Title: HPLC Determination of Utibapril and its Diacid FPL 63674XX in Rodent Laboratory Diet Using Selective Extraction and Gradient Elution Chromatography
Abstract:Abstract Utibapril is a novel thiadiazoline that is currently under investigation as an antihypertensive agent. The major degradation product, a diacid FPL 63674XX, is biologically active. An HPLC met...Abstract Utibapril is a novel thiadiazoline that is currently under investigation as an antihypertensive agent. The major degradation product, a diacid FPL 63674XX, is biologically active. An HPLC method has been developed to simultaneously determine both utibapril and its diacid in rodent laboratory diet used to dose animals in long term toxicology studies. The method is based on liquid-solid extraction of the compounds followed by direct injection of the extract. Gradient elution chromatography is utilized to better separate the diacid from interferences. Recovery of utibapril and the diacid from the lab diet was found to be 97.6 ± 0.44% and 99.7 ± 2.8%, respectively (n=3) at 0.43% w/w levels. The separation is achieved on a octadecylsilane column using a flow rate of 0.75 ml/min and a column temperature of 70°C. The UV detector was set at 260 nm. A linear and step gradient program was established using mobile phases consisting of 0.05 MRead More
Publication Year: 1991
Publication Date: 1991-09-01
Language: en
Type: article
Indexed In: ['crossref']
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