Title: Extracellular soluble polysaccharide (ESP) from Aspergillus kawachii improves the stability of extracellular β-gluocosidases (EX-1 and EX-2) and is involved in their localization
Abstract: Aspergillus kawachii produces two extracellular β-glucosidases (EX-1 and EX-2) and one cell-wall-bound β-glucosidase (CB-1), all of which are derived from the same bglA gene. Extracellular β-glucosidases (EX-1 and EX-2) are stable in the crude solution form, but become unstable in the purified form under moderate conditions (pH 5.0 and 37°C). Purified extracellular β-glucosidases can bind to a mycelial cell wall fraction, even though these enzymes are released into the medium under solid culture conditions. A. kawachii produces an extracellular soluble the β-glucosidases over the pH range of 3.0–7.0 and at temperatures below 50°C. ESP directly interacted with the purified extracellular β-glucosidases but did not affect the Km values of these enzymes. Moreover, ESP inhibited the adsorption of purified extracellular β-glucosidases to the cell wall fraction and extracted them from it. These results that ESP plays important roles in the stability and localization of extracellular β-glucosidases. ESP from A. kawachii directly binds to the enzymes and releases them to the medium from the cell wall layer and then stabilizes them.
Publication Year: 2001
Publication Date: 2001-01-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 18
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