628, 2722, 2752, 2931, 2968], 'μm': [2031, 2173, 2537, 2629, 2734], 'Proteoliposomes': [2033, 2179], 'formed': [2035], 'dialyzing': [2037], 'lipid/detergent/protein': [2039], 'mixture': [2040], 'mmHEPES-NaOH,': [2049], 'm': [2053, 2723, 4268, 4278], 'NaCl)': [2054, 2320], 'overnight': [2055], '°C.': [2058, 2264], 'studies,': [2061], '(∼100': [2063], 'nm)': [2064], 'unilamellar': [2065], 'liposomes': [2066, 2640, 2658, 2762, 3888, 3900, 4117, 4142], '(26MacDonald': [2067], 'R.C.': [2068], 'MacDonald': [2069], 'R.I.': [2070], 'Menco': [2071], 'B.P.M.': [2072], 'Takeshita': [2073], 'Subbarao': [2075], 'N.K.': [2076], 'Hu': [2077], 'L.R.': [2078], 'Biochim.': [2079], 'Acta.': [2081], '1991;': [2082], '1061:': [2083], '297-303Crossref': [2084], '(1404)': [2087], 'extruder': [2097], 'according': [2098, 2796, 2867], "manufacturer's": [2101], 'instructions.': [2102], 'l-3-Phosphatidyl[N-methyl-3H]choline-1,2-dipalmitoyl': [2103], '([3H]PC)': [2104], 'purchased': [2106], 'Amersham': [2108], 'Biotech.3H-Labeled': [2110], '25%': [2111, 2662], 'PS/75%': [2112], 'PC': [2113], 'assays': [2116], '27).': [2123], 'all': [2125, 2514, 3040, 3790, 3835, 3908, 4033, 4258], 'error': [2127], 'bars': [2128], 'represent': [2129], 'standard': [2131], 'deviations': [2132], 'triplicate': [2134], 'determinations.': [2135], 'Ca2+titration': [2137], '[Ca2+]free': [2139], 'MI-600': [2144], 'electrode': [2146, 2151], 'MI-402': [2149], 'microreference': [2150], '(Microelectrode': [2152], 'Inc.,': [2153], 'Bedford,': [2154], 'NH)': [2155], 'World': [2157], 'Precision': [2158], 'Instruments': [2159], 'Inc.': [2160], '(Sarasota,': [2161], 'FL)': [2162], 'standards': [2164], '(pCa2+': [2165], 'range': [2166], '1–8).': [2168], 'concentrations': [2170], 'below': [2171], '100': [2172], 'buffered': [2175], 'mmEGTA.': [2178], '(75': [2180], 'μl': [2181, 2356, 2548], 'aliquots)': [2182], 'collected': [2184, 2708], 'centrifugation': [2186], '100,000': [2188], '×g': [2189], '30': [2191], 'min': [2192, 2209], 'Airfuge': [2195], '(Beckman).': [2196], 'Pelleted': [2197], 'proteoliposomes': [2198, 3942, 4227, 4244], 'resuspended': [2200], 'indicated': [2203, 3350, 3408], 'buffers': [2204], 'incubated': [2206, 2305, 2534], '20': [2208, 2313, 2517], 'room': [2211], 'temperature,': [2212], 'again': [2214], 'subjected': [2215, 2225, 2583], 'centrifugation.': [2217], '8%': [2218], 'supernatants': [2221], 'pellets': [2223], 'SDS-PAGE': [2227, 2376, 2585], 'immunoblot': [2229, 2587], 'analysis': [2230, 2588, 2870, 4348], 'anti-synaptotagmin': [2232], 'monoclonal': [2233, 2237, 2590], 'antibodies': [2234, 2238, 2401, 2592, 3662], '(41.1).': [2235], 'Mouse': [2236], 'directed': [2239], '(69.1)': [2242], '(41.1)': [2245], '(Göttingen,': [2252], 'Germany).': [2253], 'immunoprecipitation': [2255], 'bead-binding': [2257], 'Immunoprecipitation': [2265], 'recombinant': [2267, 2307], 'complexes': [2269, 2303, 2433, 3659], 'Briefly,': [2301], 'Tris-buffered': [2310], 'saline': [2311], '(TBS;': [2312], 'Tris,': [2315], '150': [2318, 2547], 'plus': [2321, 2570, 2761], '0.5%': [2322], 