Title: Digestive enzyme responses of tambaqui (Colossoma macropomum) fed on different levels of protein and lipid
Abstract: Aquaculture NutritionVolume 12, Issue 6 p. 443-450 Digestive enzyme responses of tambaqui (Colossoma macropomum) fed on different levels of protein and lipid L.C. DE ALMEIDA, L.C. DE ALMEIDA Federal University of Sao Carlos, Department of Genetics and Evolution, Sao Carlos, Sao Paulo, BrazilSearch for more papers by this authorL.M. LUNDSTEDT, L.M. LUNDSTEDT Federal University of Sao Carlos, Department of Genetics and Evolution, Sao Carlos, Sao Paulo, BrazilSearch for more papers by this authorG. MORAES, G. MORAES Federal University of Sao Carlos, Department of Genetics and Evolution, Sao Carlos, Sao Paulo, BrazilSearch for more papers by this author L.C. DE ALMEIDA, L.C. DE ALMEIDA Federal University of Sao Carlos, Department of Genetics and Evolution, Sao Carlos, Sao Paulo, BrazilSearch for more papers by this authorL.M. LUNDSTEDT, L.M. LUNDSTEDT Federal University of Sao Carlos, Department of Genetics and Evolution, Sao Carlos, Sao Paulo, BrazilSearch for more papers by this authorG. MORAES, G. MORAES Federal University of Sao Carlos, Department of Genetics and Evolution, Sao Carlos, Sao Paulo, BrazilSearch for more papers by this author First published: 02 November 2006 https://doi.org/10.1111/j.1365-2095.2006.00446.xCitations: 48 G. Moraes, Federal University of Sao Carlos, Department of Genetics and Evolution, Rod. Washington Luiz, Km 235, CEP 13565-905. Sao Carlos, SP, Brazil. E-mail: [email protected] Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract The adaptation of digestive proteases, amylase and lipase was studied in tambaqui fed with 350, 253, 301 and 205 g kg−1 of crude protein, and 49, 81, 113 and 145 g kg−1 of lipid, in isocaloric diets. Digestive protease increased when dietary protein increased in stomach, where the highest specific activity was observed. Unspecific protease activity in intestine was very low. Lipase was observed throughout the gastrointestinal tract, and higher activities were observed in the stomach. However, pyloric caecum and the anterior and posterior intestines were the responsive sections to the dietary lipid. Amylase was detected throughout the gastrointestinal tract, but pyloric caecum was the most relevant amylase producer. Positive correlations were observed between anterior intestine lipase versus dietary lipid and pyloric caecum amylase versus dietary protein. Tambaqui is responsive to the food composition adapting the main digestive enzymes to the best profile. References Albro, P.W., Hall, R.D., Corbett, J.T. & Schroeder, J. (1985) Activation of non-specific lipase (EC 3.1.1.) by bile salts. Biochem. Biophys. Acta, 835, 477– 490. Araújo-Lima, C. & Goulding, M. (1998) Os frutos do tambaqui: ecologia, conservação e cultivo na Amazônia. MCT-CNPq, Brasília, 186 p. Bazaz, M.M. & Keshavanath, P. (1993) Effect of feeding different levels of sardine oil on growth, muscle composition and digestive enzyme activities of mahseer, Tor Khudree. Aquaculture, 115, 111– 119. Bernfeld, P. (1955) Amylases α and β: colorimetric assay method. In: Methods in Enzymology ( S.P. Colowich & N.O. Kaplan eds), Vol. 1. Academic Press, New York. Bradford, M.M., McRorie, R.A. & William, W.L. