Publication Information

{'id': 'https://openalex.org/W1976087115', 'doi': 'https://doi.org/10.4028/www.scientific.net/amr.1033-1034.271', 'title': 'Site-Directed Mutagenesis of a Neutral Phytase from <i>Bacillus amyloliquefaciens</i>: Influencing Activity and Stability', 'display_name': 'Site-Directed Mutagenesis of a Neutral Phytase from <i>Bacillus amyloliquefaciens</i>: Influencing Activity and Stability', 'publication_year': 2014, 'publication_date': '2014-10-01', 'ids': {'openalex': 'https://openalex.org/W1976087115', 'doi': 'https://doi.org/10.4028/www.scientific.net/amr.1033-1034.271', 'mag': '1976087115'}, 'language': 'en', 'primary_location': {'is_oa': False, 'landing_page_url': 'https://doi.org/10.4028/www.scientific.net/amr.1033-1034.271', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S67425620', 'display_name': 'Advanced materials research', 'issn_l': '1022-6680', 'issn': ['1022-6680', '1662-8985'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310317839', 'host_organization_name': 'Trans Tech Publications', 'host_organization_lineage': ['https://openalex.org/P4310317839'], 'host_organization_lineage_names': ['Trans Tech Publications'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}, 'type': 'article', 'type_crossref': 'journal-article', 'indexed_in': ['crossref'], 'open_access': {'is_oa': False, 'oa_status': 'closed', 'oa_url': None, 'any_repository_has_fulltext': False}, 'authorships': [{'author_position': 'first', 'author': {'id': 'https://openalex.org/A5100944565', 'display_name': 'Wei Xu', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210157719', 'display_name': 'Yancheng Institute of Technology', 'ror': 'https://ror.org/04y8njc86', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I4210157719']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Wei Xu', 'raw_affiliation_strings': ['Yancheng Institute of Technology'], 'affiliations': [{'raw_affiliation_string': 'Yancheng Institute of Technology', 'institution_ids': ['https://openalex.org/I4210157719']}]}, {'author_position': 'middle', 'author': {'id': 'https://openalex.org/A5068823327', 'display_name': 'Zu Peng Wang', 'orcid': None}, 'institutions': [{'id': 'https://openalex.org/I4210157719', 'display_name': 'Yancheng Institute of Technology', 'ror': 'https://ror.org/04y8njc86', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I4210157719']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Zu Peng Wang', 'raw_affiliation_strings': ['Yancheng Institute of Technology'], 'affiliations': [{'raw_affiliation_string': 'Yancheng Institute of Technology', 'institution_ids': ['https://openalex.org/I4210157719']}]}, {'author_position': 'last', 'author': {'id': 'https://openalex.org/A5101770911', 'display_name': 'Rong Shao', 'orcid': 'https://orcid.org/0000-0001-8188-9785'}, 'institutions': [{'id': 'https://openalex.org/I4210157719', 'display_name': 'Yancheng Institute of Technology', 'ror': 'https://ror.org/04y8njc86', 'country_code': 'CN', 'type': 'education', 'lineage': ['https://openalex.org/I4210157719']}], 'countries': ['CN'], 'is_corresponding': False, 'raw_author_name': 'Rong Shao', 'raw_affiliation_strings': ['Yancheng Institute of Technology'], 'affiliations': [{'raw_affiliation_string': 'Yancheng Institute of Technology', 'institution_ids': ['https://openalex.org/I4210157719']}]}], 'institution_assertions': [], 'countries_distinct_count': 1, 'institutions_distinct_count': 1, 'corresponding_author_ids': [], 'corresponding_institution_ids': [], 'apc_list': None, 'apc_paid': None, 'fwci': 0.658, 'has_fulltext': True, 'fulltext_origin': 'ngrams', 'cited_by_count': 4, 'citation_normalized_percentile': {'value': 0.645308, 'is_in_top_1_percent': False, 'is_in_top_10_percent': False}, 'cited_by_percentile_year': {'min': 79, 'max': 81}, 'biblio': {'volume': '1033-1034', 'issue': None, 'first_page': '271', 'last_page': '278'}, 'is_retracted': False, 'is_paratext': False, 'primary_topic': {'id': 'https://openalex.org/T12991', 'display_name': 'Phytase and its Applications', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/1110', 'display_name': 