Title: Influence of the amino acid sequence and nature of the cyclodextrin on the separation of small peptide enantiomers by capillary electrophoresis using α-, β-, and γ-cyclodextrin and the corresponding hydroxypropyl derivatives
Abstract: Journal of Separation ScienceVolume 24, Issue 9 p. 777-783 Research Article Influence of the amino acid sequence and nature of the cyclodextrin on the separation of small peptide enantiomers by capillary electrophoresis using α-, β-, and γ-cyclodextrin and the corresponding hydroxypropyl derivatives Nino Sidamonidze, Nino Sidamonidze University of Jena, School of Pharmacy, Department of Pharmaceutical Chemistry, Philosophenweg 14, D-07743 Jena, Germany; Fax: + 49 3641 949802Search for more papers by this authorFalko Süß, Falko Süß University of Jena, School of Pharmacy, Department of Pharmaceutical Chemistry, Philosophenweg 14, D-07743 Jena, Germany; Fax: + 49 3641 949802Search for more papers by this authorWolfgang Poppitz, Wolfgang Poppitz University of Jena, Department of Inorganic and Analytical Chemistry, August-Bebel-Strasse 2, D-07743 Jena, GermanySearch for more papers by this authorGerhard K. E. Scriba, Gerhard K. E. Scriba [email protected] Search for more papers by this author Nino Sidamonidze, Nino Sidamonidze University of Jena, School of Pharmacy, Department of Pharmaceutical Chemistry, Philosophenweg 14, D-07743 Jena, Germany; Fax: + 49 3641 949802Search for more papers by this authorFalko Süß, Falko Süß University of Jena, School of Pharmacy, Department of Pharmaceutical Chemistry, Philosophenweg 14, D-07743 Jena, Germany; Fax: + 49 3641 949802Search for more papers by this authorWolfgang Poppitz, Wolfgang Poppitz University of Jena, Department of Inorganic and Analytical Chemistry, August-Bebel-Strasse 2, D-07743 Jena, GermanySearch for more papers by this authorGerhard K. E. Scriba, Gerhard K. E. Scriba [email protected] Search for more papers by this author First published: 25 October 2001 https://doi.org/10.1002/1615-9314(20010901)24:9<777::AID-JSSC777>3.0.CO;2-VCitations: 37AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The separation of the LL and DD enantiomers of dipeptides and tripeptides using α-, β-, and γ-cyclodextrins as well as the corresponding hydroxypropyl derivatives was studied with respect to the amino acid sequence of the peptides and the nature of the cyclodextrins. Standardized conditions regarding buffer pH and molarity, cyclodextrin concentration, and separation voltage were applied. α-Cyclodextrin, hydroxypropyl-α-cyclodextrin, β-cyclodextrin, and hydroxypropyl-β-cyclodextrin were the more universal cyclodextrins for enantioseparations of the investigated set of peptides compared to γ-cyclodextrin and hydroxypropyl-γ-cyclodextrin. The enantiomer migration order depended both on the cyclodextrin and on the amino acid sequence of the peptide. Reversal of the enantiomer migration order upon increasing the buffer pH from 2.5 to 3.5 was observed in some cases using β-cyclodextrin. References 1 V. Kasicka Electrophoresis, 1999, 20, 3084–3105. 2 H. Wan, L. G. Blomberg J. Chromatogr. A 2000, 875, 43–88. 3 G. M. Janini, G. M. Muschik, H. Issaq Electrophoresis, 1996, 17, 1575–1583. 4 D. J. Skanchy, R. Wilson, T. Poh, G. H. Xie, C. W. Demarest, J. F. Stobaugh Electrophoresis, 1997, 18, 985–995. 5 C. E. Sänger Van De Griend, K. Gröningen, T. Arvidson J. 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Publication Year: 2001
Publication Date: 2001-09-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 36
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