Title: The release of Fc′ and pFc′ fragments from human immunoglobulin G following enzymic digestion: A time study
Abstract: The techniques of high-resolution gel filtration, immunoelectrophoresis and starch-gel electrophoresis have been used to investigate the enzymic cleavage of human immunoglobulin G (IgG) as a function of time. In particular, the appearance and fate of Fc′ fragment (produced by papain) and pFc′ fragment (produced by pepsin) have been studied. These related fragments, which are located in the C-terminal part of the Fc fragment of the IgG molecule, are relatively resistant to enzymatic degradation whereas the N-terminal portion of the Fc fragment is rapidly degraded. Prolonged exposure to enzyme eventually breaks down both of these fragments but leaves the antibody-binding fragments (Fab or F(ab′)2 fragments) essentially intact.
Publication Year: 1969
Publication Date: 1969-03-01
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
Access and Citation
Cited By Count: 26
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