Title: Measurements of Desmosine and Isodesmosine by Mass Spectrometry in COPD
Abstract: <h3>Objectives</h3> Application of mass spectrometry (MS) for direct measurements of desmosine (D) and isodesmosine (I) in urine, plasma, and sputum as markers of elastin degradation in patients with α<sub>1</sub>-antitrypsin deficiency (AATD) and non-AATD-related COPD. <h3>Background</h3> In COPD patients, the lungs undergo elastin injury, which can be monitored by measurements of D and I in body fluids as specific markers of elastin degradation using the specificity and sensitivity of MS. <h3>Methods</h3> Acid hydrolysis of blood plasma, 24-h urine and sputum measurements, followed by chromatographic separation for mass spectrometric analysis. <h3>Results</h3> Each patient group had levels of plasma D and I that were statistically significantly higher than those of control subjects. AATD patients had higher levels than COPD patients with normal α<sub>1</sub>-antitrypsin (AAT) levels. Twenty-four-hour urine measurements demonstrated no significant difference in total levels of D and I among control subjects and patients but showed a free (unbound) concentration of D and I in urine, which was statistically significantly higher in patients with COPD with and without AAT. The D and I levels in the sputum of patients with AATD exceeded the levels in COPD patients with normal AAT levels. <h3>Conclusions</h3> MS allows a sensitive and specific analysis of D and I in body fluids. The quantification of D and I in sputum, along with increases of D and I in plasma and an elevated free component of D and I in urine provide indexes that characterize patients with COPD and can be followed in relation to the course of the disease and/or therapy.
Publication Year: 2007
Publication Date: 2007-05-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 115
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