Get quick answers to your questions about the article from our AI researcher chatbot
{'id': 'https://openalex.org/W1977238514', 'doi': 'https://doi.org/10.1111/j.1447-0756.1987.tb00019.x', 'title': 'Primary Culture of Cells Derived from Human Myometrium: Both Myometrium and Cultured Cells Stained with Desmin by the Enzyme‐Labeled Antibody Method', 'display_name': 'Primary Culture of Cells Derived from Human Myometrium: Both Myometrium and Cultured Cells Stained with Desmin by the Enzyme‐Labeled Antibody Method', 'publication_year': 1987, 'publication_date': '1987-03-01', 'ids': {'openalex': 'https://openalex.org/W1977238514', 'doi': 'https://doi.org/10.1111/j.1447-0756.1987.tb00019.x', 'mag': '1977238514', 'pmid': 'https://pubmed.ncbi.nlm.nih.gov/3297011'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.1111/j.1447-0756.1987.tb00019.x', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2753990315', 'display_name': 'Asia-Oceania Journal of Obstetrics and Gynaecology', 'issn_l': '0389-2328', 'issn': ['0389-2328'], 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/P4310313366', 'host_organization_name': 'University of Tokyo Press', 'host_organization_lineage': ['https://openalex.org/P4310313366', 'https://openalex.org/P4310313595'], 'host_organization_lineage_names': ['University of Tokyo Press', 'University of Tokyo'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref', 'pubmed'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5019942640', 'display_name': 'Haruo Otsuka', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I188700360', 'display_name': 'University of Occupational and Environmental Health Japan', 'ror': 'https://ror.org/020p3h829', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I188700360']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Haruo Otsuka', 'raw_affiliation_strings': ['Department of Obstetrics and Gynecology, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan'], 'affiliations': [{'raw_affiliation_string': 'Department of Obstetrics and Gynecology, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan', 'institution_ids': ['https://openalex.org/I188700360']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5085118456', 'display_name': 'Kohji Yoshida', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I188700360', 'display_name': 'University of Occupational and Environmental Health Japan', 'ror': 'https://ror.org/020p3h829', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I188700360']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Kohji Yoshida', 'raw_affiliation_strings': ['Department of Obstetrics and Gynecology, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan'], 'affiliations': [{'raw_affiliation_string': 'Department of Obstetrics and Gynecology, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan', 'institution_ids': ['https://openalex.org/I188700360']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5017114596', 'display_name': 'Shuichi Kusano', 'orcid': 'https://orcid.org/0000-0001-6293-486X'}, 'institutions': [{'id': 'https://openalex.org/I188700360', 'display_name': 'University of Occupational and Environmental Health Japan', 'ror': 'https://ror.org/020p3h829', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I188700360']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Shuichi Kusano', 'raw_affiliation_strings': ['Department of Obstetrics and Gynecology, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan'], 'affiliations': [{'raw_affiliation_string': 'Department of Obstetrics and Gynecology, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan', 'institution_ids': ['https://openalex.org/I188700360']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5012046256', 'display_name': 'Y Okamura', 'orcid': 'https://orcid.org/0000-0002-0783-8450'}, 'institutions': [{'id': 'https://openalex.org/I188700360', 'display_name': 'University of Occupational and Environmental Health Japan', 'ror': 'https://ror.org/020p3h829', 'country_code': 'JP', 'type': 'education', 'lineage': ['https://openalex.org/I188700360']}], 'countries': ['JP'], 'is_corresponding': False, 'raw_author_name': 'Yasushi Okamura', 'raw_affiliation_strings': ['Department of Obstetrics and Gynecology, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan'], 'affiliations': [{'raw_affiliation_string': 'Department of Obstetrics and Gynecology, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan', 'institution_ids': ['https://openalex.org/I188700360']}]}], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 