Title: Isoflavonoid Changes in Soybean Cell Suspensions when Callenged with Intact Bacteria or Fungal Elicitors
Abstract: Glycine max (L.) Merr. cv. Mandarin cell suspension cultures did not give a typical hypersensitive response (HR) when challenged with a fungal elicitor or an incompatible strain of the soybean leaf pathogen Pseudomonas syringae cv. glycinea (Psg). Those cultures that darkened showed a small, gradual loss of viability and accumulated the phytoalexin glyceollin. Such cultures had a much higher level of isoflavonoids than those that did not darken or produced glyceollin under biotic stress. Concurrent with the formation of glyceollin, there was a sharp decline in the level of daidzein and genistein. Genistein was identified as a major isoflavonoid of soybean cell suspensions, but occurred only in trace amounts in the intact plant. No correlation was found between the induced levels of glyceollin and constitutive levels of isoflavonoids. While daidzein may be a direct precursor of glyceollin, a high level of daidzein and perhaps genistein appeared to be indicative of a required physiological state of the culture for the stress response. Cell culture levels of isoflavonoids appeared to be elevated by light.
Publication Year: 1990
Publication Date: 1990-02-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 20
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