'Plant Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, 'topics': [{'id': 'https://openalex.org/T12991', 'display_name': 'Phytase and its Applications', 'score': 1.0, 'subfield': {'id': 'https://openalex.org/subfields/1110', 'display_name': 'Plant Science'}, 'field': {'id': 'https://openalex.org/fields/11', 'display_name': 'Agricultural and Biological Sciences'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}, {'id': 'https://openalex.org/T10499', 'display_name': 'Folate and B Vitamins Research', 'score': 0.9901, 'subfield': {'id': 'https://openalex.org/subfields/2745', 'display_name': 'Rheumatology'}, 'field': {'id': 'https://openalex.org/fields/27', 'display_name': 'Medicine'}, 'domain': {'id': 'https://openalex.org/domains/4', 'display_name': 'Health Sciences'}}, {'id': 'https://openalex.org/T11561', 'display_name': 'Protein Hydrolysis and Bioactive Peptides', 'score': 0.9843, 'subfield': {'id': 'https://openalex.org/subfields/1312', 'display_name': 'Molecular Biology'}, 'field': {'id': 'https://openalex.org/fields/13', 'display_name': 'Biochemistry, Genetics and Molecular Biology'}, 'domain': {'id': 'https://openalex.org/domains/1', 'display_name': 'Life Sciences'}}], 'keywords': [{'id': 'https://openalex.org/keywords/bacillus-amyloliquefaciens', 'display_name': 'Bacillus amyloliquefaciens', 'score': 0.9308181}, {'id': 'https://openalex.org/keywords/phytase', 'display_name': 'Phytase', 'score': 0.9291792}, {'id': 'https://openalex.org/keywords/thermostability', 'display_name': 'Thermostability', 'score': 0.84042263}, {'id': 'https://openalex.org/keywords/site-directed-mutagenesis', 'display_name': 'Site-directed mutagenesis', 'score': 0.73349154}, {'id': 'https://openalex.org/keywords/directed-mutagenesis', 'display_name': 'Directed mutagenesis', 'score': 0.58850694}, {'id': 'https://openalex.org/keywords/wild-type', 'display_name': 'Wild type', 'score': 0.49519673}], 'concepts': [{'id': 'https://openalex.org/C2777811704', 'wikidata': 'https://www.wikidata.org/wiki/Q4354408', 'display_name': 'Bacillus amyloliquefaciens', 'level': 3, 'score': 0.9308181}, {'id': 'https://openalex.org/C109551439', 'wikidata': 'https://www.wikidata.org/wiki/Q410099', 'display_name': 'Phytase', 'level': 3, 'score': 0.9291792}, {'id': 'https://openalex.org/C2781216036', 'wikidata': 'https://www.wikidata.org/wiki/Q902494', 'display_name': 'Thermostability', 'level': 3, 'score': 0.84042263}, {'id': 'https://openalex.org/C143065580', 'wikidata': 'https://www.wikidata.org/wiki/Q3285695', 'display_name': 'Mutant', 'level': 3, 'score': 0.8263303}, {'id': 'https://openalex.org/C16318435', 'wikidata': 'https://www.wikidata.org/wiki/Q149299', 'display_name': 'Mutagenesis', 'level': 4, 'score': 0.7735413}, {'id': 'https://openalex.org/C103408237', 'wikidata': 'https://www.wikidata.org/wiki/Q2642668', 'display_name': 'Site-directed mutagenesis', 'level': 4, 'score': 0.73349154}, {'id': 'https://openalex.org/C69034178', 'wikidata': 'https://www.wikidata.org/wiki/Q4680754', 'display_name': 'Directed mutagenesis', 'level': 4, 'score': 0.58850694}, {'id': 'https://openalex.org/C207583985', 'wikidata': 'https://www.wikidata.org/wiki/Q128723', 'display_name': 'Wild type', 'level': 4, 'score': 0.49519673}, {'id': 'https://openalex.org/C55493867', 'wikidata': 'https://www.wikidata.org/wiki/Q7094', 'display_name': 'Biochemistry', 'level': 1, 'score': 0.46142533}, {'id': 'https://openalex.org/C40767141', 'wikidata': 'https://www.wikidata.org/wiki/Q285697', 'display_name': 'Recombinant DNA', 'level': 3, 'score': 0.44097114}, {'id': 'https://openalex.org/C181199279', 'wikidata': 'https://www.wikidata.org/wiki/Q8047', 'display_name': 'Enzyme', 'level': 2, 'score': 0.42872477}, {'id': 'https://openalex.org/C185592680', 'wikidata': 'https://www.wikidata.org/wiki/Q2329', 'display_name': 'Chemistry', 'level': 0, 'score': 0.42544696}, {'id': 'https://openalex.org/C86803240', 'wikidata': 'https://www.wikidata.org/wiki/Q420', 'display_name': 'Biology', 'level': 0, 'score': 0.40306357}, {'id': 'https://openalex.org/C104317684', 'wikidata': 'https://www.wikidata.org/wiki/Q7187', 'display_name': 'Gene', 'level': 