0.0, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 0, 'citation_normalized_percentile': {'value': 0.0, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 0, 'max': 53}, 'biblio': {'volume': '13', 'issue': '1', 'first_page': '121', 'last_page': '126'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T11650', 'display_name': 'Biology and Pathology of Keratins and Related Disorders', 'score': 0.9955, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T11650', 'display_name': 'Biology and Pathology of Keratins and Related Disorders', 'score': 0.9955, 'subfield': {'id': 'https://openalex.org/subfields/1307', 'display_name': 'Cell Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T11966', 'display_name': 'Biomedical Applications of Silk Biomaterials', 'score': 0.9821, 'subfield': {'id': 'https://openalex.org/subfields/2502', 'display_name': 'Biomaterials'}, 'field': {'id': 'https://openalex.org/fields/25', 'display_name': 'Materials Science'}, 'domain': {'id': 'https://openalex.org/domains/3', 'display_name': 'Physical Sciences'}}, {'id': 'https://openalex.org/T10831', 'display_name': 'Integrin Signaling in Inflammation and Cancer', 'score': 0.96, 'subfield': {'id': 'https://openalex.org/subfields/2723', 'display_name': 'Immunology and Allergy'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/myometrium', 'display_name': 'Myometrium', 'score': 0.9875048}, {'id': 'https://openalex.org/keywords/desmin', 'display_name': 'Desmin', 'score': 0.90736294}, {'id': 'https://openalex.org/keywords/cell-adhesion', 'display_name': 'Cell Adhesion', 'score': 0.48836}], 'concepts': [{'id': 'https://openalex.org/C2778164554', 'wikidata': 'https://www.wikidata.org/wiki/Q2562472', 'display_name': 'Myometrium', 'level': 3, 'score': 0.9875048}, {'id': 'https://openalex.org/C2779072444', 'wikidata': 'https://www.wikidata.org/wiki/Q425146', 'display_name': 'Desmin', 'level': 4, 'score': 0.90736294}, {'id': 'https://openalex.org/C159654299', 'wikidata': 'https://www.wikidata.org/wiki/Q79460', 'display_name': 'Antibody', 'level': 2, 'score': 0.6144864}, {'id': 'https://openalex.org/C81885089', 'wikidata': 'https://www.wikidata.org/wiki/Q189082', 'display_name': 'Cell culture', 'level': 2, 'score': 0.48667675}, {'id': 'https://openalex.org/C2780340462', 'wikidata': 'https://www.wikidata.org/wiki/Q127450', 'display_name': 'Trypsin', 'level': 3, 'score': 0.450557}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.43372077}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.41748646}, {'id': 'https://openalex.org/C16685009', 'wikidata': 'https://www.wikidata.org/wiki/Q335057', 'display_name': 'Andrology', 'level': 1, 'score': 0.40132433}, {'id': 'https://openalex.org/C153911025', 'wikidata': 'https://www.wikidata.org/wiki/Q7202', 'display_name': 'Molecular biology', 'level': 1, 'score': 0.4009177}, {'id': 'https://openalex.org/C2779066055', 'wikidata': 'https://www.wikidata.org/wiki/Q9612', 'display_name': 'Uterus', 'level': 2, 'score': 0.39736095}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.36060447}, {'id': 'https://openalex.org/C134018914', 'wikidata': 'https://www.wikidata.org/wiki/Q162606', 'display_name': 'Endocrinology', 'level': 1, 'score': 0.24941263}, {'id': 'https://openalex.org/C204232928', 'wikidata': 'https://www.wikidata.org/wiki/Q899285', 'display_name': 'Immunohistochemistry', 'level': 2, 'score': 0.18818697}, {'id': 'https://openalex.org/C203014093', 'wikidata': 'https://www.wikidata.org/wiki/Q101929', 'display_name': 'Immunology', 'level': 1, 'score': 0.14568546}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.14401007}, {'id': 'https://openalex.org/C147447768', 'wikidata': 'https://www.wikidata.org/wiki/Q411624', 'display_name': 'Vimentin', 'level': 3, 'score': 0.12654838}, {'id': 'https://openalex.org/C71924100', 'wikidata': 'https://www.wikidata.org/wiki/Q11190', 'display_name': 'Medicine', 'level': 0, 'score': 0.09617615}, {'id': 'https://openalex.org/C54355233', 'wikidata': 'https://www.wikidata.org/wiki/Q7162', 'display_name': 'Genetics', 'level': 1, 'score': 0.0}], 'mesh': [{'descriptor_ui': 'D003893', 'descriptor_name': 'Desmin', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': True}, {'descriptor_ui': 'D009215', 'descriptor_name': 'Myometrium', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': True}, {'descriptor_ui': 'D000328', 'descriptor_name': 'Adult', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D002478', 'descriptor_name': 'Cells, Cultured', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D003893', 'descriptor_name': 