'Triton': [2323, 4295, 4319], 'X-100': [2324], 'presence': [2327, 2749, 2818, 3665, 3876], 'EGTA': [2331, 2557, 2573, 3667], 'h.': [2338, 2366], 'Synaptobrevin': [2339], 'immunoprecipitated': [2341], 'incubating': [2343], 'samples': [2345, 2390, 2430, 2581, 2617], '69.1': [2347], '(1.5': [2348], 'μl)': [2349], 'ascites': [2350], 'h': [2353, 2545], '12': [2355], 'G-Sepharose': [2359], 'Fast-Flow': [2360], 'immunoprecipitates': [2368], 'washed': [2370, 2564], 'times': [2372, 2566], 'analyzed': [2374, 2795, 3612], 'staining': [2378], 'Coomassie': [2380], 'blue.': [2381], 'As': [2382, 3230, 3670, 3881, 4248], 'control': [2384], 'nonspecific': [2386], 'precipitation': [2387], 'synaptotagmin,': [2389, 2405, 3843], 'lacking': [2394, 2431], 'SNAREs.': [2395, 2422], 'each': [2397], 'case,': [2398], 'immunoprecipitating': [2400, 2437], 'did': [2402, 2415, 3752, 3762, 3894, 3897], 'not': [2403, 2416, 3356, 3753, 3898], 'and,': [2406], 'under': [2407, 4230, 4245], 'conditions': [2409], 'assays,': [2413], 'precipitate': [2417], 'absence': [2420, 2816, 3878], 'Thus,': [2423, 4056], 'shown': [2426, 3231, 3516, 3671, 3882, 4065, 4249], 'Fig.': [2428, 3673, 4251], '1,': [2429], '(−)': [2434], 'lacked': [2436], 'antibodies.': [2438], 'Co-immunoprecipitation': [2439], 'quantified': [2443], 'Bio-Rad': [2446], 'GS-670': [2447], 'imaging': [2448], 'densitometer.': [2449], 'avoid': [2451], 'increases': [2452], 'co-immunoprecipitation': [2455, 2515, 3655], 'due': [2456], 'oligomerization,': [2459], 'nonoligomerizing': [2461], 'experiments.': [2516], 'μg': [2518], 'GST,': [2520], 'GST-C2A-TMR': [2521], '(amino': [2522, 2540], '1–265),': [2524], 'GST-C2A': [2525], '(),': [2526], 'GST-synaptobrevin': [2528], '(full-length)': [2529], 'His6-C2A-TMR': [2539], '1–265)': [2542], 'Ca2+.': [2561, 2577, 3669, 3736, 3880, 3904], 'Beads': [2562], '7%': [2578], 'Penta-His': [2591], '(Qiagen).': [2593], 'Immunoreactive': [2594], 'bands': [2595], 'visualized': [2597], 'enhanced': [2599], 'chemiluminescence.': [2600], 'Steady-state': [2601], 'measurements': [2603, 2716], 'made': [2605], '24': [2607, 2689], '°C': [2608, 2690], 'PTI': [2611], 'QM-1': [2612], 'fluorometer': [2613], 'Felix': [2615], 'software,': [2616], 'mixed': [2619, 2636], 'castle-style': [2622], 'stir': [2623], 'bar.': [2624], 'Proteoliposome': [2625], 'suspensions': [2626], '0.75': [2632], 'free': [2639, 2657, 3977], 'excited': [2642, 2700], '285': [2644, 2702], 'nm': [2645, 2784], '2-nm': [2647], 'resolution.': [2648], '(FRET)': [2654], 'contained': [2659], '5%': [2660], 'dansyl-PE,': [2661], 'PS,': [2663], '70%': [2665], 'PC.': [2666], 'FRET': [2667], 'monitored': [2669], 'donor': [2675], 'dansyl': [2679], 'acceptor.': [2680], 'Stopped-flow': [2681], 'mixing': [2683], 'Applied': [2693], 'Photophysics': [2694, 2854], 'SX.18MV': [2695], 'stopped-flow': [2696], 'spectrometer.': [2697], 'Samples': [2698], 'nm,': [2703], 'emitted': [2705], 'light': [2706], '326.1-nm': [2711], 'bandpass': [2712], 'filter.': [2713], 'Acrylamide': [2714], 'stock': [2724], 'solution': [2725, 2851], 