Title: Affinity of pertussis toxin produced by Bordetella pertussis for human haptoglobin: application to the in vitro assay of the toxin
Abstract: Pertussis toxin formed a stable complex with human (Hp). Hemagglutinating activity of the toxin was inhibited in the presence of Hp, but leukocytosis activity was not. An enzyme linked immunosorbent assay for the toxin, Hp-ELIS, was developed on the basis of its specific affinity for Hp. A polystyrene microplate coated with Hp was incubated with samples containing the toxin. The bound was measured by sequential reaction with antipertussis toxin goat IgG, alkaline-labelled anti-goat IgG and p-nitrophenylphosphate. The Hp-ELISA method showed high specificity, high sensitivity and good correlation with leukocytes promoting activity in vivo. One ng of pertussis toxin could be detected within 3 h.
Publication Year: 1983
Publication Date: 1983-04-01
Language: en
Type: article
Indexed In: ['crossref']
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Cited By Count: 24
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