2, 'score': 0.14041978}, {'id': 'https://openalex.org/C100544194', 'wikidata': 'https://www.wikidata.org/wiki/Q41760', 'display_name': 'Fermentation', 'level': 2, 'score': 0.067993134}], 'mesh': [], 'locations_count': 1, 'locations': [{'is_oa': False, 'landing_page_url': 'https://doi.org/10.4028/www.scientific.net/amr.1033-1034.271', 'pdf_url': None, 'source': {'id': 'https://openalex.org/S67425620', 'display_name': 'Advanced materials research', 'issn_l': '1022-6680', 'issn': ['1022-6680', '1662-8985'], 'is_oa': False, 'is_in_doaj': False, 'is_core': True, 'host_organization': 'https://openalex.org/P4310317839', 'host_organization_name': 'Trans Tech Publications', 'host_organization_lineage': ['https://openalex.org/P4310317839'], 'host_organization_lineage_names': ['Trans Tech Publications'], 'type': 'journal'}, 'license': None, 'license_id': None, 'version': None, 'is_accepted': False, 'is_published': False}], 'best_oa_location': None, 'sustainable_development_goals': [], 'grants': [], 'datasets': [], 'versions': [], 'referenced_works_count': 4, 'referenced_works': ['https://openalex.org/W1967234972', 'https://openalex.org/W2101448526', 'https://openalex.org/W2131272448', 'https://openalex.org/W2349861179'], 'related_works': ['https://openalex.org/W2980069888', 'https://openalex.org/W2891917486', 'https://openalex.org/W2421874819', 'https://openalex.org/W2415789484', 'https://openalex.org/W2412266719', 'https://openalex.org/W2394129813', 'https://openalex.org/W2358175167', 'https://openalex.org/W2087469207', 'https://openalex.org/W1976087115', 'https://openalex.org/W1148081604'], 'abstract_inverted_index': {'In': [0], 'order': [1], 'to': [2, 57], 'improve': [3], 'the': [4, 21, 27, 40, 66, 83, 92, 117, 133, 145, 148], 'activity': [5, 103, 158], 'and': [6, 43, 63, 70, 91, 159], 'stability': [7], 'of': [8, 30, 46, 74, 86, 104, 116, 124, 132, 147], 'phytase': [9], 'from': [10, 49], 'Bacillus': [11], 'amyloliquefaciens': [12], ',': [13], 'site-directed': [14], 'mutagenesis': [15, 53], 'has': [16], 'been': [17], 'performed': [18], 'base': [19], 'on': [20, 39], 'previous': [22], 'recombinant': [23], 'E.coli': [24], 'BL21': [25], 'harboring': [26], 'expression': [28], 'vector': [29], 'p': [31], 'hy': [32], 'C.': [33], 'Mutation': [34], 'residues': [35], 'were': [36, 55, 68, 77, 89, 95, 143], 'chosen': [37], 'based': [38], 'sequence': [41], 'alignments': [42], 'structure': [44], 'analysis': [45], 'neutral': [47], 'phytsaes': [48], 'different': [50, 75], 'microorganisms.': [51], 'Site-directed': [52], 'techniques': [54], 'used': [56], 'get': [58], 'three': [59], 'mutants': [60, 67, 76, 88], '(D148E/H149R,': [61], 'Q67E/N68R,': [62], 'D191E),': [64], 'then': [65], 'expressed': [69], 'purified.': [71], 'Enzymatic': [72], 'characters': [73], 'investigated.': [78], 'The': [79, 100, 120, 151], 'results': [80], 'indicated': [81], 'that': [82, 115, 131, 140], 'optimum': [84, 93], 'pH': [85], 'all': [87], '7.0,': [90], 'temperature': [94, 123], 'between': [96], '65': [97], '°C–70': [98], '°C.': [99], 'maximum': [101], 'specific': [102], 'mutant': [105, 149], 'D148E/H149E': [106], 'was': [107, 111, 126], '27.84': [108], 'U/mg': [109], 'which': [110, 155], '2.19': [112], 'times': [113], 'than': [114, 130], 'wild-type': [118, 134], 'phytase.': [119, 135], 'half': [121], 'inactivation': [122], 'D191E': [125], '4.5': [127], '°C': [128], 'higher': [129], 'Fluorescence': [136], 'emission': [137], 'spectra': [138], 'showed': [139], 'slight': [141], 'differences': [142], 'among': [144], 'structures': [146], 'phytases.': [150], 'phytases': [152], 'described': [153], 'here': [154], 'have': [156, 162], 'increased': [157], 'thermostability': [160], 'may': [161], 'promosing': [163], 'potential': [164], 'as': [165], 'feed': [166], 'additives': [167], 'in': [168], 'animal': [169], 'diets.': [170]}, 'cited_by_api_url': 'https://api.openalex.org/works?filter=cites:W1976087115', 'counts_by_year': [{'year': 2021, 'cited_by_count': 1}, {'year': 2020, 'cited_by_count': 1}, {'year': 2017, 'cited_by_count': 2}], 'updated_date': '2024-12-16T00:31:14.935737', 'created_date': '2016-06-24'}