'acrylamide.': [2727], 'quencher': [2729, 2945, 2957], 'added': [2731], '7.5': [2733], 'C2A-Trp': [2735], '(Trp-173': [2737], 'Trp-234': [2742], '3,': [2745, 4049], 'respectively)': [2746], 'EGTA,': [2754], 'mmCa2+': [2756], '(2': [2763], 'lipid;': [2765], '75%PC/25%PS)': [2766], 'indicated.': [2768], 'emission': [2770, 4095], 'spectra': [2771], 'corrected': [2773], 'blank,': [2775], 'dilution,': [2776], 'instrument': [2778], 'integrated': [2781], '300–400': [2783], 'yield': [2786], 'intensity.': [2789], 'degree': [2791, 4234], 'Stern-Volmer': [2799, 2826], 'equation': [2800, 2840], '(Equation': [2801], '1):': [2802], 'Fo/F=1+KSV[Q]Equation': [2803], 'where': [2805, 2932, 4001], 'F': [2806, 2809, 2967], 'o': [2807], 'intensities': [2813, 2973], 'acrylamide,': [2820], 'respectively,': [2821, 2982], 'K': [2822], 'SV': [2823], 'constant': [2827], 'collisional': [2829], 'quenching,': [2830], '[Q]': [2832], 'concentration': [2835], 'quencher.': [2838], 'predicts': [2841], 'linear': [2843], 'plot': [2844], 'ofF': [2845], 'o/F': [2846], 'versus[Q]': [2847], 'homogeneous': [2850], '(28Birks': [2852], 'J.B.': [2853], 'Aromatic': [2856], 'Molecules.': [2857], 'Wiley-Interscience,': [2858], 'New': [2859], 'York1970Google': [2860], 'measurement': [2864], 'performed': [2866], 'parallax': [2869], '(29Abrams': [2871], 'F.S.': [2872], 'London': [2873, 2886, 2903], '1992;': [2876], '31:': [2877], '5312-5322Crossref': [2878], '(105)': [2881], '30Chattopadhyay': [2884], '1987;': [2889, 3019], '26:': [2890, 3020], '39-45Crossref': [2891], '(604)': [2894], '31Ren': [2897], 'Lew': [2899], 'Wang': [2901], 'Z.': [2902], '36:': [2907], '10213-10220Crossref': [2908], '(191)': [2911], 'Specifically,': [2914], 'distance': [2916, 2937], 'residue': [2920, 3353], 'center': [2924, 2941], '(Z': [2925], 'CF)': [2926], 'given': [2928], 'Equation2:ZCF=LC1+[−ln(F1/F2)/πC−L2]/2LEquation': [2930], 'L': [2933, 2984], 'C1': [2934], 'represents': [2935], 'shallow': [2944, 2976], '(11': [2946], 'Å': [2947, 2996, 3013], '6,7-Br2-PC),': [2949], 'C': [2950], 'mole': [2953], 'fraction': [2954], 'divided': [2958], 'area': [2962], '(70': [2963], 'Å2),F': [2964], 'relative': [2971], 'quenchers': [2980, 2994], '(11,12-Br2-PC),': [2981], 'difference': [2987], '(0.9': [2995], 'per': [2997], 'CH2': [2998], 'CBr2group).': [3000], 'brominated': [3003], 'thickness': [3006], 'hydrophobic': [3009], '∼29': [3012], '(32McIntosh': [3014], 'T.J.': [3015], 'Holloway': [3016], 'P.W.': [3017], '1783-1788Crossref': [3021], '(166)': [3024], 'Our': [3027], 'goals': [3029], 'confirm': [3032, 3831], 'PS/PC': [3043, 3254, 3337], 'activity': [3045, 3257, 3328, 3339, 3760, 3841, 3873, 3914], 'intact': [3048, 3260], '27Davletov': [3070], '268:': [3078, 3284], '26386-26390Abstract': [3079, 3285], '33Chapman': [3086], 'P.I.': [3089, 3372, 3703], '270:': [3098, 3381, 3712], '23667-23671Abstract': [3099, 3382, 3713], '34Schiavo': [3110], 'Gu': [3112], 'Q.M.': [3113], 'Prestwich': [3114], 'G.D.': [3115], '93:': [3128], '13327-13332Crossref': [3129], '(261)': [3132], 'compare': [3137], 'roles': [3139], 