Title: ISOLATION, IDENTIFICATION, AND MEDIA OPTIMIZATION OF HIGH-LEVEL CELLULASE PRODUCTION BY<i>Bacillus</i>sp. BCCS A3, IN A FERMENTATION SYSTEM USING RESPONSE SURFACE METHODOLOGY
Abstract: Cellulases are important glycosyl hydrolase enzymes, which break down cellulose to β-glucose. They have been used widely in biotechnological processing such as bioethanol production. In this work we studied maximizing cellulase production by Bacillus sp. BCCS A3 using response surface methodology (RSM). A good result was attained with these conditions (% w/v): tryptone 0.1, Na₂PO₄ 0.25, (NH₄)₂SO₄ 0.2, MgSO₄ · 7H₂O 0.005, CaCl₂ 0.005, KH₂PO₄ 0.1, NaCl 0.1, sodium carboxymethylcellulose (CMC) 0.75, and pH 9. The cellulase activity in optimized medium was 49.80 U/ml. Moreover, high level of enzyme production was obtained by using fermentor system (50.30 U/ml). Thus, according to the obtained results, this statistical method provided quick identification and integration of key medium details for Bacillus sp. BCCS A3, leading to more cellulase production.
Publication Year: 2013
Publication Date: 2013-04-10
Language: en
Type: article
Indexed In: ['crossref', 'pubmed']
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Cited By Count: 15
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