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248– 254. Cataldi, E., Catandella, S., Monaco, G., Rossi, A. & Taucioni, L. (1987) A study of the histology and morphology of digestive tract of the sea-bream, Sparus aurata. J. Fish Biol., 30, 135– 145. Chakrabarti, I., Gani, M.D.A., Chaki, K.K., Sur, R. & Misra, K.K. (1995) Digestive enzymes in 11 freshwater teleost fish species in relation to food habit and niche segregation. Comp. Biochem. Physiol., A 112, 167– 177. Chesley, L.C. (1934) The concentrations of proteases, amylase, and lipase in certain marine fishes. Biol. Bull., 66, 133– 144. Das, K.M., Ghosh, A. & Ghosh, A. (1987) Studies on the comparative activity of some digestive enzymes in fry and adult of a mullet Liza parsia (Ham.). J. Aqua. Trop., 2(1): 9– 15. Deguara, S., Jauncey, K. & Agius, C. (2003) Enzyme activities and pH variations in the digestive tract of gilthead sea bream. J. Fish. Biol., 62, 1033– 1043. Divakaran, S., Kim, B.G. & Ostrowski, A.C. (1999) Digestive enzymes present in Pacific threadfin Polydactylus sexfilis (Block an Schneider 1801) and bluefin trevally Caranx melampygus (Cuvier 1833). Aquacult. Res., 30, 781– 787. Elbal, M.T. & Agulleiro, B. (1986) A histochemical and ultrastructural study of the gut of Sparus aurata (Teleosti). J. Subm. Cytol., 18, 335– 347. Ellis, S.C. & Reigh, R.C. (1991) Effects of dietary lipid and carbohydrate levels on growth and body composition of juvenile red drum (Sciaenops ocellatus). Aquaculture, 97, 383– 394. Fagbenro, O., Adhdirh, C.O, Ayotunde, E.O. & Famini, E.O. (2000) Haematological profile, food composition and digestive enzyme assay in the gut of the African bony-tongue fish, Heterotis (Clupisudis) niloticus (Cuvier 1829) (Osteoglossidae). Trop. Zool., 13, 1– 9. Gangadhar, B., Nandeesha, M.C., Varghese, T.J. & Keshavanath, P. (1997) Effect of varying protein and lipid levels on the growth oh rohu, Labeo rohita. Asian Fish Sci., 10, 139– 147. Goulding, M. (1980) The Fish and the Forest-Explorations in Amazonian Natural History. California: University of California, 280 p. Goulding, M. & Carvalho, M.L. (1982) Life history and management of the tambaqui (Colossoma macropomum, Charachidae). An important Amazonian food fish. Revista Brasileira de Zoologia, 1, 107– 133. Hepher, B., Shilo, M. & Shmuelsarig (1989) Principles of fish nutrition. In: Fish Culture in Warm Water Systems: Problems and Trends, pp. 121– 141. CRC, Boca Raton. Hidalgo, M.C., Urea, E. & Sanz, A. (1999) Comparative study of digestive enzymes in fish with different nutritional habits. Proteolytic and amylase activities. Aquaculture, 170, 267– 283. Honda, E.M.S. (1974) Contribuição ao conhecimento da biologia de peixes no Amazonas. II. Alimentação do tambaqui, Colossoma bidens. Acta Amazônica, 1, 47– 53. Hsu, Y.L. & Wu, J.L. (1979) The relationship between feeding habits and digestive proteases of some freshwater fishes. Bull. Inst. Zool. Acad. Sinica, 18, 45– 53. Jany, K.D. (1976) Studies on the digestive enzymes of the stomachless bonefish Carassius auratus gibelio (Bloch): endopeptidases. Comp. Biochem. Phys., 53, 31– 38. Kitamikado, M. & Tachino, S. (1960) Studies on the digestive enzymes of rainbow trout: II. Proteases. Bull. Jpn. Soc. Sci. Fish, 26, 685– 690. Koven, W.M., Henderson, R.J. & Sargent, J.R. (1997) Lipid digestion in turbot (Scophthalmus maximus): in vivo and in vitro studies of the lipolytic activity in various segments of the digestive tract. Aquaculture, 151, 155– 171. Krogdahl, A, Hemre, G.I. & Mommsen, T.P. (2005) Carbohydrates in fish nutrition: digestion and absorption in postlarval stages. Aquacult. Nutr., 11, 103– 122. Kuz'mina, V.V. (1990) Temperature influence on the total level of proteolytic activity in the digestive tract of some species of freshwater fishes. J. Ichthyol., 30, 97– 109. Kuz'mina, V.V. (1991) Evolutionary Features of the Digestive-Transport Function in Fish, Vol. 27. pp. 167– 175. Plenum Publishing Corporation, translated from Zhurnal Evoyutsionnoi Biokhimii I