'ligands': [3143, 3177, 3203, 3242, 3316, 3344, 3623, 3642, 3794], 'mediating': [3149], 'Structural': [3158], 'indicate': [3160, 3334, 4156, 4327], 'five': [3162, 3313, 3639], 'acidic': [3163, 3240], 'acid': [3165], '(Asp-172,': [3167], '-178,': [3168], '-230,': [3169], '-232,': [3170], '-238)': [3172], 'critical': [3175, 3530], 'within': [3178, 3243, 3345, 3624, 3643, 3803, 4044], 'precisely': [3205], 'Fig.1': [3233, 3518, 3775], 'A,': [3234, 3885, 4253], 'neutralization': [3235, 3308, 3424], 'any': [3237, 3755], 'Asn': [3248], 'Ser': [3250], 'residues,': [3251], 'abolished': [3252, 3422], 'synaptotagmin.': [3264], 'data': [3266, 3333, 3771, 4155, 4326], 'consistent': [3268], 'isolated': [3273, 3414, 3856, 4124], '(27Davletov': [3276], '35Zhang': [3292], '57:': [3300], '12395-12403Crossref': [3301], '(169)': [3303], 'contrast,': [3307, 3748], 'four': [3310, 3636], 'putative': [3314], '(D309N/D363N/D365N/D371N)': [3321], 'had': [3322], 'little': [3323], 'effect': [3324], 'PS/PC-binding': [3327], '(Fig.': [3329, 4129], 'A).': [3331], 'regulated': [3341], 'previous': [3348, 3695, 4068], 'Asp': [3352], '178': [3354], 'essential': [3357], '(33Chapman': [3369, 3700], 'uncoupled.': [3404], 'However,': [3405, 3737, 3807], 'other': [3406], 'reports': [3407, 3696], 'Asp-178': [3426], '(36Li': [3427], 'Ullrich': [3429], 'J.Z.': [3432], 'Anderson': [3433], 'R.G.W.': [3434], 'Brose': [3435], 'N.': [3436], '375:': [3441], '594-599Crossref': [3442], '(543)': [3445], 're-examined': [3450], 'assaying': [3454], 'ability': [3456], 'wild': [3458, 3678, 4024], 'type': [3459, 3679, 4025], 'assemble': [3470], 'onto': [3471, 4178, 4202], 'syntaxin,': [3481], 'SNAP-25,': [3482], 'synaptobrevin.': [3484], 'fully': [3486, 4160], 'SDS-resistant': [3488], '(20Hayashi': [3491], 'B': [3519, 3675, 3776], '(right': [3520, 3777], 'upper': [3521], 'panel).': [3522], 'Because': [3523], 'both': [3524], 'mutants.': [3615], 'Two': [3616, 4016], 'mutants': [3617], 'possessed': [3618], '(D178N': [3628], 'D230,232N)': [3630], 'neutralized': [3648], '(D309N/D363N/D365N/D371N).': [3649], 'Each': [3650], 'assayed': [3653, 3868, 4239], 'anti-synaptobrevin': [3661], '(left': [3676], 'panel),': [3677], 'D309N/D363N/D365N/D371N': [3681], 'bound': [3684, 3728, 3887, 4116], 'manner.': [3692], 'Consistent': [3693], 'synaptotagmin·syntaxin': [3698], 'D178N': [3725, 3809], '50%': [3738, 4298], 'Ca2+effect': [3741], 'inhibited': [3743], 'mutation.': [3746], 'D230,232N': [3750], 'exhibit': [3754], 'Ca2+-promoted': [3756], 'but': [3761], 'retain': [3763], 'Ca2+-independent': [3765], 'component': [3766], 'activity.': [3769], 'plotted': [3773], 'lower': [3778], 'panel)': [3779], 'demonstrate': [3781], 'that,': [3782], 'analogous': [3783], 'anionic': [3788, 3838, 3911], 'phospholipids,': [3789], 'lie': [3802], 'mutation': [3810], 'interactions,': [3820], 'distinct': [3826, 4204], 'structural': [3827], 'requirements.': [3828], 'formally': [3830], 'phospholipid': [3839, 3912], 'equimolar': [3844], 'amounts': [3845], 'C2AB),': [3851], 'well': [3853], 'radiolabeled': [3870], 