Title: Measurement of lysozyme by an enzyme-linked immunosorbent assay
Abstract: In this study an enzyme-linked immunosorbent assay has been developed for the determination of lysozyme in saliva, serum and urine. The assay relies on the detection of specific protein rather than lytic activity, a property which has been shown to be most suitable for the quantitation of lysozyme in mucin containing substances. Our results indicate that no pretreatment is necessary for the immunochemical method. The assay is sensitive to concentrations as low as 1 μg lysozyme/I. The intra-assay and inter-assay coefficients of variation were 5.9% and 15.8% respectively. The lysozyme level in whole saliva was 55.53 ± 30.35 mg/l, in serum the level was 0.64 ± 0.15 mg/l and in urine it was 0.17 ± 0.22 mg/l. Comparisons between immunochemical determination and lytic assays showed a good correlation (serum, r = 0.79, P < 0.01; saliva, r = 0.85, P < 0.005; treated saliva, r = 0.96, P < 0.001).
Publication Year: 1992
Publication Date: 1992-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 29
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