'Desmin', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D005260', 'descriptor_name': 'Female', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D006801', 'descriptor_name': 'Humans', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007124', 'descriptor_name': 'Immunoenzyme Techniques', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007889', 'descriptor_name': 'Leiomyoma', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D007889', 'descriptor_name': 'Leiomyoma', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': False}, {'descriptor_ui': 'D007889', 'descriptor_name': 'Leiomyoma', 'qualifier_ui': 'Q000473', 'qualifier_name': 'pathology', 'is_major_topic': False}, {'descriptor_ui': 'D008875', 'descriptor_name': 'Middle Aged', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D009215', 'descriptor_name': 'Myometrium', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014594', 'descriptor_name': 'Uterine Neoplasms', 'qualifier_ui': '', 'qualifier_name': None, 'is_major_topic': False}, {'descriptor_ui': 'D014594', 'descriptor_name': 'Uterine Neoplasms', 'qualifier_ui': 'Q000032', 'qualifier_name': 'analysis', 'is_major_topic': False}, {'descriptor_ui': 'D014594', 'descriptor_name': 'Uterine Neoplasms', 'qualifier_ui': 'Q000473', 'qualifier_name': 'pathology', 'is_major_topic': False}], 'locations_count': 2, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.1111/j.1447-0756.1987.tb00019.x', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S2753990315', 'display_name': 'Asia-Oceania Journal of Obstetrics and Gynaecology', 'issn_l': '0389-2328', 'issn': ['0389-2328'], 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/P4310313366', 'host_organization_name': 'University of Tokyo Press', 'host_organization_lineage': ['https://openalex.org/P4310313366', 'https://openalex.org/P4310313595'], 'host_organization_lineage_names': ['University of Tokyo Press', 'University of Tokyo'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, {'is_oa': False, 'landing_page_url': 'https://pubmed.ncbi.nlm.nih.gov/3297011', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S4306525036', 'display_name': 'PubMed', 'issn_l': None, 'issn': None, 'is_oa': False, 'is_in_doaj': False, 'is_core': False, 'host_organization': 'https://openalex.org/I1299303238', 'host_organization_name': 'National Institutes of Health', 'host_organization_lineage': ['https://openalex.org/I1299303238'], 'host_organization_lineage_names': ['National Institutes of Health'], 'type': 'repository'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 9, 'referenced_works': ['https://openalex.org/W106282761', 'https://openalex.org/W1275350575', 'https://openalex.org/W1469270584', 'https://openalex.org/W1979937757', 'https://openalex.org/W2022531336', 'https://openalex.org/W2037812119', 'https://openalex.org/W2055390759', 'https://openalex.org/W2061721919', 'https://openalex.org/W2148133676'], 'related_works': ['https://openalex.org/W2143972452', 'https://openalex.org/W2125009908', 'https://openalex.org/W2066640119', 'https://openalex.org/W2063302025', 'https://openalex.org/W2021893861', 'https://openalex.org/W2020497474', 'https://openalex.org/W1992542653', 'https://openalex.org/W1973334688', 'https://openalex.org/W1968953447', 'https://openalex.org/W1906685812'], 'abstract_inverted_index': {'Abstract': [0], 'To': [1, 43], 'evaluate': [2], 'the': [3, 46, 54, 57, 64, 78], 'characteristics': [4], 'of': [5, 12], 'myometrial': [6], 'cells,': [7], 'we': [8, 75], 'studied': [9], 'primary': [10, 32], 'cultures': [11], 'cells': [13, 48, 58, 80], 'obtained': [14], 'from': [15, 51, 83], 'uterine': [16], 'muscle.': [17], 'The': [18, 31], 'myometrium': [19, 55], 'was': [20], 'cut': [21], 'into': [22], 'small': [23], 'pieces': [24], 'and': [25, 56], 'suspended': [26], 'in': [27], 'a': [28, 36, 73], 'trypsin': [29], 'solution.': [30], 'cell': [33], 'culture,': [34], 'as': [35], 'monolayer,': [37], 'could': [38], 'be': [39], 'passaged': [40], 'four': [41], 'times.': [42], 'confirm': [44], 'that': [45, 77], 'cultured': [47, 79], 'were': [49, 59, 69, 81], 'derived': [50, 82], 'myometrium,': [52], 'both': [53], 'stained': [60], 'with': [61], 'desmin': [62], 'by': [63], 'enzyme‐labeled': [65], 'antibody': [66], 'method.': [67], 'These': [68], 'all': [70], 'positive.': [71], 'As': [72], 'result,': [74], 'confirmed': [76], 'myometrium.': [84]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1977238514', 'counts_by_year': [], 'updated_date': '2024-08-27T22:41:43.810334', 'created_date': '2016-06-24'}