'Fig.2': [3884], 'same': [3891, 3961, 4120, 4180], 'extent': [3892, 4121], 'Having': [3905], 'established': [3906], 'domain,': [3922], 'next': [3924], 'sought': [3925], 'generate': [3927], 'optical': [3929], 'follow': [3932], 'its': [3944], 'segment.': [3947], 'goal': [3949], 'embedded': [3967, 4186, 4290], 'encompasses': [3997], '1–265,': [4000], '58–79': [4003], 'correspond': [4004], '140–265': [4011], 'prepared,': [4022], '(C2A-TMRWT)': [4026], '(C2A-TMRTrp)': [4030], 'native': [4034], 'replaced': [4038], 'phenylalanines,': [4040], 'phenylalanine': [4042], '234,': [4043], 'Ca2+-': [4045], 'substituted': [4051], 'residue.': [4055], 'C2A-TMRTrpcontains': [4057], 'reporter,': [4061, 4100], 'study': [4069], 'Ca2+(11,': [4078], '12).': [4079], 'Membrane': [4080], 'results': [4082], 'increase': [4085], 'intensity': [4088], 'blue': [4091], 'shift': [4092], 'spectrum': [4096], 'thus': [4101], 'providing': [4102], 'convenient': [4104], 'means': [4105], 'C2A·lipid': [4109], 'real-time.': [4112], '2A).': [4130], 'sensitivities': [4134], 'C2A-TMR,': [4137], 'identical': [4144], '(Fig.2': [4145], 'B;': [4146], '[Ca2+]1/2': [4147], '∼': [4148], '20–21': [4149], 'μm;': [4150], 'Hill': [4151], 'coefficient': [4152], '1.9–2.2).': [4153], 'active': [4161], 'suitable': [4164], 'use': [4166], 'below.': [4172], 'docks': [4177, 4201], '(i.e.': [4187, 4207], 'membrane;': [4191, 4211], 'here': [4193, 4213], 'atrans': [4215], 'His6-tagged': [4222], '"Experimental': [4231], 'Procedures."': [4232], 'successful': [4236], 'sedimentation': [4241, 4335], 'various': [4246], 'conditions.': [4247], 'co-sedimented': [4262], 'Addition': [4265, 4302], 'salt,': [4269], '(pH': [4274], '11)': [4275], '5': [4277], 'urea': [4279], 'failed': [4280], 'solubilize': [4282], 'protein,': [4284], 'efficiently': [4289], 'Surprisingly,': [4293], '2%': [4294, 4318], 'solubilized': [4296], 'only': [4297], 'C2A-TMRTrp.': [4301], 'chaotropic': [4305], 'agent': [4306, 4312], '(5': [4307], 'murea)': [4308], 'reducing': [4311], '(10': [4313], 'DTT)': [4315], 'resulted': [4321], 'complete': [4323], 'solubilization.': [4324], 'secondary': [4330], 'contributes': [4332], 'co-sedimentation': [4340], 'assay.': [4341], 'Therefore,': [4342], 'before': [4343], 'carrying': [4344], 'cis/trans': [4347], 'further': [4350], 'below,': [4351], 'examined': [4353], 'insolubili': [4355]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1982257516', 'counts_by_year': [{'year': 2024, 'cited_by_count': 1}, {'year': 2023, 'cited_by_count': 3}, {'year': 2022, 'cited_by_count': 1}, {'year': 2021, 'cited_by_count': 3}, {'year': 2020, 'cited_by_count': 4}, {'year': 2018, 'cited_by_count': 1}, {'year': 2017, 'cited_by_count': 1}, {'year': 2016, 'cited_by_count': 1}, {'year': 2015, 'cited_by_count': 3}, {'year': 2014, 'cited_by_count': 2}, {'year': 2013, 'cited_by_count': 4}], 'updated_date': '2024-12-08T17:57:30.271165', 'created_date': '2016-06-24'}
Publication Information

Basic Information

Access and Citation

AI Researcher Chatbot

Primary Location

Authors

Topics

Keywords

Related Works