Fiziologii. Lemieux, H., Blier, P. & Dutil, J.D. (1999) Do digestive enzymes set a physiological limit on growth rate and food conversion efficiency in the Atlantic cod (Gadus morhua)? Fish Phys. Biochem., 20, 293– 303. Lundstedt, L.M., Melo, J.F.B. & Moraes, G. (2004) Digestive enzymes and metabolic profile of Pseudoplatystoma corruscans (Teleostei: Siluriformes) in response to diet composition. Comp. Biochem. Physiol., B 137, 331– 339. Manjappa, K., Keshavanath, P. & Gangadhara, B. (2002) Growth performance of common carp, Cyprinus carpio fed varying lipid levels through low protein diet, with a note on carcass composition and digestive enzyme activity. Acta Ichtyol. Et Piscat. XXXII, Fasc., 2, 146– 155. Moraes, G. & Bidinotto, P.M. (2000) Induced changes in the amylohrydrolytic profile of the gut of Piaractus mesopotamicus (Holmberg 1885) fed different levels of soluble carbohydrate: its correlation with metabolic aspects. Revista de Ictiologia, 8, 47– 51. Olatunde, A.A. & Ogunbiyi, O.A. (1977) Digestive enzymes in the alimentary tracts of three tropical catfish. Hydrobiology, 56, 21– 24. Omar, E., Nour, A.M. & Guenther, K.D. (1986) Nutritional requirements of common carp (Cyprinus carpio L.). Anim. Res. Dev., 23, 80– 95. Overnell, J. (1973) Digestive enzymes of the pyloric caeca and of their associated mesentery in the cod (Gadus morhua). Comp. Biochem. Physiol., 46, 519– 531. Park, J.T. & Johnson, M.J. (1949) A submicro determination of glucose. J. Biol. Chem., 181, 140– 151. Peres, H. & Olíva-Teles, A. (1999) Effect of dietary lipid level on growth performance and feed utilization by European sea bass juveniles (Dicentrarchus labrax). Aquaculture, 179, 325– 334. Reimer, G. (1982) The influence of diet on the digestive enzymes of the amazon fish Matrincha, Brycon cf melanopterus. J. Fish Biol., 21, 637– 642. Sabapathy, U. & Teo, L.H. (1993) A quantitative study of some digestive enzymes in rabbitfish, Siganus canaliculatus and the sea bass, Later calcarifer. J. Fish Biol., 42, 595– 602. Sallum, W.B., Bertechini, A.G., Cantelmo, O.A., Pezzato, L.E. & Logato, P.R.V. (2002) Apparent digestibility coefficients of ingredients of ration for the matrinchã (Brycon cephalus, Gunther 1869) (Teleostei, Characidae). Ciênc. Agrotec., Larvas, 26, 174– 181. Sarath, G., De La Motte, R.S. & Wagner, F.W. (1989) Protease assay methods. In: Proteolitic Enzymes. A Practical Approach ( R.J. Beymon & J.S. Bond eds), pp. 25– 55. IRL Press, Oxford. Schneider, B.H. & Flatt, W.P. (1975) The Evaluation of Feeds Through Digestibility Experiments. The University of Georgia Press, Athens, USA. Seixas, J.T.F., Oliveira, M.G.A., Donzele, J.L., Gomide, A.T.M. & Menin, E. (1999) Atividade de amilase em quimo de três espécies de peixes Teleostei de água doce. Rev. Bras. Zootec., 28, 907– 913. Silva, J.A.M., Pereira-Filho, M. & Oliveira-Pereira, M.I. (2003) Frutos e sementes consumidos pelo tambaqui, Colossoma macropomum (Cuvier, 1818) incorporados em rações. Digestibilidade e velocidade de trânsito pelo trato gastrointestinal. Revista Brasileira de Zootecnia, 32, 1815– 1824. Tengjaroenkul, B., Smith, B.J., Caceci, T. & Smith, S.A. (2000) Distribution of intestinal enzyme activities along the intestinal tract of cultured Nile tilapia, Oreochromis niloticus L. Aquaculture, 182, 317– 327. Val, A.L. & Honczaryk, A. (1995) Criando peixes na Amazônia. INPA, Manaus, 160 p. Walter, H.E. (1984) Proteinases: methods with hemoglobin, casein and azocoll as substrates. In: Methods of Enzymatic Analysis ( H.U. Bergmeyer ed.), Vol. 5. pp. 270– 277. Verlag Chemie, Weinheim. Citing Literature Volume12, Issue6December 2006Pages 443-450 ReferencesRelatedInformation